transcription pre mrna splicing and dna damage
Báo cáo y học: "The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing" doc
Ngày tải lên :
14/08/2014, 14:21
... that some splicing- related genes might regulate the splicing of other splicing- related genes For example, overexpression of AtGRP7 and atSRp30 is known to affect the splicing of AtGRP8 and atSR1, ... snRNAs) and high alternative splicing frequency in U1 snRNP proteins, SR proteins and hnRNP proteins The SR proteins and U1 snRNP proteins are involved in early steps of splicing and 5' and 3' ... unrelated to splicing were removed from the main candidate list, and all candidates with significant similarity to proteins related to splicing were regarded as true splicing- related genes and were...
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Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot
Ngày tải lên :
21/02/2014, 15:20
... gene expression, transcription initiation and pre- mRNA splicing MATERIALS AND METHODS Plasmid and oligonucleotides The pre- mRNA b-globin, derived from the second b-globin intron of rabbit pre- mRNA ... gel and eluted from the gel after UV-shadowing In vitro splicing assays Standard splicing of the b-globin pre- mRNA in HeLa nuclear extract was performed at 30 °C for 60 Splicing reactions were prepared ... nuclear pre- mRNA splicing, as indicated by a large reduction in excised lariat RNA and a corresponding amount of unchanged pre- mRNA, respectively (lanes 2, and 5) The three inhibitory antibiotics and...
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Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
Ngày tải lên :
16/03/2014, 02:20
... Ki-1 ⁄ 57 and pre- mRNA splicing G C Bressan et al A D B E C F G H Fig Confirmation of the protein–protein interactions among Ki-1 ⁄ 57 and proteins involved in pre- mRNA splicing (A–C) Immunoprecipitation ... well known that in eukaryotes, transcription and pre- mRNA maturation events (5¢-capping, splicing, 3¢-end processing and polyadenylation) occur cotranscriptionally and that the machineries responsible ... influences the splicing pattern of the E1A pre- mRNA (A) Diagram showing the splicing events that generate the 13S, 12S, 10S and 9S mRNAS of the E1A reporter gene [22,23] (B–D, G) In vivo splicing assays...
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Báo cáo y học: "Intronic motif pairs cooperate across exons to promote pre-mRNA splicing" docx
Ngày tải lên :
09/08/2014, 20:22
... enriched among genes preferentially expressed in prefrontal Page 11 of 18 cortex, thyroid and many immune tissues (BDCA4 + dendritic cells, CD14+ monocytes, CD4+ T cells, and so on), and significantly ... Mechanisms of fidelity in pre- mRNA splicing Curr Opin Cell Biol 2000, 12:340-345 Carothers AM, Urlaub G, Grunberger D, Chasin LA: Splicing mutants and their second-site suppressors at the dihydrofolate ... index (see Materials and methods) described by Segre et al [44] and Elena and Lenski [45] (Figure 9e) We also tested two ‘neutral’ non-co-occurring pairs (N1 and N2) as tandem pentamers These...
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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE
Ngày tải lên :
09/09/2015, 08:16
... of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage increases following ... cytotoxicity and mutagenesis, respectively [21, 22] Here, we hypothesize that influenza infection induces DNA damage, and that DNA damage responses modulate cytotoxicity and tissue damage in infected ... is known that the predominant forms of DNA damage generated by endogenous stresses are single strand lesions, such as base damage, abasic sites and SSBs [39, 40] While DNA strand breaks can arise...
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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE
Ngày tải lên :
30/09/2015, 06:20
... bases, deaminated bases, and DNA- DNA and DNA- protein cross-links (Lonkar and Dedon 2011) In addition, attack of ROS / RNS on DNA backbone can also lead to DNA single strand breaks (SSBs), which ... cytotoxicity and ultimately disease DNA damage response (DDR) pathway is a complex signaling network that has evolved to sense for DNA damages, activate cell signaling and repair DNA lesions DNA damage ... ONOO- and OH• will overwhelm antioxidant systems and exacerbate DNA damage OH• radical is considered one of the most potent ROS that leads to DNA damage by oxidizing bases and attacking DNA backbones...
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Checkpoint and Coordinated Cellular Responses to DNA Damage
Ngày tải lên :
25/10/2013, 21:20
... ATR-ATRIP, Rad17, and 9-1-1 complexes can independently recognize damage- induced DNA structures such as ssDNA and junctions of dsDNA/ssDNA Moreover, RPA appears to play important roles in the damage recognition ... of DNA damage is coupled to a specific DNA repair pathway as well as the cell cycle regulatory apparatus Certain types of Checkpoint and Coordinated Cellular Responses to DNA Damage 73 DNA damage ... interaction between ATR-ATRIP and RPA-ssDNA, and might contribute to the activation of ATR Transduction of DNA Damage Signals After ATM and ATR are activated by DNA damage, they phosphorylate numerous...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
Ngày tải lên :
18/02/2014, 16:20
... causes DNA damage in hepatocytes Transplant Proc 26, 907–909 Moller P, Loft S, Lundby C & Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage ... As both hypoxia and reoxygenation have been reported to induce DNA damage [30–32], we examined the effects of pyruvate addition during hypoxia and after reoxygenation on DNA damage HepG2 cells ... single-strand and double-strand breaks Pyruvate reduces DNA damage during ⁄ after hypoxia Cells cultivated in Petri dishes (25 mm in diameter) were suspended in 0.5 mL of NaCl ⁄ Pi, and cell density...
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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
Ngày tải lên :
21/02/2014, 01:21
... intensity was 45% of its initial abundance DNA strand break analysis The ability of Cu(II)-Ami to induce strand breaks in plasmid DNA in the absence and presence of H2O2 was tested with the use ... site-specific DNA damage induced by copper (II) ion and hydrogen peroxide J Biol Chem 264, 15435–15440 37 Oikawa, S & Kawanishi, S (1996) Site-specific DNA damage induced by NADH in the presence of ... region, at m/z of 1232 and 1294 They can be assigned to single-charged molecules of Cu(II)Ami2 and Cu(II)2-Ami2, respectively Also, two additional Ó FEBS 2002 DNA and RNA damage by Cu(II) amikacin...
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Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
Ngày tải lên :
07/03/2014, 10:20
... distribution of WISP1 and its physiological roles in normal tissues In this study, we analyzed the expression of fulllength WISP1 mRNA and its splicing variants in normal and malignant-transformed ... cancer and MDA231 breast cancer cells Vascular endothelial cells (HUVEC) and A371 melanoma cells expressed only full-length mRNA (Fig 1B), whereas osteoblastic SaOS2 expressed both CCN4 ⁄ WISP1 and ... amplicons I, II and III from HCS-2 ⁄ cDNA were analyzed and the structures of the corresponding mRNAs and deduced translational products are illustrated Owing to the alternative splicing, a frameshift...
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Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt
Ngày tải lên :
16/03/2014, 00:20
... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated ... cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein kinase (HIPK2) Multiple functions of p53 in DNA damage- induced apoptosis The tumour suppressor and transcription ... cellular context and the extent of DNA damage – which determines whether or not damage is reparable – the activated DDR can trigger different cellular responses Mild DNA damage is usually handled through...
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Báo cáo khoa học: Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms docx
Ngày tải lên :
30/03/2014, 11:20
... other hand, m3 bands were observed at 1471 and 1493 cm)1, representing the five-coordinate high-spin and six-coordinate low-spin states, respectively Because of the sensitivity of the Fe–CO and C–O ... cry, whereas it represses BMAL1 gene expression Like CLOCK and BMAL1, NPAS2 has bHLH, PAS-A and PAS-B domains in its N-terminal region, but in contrast to CLOCK, both the PAS-A and PAS-B domains ... the E-box of DNA using the quartz-crystal microbalance (QCM) method Results Protein expression and purification We attempted to express the isolated bHLH-PAS-A (amino acids 1–240) and PAS-A (amino...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... Antonio Migheli Introduction 1.1 DNA Damage, Apoptosis, and Necrosis A number of exogenous and endogenous toxic agents may damage DNA, leading to genomic instability and transcriptional infidelity ... of DNA Damage: Methods and Protocols Edited by: V V Didenko © Humana Press Inc., Totowa, NJ Walker et al Fig Patterns of DNA fragmentation in apoptosis (A) DNA extracted from control (lane 1) and ... single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA This is particularly useful since many single-strand breaks are also introduced into DNA...
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báo cáo hóa học:" Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)" pptx
Ngày tải lên :
20/06/2014, 07:20
... ERCC1/XPF and XPG) are recruited to the DNA damage recognition complexes in both TCR and GGR to remove the damage in a common pathway Over-expression of XPA and ERCC1 mRNA has been associated with cisplatin ... bulky DNA damage that is repaired mostly by the nucleotide excision repair system (NER) Cellular response to cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair ... determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum -DNA damage recognition protein in global genome repair (GGR) [34] subpathway of NER, and of ERCC1 and XPA, downstream...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
Ngày tải lên :
28/06/2014, 05:20
... 6 DNA Repair − On the Pathways to Fixing DNA Damage and Errors 4.3 Dna2 as a preemptive means to prevent formation of long flaps 4.3.1 Long flaps are in vivo substrates preferred by Dna2 Dna2 ... domain of Dna2 and Mph1 Since overexpression of Mph1 also suppressed the dna2 405N mutant, the suppression of the Dna2 defect by Mph1 is due to the stimulation of Fen1 activity, and not of Dna2 Rad52 ... ts phenotype of dna2 405N, but failed to suppress the double mutants, dna2 405N rnh201, dna2 405N rnh202 and dna2 405N rnh203Nguyen et al., 2011 Thus, the suppression of dna2 mutation also...
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Báo cáo y học: "Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 01:23
... tive damage to DNA is excessive, repair is not possible and apoptotic mechanisms are induced Although both nDNA and mtDNA undergo oxidative damage [4], it is intriguing to note, first, that mtDNA ... artefacts These precautions included the use of sterile equipment and dedicated pre- PCR and post-PCR rooms, pipettes, laboratory coats, disposable gloves and waste containers To prevent any contamination ... with RA and occurrence of mtDNA and oxidized DNA The laboratory findings for the patients with RA used in this study are shown in Tables and There was no significant correlation between the presence...
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Báo cáo khoa học: "Radiation induced apoptosis and initial DNA damage are inversely related in locally advanced breast cancer patients" ppt
Ngày tải lên :
09/08/2014, 09:20
... between initial DNA damage, measured as DSB, and RIA Ionizing radiation (IR) kills cells by damaging the structure and function of genomic DNA The response of cells to this damage and their ability ... Ruiz de Almodovar JM: DNA damage and prediction of radiation response in lymphocytes and epidermal skin human cells Int J Cancer 1998, 76:354-361 Saavedra MM, Henriquez-Hernandez LA, Lara PC, Pinar ... above background and observations, the aim of the present study was to analyze if there was any statistical relation between initial DNA damage, estimated by the number of DSB, and the apoptotic...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
Ngày tải lên :
09/08/2014, 09:20
... phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines Cancer Res 2004, 64(19):7144-7149 47 Bilsland E, Downs JA: Tails of histones in DNA double-strand break ... prior to irradiation and were stained at 24 and 48 h postirradiation (2 Gy) Both adherent and detached cells were collected, centrifuged, and double stained with Annexin V-FITC and propidium iodide ... 24 h Data displayed by the DNA content profiles were analyzed, and the cell cycle phase information is represented graphically markers for evaluating the level of DNA damage [24] To investigate...
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Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
Ngày tải lên :
09/08/2014, 09:20
... initial DNA damage and RIA in LABC patients [23] Taking into account the above background and our previously observations, we explored the clinical association between initial DNA damage and RIA ... possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new data which may help to understand and define the complex ... experiments and data acquisition MIN has been involved in conception and design of the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of...
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Transcription-coupled mutagenesis by the DNA deaminase AID Erik D Larson and Nancy Maizels ppt
Ngày tải lên :
09/08/2014, 20:20
... and Alt laboratories [11,12] showed that, while AID does not attack duplex DNA, it will deaminate cytidines in single-stranded DNA within the exposed single-stranded region of an artificial transcription ... recombination and somatic hypermutation on transcription, and for the correlation between the level of hypermutation and the level of transcription Moreover, AID was shown to be processive in vitro and ... with the transcription apparatus (tailed arrows) at the expressed variable region and the activated S and S␥1 switch regions The shaded box illustrates how AID first deaminates C to U, and then...
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