... Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan, ROC2 Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC3 Department of Biochemistry, University of Minnesota College of ... structure-based analysis of staphylococcal nuclease. Proteins: Structure, Functionand Genetics 27, 171–183.15 Flanagan JM, Kataoka M, Fujisawa T & EngelmanDM (1993) Mutations can cause large changes ... reportedthat multiple mutations can cause large changes in the average conformation of denatured proteins. Here weshow that a specific single mutation or removal of a specific fragment can cause large...
... higher value almost 2.5-fold higher than the native cold adapted enzyme (Table 1). The mutantG26 1A/ Y26 9A exhibits an E a almost the same as in the caseofthenativeenzyme(Table1).Thermal inactivation ... gene encoding alkaline phosphatasefrom the Antarctic strain TAB5 [16]. Based on the crystalstructure (at 2.4 A ˚)ofanEscherichia coli alkaline phospha-tase variant with a 28% amino-acid sequence ... from the AntarcticTable 1. Enzymatic and thermodynamic parameters ofthe psychrophilic alkaline phosphatase and mutants. Reported values were determined at10 °C. The kcatvalues were calculated...
... in the analysis of game-theoretic models (chapter 10) –how one playerresponds to the actions of another on the assumption ofa speci…c form of the rules ofthe game.We use the comparative statics ... equation:q = F(K; L) (“quantity of output = a function of capital and labour”), whichis a convenient way of picking up some ofthe features that are essential toanalysing the behaviour ofthe ... because the method of proofis not particularly illuminating or is rather technical. Throughout each chapter there are footnotes that focus on detailed points of the argument. These take the...
... Ålborg and Roskilde, togetherwith the expansion ofthe number of students at the Aarhus Schoolof Busi-ness in the 70s and the 80s, the rate of growth decreased and the number of students was maintained ... ofthe transistor indicated a spiral model of interac- 50Since 1970, the State has funded both capital investments and the running of the University of Aarhus in the same way as for the other ... Number of students and teaching staff at University of Aarhus1980-2002Source: The University of AarhusIn the same period, there were a number of changes in the admission of stu-dents. Because of...
... transfer and theroleof motivation in the way that we have. Theroleof motivation isprobably as important in a chain of hotels or super-markets, a software vendor or a consulting com-pany, ... nature ofthe programme wasaddressed by many respondents, and it was a par-ticular area where views differed. The programmewas designed as a competition, with of cial resultsand annual award ... programme wasmanaged also differed between plants. The idea of the central programme management was that,at the least, plants should make yearly plans foreach machine, outlining performance targets,...
... Public Health VeterinariansKeith A. Clark, D.V.M., Ph.D.Texas Department of HealthAustin, TXNational Vaccine ProgramAnthony Robbins, M.D.Office oftheAssistant Secretary forHealthWashington, ... REPRESENTATIVESJohn B. Bass, Jr., M.D.American Thoracic SocietyUniversity of South AlabamaMobile, ALNancy E. Dunlap, M.D.American College of Chest PhysiciansUniversity of Alabama at BirminghamBirmingham, ... clinical trials and case-control studies of BCG2 MMWR April 26, 1996 Other BCG preparations are available for treatment of bladder cancer; these prepara-tions are not intended for use as vaccines.Vaccine...
... epitope tag was added t o the end of t he ESSS ORF. The forward primer was: 5Â-ACgaatccGATCTCCGACCCA-3Â; the reverse primer was:5Â-ATgctagcCTCATCTTCTGGTAACTGG-3Â. Small bo ldletters refer to the ... antibodieswere as follows: anti-porin from Calbiochem, anti-HA fromCovance BabCo, anti-mouse and anti-rabbit secondaryantibodies from Bio-Rad Laboratories and AmershamPharmacia Biotech, respectively. Antibodies ... analyses ofthe ESSS cDNA in these mutantsrevealed chain termination mutations. In two of thesemutants the protein i s truncated at the C-terminus of the targeting sequence; the m utants are...
... enterprises,as well as the actual constraints exerted by the structure ofa balance sheet are still limited, a qualitative survey may actually offer a stronger appraisal of their actual behaviors.Section ... would have to take into account the evolution of real wages as well as of wage arrears. However, a remarkable point is that a substantial proportion of firms (31.5% ofthe sub-sample) has increased ... determinants of the probability of making profit, but this would be a set of given, ‘frozen’ parameters, rather thanadjustment variables on which the managers’ strategies would actually be able bear....
... (Nickel and McLachlan 1994). 38 Function and structural adaptation ofthe condyle Summary ofthe basic anatomical and functional changes in the condylar portion ofthe joint. Increased functional ... Position in the Frontal Plane 217 Attaching the Anatomical Transfer Bow 168 Misinterpretation ofthe Disk Position in the Sagittal Plane 220 Mounting the Maxillary Cast using the Anatomical 169 ... subchondral cartilage has not yet been affected and would appear in-tact on a radiograph. 35 Buildup ofthe condylar cartilage Histologically, the secondary cartilage ofthe condyle is made up of...
... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the sameenzyme.To obtain an insight ... regard to theroleof Asp140,whichappearstobecrucialinChiBandinchitinaseA1from Bacillus circulans, whereas it may be mutated toasparagine without loss of activity in other family 18chitinases...
... imbalance, educationalimbalance, and where they are fearful of appearing stupidand fearful of rejection or abandonme nt. As a result, they arehesitant to admit that they do not understand ... literacy amongmedicare enrollees in a managed care organization. JAMA 1999;281:545–51.[10] International Reading Association, Special Interest group on readingand readability. Newark, Delaware, ... novelabout asbestos hazards or an NCI asbestos pamphlet weremailed, with an evaluation questionnaire, to a random sample of 500 members ofa building trades union. There was a 21%response rate...