... shed light on factors involved in directing lymphocytes into inflamed tissue and maintaining inflammation in SS, whereas the etiologic factors of SS remain to be delineated Of potential importance ... expressing VH1-69/DP10 cross-reactive idiotypes G6, G8 and H1 are increased in infiltrates in the minor salivary glands of patients with pSS [65] Support for a role of B cell activation in the ... cells in the parotids, together with new findings of the role of chemokines and chemokine receptors, permitted new insight into the immunopathogenesis of pSS Although most the current data indicate...
... take into account these two distinct cellular types Imatinib is a tyrosine kinase inhibitor that specifically binds the ATP pocket of Bcr-Abl tyrosine kinase, inhibiting the activity of the kinase ... activation of phosphatidylinositol 3-kinase (PI-3K) and (2) Jak-Stat kinases In addition, Bcr-Abl can affect p53 and MYC in a RAS-dependent manner [12-14] and can activate Jun N-terminal kinase (JNK) In ... effect of the Bcr-Abl inhibitor Imatinib correlated most closely with the inhibition of PI-3K within chronic myeloid leukemia progenitor cells, and also found that AG490, a Jak2 kinase inhibitor and...
... β-catenin in the presence and absence of 5µM AKT inhibitor IV 85 3.30 Lack of effects of FAK inhibitor on expression levels of E-cadherin 86 XII and β-catenin 3.31 Intracellular Ca2+ measurement indicates ... carboxyl-terminal tail as illustrated in Figure 1.5C appears to be the docking site for at least two interacting sites including calmodulin (CAM) binding sites and region involved in microfilament-associated ... TRPV4 in the BCM cell lines 51 3.7B Immunofluorescence (IF) of TRPV4 in the BCM cell lines 51 3.8 52 The expressionof TRPV4 in BCM model was examined using realtime PCR X 3.9A Immunoblotting of...
... MO, USA) Intracellular staining for cytokines was performed using BD cytofix/cytoperm and BD perm/wash reagents with GolgiStop as recommended by BD Pharmingen Intracellular cytokine staining was ... smoke-induced inflammation andexpressionof chitinases and chitinase-like molecules To investigate the impact of cigarette smoke exposure on chitinase expression, BALB/c, C57BL/6, and CD1 mice ... the inflammatory process and induction of BRP-39 in a model of smoke exposure, we sought to investigate whether IL-13 was essential for the induction of BRP-39 in models of allergic airway inflammation...
... genotypes D and E respectively (Table 2) Four of the sera (7.8 %) were co-infected by genotypes B and C Table 2: Combination of genotyping results determined using methods developed by Lindh ... with genotype B being more predominant We had indeed observed that genotypes B and C were the main strains infecting HBV carriers in Malaysia with the former being more predominant However region-wise, ... subtypes exist namely Bj which is found mainly in Japan and Ba which is found in some other Asian countries such as China, Hong Kong, Taiwan, Thailand and Vietnam The Ba strain has recombination...
... N-acylsulfonamide-linked dinucleoside inhibitors of RNase A [1], and has numerous interesting homologs [24] In humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an important role in ... uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates In UpG, the binding of the guanine moiety is deterred by exocyclic O6 Close inspection shows ... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea...
... 2000 in blocking solution for 30 Cells were rinsed in NaCl ⁄ Pi several times and incubated in blocking serum containing anti-mouse IgG (Vectastain) for a further 30 Staining was developed by incubation ... Drakenberg T, Thulin E & Forsen S (1986) Expressionof bovine intes- Expressionand purification of the amyloid b-peptide 44 45 46 47 48 49 tinal calcium binding protein from a synthetic gene in Escherichia ... peroxidases were blocked in 0.3% H2O2, rinsed in NaCl ⁄ Pi and incubated in blocking solu- Expressionand purification of the amyloid b-peptide tion for 20 (Vectastain) Neurons were then incubated with...
... side chain of Lys9 and Hyp10 in forming the tight loop as in conomarphin The side chain of Lys9, Hyp10 and D-Phe13 of conomarphin is shown in stick mode, and the side chain of L-Phe13 in L-Phe13-conomarphin ... D-amino acid-containing conomarphin Y Han et al Fig Stereo view of the superimposed structures of conomarphin (red) and L-Phe13-conomarphin (gray) The side chain of L-Phe13 might cause spatial hindrance ... A D-amino acid-containing conomarphin Y Han et al refined for simulated annealing and restrained energy minimization [24,28], using the sander module of the amber 9.0 package The resulting conformers...
... pancreatic juice and intestine: inadequate for role in zinc absorption Am J Clin Nutr 35, 1–9 Evans GW & Johnson PE (1980) Characterization and quantitation of a zinc binding ligand in human milk ... splicing of exons andof the ACMSD gene (Fig 2A); the presence of exon in ACMSD II causes a shift in the open reading frame, resulting in the occurrence of the first available start codon in exon ... Residues involved in catalysis in the bacterial enzyme and the corresponding amino acids in the human protein are pointed with arrows The mean hydrophobicity values of residues within the window...
... most cystatins inhibiting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported The expressionof the cystatin F (CST7) and C (CST3) ... cystatin F, through analysis of the expression, distribution and properties of the native human inhibitor in U937 cells MATERIALS AND METHODS In silico cloning and sequencing of the human cystatin ... in the ER staining where cystatin F stains Regulation of cystatin F expression To shed light on the regulation of the cystatin F gene, we examined the sequence of the human gene, CST7 At an initial...
... structural integrity of the active FLS, point mutations were initiated aiming at the substitution of glycine residues by alanine or proline and the exchange of proline by glycine Glycine residues ... substitution of glycine by alanine and proline, respectively, or of proline by glycine (Table 1) Each individual mutation was verified by DNA sequencing using the dideoxynucleotide chain-termination ... site-directed mutagenesis of FHT [7,9] and by documentation of ligand binding in crystalline DAOCS and IPNS complexes [23–25] The coordination of FeII is commonly mediated by two histidine and one aspartate...
... adding 0.4 mL of staining solution [1% dinitrosalicylic acid (w/v) and 30% sodium potassium tartrate (w/v) in 0.4 M NaOH] and heating at 90 8C for before measuring the absorbance at 540 nm against ... raised against b-amylase and the unglycosylated isoform of CalsepRRP [14] Male New Zealand white rabbits were injected with mg purified protein dissolved in KCl/NaCl/Pi and emulsified in mL of Freund’s ... sepium using the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low...
... reported in a few in human gastrointestinal cancer tissues, thus sug- gesting the involvement of the variant in malignant phenotypes of certain cancers [17–20] However, because of its initial ... stage-dependent expressionof WISP1v in vitro Effect of overexpression of CCN4/WISP1 and its variants on the phenotypes of human chondrocytic HCS-2 ⁄ cells Fig Immunohistochemical staining of CCN4 ⁄ WISP1 and ... function of CCN4 ⁄ WISP1 variants Structure, expressionand function of the CCN4 ⁄ WISP1 variants are summarized All of the findings were obtained in this study except for that of gastrointestinal cancers...
... staining was performed using a Glycoprotein staining kit (Pierce, Rock, IL, USA) according to the manufacturer’s instruction Lectin-binding assays The ability of Cyn d 24 to bind various lectins ... the left of the figure indicate the positions of the deduced amino acid sequence N-terminal segment determined by protein sequencing is underlined (B) Comparison of the amino acid sequence of Cyn ... Using 2D electrophoresis and immunoblotting techniques, our recent studies have indicated that BGP contains about 230 proteins including isoforms ⁄ degraded protein products and around 65 of...
... & Zhao, S (2000) Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA Biochem J 349, ... protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-specific differences in the potencies of murine protein-kinase inhibitors ... endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse J Biol Chem 268, 10927–10931 Olsen, S.R & Uhler, M.D (1991) Inhibition of protein kinase-a by overexpression of the cloned...
... Sepharose, and the bound GST fusion protein was treated with mL of a thrombin digestion buffer (50 mM Tris/HCl pH 8.0, 150 mM NaCl, 2.5 mM CaCl2) containing UÆmL)1 bovine thrombin Following a 30-min incubation ... tyrosineO-sulphate-binding protein and tyrosine-sulphated proteins in bovine liver membrane lysates Biochem J 275, 259–262 16 Sanger, F., Nicklen, S & Coulson, A.R (1977) DNA sequencing with chain-terminating ... fetal bovine serum, 0.5% trout serum, 0.1 mgÆmL)1 bovine insulin, 50 lgÆmL)1 streptomycin sulfate, and 30 lgÆmL)1 penicillin G Confluent zebrafish liver cells grown in individual wells of a 24-well...
... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... motif of an adjacent subunit and thus contributes to stabilizing the intersubunit interface Indeed, the Arg297 and Gln304 side chains of the Ca8 helix make favorable interactions with the Glu422 and ... or low levels of expression) [18] Being embodied in homo- or heterotetrameric proteins, the resulting enzyme may inuence the overall in vivo activity [18] In vivo, the patient bearing mutation...
... Alignment of the amino acid sequences of nitrile hydratases (NHases) Three cysteine residues and one serine residue in the cysteine cluster region, two conserved arginine residues, and one conserved ... substitutions in Co-type NHase Co-type NHase contains threonine and tyrosine in the -C(T/S)LCSC(Y/T)- sequence of the active center, whereas Fe-type NHase contains serine and threonine (Fig 1) ... mutational andstructural analysis of the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile,...
... Concluding remarks The above results document the strong in uence of standard variables (pH of the solution, reaction time, and binding Table In uence of the reaction time (t) and binding ability of ... Notwithstanding this, there is a minor effect of t and X at the beginning and end of the titration Thus, the addition of the first 4–6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable ... metallothioneins containing mercury, lead and bismuth Inorg Chim Acta 79, 154– 155 25 Li, H & Otvos, J.D (1996) 111Cd NMR studies of the domain specificity of Ag+ and Cu+ binding to metallothionein Biochemistry...
... signal peptides of all the proteins were 16 amino acids long The mature proteins consisted of 122 amino acids for ammodytin I1, the A and B chains of vaspin and V b berus PLA2, and 121 amino acids ... (monomeric ammodytins A, B and C, and ammodytin I1n with a 259 bp intron D, the Bov-B LINE retroposon) andof V a aspis (vaspin A and B chains and ammodytin I1a with a 133 bp intron D; Table 2) ... ammodytoxin C, ammodytins I1 and I2 and ammodytin L genes of V am ammodytes, the VP7 and VP8 genes of V palaestinae, and the PLA2 genes of Crotalinae snakes (Trimeresurus flavoviridis) were also of...