titration of an oxalic acid solution with standard potassium permanganate
Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx
... Interpretation of distances between Asp171 and a virtual proximal oxygen of H2O2in thenative and D171N-CN. The native and D171N-CN structures are superimposed on the heme planes. Note that Asn171 of ... pocket.DiscussionSwinging of Asp171It is important to note that OD2 of Asp171 in native-CN and ND2 of Asn171 in D171N-CN formed hydro-gen bonds with different molecules. As a result, thepositions of OD2 and ND2 ... Tokyo Institute of Technology, Japan2 Department of Bioresources and Environmental Sciences, Kyoto Sangyo University, JapanIntroductionHeme peroxidase, one of the best-studied and mostubiquitous...
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Báo cáo khoa học: On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity docx
... the W50P variant hasABCFig. 4. Oxidative half-reaction of wild-type and variants of DAAO.(A) Comparison of time courses of the (re)oxidation of reducedwild-type and W50 variants of DAAO followed ... study represents an extension of theseprevious studies with the goal of enhancing thereactivity of DAAO with dioxygen. It is based on thepredictions of the implicit ligand sampling analysis[16], ... chromatography and spectral analyses,and in the oxidized state show the typical spectrum of FAD-containing flavoproteins (i.e. absorbance maximaat 455 and 375 nm, an e455 nm of 12 600 m)1ặcm)1,and...
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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... corresponds tothe transmembrane region of APP, and has the typicalamino acid composition of transmembrane helices, i.e. small(Gly and Ala) and hydrophobic (Ile, Leu, Met and Val)residues [36]. ... quartz cell of 1.0-mm pathlength. Spectra were collected over the wavelength range260–190 nm with a bandwidth of 2.0 nm and a timeconstant of 8.0 s, and corrected for the contribution of thebuffer. ... (149sequential and 134 medium range) constraints, which wereused to generate an ensemble of 100 structures by the standard protocol of simulated annealing in torsion anglespace implemented in DYANA (using...
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Báo cáo y học: "Efficacy of Sirolimus-Eluting Stents Compared With Paclitaxel-Eluting Stents in an Unselected Population With Coronary Artery Disease: 24-Month Outcomes of Patients in a Prospective Non-randomized Registry in Southern Turkey&
... the small number of patients, lack of direct randomiza-tion, and relatively low compliance with angiographic follow-up. CONCLUSIONS On the basis of the clinical results of this 24-month ... Turkey Davran ầiỗek1, Hasan Pekdemir2, Nihat Kalay3 , Süleyman Binici4, Hakan Altay4, Haldun Müderrisoğlu5 1. Başkent University School of Medicine, Department of Cardiology, Antalya, ... Department of Cardiology, Adana, Turkey; 5. Başkent University School of Medicine, Department of Cardiology, Ankara, Turkey Corresponding author: Davran Cicek, Başkent University School of Medicine,...
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Báo cáo y học: " Differentiation of convulsive syncope from epilepsy with an implantable loop recorder Khalil Kanjwal, Beverly Karabin, Yousuf Kanjwal, Blair P Grubb"
... Syncope, the transient loss of consciousness with spontaneous recovery occurs as consequence of a pe-riod of cerebral hypoxia. A number of conditions may disturb cerebral oxygenation, ranging from ... 41-year-old woman was referred for evalua-tion of recurrent convulsive episodes. At the age of 29 years, she began to experience episodes of sudden loss of consciousness associated with convulsive ... (14) found that close to 45% of patients with atypical seizures had a cardiac related cause of these episodes, and it was only because of prolonged monitoring with an ILR that allowed this identification...
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Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc
... well-definedhelix, with F and Y torsion angle pairs fallingwithin the allowed a-helical region of the Ramachan-dran plot [41]. Under aqueous conditions, deviationsfrom the allowed regions of the Ramachandran ... one of themost abundant proteins in CNS myelin; MBP maintainsthe compaction of the sheath by anchoring the cytoplas-mic faces of the oligodendrocyte membranes [2], and is acandidate antigen ... the Canadian Institutes of Health Research (Operating Grant MOP 43982 toGH), and by the Natural Sciences and EngineeringResearch Council of Canada (to GH). DSL wasthe recipient of an Ontario...
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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt
... deamina-tion of N-methyl-l-alanine with NADP+(0.36 Uặmg)1) was markedly lower than reductive amination of pyruvate with NADPH.Fig. 1. Overexpression and purification of recombinant NMAADH.Protein ... Japan), and New EnglandBiolabs (Beverly, MA, USA). All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture and ... ProteinStructural and Functional Analyses and by Grant-in-Aid from the Ministry of Education, Culture, Sports,Science and Technology, Japan (21st Century COE onKyoto University Alliance for Chemistry).References1...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... Biology(Keio University)’ and a grant from New Energy andIndustrial Technology Development Organization(NEDO) of the Ministry of Economy, Trade andIndustry of Japan (Development of a TechnologicalInfrastructure ... lm PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reactionmixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents ... enzyme consisting of an RNase Hdomain and an acid phosphatase domainNaoto Ohtani1, Natsumi Saito1, Masaru Tomita1, Mitsuhiro Itaya1,2and Aya Itoh11 Institute for Advanced Biosciences,...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... electroblotted and immunodetected by a-SmG-STO serum. (B) RT-PCR analysis of different S. mansoni stages.Reverse-transcribed RNA from miracidia (lane 1), sporocysts (lane 2),cercariae (lane 3) and adult ... S. mansoni. (A) Immunoblottinganalysis of parasite extracts. Each lane contains total protein (50 lg)from cercariae (lane 1), schistosomula (lane 2), sporocysts (lane 3), andadult worms (lane ... of an omega-class glutathioneS-transferasefromSchistosoma mansoni with glutaredoxin-likedehydroascorbate reductase and thiol transferase activitiesJavier Girardini1,*, Alejandro Amirante2,†,...
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Health education in prisons: assessment of an experience with diabetics doc
... importance of water✓ An appropriate hydration with meals✓ Choice beverages in diabetes✓ The importance with physical exercise Other healthier substitutesã Physical activity and its importance ... activities and the development of more ambitious interven-tion programs that improve our patients’ health and quality of life; the creation of an educator network between professional of both ... Rev Esp Sanid Penit 2009; 11: 73-79A Minchón Hernando, JA Domínguez Zamorano, Y Gil Delgado. Health education in prisons: assessment of an experience with diabetics— 78 —low levels of knowledge,...
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