... conformation due to the loss of astabilizing constraint onthe ground state ofthe receptor.Therefore, the agonist had a lower affinity than the wildtypeV1aR but once the agonist bound, the receptor ... 270) Ó FEBS 2003Results The effect of systematic substitution of Arg46 on agonist binding The structural requirements ofthe residue at position 46 in the N-terminus ofthe V1aR (Fig. 1) were ... protein. In contrast to the three classes of antagonist, the binding of AVP was dramatically affectedby the substitution of Arg46, with the affinity of AVP for all the 19 mutant receptor constructs...
... implemented in the baseline. The amounts ofthe payments are considered a simulation outcome, because they depend on production and they are thus presented in the section of scenario results. The core ... assessment of possible implications ofthe dairy policy reform, with an explicit focus on regional effects in the EU-27 of a milk quota abolition in the year 2015. Specification ofthe Model The ... others are open to all countries under the Most-Favoured Nations (MFN) treatment of the WTO. There are some regional exceptions to the operation ofthe MFN tariffs, such as for example the Everything...
... phosphorylation.Effect of RS motif phosphorylation on oligomerization ofthe N proteinTo better understand the effect of RS motif phosphor-ylation onthe biological function ofthe N protein, ... together,these data suggest that the RS-rich region ofthe SARS-CoV N protein is possibly phosphorylated by SRPK1.Phosphorylation of multiple serines within the RS motif The RS motif ofthe ... cellular functions.Multimerization ofthe N protein is necessary fornucleocapsid formation and assembly ofthe viralparticles [42]. Thus, whether phosphorylation of the RS motif in virions could...
... is the angle between the projection ofthe tilt ofthe z axis onthe x¢,y¢ plane (defined direction of tilt of z and the x¢ axis), and j % a + c defines the location ofthe rhombic (x, y) axes. The ... cyanide. The observation of a significant iso-tope effect onthe heme methyl contact shifts confirms astrong contact between the Tyr32(B10) OH and the ligatedcyanide. The quantitative determination of ... relaxationproperties ofthe CeHs signal (see above). The hydroxylproton position is not directly determined in the crystalstructure, but can be inferred by the position of other H-bond acceptor/donors in the...
... position 2. The substitution positions of all the other monosaccharideswere confirmed by GC-MS data ofthe methylated productsto be as presented in Scheme 1.Deamination ofthe products of complete ... have the D-configuration, and Fuc had the L-configuration. 3-O-Methyl-6-deoxytalose had a positiveoptical rotation, which confirms itsD-configuration.However, the value ofthe optical rotation ... acetylated. The second procedure led to the reduction ofthe GlcA residuewith the introduction of two deuterium labels at C6.Comparison ofthe two chromatograms allowed unambi-gous confirmation that...
... recorded during addition ofthe first electron(ox/sq transition), whereas dotted lines shows spectra recorded during addition ofthe second electron (sq/hq transition). The tendency ofthe proteinto ... concentration ofthe FMN domain is increased indicatesthat the reaction rate is controlled by some process otherthan collision ofthe two flavin-binding domains.Discussion The ability ... addition ofthe firstelectron (oxidized-to-semiquinone transition) are indicated by thin, solid black lines. Spectra collected during addition ofthe second electron to the flavin (semiquinone-to-hydroquinone...
... the Dconfiguration (the b effect on C3 of L-GlcAwouldbeclosetozero[27]). On the basis ofthe data obtained, it was concluded that the repeating unit ofthe O-polysaccharide of P. myxofac-iens has the following ... 61.9, and 62.1,respectively [26], demonstrated the modes of glycosylation of the monosaccharides. The linkage positions were confirmed and the sequence of the monosaccharides was determined using ... The Dconfiguration of GlcN andGalN was established by GLC ofthe acetylated (S)-2-butylglycosides. The Dconfiguration of GlcA was determined byanalysis of the 13C NMR chemical-shift data of the polysaccharide...
... amounts ofthe ECDs ofthe non-a sub-units of human muscle AChR for use as starting material for the determin-ation ofthe 3D structure ofthe receptor ECDs and for the characterization of the ... expression of c1–219. The presence of the HIS tag alone reduced the expression of both con-structs by 30–40% (Fig. 1B, bars 2 and 6), and furtheraddition of FLAG to c1–218HIS gave 100% expression(construct ... Purification and deglycosylation ofthe AChR ECDs. (A) Schematic representation ofthe constructs used for expression of the b1–221, c1–218 and e1–219 ECDs ofthe human AChR in yeast P. pastoris. The...
... the second residue of the primary sequence; the second series consists of a progressivetruncation ofthe C-terminal domain of PufX (Fig. 1)obtained by the introduction of stop codons in the genesequence ... prior to the onset ofthe reaction at its maximal rate. In wild-type ICM, the initial rate of this reaction, as well as the lag phase,depends onthe redox state ofthe ubiquinone pool and on the ... (QB). The full reduction of the quinone m olecule at QBto quinol requires a secondphotoexcitation ofthe RC and is coupled to the uptake of two protons from the cytoplasmic space. The formedubiquinol...
... in the U.S. devoted to education, but there is no substantiation of this figure, and most ofthe blogs discuss are professional or academic blogs. There is an extended discussion of nonprofit ... account ofthe increasing failure ofthe news ecosystem to adequately report on education nationally and locally, with some suggestions for the formation of a non-profit reporting sector. The third, ... secondary analysis. Although they are a strong overview ofthe state of education journalism, the studies are only a beginning. The fact that they stand out as they do reflects not only their...
... at the highest lipidconcentrations, while the monotonic increase and lowoverall enhancement of LLP-2 and LLP-3 indicates thatthey are only partially bound at these lipid concentra-tions. The ... insertion andinteractions at the membrane-water interface in which the peptides lie onthe cell surface to cause a generalized dis-ruption ofthe membrane. However, our observation of nearly ... is the con-centration of water (55.3 M). The fraction of peptidebound in any experiment can be calculated using the equationFraction bound = (Kx × [L])/([W] + (Kx × [L]))In the case of...
... complete partition-ing determined by fitting the equation to the experimentaldata, [L] is the concentration of lipid and [W] is the con-centration of water (55.3 M). The fraction of peptidebound ... at the highest lipidconcentrations, while the monotonic increase and lowoverall enhancement of LLP-2 and LLP-3 indicates thatthey are only partially bound at these lipid concentra-tions. The ... inosmolysis. The ability of UV-inactivated HIV to cause sin-gle cell balloon degeneration in the absence of replicationargues for the involvement of a virion component, possi-bly the LLP domains...
... are one ofthe most common types of bacteriophages with 26–29 defined or tentative speciesaccording to the VIII report ofthe International Commit-tee onthe Taxonomy of Viruses [3,4]. Most of ... A). The masses ofthe proteins are indicated in the adjacent lanes. The tentative identification based on in silico analysis ofthe properties ofthe gene products (Gp) are indi-cated onthe ... above that ofthe major capsid protein(Gp10) which was not immediately linked, onthe basis of its mass, to a product of one ofthe morphogenesis genes.One ofthe characteristics of some T7-like...
... and these appear to result fromreduction/expansion of the thymidine-rich region at the 5’ end. Excluding the variable sites associated with these thymidine regions, the ... misincorporation. The occurrence of this minimal intra-individual variation and the identification of the same restriction patterns afterenzyme digestion in the individuals ... pseudogenes among the repeat units analysedhere can be considered improbable.In the spacer region of 5S rDNA, three of the clones analysed are almostidentical as only two...