treatment preparation of cell lysates and immunoblot
Control of Cell Proliferation and Growth byMyc Proteins
... deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the ... in cell proliferation: for example, deletion of n-myc in neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of ... generally required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins...
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preparation of wo3 nanoparticles and application to no2 sensor
... 3, and 10 kPa and annealed at 600 8C (b) WO3 nanoparticles deposited at kPa and annealed at 400 and 600 8C results for the sensors made of WO3 nanoparticles deposited at 1, 3, and 10 kPa and ... FE-SEM images of WO3 nanoparticles deposited at 1, 3, and 10 kPa, respectively, and annealed at 600 8C (d)–(f) FE-SEM images of WO3 nanoparticles deposited at kPa and annealed at 400, 600, and 800 ... size of 36 nm deposited at kPa and annealed at 400 8C and that for the sensor made of large particles with an average particle size of Please cite this article in press as: D Meng, et al., Preparation...
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Báo cáo hóa học: " The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction" pptx
... for 10 and immediately vortexed prior to addition to cell cultures Isolation of Peripheral Blood Mononuclear Cells For isolation of PBMC (peripheral blood mononuclear cells) and immune cell subsets, ... (FBS) and cultured at 37° C and 5% CO2 Isolation and Culture of Primary CD14? Monocytes and CD4? T cells PBMC were obtained by Ficoll-Hypaque density centrifugation as described above, and CD14? cells ... including T cells, B cells, natural killer (NK) cells, and monocytes For these studies, freshly isolated peripheral blood mononuclear cell (PBMC) preparations were used, since all of the desired cell...
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Báo cáo khoa học: "Malignant mixed Mullerian tumors of the uterus: histopathological evaluation of cell cycle and apoptotic regulatory proteins" ppt
... [24] demands the attention and time of researchers in a fight to improve treatment modalities and widen the understanding of uterine MMMT Conclusions Our study supports that cell cycle and apoptotic ... during cell cycle proliferation Proliferative markers such as Ki67 provide an index of cells in the Go/G1 pool of cycling cells [30] Higher fractions of these cells represent an increased number of ... is one aspect of mammalian cell behavior, which is of central importance in growth and development and plays a key role in tumor-oncogenesis The three key features of apoptosis and cell survival...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... concentration of 10e5 cells/well in a 24 well plate After 24 hours, cells were infected with ng of p24 antigen of different HIV strains, incubated at 37°C with 5% CO2 for hours, washed thoroughly and cultured ... Larger studies and data sets are needed from these regions to further substantiate these findings and to understand the complexity of the emerging diversity of HIV in this region of high diversity ... are capable of using both co-receptors and are termed dual tropic or R5X4 viruses In the era of antiretroviral therapeutics, co-receptor antagonists are now in use for treatment of HIV infected...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... inhibited cell viability by 5%, and Raft-media was cytotoxic to 18% of cells (Fig 2b) Raft-media has many antibiotics, which, when combined, result in cytotoxicity Inhibition of cell- free and cell- associated ... of PVP-coated AgNPs (0.3 and 0.6 mg/mL) were only cytotoxic to a small population of cells, affecting the viability of 20-23% of the cells in the cervical explant, which correlates with low off-target ... Institute of Allergies and Infectious Diseases and the National Institute of Health and Collaborators: MT-2 (from Dr Douglas Richman), H9+ cells (from Dr Robert Gallo), HeLa-CD4-LTR-β-gal cells and...
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báo cáo khoa học: "Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin" pot
... composition and structure of the cell wall that governs the cell expansion direction and rate As in land plants, the primary cell wall of M denticulata Bréb consists mainly of pectins [10,11], cellulose ... evolution of genes involved in cell wall formation in green algae and land plants Cell walls have played crucial roles in the colonization of land by plants [63,64] For a detailed understanding of how ... cell wall components appear to be adaptations of land plants, cell wall evolution after the colonization of land is seemingly characterized by the elaboration of a pre-existing set of genes and...
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báo cáo khoa học: " Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction" ppt
... wild-type cells To examine cell division in the roots of intact plants, the percentage of cells in the three phases of the cell cycle–G1, S, and G2/M–were evaluated with the Partec software The ... the three phases of the cell cycle–G1, S, and G2/M–were evaluated by the Partec software When cell division indices (ratio of G2M% vs G1%) were compared, the portion of wild-type cells in G1 phase ... stimulate cell division [43-46], which was based on observations of the effects of BRs on cultures of suspension cells or protoplasts At the molecular level, it was found that the stimulation of cell...
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Báo cáo y học: "Routine versus needs-based MRI in patients with prolonged low back pain: a comparison of duration of treatment, number of clinical contacts and referrals to surgery" pdf
... retrieve information on, and compare the duration of, treatment, number of contacts with clinicians, and referral for surgery that occurred before and after the practice of routine MRI However, ... of back pain and leg pain, and functional disability (Table 1) The median age for both groups was 48 years and there was almost an even distribution of men and women The median for leg pain and ... setting, depending on the type of governmental payment structure, type of treatment and structure of treatment program Our results are based on a crude calculation of the direct costs, which were...
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Báo cáo y học: "RNA interference pinpoints regulators of cell size and the cell cycle" doc
... phases of the cell cycle as well as cell size The simultaneous effect of each dsRNA on six different cellular phenotypes was recorded: the percentage of cells with a DNA content of 2N (percentage of ... (percentage of cells in G1; 2N denotes cells in G1); 4N cells (percentage of cells in G2, the phase after the DNA has been replicated); less than 2N (percentage of dying cells); and greater than ... decreased the percentage of cells in G2, increased the percentage of cells undergoing apoptosis and decreased both G1 and G2 cell size It is tempting to speculate that these cells are impaired in...
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Modeling and simulation of cell adhesion and detachment
... Structures and Functions of Cell As the basic unit of life, the cell is a biologically complex system [49] There are two types of cells: prokaryotic and eukaryotic Prokaryotic cells, such as archaea and ... studies of neutrophil adhesion [35-39], examination of cell membrane’s thickness and compressibility [40], and inspection of cell- surface interactions [41] A major focus of designing and studying ... MODELING AND SIMULATION OF CELL ADHESION AND DETACHMENT SUN LU (B Eng., FDU China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MATERIALS SCIENCE AND ENGINEERING...
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Preparation of black rice and blueberry vinegar beverage by multi strain immobilized fermentation
... Gel strength of the native egg white[J] Czech Journal of Food Science, 2004,22:58-66 [9] Chin K B, Go M Y, Xiong Y Konjac flour improved textural and water retention properties of transglutaminasemediated, ... retention properties of transglutaminasemediated, heat-induced porcine myofibrillar protein gel: Effect of salt level and transglutaminase incubation[J] Meat Science,2009,81(3):565-572 [10] 朱玉安,刘友明,张秋亮,等.加热方式对鱼糜凝胶特性的 ... mL，还原糖含量≥1.00 g/100 mL其 他致病菌未检出。黑米蓝莓果醋饮料感官指标， 即色泽为蓝色，澄清透明有光泽，允许瓶底略有 · 247 · 添加剂与调味品 食品科技 FOOD SCIENCE AND TECHNOLOGY 积聚物存在；酸甜适口，具有特有的黑米酿造香 气无其他异味。 讨论 通过试验证实固定化乳酸菌发酵蓝莓果醋饮 料是一种较为理想的方法。 4.1 最佳固定化乳酸菌的方法...
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Báo cáo y học: "Short- and long-term effects of anti-CD20 treatment on B cell ontogeny in bone marrow of patients with rheumatoid arthritis" ppsx
... Teng and colleagues [33] showed that 88% of RA patients had a reduction of B cells in synovium four weeks after treatment and that clinical responders had less infiltration of CD20+ and CD138+ cells ... t-test B cells), and IgD-CD27+ (switched memory B cells and plasma blasts/cells) [40,41] The maturation level of the B cell populations was determined using a combination of CD38, CD24, and IgD: ... Accumulation of immature subset of B cells in BM long after rituximab treatment We found a proportional increase of immature and transitional (CD38++IgD-, P = 0.002) and a reduction of Bm5 cells (CD38+IgD-,...
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TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS
... cell types Nonetheless, limiting the expansion of cell death and tissue damage are primary goals for acute treatment following SCI and TBI and other CNS injuries Part of the contribution to cell ... of inflammatory and foreign molecules and cells into the typically secluded parenchyma of the CNS (Tator and Fehlings, 1991, Casella et al., 2006) Currently, the involvement and interaction of ... thorough overview of pathology and cell- and signal-specific mechanisms of survival and repair in a clinically relevant rodent SCI model Xiao-Ming Xu, Ph.D., Chair v TABLE OF CONTENTS List of Tables...
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Báo cáo y học: "Effect of Acute Administration of an Herbal Preparation on Blood Pressure and Heart Rate in Humans"
... decongestants and is not a constituent of C aurantium Because of the known cardiovascular effects of nor-epinephrine and ephedrine, it has generally been assumed that consumption of p-synephrine and bitter ... and increase metabolism, suppress appetite, and/ or slow the absorption of fats and carbohydrates [5] In spite of their widespread use and the lack of credible reports regarding adverse effects, ... blood pressure and heart rate, regardless of treatment or subject grouping either by gender or caffeine usage Subjects in the present study ingested 39 mg of p-synephrine and 528 mg of caffeine...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... these concentrations of matrine and RGD-LCL After 24 h of 200 treatment, the cells were collected, washed with cold phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V ... successful coupling of the RGD to the surface of the liposomes Assay of particle sizes and morphological observation of RGD-M-LCL The average size of liposomes processed by the microfluidizer was ... the anti-proliferative and pro-apoptotic effects of matrine in cancer cells RGD-M-LCL significantly inhibited the growth of Bcap-37, HT-29 and A375 cells, compared with cells treated with the...
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Báo cáo y học: "Comparison of Classical and Clozapine Treatment on Schizophrenia Using Positive and Negative Syndrome Scale of Schizophrenia (PANSS) and SPECT Imaging"
... in winter and early spring, and viral infections in the mother during the second and third trimesters of gestation A strong association between hypofrontality and negative symptoms of schizophrenia, ... passivity and apathy, difficulty in abstract thinking, lack of spontaneity and flow of conversation, and stereotyped thinking (N1 to N7); and health concerns, anxiety, guilt, tension, mannerisms and ... MRI and CT, creating a single image that combines anatomy and physiology Three dimensional surface and volume- rendered images add perspective and facilitate the localization and sizing of lesions...
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Occurrence of Tetracycline-Resistant and Tetracycline- Degrading Bacteria in Wastewater Treatment Plant Effluent and Environmental Water Systems
... Characteristics of Pharmaceuticals in Wastewater Treatment, - 326 - Journal of Water and Environment Technology, Vol 8, No.4, 2010 J of Water and Waste, 50(7), 587-593 Wen X., Jia Y., and Li J., (2009) ... Tahara M., Shimizu K and Nisimura T (2008) Monitoring of PPCPs in Aquatic Environments in Japan and Evaluation of Removal Efficiency on Assumption of Water Purification Process, J of Japan Society ... Kunikane S (2008) Occurrence and Removal of Pharmaceuticals in Drinking Water Treatment Plants, J of Water and Waste, 50(7), 581-586 Simpson J M., Santo Domingo J W and Reasoner D J (2002) Microbial...
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A CFD analysis of transport phenomena and electrochemical reactions in a tubular-shaped PEM fuel cell
... were independent of the grid size A computational quadratic mesh consisting of a total of 11371 nodes and 60778 meshes for planar cell and 16927 nodes and 94547 meshes for tubular cell were found ... onset of pore plugging, which has a detrimental effect of the fuel cell performance This model is used to analyse and evaluate the performance of a planar and a tubular-shaped PEM fuel cell 2.1 ... distribution of reactant species in this design of the perforated type gas distributor One of the new architectures in PEM fuel cell design is a tubular-shaped fuel cell Fuel cell companies and research...
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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx
... have focused upon the role of miRNAs in regulatory network of various cell signaling pathways We summarize the current knowledge of the interdependence of miRNA and cell signaling pathways, which ... of E2F and Myc [52] In tumor progression, the transcription repressors ZEB1 and SIP1 and the miR-200 family of miRNAs provide a double-negative-feedback loop that regulates the phenotype of cells ... tumors and cell lines, miR-175p and miR-20a are induced in a manner that depends on cyclin D1 and repress the expression of cyclin D1 Hence, miR-17-5p ⁄ 20a and cyclin D1 form a feedback loop and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose