trips and pitfalls in making and reding maps of carnivore distribution
Tài liệu Carnivore Ecology and Conservation A Handbook of Techniques pptx
Ngày tải lên :
17/02/2014, 17:20
... measures of precision and their meaning 2.4.6 Field methods Mind the map: trips and pitfalls in making and reading maps of carnivore distribution Carlo Rondinini and Luigi Boitani 3.1 Maps based ... map: trips and pitfalls in making and reading maps of carnivore distribution Carlo Rondinini and Luigi Boitani A wide range of theoretical and applied analyses in animal ecology, biogeography, and ... Foresman has handled a wide diversity of carnivores and teaches a course on making the most of having an animal in hand Mark and Todd Fuller, in Chapter 7, introduce the diversity of telemetry...
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Carnivore Ecology and Conservation: A Handbook of Techniques pptx
Ngày tải lên :
06/03/2014, 18:20
... measures of precision and their meaning 2.4.6 Field methods Mind the map: trips and pitfalls in making and reading maps of carnivore distribution Carlo Rondinini and Luigi Boitani 3.1 Maps based ... map: trips and pitfalls in making and reading maps of carnivore distribution Carlo Rondinini and Luigi Boitani A wide range of theoretical and applied analyses in animal ecology, biogeography, and ... Foresman has handled a wide diversity of carnivores and teaches a course on making the most of having an animal in hand Mark and Todd Fuller, in Chapter 7, introduce the diversity of telemetry...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
Ngày tải lên :
25/10/2012, 11:40
... cellular thiols and maintains the cells integrity [26] Oxidation of proteins can introduce carbonyl groups at some amino acids residues such as lysine, arginine, proline, and threonine in the polypeptide ... addition of 10% TCA and resuspended in 1.0 ml of M HCl for blank and M HCl containing 2% 2,4dinitrophenyl hydrazine After incubation for h at 37°C, protein samples were washed with alcohol and ethyl ... primaquine exposure is noticed These findings are in accordance with pervious study reported that in vitro exposure of RBCs to oxidants resulted in damaging of protein in addition to increase...
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Windows Home Server Protect and Simplify Your Digital Life
Ngày tải lên :
01/01/2014, 16:48
... computers in areas from DOS to Windows Vista, as well as (X)HTML and Web development Working for clients and students — and just out of curiosity — Mark digs deeply into understanding and explaining ... understanding of how Windows Home Server can fit into your home network Chapter 2, “Your Digital Life”: Chapter begins the process of developing a plan for making the most of Windows Home Server in ... with patches, monitoring security settings, and maintaining antivirus software Instead of being an additional burden, Windows Home Server adds integrated network health monitoring to the home users’...
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Tài liệu Physical Activity and Women’s Health pptx
Ngày tải lên :
13/02/2014, 08:20
... gain since age 18 in women has been associated with coronary heart disease (Willett et al., 1995), allcause mortality (Manson et al., 1995), and hyperinsulinemia (fasting insulin and insulin ... comparison of the effects of exercise on insulin sensitivity in women with NIDDM recently revealed that low intensity exercise was as effective as high intensity exercise in enhancing insulin sensitivity ... certain minority women have exceedingly high mortality and prevalence rates from this adult-onset chronic disease Diabetes is a leading cause of death in women, and a leading cause of adult blindness,...
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Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
Ngày tải lên :
14/02/2014, 06:20
... in main family dwellings (households) of Spain and is mainly performed by the Ministry of Health and Consumer Affairs and the National Statistics Institute (Instituto Nacional Estadística-INE) ... the final manuscript Authors’ information None Competing interests The authors declare that they have no financial competing interests and non-financial competing interests Conflict of interest: ... of the study, and participated in its design and coordination and draft the manuscript CAB carried out the acquisition of the data, analysis and interpretation of data She has been involved in...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
Ngày tải lên :
14/02/2014, 19:20
... substrate binding in GH11 xylanases Table The effect of genetic engineering of the secondary binding site of the B subtilis and A niger xylanases on the screening ratio The screening ratio is ... containing (mutant) XAN The results of the screening for XBS and XAN with a modified SBS are shown in Table Results and Discussion XBS Genetic engineering of the SBS Residues of the SBS of both ... binding sites of the A niger xylanase (A) and the B subtilis xylanase (B) The diagrams of protein–ligand interactions were generated using LIGPLOT [37] based on Vandermarliere et al [12] Amino...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
Ngày tải lên :
19/02/2014, 07:20
... total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the 38-kDa protein band was corrected for the amounts of the added proteins with similar sizes The rates of ligation ... conformation of His78 is virtually identical in the single and double mutants involving this residue (Fig 5B,D) In both cases, the side chain is removed from the vicinity of Ser278 and Asp82 and the ... the interactions between Ser278 and Asp164 Thus the introduction of an asparagine instead of an aspartic acid into the oxyanion hole had the unexpected result of shifting the side 2568 chain of...
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Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx
Ngày tải lên :
19/02/2014, 16:20
... Design and synthesis of HIV-1 protease inhibitors Novel tetrahydrofuran P2/P2¢-groups interacting with Asp29/30 of the HIV-1 protease Determination of binding from X-ray crystal structure of inhibitor ... Daly, J.W & Kirk, K.L (2000) Syntheses of (R )and (S)-2- and 6-fluoronorepinephrine and (R)- and (S)-2- and 6-fluoroepinephrine: effect of stereochemistry on fluorine-induced adrenergic selectivities J ... protein inhibitor solution with an equal volume of reservoir solution The protein inhibitor solution contained mgÆmL)1 protein in 50 mM MES, pH 6.5, containing 10 mM dithiothreitol and mM EDTA, and...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
Ngày tải lên :
20/02/2014, 11:20
... transition of serpins in general and latency transition of PAI-1 in particular The data presented provide novel insights into the determinants of serpin stability located in and around hF and support ... hF and b-sheet A (Fig 5) and may be instrumental in promoting the translocation of hF during RCL insertion K325 is also conserved among serpins (K335 in a1PI) [2] and its substitution for A in ... K327 in the coordination of a stabilizing chloride ion [18] or suggest the existence of a stabilizing salt-bridge between the two side chains (Fig 5) In contrast, substituting T96 in s2A, I138 in...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... ng atoms of oxygen consumed per minute and per mg protein The rate of oxygen consumption in state and in state 4, respiratory control ratio (RCR) of oxygen consumptions in states and and the ADP/O ... with aging (Eur J Biochem 270) 2299 Western blotting of modified proteins Fig Determination of CML-protein content in liver mitochondrial matrix with aging The CML content in the matrix proteins ... Overexpression of glyoxalase-I in bovine endothelial cells inhibits intracellular advanced glycation endproduct formation and prevents hyperglycemia-induced increases in macromolecular endocytosis J Clin Invest...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
Ngày tải lên :
20/02/2014, 23:20
... (v/v/v) and sprayed at a flow rate of lLÆmin)1 MALDI-TOF MS of LPS and free lipid A were performed on a Reflex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF configuration applying an acceleration ... control of a partial human E-selectin promoter (pELAM.Tac) which contains an essential nuclear factor, NF-jB binding site CHO cells were grown in Ham’s F12 medium containing 10% fetal bovine serum and ... revealed singly charged molecular ions in the region around m/z 1779, prominent fragment ions of the de-O-acylated lipid A moiety around m/z 1119.75, and ions of minor intensity originating from...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
Ngày tải lên :
07/03/2014, 00:20
... Representatives of this class include certain amino acids (e.g proline and glycine) and polyols (e.g trehalose, sucrose and sorbitol) Counteracting osmolytes consist of the methylamine class of osmolytes, ... l-lysine, l-serine, b-alanine, taurine, sarcosine, dimethylglycine, glycine betaine, trimethylamine N-oxide, and cytidine 2¢-3¢ cyclic monophosphate were also obtained from Sigma These and other ... & Fink AL (2001) Trimethylamine-Noxide-induced folding of a-synuclein FEBS Lett 509, 31–35 Meng F, Park Y & Zhou H (2001) Role of proline, glycerol, and heparin as protein folding aids during...
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Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
Ngày tải lên :
07/03/2014, 00:20
... Fractions of 0.5 mL were collected into Haptoglobin binding to apolipoprotein E tubes containing 10 lL of m Tris, and analysed by electrophoresis Electrophoresis and immunoblotting Electrophoresis in ... o-phenylenediamine as previously described [7] Nonspecific binding was determined by omitting the Hpt loading in the wells Inhibition of Hpt binding to Hb-coated wells, in the presence of ApoA-I or ... lanes and 2, respectively VLDL and LDL proteins, blotted onto the PVDF membrane and incubated with biotinylated Hpt, are shown in lanes and 4, respectively VLDL and LDL proteins, after blotting and...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
Ngày tải lên :
07/03/2014, 00:20
... the interacting subunits In a previous study, the current authors have shown that the mutation of Thr15 to Ala in the vicinity of the hydrophobic core induces a tighter binding of the cofactor in ... Structural symmetry and putative electrostatic interactions within intertwined N-terminal arm Broken lines represent electrostatic interactions in the vicinity of A13 in the homology model of S toebii ... evolutionary engineering of S toebii TPL Open symbols ( ) indicate activity-related mutants and filled symbols ( ) indicate stability mutants obtained during the screening of random mutagenesis...
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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
Ngày tải lên :
07/03/2014, 05:20
... decrease in the frequency of SSS and SSG codons occurs with an increase in Topt, which obscures the uniform increase in G-C content [13] Interestingly, at the level of RNA, there is a growing body of ... that the DNA and RNA of hyperthermophilic microorganisms are also stabilized through a combination of mechanisms, including increased intracellular electrolyte concentrations, binding of positively ... employed in a number of applications, including starch processing, cellulose degradation and ethanol production, pulp bleaching, leather and textile processing, chemical synthesis, food processing, and...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
Ngày tải lên :
07/03/2014, 05:20
... it contains a single ATPase domain incorporating a structure rich in b strands (b domain), an N-terminal microtubule interacting and trafficking (MIT) domain [28–30] and a final C-terminal a helix ... compared binding of an ESCRT-III component, Vps20p, to wildtype and mutant Vps4p proteins in the presence and absence of ATP (Fig 5C) Binding of Vps20p to the Vps4p mutant proteins lacking the ... ability to interact with a panel of known Vps4p interactors indicating that the N-terminal domain is correctly folded in each of the mutant proteins and that the b domain is correctly folded in the...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... replacement of Tyr34 and Trp161 (with phenylalanine in both cases) resulted in a slower rate of zero-order decay (or an increased product inhibition effect) [21,24], certain substitutions of His30 and ... various mutations, and current efforts are focused in this direction The engineering of increased catalytic activity or efficiency in enzymes is a significant challenge in protein engineering, but was ... (Becton-Dickinson, Franklin Lakes, NJ) M63 minimal media, made according to Miller [39], was supplemented with lgÆmL)1 thiamine, as well as 0.5 mm of each of the amino acids l-isoleucine, l-leucine and...
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Báo cáo khoa học: Alpha-fetoprotein antagonizes X-linked inhibitor of apoptosis protein anticaspase activity and disrupts XIAP–caspase interaction ppt
Ngày tải lên :
07/03/2014, 12:20
... presence of the N-terminal conserved BIR-binding motif (IBM), which is required for IAP binding [20] The presence of IBM in the intracellular protein allowed us to recognize its possibility of serving ... al inhibition of IAP function leading to triggering of the apoptosis machinery Further studies are required to better understand the importance of the role AFP in modulating the level of IAPs in ... monitoring of DEVD–AMC cleavage at 5-min intervals Data were collected at 30 °C for 30 and expressed in RFU The mean ± SD of three independent determinations is shown incubated with rhXIAP and AFP...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Ngày tải lên :
07/03/2014, 15:20
... °C min/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C min/94 °C; min/54 °C; min/72 °C min/94 °C; min/57 °C; min/72 °C min/94 °C; min/54 °C; min/72 ... ligand-free protein, the authors docked several GCPII inhibitors into the GCPII-binding pocket and proposed/ analyzed the amino acid residues involved in the ligand– protein interactions All of the residues ... region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and 587–750 (domain F) While the domain spanning amino acids...
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