... Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan, ROC2 Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC3 Department of Biochemistry, University of Minnesota College of ... structure-based analysis of staphylococcal nuclease. Proteins: Structure, Functionand Genetics 27, 171–183.15 Flanagan JM, Kataoka M, Fujisawa T & EngelmanDM (1993) Mutations can cause large changes ... reportedthat multiple mutations can cause large changes in the average conformation of denatured proteins. Here weshow that a specific single mutation or removal of a specific fragment can cause large...
... higher value almost 2.5-fold higher than the native cold adapted enzyme (Table 1). The mutantG26 1A/ Y26 9A exhibits an E a almost the same as in the caseofthenativeenzyme(Table1).Thermal inactivation ... gene encoding alkaline phosphatasefrom the Antarctic strain TAB5 [16]. Based on the crystalstructure (at 2.4 A ˚)ofanEscherichia coli alkaline phospha-tase variant with a 28% amino-acid sequence ... from the AntarcticTable 1. Enzymatic and thermodynamic parameters ofthe psychrophilic alkaline phosphatase and mutants. Reported values were determined at10 °C. The kcatvalues were calculated...
... ofthe transistor indicated a spiral model of interac- 50Since 1970, the State has funded both capital investments and the running of the University of Aarhus in the same way as for the other ... regionsmake it impossible to take advantage ofthe full growth potential ofa univer-sity.Another hypothesis is that the size ofthe regional impact ofa university pri-marily reflects the characteristics ... Ålborg and Roskilde, togetherwith the expansion ofthe number of students at the Aarhus School of Busi-ness in the 70s and the 80s, the rate of growth decreased and the number of students was maintained...
... control The way the knowledge transfer programme wasmanaged also differed between plants. The idea of the central programme management was that,at the least, plants should make yearly plans foreach ... (production manager) .Aspiration and strategic ambitionsBecause each plant investigated was run as a profitcentre and was normally measured (by corporateheadquarters) on operating margin and/or ... have. Theroleof motivation isprobably as important in a chain of hotels or super-markets, a software vendor or a consulting com-pany, regardless ofthe nature of knowledge. The method of analysis...
... REPRESENTATIVESJohn B. Bass, Jr., M.D.American Thoracic SocietyUniversity of South AlabamaMobile, ALNancy E. Dunlap, M.D.American College of Chest PhysiciansUniversity of Alabama at BirminghamBirmingham, ... heterogeneity in the efficacy ofthe BCG vaccine reported in the individual studies. Using a model that included the geographic latitude of the study site and the data validity score as covariates, they ... Public Health VeterinariansKeith A. Clark, D.V.M., Ph.D.Texas Department of HealthAustin, TXNational Vaccine ProgramAnthony Robbins, M.D.Office ofthe Assistant Secretary forHealthWashington,...
... usingIMAGEQUANTsoftware, anddepurination was calculated by relating the amounts of the small aniline-fragment and 5.8S rRNA and expressingvalues as a percentage.Reassociation and quantification of ... quantitativeactivity assays of these RTA variants, was not achieved. Toassess whether substitution at Asn78 with Ser changed the catalytic activity of RTA, the N-glycosidase activity of RTAN78S against ... collection andrefinement statistics are given in Table 1.Assay of the N-glycosidase activity of ricin A chainvariants The activity of each ofthe RTA variants was determinedby assessing their ability...
... epitope tag was added t o the end of t he ESSS ORF. The forward primer was: 5Â-ACgaatccGATCTCCGACCCA-3Â; the reverse primer was:5Â-ATgctagcCTCATCTTCTGGTAACTGG-3Â. Small bo ldletters refer to the ... antibodieswere as follows: anti-porin from Calbiochem, anti-HA fromCovance BabCo, anti-mouse and anti-rabbit secondaryantibodies from Bio-Rad Laboratories and AmershamPharmacia Biotech, respectively. Antibodies ... analyses ofthe ESSS cDNA in these mutantsrevealed chain termination mutations. In two of thesemutants the protein i s truncated at the C-terminus of the targeting sequence; the m utants are...
... enterprises,as well as the actual constraints exerted by the structure ofa balance sheet are still limited, a qualitative survey may actually offer a stronger appraisal of their actual behaviors.Section ... determinants of the probability of making profit, but this would be a set of given, ‘frozen’ parameters, rather thanadjustment variables on which the managers’ strategies would actually be able bear. ... would have to take into account the evolution of real wages as well as of wage arrears. However, a remarkable point is that a substantial proportion of firms (31.5% ofthe sub-sample) has increased...
... (Nickel and McLachlan 1994). 38 Function and structural adaptation ofthe condyle Summary ofthe basic anatomical and functional changes in the condylar portion ofthe joint. Increased functional ... subchondral cartilage has not yet been affected and would appear in-tact on a radiograph. 35 Buildup ofthe condylar cartilage Histologically, the secondary cartilage ofthe condyle is made up of ... Neurovascular pain Vascular pain Glandular, ocular, and auricular pain Pulpaf pain Visceral mucosal pain Periodontal pain Connective-tissue pain Ostealgia m6 periosteal pain ...
... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the sameenzyme.To obtain an insight ... regard to theroleof Asp140,whichappearstobecrucialinChiBandinchitinaseA1from Bacillus circulans, whereas it may be mutated toasparagine without loss of activity in other family 18chitinases...
... in the analysis of game-theoretic models (chapter 10) –how one playerresponds to the actions of another on the assumption ofa speci…c form of the rules ofthe game.We use the comparative statics ... equation:q = F(K; L) (“quantity of output = a function of capital and labour”), whichis a convenient way of picking up some ofthe features that are essential toanalysing the behaviour ofthe ... because the method of proofis not particularly illuminating or is rather technical. Throughout each chapter there are footnotes that focus on detailed points of the argument. These take the...
... imbalance, educationalimbalance, and where they are fearful of appearing stupidand fearful of rejection or abandonme nt. As a result, they arehesitant to admit that they do not understand ... literacy amongmedicare enrollees in a managed care organization. JAMA 1999;281:545–51.[10] International Reading Association, Special Interest group on readingand readability. Newark, Delaware, ... novelabout asbestos hazards or an NCI asbestos pamphlet weremailed, with an evaluation questionnaire, to a random sample of 500 members ofa building trades union. There was a 21%response rate...