titration of potassium permanganate vs oxalic acid
ứng dụng chất kích kháng CuCl2 và Oxalic Acid
... 2.7: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các thời điểm 40, 50 và 60 NSKS 42 Hình 2.8: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2.13: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các thời điểm 40, 50 và 60 NSKS 50 Hình 2.14: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2001) và mô học (Huỳnh Minh Châu và ctv,2001). 6. Đặc tính oxalic acid và tác động của nó đối với cây trồng Tên hóa học: OXALIC ACID Công thửc hóa học: Thường tồn tại ở dạng ngm 2 phõn t...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
... potential role of cAMP inmediating the effects of CT on endosome acidification[13]; and (c) the effects of CT were not observed whenendosome acidification was measured in the absence of ATP, possibly ... action of cholera toxin under the con-trol of endosomal acidity was confirmed using cellular systems in whichmodification of the expression levels of cathepsin D, either by transfection of the ... endosomal acidifi-cation at the early stage of CT action.Role of endosomal acidification and cathepsin Din CT actionTo assess whether the aspartic acid protease cathep-sin D and endosomal acidity...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... Murakami2and Kenji Aoki21Division of Science of Biological Resources, Graduate School of Science and Technology,2Department of Biofunctional Chemistry,Faculty of Agriculture, Kobe University, ... (A) The reaction mixtureconsisted of 2.9 mL of 100 mMsodium /potassium phosphate buffer(pH 7.5), 0.1 mL of 5 mM4-amino-3-hydroxybenzoic acid, and0.05 mL of the crude extract (3575% ammonia ... 3-hydroxyanth-ralinic acid differs from that in the pathway for 4-amino-3-hydroxybenzoic acid. The N-terminal amino acid sequence of the purifiedenzyme d id not show significant levels of identity tosequences of...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... therole of key acidic amino acid residues in staphylococcalnuclease unfolding. FEBS Journal 272, 3967–3974.7 Chen HM, Markin VS & Tsong TY (1992) pH inducedfolding ⁄ unfolding of staphylococcal ... the sum of fractional multiples of the reference spectra for each structural type.Figure 1A shows the CD spectra of W140A, E142Oand K133A. There are no significant differences fromthat of the ... thesecondary structure of the protein disintegrated. Usingthe peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... similar to the group B mutations of EF-G,because, in the FA complex of EF-G with the ribo-some, the C-terminus of ribosomal protein L6 is indirect contact with domain V of EF-G [22].Group C mutations ... and GTP forms of EF-Tu. (C) Fo)Fcomit map of the switch IIregion of S. aureus EF-G contoured at 3r. Omitted residues are shown in yellow stick representation. (D) Comparison of the switch ... structure of S. aureus EF-G to 1.9 A˚resolution, allowing us to examine the generality of conclusions drawn from the T. thermophilus EF-Gstructures and to pinpoint the role of amino acidsthat...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
... NetherlandsBenzoic acid is important for the food industries.Along with other weak acids such as sulfite and sulfurdioxide, sorbic acid, acetic acid, propionic acid andlactic acid, benzoic acid is used ... 75% of their concentration in the absence of benzoic acid (Table 3).One striking difference between the two steady-statesis that the concentrations of the weak acids in thetricarboxylic acid ... absence of benzoate. Equation 7 shows that theexport of 1 mol of benzoate leads to an extra O2con-sumption of 1 ⁄ (P ⁄ O) = 0.68 mol. If the exporter wereto export benzoic acid instead of the...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... Germany). One unit(U) of tetrathionate hydrolase was defined as the amount of protein required for the hydrolysis of 1 lmol of S4O62–in1min.Other enzyme assays Acid phosphatase activity ... measuredspectrophotometrically at 405 nm. One unit of the acid phosphatase activity was defined as the amount of proteinwhich catalysed the formation of 1 lmol of p-nitrophenol in1 min. Glc6P dehydrogenase ... enzyme. The production of NADPH was determinedspectrophotometrically at 340 nm. One unit of Glc6Pdehydrogenase was defined as the amount of protein thatreduced 1 lmol of NADP+in 1 min. Succinate...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... Kmand Vmax of various amino acids as a function of the membrane potential in PAT2 expressing oocytes. Membranepotential dependency of the apparent Km(A) and Vmax(B) values of b-alanine ... Uptake of L-[3H]proline (100 lM) was measured at pH 6.5 for 10 min in the presence of unlabeled amino acidsand derivatives at a fixed concentration of 10 mMandaregivenaspercentageinhibitionofthecontroluptakemeasuredintheabsenceofinhibiton.Data ... specificity of mPAT2. (A,B) Representative current traces of oocytes expressing mP AT2 in response to perfusion with 20 mM of the different substrates in Na+-free buffer at pH 6.5. (C,D) Inhib ition of...
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Báo cáo khoa học: Alpha-oxidation of 3-methyl-substituted fatty acids and its thiamine dependence pptx
... Properties of the enzymatic steps/enzymes of the a-oxidation pathway. The table gives an overview of the present knowledge of some of theproperties of the enzymes involved in the initial degradation of ... P.P.(1996) a-Oxidation of 3-methyl-substituted fatty acids in rat liver.Production of formic acid instead of CO2, cofactor requirements,subcellular localization and formation of a 2-hydroxy-3-methyl-acyl-CoA ... correlationbetween the level of phytanic acid accumulation and theseverity of the clinical symptoms. Interestingly, 30–40% of the patients are born with an absence of one of themetacarpals or metatarsals...
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Competitive sorption of cadmium and lead in acid soils of central spain
... similar proportions of phyllosilicates in the V 2mm soil fraction and of goethite and haematite in theclay fraction. The greater content of illite and themoderate quantity of smectite provide ... we designed an investigation to exam-ine the effect of the simultaneous presence of Pb andCd on sorption behavior to acidic A horizons of tem perate soils from Spain. Specifically, we haveundertaken ... ionic strength of the solution as calculated by Eq. (1).S. Serrano et al. / Geoderma xx (2004) xxx–xxx4 ARTICLE IN PRESSCompetitive sorption of cadmium and lead in acid soils of Central SpainS....
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Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx
... .Fatty acid methyl esters of total lipids from chloroformextracts of yeast cells were prepared by alkaline transme-thylation using 2 mL of 0.1Msodium methoxide for 5 minat 90 °C. Preparation of ... fatty acidsinto their lipids. In absence of exogenous fatty acids, theyeast expressing the C. alpina acetylenase gene converted upto 0.65% of their fatty acids into 1 8:2 in a ratio of D12(E)to ... ontrol of a truncated version(FP1) of the seed-specific napin promoter [10]. Transfor-mation and cultivation of the A. thaliana plants was asdescribed earlier [11]. All fatty acid analyses of thetransformants...
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Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot
... nonspe-cific and is capable of utilizing a variety of 2-ketoacids. The active site of the enzyme contains eight conserved polar amino acids and we havemutated each of these in order to dissect ... Purification andproperties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium. J Biol Chem 244, 1118–1127.R. Tyagi et al. Mutagenesis of the active site of E. coli KARIFEBS Journal ... apparentKdfor [Mg2+] of 2.06 ± 0.38 mM.R. Tyagi et al. Mutagenesis of the active site of E. coli KARIFEBS Journal 272 (2005) 593602 ê 2005 FEBS 597 of 8.0). When each of these residues is...
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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
... polyadenylation site.The cDNA sequence contained an ORF of 414 bpcoding for a polypeptide of 137 amino acids. Thededuced amino acid sequence of the zebrafish CRAB-PIb showed highest identity to ... distribution of thebrain-type fatty acid- binding protein (fabp7a) mRNAis localized to the pariventricular grey zone of the optictectum of the adult zebrafish brain [24]. Similar pat-terns of mRNA ... levels of crabp1b transcripts may be below the level of sensi-tivity of detection by the technique of tissue sectionin situ hybridization.DiscussionThe differentiation and development of primary...
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Báo cáo khoa học: Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum pot
... intensity of staining was probed as afunction of 10–100 lg of labeled polymalate. At lowconcentration, the success of staining depended on the time of injection in the cell cycle. The majority of ... while the amount of the probe and the time of administration relative to thecell cycle were varied. After the administration of smallamounts of the probe, high numbers (>90%) of nuclei werestained ... intensity was independent of the time of injection.Staining by the overlay method as in Fig. 1 or bymicroinjection of 10 lg or less of the conjugate resulted inthe same type of fluorescent nuclei...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
... contribution of residue Y238 toTable 1. Semiquinone formation and stabilization, and redox potentials of the free forms of wild-type and Y238 mutants of D-amino acid oxidase. Thesemiquinone form of ... obtained by subtraction of the absorbance spectrum of thefree oxidized form of DAAOs to the spectrum of the same enzymeafter addition of a saturating concentration (% 20 mM)ofsodiumanthranilate.Table ... imino pyruvate.Fig. 5. (A) Spectral courses of anaerobic reduction of Y238F DAAO byD-alanine and (B) plot of the dependence of the observed first rate of anaerobicreduction (kobs1) for wild-type...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25