tổng chi phí tổng chi phí phát triển phần mềm bảo trì hàng năm 116 5 5 121 5 triệu

Báo cáo khoa hoc:" Genetic polymorphisms and susceptibility to lung disease" ppsx

Ngày tải lên : 11/08/2014, 08:20

... GTTTCTGCTGCAGCGCC CACCTCCATGATTCTGA CACCTCCAGGATTCTGA 62 54 56 60 63 58 56 58 56 56 54 56 52 53 55 Temp °C 58 60 60 53 53 55 53 50 48 Temp °C 60 54 60 58 52 54 Page of 11 (page number not for citation purposes) ... TCGTGGCCACAGCTGGG TCGTGGCCGCAGCTGGG 60 Temp °C 60 60 60 60 53 53 62 61 58 56 57 55 60 60 60 60 55 53 57 55 47 47 50 50 54 54 57 57 61 63 60 60 60+DMSO 59 59 Page of 11 (page number not for citation purposes) ... CCATCGACAAGAAAGGG 58 60 57 55 60 58 Temp °C 60 65 60 56 54 50 50 56 54 Page of 11 (page number not for citation purposes) Journal of Negative Results in BioMedicine 2006, 5: 5 Table 2: Pseudogene...

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Báo cáo khoa học: "Expression and characterization of the flavoprotein domain of gp91phox" potx

Ngày tải lên : 07/08/2014, 14:22

... 196(2), 54 3 -52 39 Rotrosen D, Kleinberg ME, Nunoi H, Leto T, Gallin JI, Malech HL Evidence for a functional cytoplasmic domain of phagocyte oxidase cytochrome b 558 J Biol Chem, 1990, 2 65( 15) , 87 45- 50 ... between p47-phox and cytochrome b 558 J Clin Invest, 1991, 87(1), 352 -6 21 Imajoh-Ohmi S, Tokita K, Ochiai H, Nakamura M, Kanegasaki S Topology of cytochrome b 558 in neutrophil membrane analyzed ... USA, 1998, 95( 14), 7993-8 55 Zhou Y, Lin G, Murtaugh MP Interleukin-4 suppresses the expression of macrophage NADPH oxidase heavy chain subunit (gp91-phox) Biochim Biophys Acta, 19 95, 12 65( 1), 40-8...

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Báo cáo khoa học: "Activation domain in P67phox regulates the steady state reduction of FAD in gp91phox" pot

Ngày tải lên : 07/08/2014, 14:22

... flavocytochrome b 558 , 800 nM p47phox, 450 nM Rac1, 900 nM of truncated p67phox, and 0.2 mM arachidonate The fluorescence emission spectrum of 52 5 nm (excitation wavelength, 4 75 nm) was recorded ... Blood 1996, 87 (5) , 166381 Rotrosen D, Yeung CL, Leto TL, Malech HL, and Kwong CH Cytochrome b 558 : the flavin-binding component of the phagocyte NADPH oxidase Science 1992, 256 (50 62), 1 459 -62 Rotrosen ... flavocytochrome b 558 that had been reconstituted with FAD or FAD analog and phospholipids, 800 nM p47phox, 900 nM p67phox, 450 nM Rac, 10 mM GTPgS, and 200-240 mM arachidonate in a total of 50 ml Three...

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Báo cáo y học: "A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity" doc

Ngày tải lên : 09/08/2014, 10:21

... 181,791,321 to 181,826,339 15 NCF4 22 35, 586,991 to 35, 604,004 10 CYBB X 37 ,52 4,264 to 37 ,55 7, 658 13 CYBA 16 87,237,199 to 87,244, 958 RAC2 22 35, 951 , 258 to 35, 970, 251 Positions according to National ... or in the EIRA cohort (rs1804006 [CYBA], rs789183, rs1330 658 1 and rs1330 657 5 [NCF2], and rs13 057 803, rs100 350 1, rs12 158 689, rs961 059 5, rs2072706 and rs2072711 [NCF4]; Additional file 1) Fourteen ... OR 95% CI P All 0.80 0.66 to 0.97 0.02 1.04 0.67 to 1.62 0.86 0.94 0. 75 to 1.17 0 .56 1.01 0.60 to 1.71 0.97 0 .50 0. 35 to 0.73 0.0002 1.20 0 .52 to 2.81 0.67 All 0. 85 0.69 to 1.04 0.12 0.92 0 .56 ...

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Báo cáo khoa học: "Long Sentence Analysis by Domain-Specific Pattern Grammar" pptx

Ngày tải lên : 09/03/2014, 01:20

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Báo cáo y học: "DEK binding to class II MHC Y-box sequences is gene- and allele-specific" pptx

Ngày tải lên : 09/08/2014, 01:23

... polymerase (Invitrogen) was added at 80°C after a 94°C ‘hot start,’ initiating 35 cycles of PCR: 94°C for 45 s, 55 °C for 30 s, and 72°C for 90 s, with final extension at 72°C for 10 PCR products ... interact DQA1* 050 1 CTAATTAGCC 58 2 ± 130 DRA CTGATTGGCC 2480 ± 51 9 DQB consensus CTGATTGGTT 1977 ± 93 DRB consensus CTGATTGGTT 1266 ± 274 Discussion DRB-DR4 CTGATTCGTT 1041 ± 258 All antigen-presenting ... arthritis (JCA): search for a specific autoantibody associated with JCA Ann Rheum Dis 1991, 50 :59 5 -59 8 Szer W, Sierakowska H, Szer IS: Antinuclear antibody profile in juvenile rheumatoid arthritis...

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Báo cáo y học: " Non-imprinted allele-specific DNA methylation on human autosomes" pptx

Ngày tải lên : 09/08/2014, 20:21

... templates for PCR by using the following thermal cycling parameters: 15 minutes at 95 C, 35 cycles of 45 s at 95 C, 35 s at 55 °C and 50 s at 72°C and finally 72°C for minutes The primers were designed ... digested genomic DNA using the following thermal cycling parameters: 15 minutes at 95 C, 35 cycles of 45 s at 95 C, 45 s at 50 °C and minute at 72°C, and finally 72°C for minutes The sequences of ... 400 241 302 476 CpG sites 31 26 42 25 31 44 A/C (-) rs2297246 A/C (+) rs56270809 A/G (-) rs2838343 A/G (+) rs 558 60816 del/no del (+) rs4 156 30 15 C/G (+) rs 256 78 Individuals studied 38 19 33 36...

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Báo cáo y học: " Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity" ppt

Ngày tải lên : 09/08/2014, 23:20

... 353 4.1 4263 51 26 2.8 652 0 3.1 68 3.9 6969 Figure 2 Additional files provided with this submission: Additional file 1: Additional_File_1.pdf, 1302K http://genomebiology.com/imedia/963439 856 59 250 45/ supp1.pdf ... http://genomebiology.com/imedia/963439 856 59 250 45/ supp1.pdf Additional file 2: Additional_File_2.pdf, 2402K http://genomebiology.com/imedia/177271718 459 250 5/supp2.pdf ... Uppsala University, Rudbeck Laboratory, SE- 751 85 Uppsala, Sweden Department of Surgical Sciences, Uppsala University, Akademiska sjukhuset, SE- 751 85 Uppsala, Sweden Department of Radiology,...

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Báo cáo y học: "A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients" pdf

Ngày tải lên : 10/08/2014, 21:23

... 100%, 50 %, 25% , 12 .5% , and 6. 25% A “C” peak which represented the WT BCR-ABL KD allele could be seen in 50 %, 25% , 12 .5% , 6. 25% , 3.13%, and 0% dilution (100% WT) (Figure 2B) 3.3 Detection for T315I ... T315I peak had a different peak pattern from other common mutations (Y 253 F, Y 253 H, E 255 K, M 351 T, and F 359 V) (data no shown) For direct sequencing, a “T” peak indicated the presence of T315I which ... temperature (Ta) (55 ° to 62°C), MgCl2 concentration (1.0-2 .5 mmol/L), and primer ratios (MT: WT ratio of 8:2, 7:3, 6:4, and 5: 5) Briefly, the optimized condition was performed in a 25- μL mixture of...

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Báo cáo y học: "Analysis of adenovirus trans-complementationmediated gene expression controlled by melanoma-specific TETP promoter in vitro" ppsx

Ngày tải lên : 12/08/2014, 04:20

... 19 95, 1:9 85- 1000 Fontecedro et al Virology Journal 2010, 7:1 75 http://www.virologyj.com/content/7/1/1 75 Page 17 of 17 61 Zhang Y, Bergelson JM: Adenovirus receptors J Virol 20 05, 79 :121 25- 1213 1 ... Gene Ther 20 05, 5: 411-427 47 Dorer DE, Nettelbeck DM: Targeting cancer by transcriptional control in cancer gene therapy and viral oncolysis Adv Drug Deliv Rev 2009, 61 :55 4 -57 1 48 Mizuguchi H, Xu ... 1994, 91:4407-4411 57 Siders WM, Halloran PJ, Fenton RG: Transcriptional targeting of recombinant adenoviruses to human and murine melanoma cells Cancer Res 1996, 56 :56 38 -56 46 58 Nettelbeck DM,...

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Báo cáo y học: " Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene" pdf

Ngày tải lên : 13/08/2014, 05:21

... -actin F Ct HMBOX1 Exo1 -actin Ct /E + + 15 143b 143b +Tat E Ct UP5 20 + + T 15 A4 A4 + Tat E D -actin 40 35 30 25 20 15 10 E E Ct START /E Ct -actin C - - M T5 - HOS_143b + HOS_A4 - + - + - + - -RT ... of 15 (page number not for citation purposes) Retrovirology 2008, 5: 98 http://www.retrovirology.com/content /5/ 1/98 HOS_A4 + tat HIV-1 probe HMBOX1 probes Tat-EGFP DAPI Cy3/Cy5 merge EGFP/Cy5 merge ... loop-specific binding to TAR RNA Cell 1998, 92: 451 -462 http://www.retrovirology.com/content /5/ 1/98 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 Marcello A, Lusic M, Pegoraro G,...

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PHÂN BIỆT GENOTYPE 1 và 6 BẰNG kỹ THUẬT ALLELE SPECIFIC RT PCR dựa vào VÙNG GEN NS5B của VIRUS VIÊM GAN c

Ngày tải lên : 20/08/2015, 11:28

... 1b 6 15 6f 623 6f 652 6e (4) 1 6 (1) 17 18 19 20 21 22 (2) 141 CC3 35 CC337 CC484 CC4 85 670 (3) 1b 6a 6e 1b 1b 6a (4) 6 1 (1) (2) (3) (4) 33 CC 154 4 6e 34 CC 159 1 6e 35 CC 159 2 1a 36 54 4 6p 37 56 7 ... phản ứng I với hệ mồi nhằm phát genotype (NS5B16F-NS5B1R) phản ứng II với hệ mồi nhằm phát genotype (NS5B16F-NS5B6R) Chơng trình luân nhiệt cho phản ứng PCR nh sau: 95oC- 15 phút (1 chu kỳ); 94oC-1 ... 11 12 13 14 15 16 CC34 CC 35 CC36 CC 151 CC 152 CC 153 CC208 CC179 CC2 65 113 1a 6a 6e 6e 6a 1b 6e 6p 6e 1b 6 6 6 23 671 24 672 25 674 26 818 27 847 28 241 29 283 30 291 31 972 32 CC 154 3 6e 1a 6a...

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Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc

Ngày tải lên : 23/03/2014, 09:21

... (2007) 1862–1877 ª 2007 The Authors Journal compilation ª 2007 FEBS 18 75 Modeling of a-syn oligomer formation 52 53 54 55 56 57 58 59 60 61 62 63 I F Tsigelny et al tion of secondary structure in natively ... with 1 .5 ns molecular dynamics a-syn conformer docked on the ﬂat membrane MD, molecular dynamics b-syn conformer MD time (ns) Electrostatic energy (kcalÆmol)1) Initial 0. 25 0 .50 0. 75 1.00 1 .50 )27.4 ... involved in neurodegeneration [52 ], including amyloid-b protein [53 ], British dementia peptide (ABri) [54 ], Danish dementia peptide (ADan), serum amyloid A, and amylin [55 ] In such studies, the dimension...

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Nghiên cứu tính đa dạng của allele hla - DQA1 bằng kỹ thuật polymerase chain reaction sequence specific primers (PCR - SSP) ở dân tộc kinh miền trung - Việt Nam docx

Ngày tải lên : 25/03/2014, 03:22

... 48 Tần suất kháng nguyên (%) 0. 35 0.29 0.04 0. 45 0.11 0.11 0.03 0.10 0.03 0.02 Tần suất allele tìm đợc (%) 0.194 0. 157 0.020 0. 258 0. 056 0. 056 0.0 15 0. 051 0.0 15 0.010 Chúng phân bố khác cá thể ... 0301 A * 5' 05 + A * 3'03 170bp DQA1 *0301 DQA1 * 0302 A * 5' 06 + A * 3'03 183bp DQA1 *0302 DQA1 * 0401 A * 5' 07 + A * 3'04 190bp DQA1 *01030401 DQA1 * 050 1 A * 5' 02 + A * 3' 05 186bp DQA1 * 050 1 10 ... tâm 350 0 rpm /5 phút, bỏ phần dịch phía trên, thêm 2ml dung dịch TES 2mm, trộn đều, thêm 0,5ml dung dịch 10% SDS, trộn đều, thêm 2ml dung dịch Phenol bão hoà TES Trộn đều, ly tâm 350 0 rpm /5 phút,...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... and IFN-γ gene expression (r = 0. 854 p = 0.001 and r = 0.629, p = 0.012 respectively) induced by HCMV pp 654 95 50 3, EBV BMLF-1 259 – 267, EBV LMP-2426–434 and IM58–66 HLA-A0201 restricted peptides ... http://www.translational-medicine.com/content/6/1 /58 -4 > > > > > > > > > r = 0.37 p = 0.001 > -3 > > > > >> > >> > -2 IFN-Ȗ rel.exp (log10) > -1 > > > > -2 .5 -2.0 > > > > > >> > > > > > > > > > > r = 0 .55 p = 0.000001 -1 .5 -1.0 -0 .5 TNF-Į ... μl) was incubated for hours in the presence of HCMV pp 654 95 50 3 (triangles), EBV LMP-2426–434 (diamonds), BMLF-1 259 –267 (squares) and IM58–66 virus (crosses) derived peptides at 10 μg/ml final...

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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot

Ngày tải lên : 18/06/2014, 16:20

... Female 82 25 190 63 Male 253 75 83 56 Never 15 19 43 Current 2 15 64 NR 60 Former 1 05 31 71 54 197 59 NR 63 ECOG 120 36 64 52 ECOG 18 25 33 10% 32 10 98 57 191 95 57 34 NR 47 ... 157 47 190 71 II 136 41 61 51 IIIa 42 12 17 23 85 188 25 56 190 84 74 57 62 19 25 36 232 69 190 66 76 23 35 43 27 18 18 307 28 92 190 47 58 47 No 284 85 190 58 Yes 51 15 27 32 0.004 Pathological ... 133 64 High 98 51 120 68 Missing SCC, N0 Low 59 47 160 79 High 68 53 129 67 Low 30 50 49 32 High 30 50 95 NS 58 Missing Multivariate P 59 High Uni-variate P 0.43 Total (n = 3 35) 68 SCC (n = 191)...

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báo cáo hóa học:" Transcriptional regulation of bone formation by the osteoblast-specific transcription factor Osx Chi Zhang" pptx

Ngày tải lên : 20/06/2014, 04:20

... Foundation (To Chi Zhang) and RAP01 grant from Texas Scottish Rite Hospital for Children (To Chi Zhang) Author Details Bone Research Laboratory, Texas Scottish Rite Hospital for Children, Department ... Accepted: 15 June 2010 Published: 15 June 2010 © 2010 Zhang; Access Surgery and Research 2010, 5: 37 of the Creative This is an Openlicenseefrom: http://www.josr-online.com/content /5/ 1/37 Commons ... Dallapiccola B, De Paepe A, et al.: LDL receptor-related protein (LRP5) affects bone accrual and eye development Cell 2001, 107 :51 3 -52 3 15 Kato M, Patel MS, Levasseur R, Lobov I, Chang BH, Glass DA,...

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báo cáo hóa học:" How do existing HIV-specific instruments measure up? Evaluating the ability of instruments to describe disability experienced by adults living with HIV" pot

Ngày tải lên : 20/06/2014, 16:20

... Care 2003, 15( 3):347- 357 54 Lenderking WR, Testa MA, Katzenstein D, Hammer S: Measuring quality of life in early HIV disease: the modular approach Qual Life Res 1997, 6(6) :51 5 -53 0 55 Holzemer ... General Health Self Assessment [54 ] Lenderking et al HRQL/QOL 1997 50 32 Living with HIV Scale [55 ] Holzemer et al HRQL/QOL 1998 HIV Cost and Services Utilization Tool [56 ] Hays et al HRQL/QOL 1998 ... Care 1993, 31(7) :56 9 -58 0 19 Wilson IB, Cleary PD: Linking clinical variables with health-related quality of life A conceptual model of patient outcomes Jama 19 95, 273(1) :59 - 65 20 Finch EP, Brooks...

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Báo cáo hóa học: " Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report" doc

Ngày tải lên : 21/06/2014, 19:20

... in 55 % of Rubinstein–Taybi syndrome patients, leaving the diagnosis in 45% of patients to rest on clinical features only Interestingly, this microdeletion of 16p13.3 was found in a young child ... in 100,000 to 1 25, 000 [2] About 55 % of patients have cytogenetic or molecular abnormalities in the Crebbp or E1A binding protein p300 (Ep300) gene, leaving the diagnosis in 45% of patients to ... retardation syndrome Am J Dis Child 1963, 1 05: 588-608 Hennekam RCM, Stevens CA, Van de Kamp JJ: Etiology and recurrence risk in Rubinstein–Taybi syndrome Am J Med Genet Suppl 1990, 6 :56 -64 3 Hennekem RCM:...

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Báo cáo y học: "Specific immunotherapy by the sublingual route for respiratory allergy" pot

Ngày tải lên : 08/08/2014, 21:20

... compliance rate higher than 75% was found in 85% of the children at the third month and in 84% of the children at the sixth month and the major cause of withdrawal (5. 6% of the cases) was the cost ... of sublingual-swallow immunotherapy: an analysis of published studies Clin Exp Allergy 20 05, 35: 5 65- 571 de Groot H, Bijl A: Anaphylactic reaction after the first dose of sublingual immunotherapy ... than a SCIT-treated patient for all out-of-pocket costs (€ 176 vs € 255 ) but paid more for allergen extracts (€ 72 vs € 55 ) The sum of direct and indirect costs gave, over the 3-year treatment,...

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