the polymerase chain reaction
A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
Ngày tải lên :
06/03/2014, 04:20
... hospital patients having a disease other than tuberculosis The latter samples were controls for all the investigations carried out on the test samples Of the 123 samples, 97 samples were taken ... diagnosed as not having tuberculosis, the PCR profile of the individual had changed markedly, suggesting an increase in the bacterial load in the sputum Only seven of the 17 cases i.e those who took ... numbers need to be taken up in order to validate these results In this context, a recent study examined the cost-effectiveness of polymerase chain reaction versus Ziehl-Neelsen smear microscopy...
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Báo cáo nghiên cứu khoa học: " ỨNG DỤNG KỸ THUẬT PCR (POLYMERASE CHAIN REACTION) ĐỂ PHÁT HIỆN NHIỄM SẮC THỂ PHILADELPHIA TRÊN BỆNH NHÂN UNG THƯ BẠCH CẦU MÃN TÍNH DÒNG HẠT (CHRONIC MYELOID LEUKEMIA )" pptx
Ngày tải lên :
23/07/2014, 00:21
... blood samples In these 59 positive cases, the b3a2 type has the highest rate of 44,07%, followed by the b2a2 type with 32,2%, and the b2a2 + b3a2 combinative type with 23,73% These results indicate ... Trancription -Polymerase Chain Reaction) and Real-Time PCR to detect Philadelphia chromosome containing BCR-ABL fusion gene Results show that the BCR-ABL fusion gene was found in 59 (98,33%) of these ... [2] Khuếch đại týp gene tổ hợp BCR-ABL b2a2 b3a2 kỹ thuật RT-PCR (Reverse Transcription -Polymerase Chain Reaction) sau tiến hành xét nghiệm định lượng Real-Time PCR nhờ 03 nồng độ chuẩn gen β-glucuronidase...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
Ngày tải lên :
07/08/2014, 18:20
... RD, Watson R, Gelfand DH Detection of specific polymerase chain reaction product by utilizing the 5-3 exonuclease activity of Thermus aquaticus DNA polymerase Proc Natl Acad Sci USA 1991, 88, 72767280 ... results but the primer sets of prM and 5' NTR didn’t Therefore, primers and probe of 3' NTR for the diagnostic purpose of JEV were selected The concentration of primers and probe giving the highest ... more sensitive than RT-PCR assay When the same person performed the real-time RT-PCR three times at the different day, the same results were obtained from all the three trials (Fig 3B) 348 Dong...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
Ngày tải lên :
09/08/2014, 01:24
... transcriptase polymerase chain reaction The expression levels of the messages in OA and RA are expressed by longitudinal bars relative to those of the control value expressed as 1.0 The lines outside the ... intensity The CT value of the βactin message was used as an internal standard When the target messages were detected in both the control samples and the OA or RA samples by RT-PCR, the ratio for the ... time when the annealed probes were broken by DNA polymerases during the polymerization period The threshold cycle numbers (CT), from which the logarithmic amplification phase of the PCR reaction...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
Ngày tải lên :
12/08/2014, 02:20
... probably due to the reactivation of the latent virus present in them or other invasive procedures that lead to transmission of HCMV In the sub-group III of Group A since the donor’s and the recipient’s ... from the study The mean age of the recipients was 48.5 year and the female to male ratio was 2:3 The age range among recipient males was 23 to 56 and female was 23 to 59 The mean age of the donors ... was copies/ml The highest positive pp65 cell count recorded among them was 78 cells and the lowest was cells In eleven others, the blood sample was positive only by real - time PCR The highest...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
Ngày tải lên :
12/08/2014, 04:20
... primer/probe A reaction system B: amplification plot of the HCV sample in the singleplex primer/probe B reaction system C: amplification plot of the HCV sample in the duplex primer/probe A and B reaction ... detect these samples in theory, regardless of the nucleotide polymorphisms Second, the duplex primer/probe assay can estimate the virus levels accurately There are many reports about the underestimation ... detected the HCV 5' UTR sequence Further, both the detection probes were labelled with the same fluorophore, i.e FAM, at the 5' end and with the same quencher dye, i.e Black Hole Quencher (BHQ), at the...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
Ngày tải lên :
12/08/2014, 04:20
... the scope of other diagnostic methods The real-time PCR assay permits the simultaneous detection and quantification of DNA It is useful for understanding the pathogenesis of the disease and the ... differentiate the GPV vaccine strain from the high-virulence strain; nonetheless, we could perform the study on the dynamic distribution of GPV in vivo using these methods, because the animals ... template The optimized annealing temperature was 52°C Recently, the highly conserved VP3 region of the GPV gene was cloned and sequenced and analyzed by qualitative polymerase chain reaction...
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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
Ngày tải lên :
12/08/2014, 04:21
... to determine the subtypes of dengue virus circulating in the island over the last five years by molecular techniques and so assess the efficacy of this method in the adaptation of the current ... single serum sample does not provide information on the serotype of the virus However, single-step reverse transcriptase polymerase chain reaction (RT-PCR) detection and typing of dengue virus ... serum sample [7,8] The diagnosis of dengue fever by this one-step RT-PCR procedure has reduced the amount of time in getting the results to the patient In comparison with the IgM ELISA or IgG...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
Ngày tải lên :
12/08/2014, 18:22
... marker between leukocytes and the others and D marker between fibroblasts and the others The summary of the individual variations mentioned above is shown in Fig Discussion The AMP PCR is a PCR based ... 4°C Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) The PCR reaction consisted of DNA sample (genomic or digested template), Taq polymerase enzyme (AmpliTaq® Stoffel fragment, ... of the applied enzyme that allows assessing of DNA methylation status of the particular locations throughout the genome The alterations of PCR products in the digested DNA template provide the...
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Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection" pptx
Ngày tải lên :
12/08/2014, 23:23
... considered the gold standard for the detection of respiratory viruses, but its limitation is that it can only detect a small number Furthermore, the yield of viral culture depends on the quality ... sepsis at the time of admission The study described here was conducted as part of normal patient care; therefore, according to the Medical Ethical Research Council of our institution, there was ... and data analysis, and revised the report NJ participated in the design of the study, directed the acquisition and interpretation of clinical data, and revised the report AvL played a substantial...
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Báo cáo y học: "Bench-to-bedside review: The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection" pptx
Ngày tải lên :
13/08/2014, 16:20
... in the application of polymerase chain reaction to the diagnosis and management of sepsis The application of PCR techniques to detect and identify pathogens has the potential to revolutionise the ... time-critical diagnostics, are being investigated Polymerase chain reaction approaches to the diagnosis of pathogenaemia Molecular methods based on polymerase chain reaction (PCR) technology have been developed ... determined by the design of oligonucleotide primers, short pieces of synthesised DNA that bind to either end of the sequence and form the starting point for DNA replication by DNA polymerase For...
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Phát hiện loài Fusarium spp. Gây bệnh thối xương rồng (Cactaceae) bằng phương pháp Polymerase Chain Reaction
Ngày tải lên :
17/11/2012, 09:45
... DANH SÁCH CÁC CHỮ VIẾT TẮT PCR :Polymerase chain reaction DNA :Deoxyrybonucleotide acid TE :Tris EDTA TAE :Tris Acetate EDTA dNTP :Deoxynucleotide triphosphate Taq :Thermus aquaticus UV :Ulta violet ... tính giả Enzyme DNA polymerase dùng phản ứng PCR phải enzyme chịu nhiệt cao: Taq polymerase đƣợc tách chiết từ vi khuẩn Thermus aquaticus (vi khuẩn suối nƣớc nóng) Taq polymerase cao thấy tƣợng ... PHÁT HIỆN LOÀI NẤM Fusarium spp GÂY BỆNH THỐI XƢƠNG RỒNG (Cactaceae) BẰNG KỸ THUẬT PCR (Polymerase Chain Reaction) Giáo viên hƣớng dẫn KS DƢƠNG THÀNH LAM TS LÊ ĐÌNH DÔN Sinh viên thực PHẠM THỊ...
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ỨNG DỤNG PHƯƠNG PHÁP PCR (POLYMERASE CHAIN REACTION) VÀ PHƯƠNG PHÁP NUÔI CẤY ĐỂ KHẢO SÁT SỰ NHIỄM VI SINH VẬT GÂY BỆNH TRONG THỰC PHẨM ĐƯỜNG PHỐ
Ngày tải lên :
16/03/2013, 09:26
... PCR (Polymerase Chain Reaction) Phương pháp PCR phương pháp in vitro để tổng hợp DNA dựa khn trình tự đích DNA ban đầu, khuếch đại, nhân số lượng khn thành hàng triệu nhờ hoạt động enzyme DNA polymerase ... thuật PCR (Polymerase Chain Reaction) PCR phản ứng tổng hợp dây chuyền dùng để khuyếch đại trình tự DNA đích dựa bắt cặp đặc hiệu mồi với DNA mạch khn hoạt tính kéo dài cuả enzyme polymerase ... phố ngày quan tâm [43] Chính lí trên, chúng tơi chọn đề tài “Ứng dụng phương pháp PCR (Polymerase Chain Reaction) phương pháp ni cấy để khảo sát nhiễm vi sinh vật gây bệnh thực phẩm đường phố”...
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Salmonella trong sản phẩm thuỷ sản - Phương pháp định tính bằng kỹ thuật Polymerase Chain Reaction
Ngày tải lên :
20/03/2013, 08:34
... có trình tự bổ sung để tạo nên mạch kép 3.6 ADN polymerase enzym tổng hợp nên mạch ADN từ mạch khuôn Phương pháp sử dụng Taq ADN polymerase ADN polymerase hoạt động nhiệt độ cao 3.7 Mồi trình ... AND đích qua chu kỳ gồm bước: biến tính ADN, bắt cặp bổ sung với mồi tổng hợp mạch nhờ enzym ADN polymerase 3.3 ADN đích (target ADN) Trong kỹ thuật PCR, ADN đích hiểu đoạn ADN đặc trưng cho đối ... cao 3.7 Mồi trình tự ADN hay ARN ngắn bắt cặp với mạch khuôn ADN có mang đầu 3' - OH tự giúp ADN polymerase bắt đầu tổng hợp mạch Nguyên tắc Phương pháp định tính dựa vào việc xác định đoạn ADN...
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KỸ THUẬT PCR (POLYMERASE CHAIN REACTION)
Ngày tải lên :
24/04/2013, 15:41
... ADN polymerase Là enzym xúc tác cho trình lắp ráp nucleotit A, T, G, C vào mạch ADN tổng hợp Ngày người ta dùng nhiều loại ADN polymerase: Taq ADN polymerase, Pfu ADN polymerase, T4 ADN polymerase, ... ADN Từ đó, không cần phải thêm ADN -polymerase vào chu kỳ, trình chép ADN đơn giản tự động Một ADN -polymerase chịu nhiệt phân lập từ Thermus aquaticus gọi Taq Taq polymerase dùng rộng rãi thực nghiệm ... quy trình gốc phát triển cách dùng ADNpolymerase lấy từ vi khuẩn Thermus aquaticus (ưa nhiệt) sống mạch nước phun nhiệt độ 110°C ADN polymerase từ sinh vật thermostable (ổn định nhiệt độ cao) dùng...
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Luận văn xây dựng quy trình xác định vi khuẩn verotoxigenic escherichia coli trong thịt bằng kỹ thuật PCR (polymerase chain reaction
Ngày tải lên :
27/11/2013, 22:18
... cho nghiên c u ti p theo Vì v y, ñã ti n hành nghiên c u ñ tài: “Xây d ng quy trình xác ñ nh vi khu n Verotoxigenic Escherichia coli th t b ng k thu t PCR (Polymerase chain reaction) ” Nghiên c ... LB Luria Bertani MR Methyl Red m-TSB modified Trypticase Soy Broth NB Nutrient Broth PCR Polymerase chain reaction (Ph n ng chu i trùng h p) TTP Thrombotic thrombocytopenic purpure (Ban xu t huy ... pháp ñã ñư c nghiên c u thí nghi m thành công, ph m vi báo cáo này, ch ñ c p t i k thu t Polymerase chain reaction (PCR) S ñ i c a k thu t PCR ñã ñưa l i m t cu c cách m ng vi c nghiên c u, phân...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
Ngày tải lên :
10/02/2014, 20:39
... Viruses in the Upper Respiratory Tracts of Mice, PLoS Pathog 30 Hoa, L.T., D.D Khang, P.V Chi, N.V Hai, T.N Hai, N.T.B Nga, and L.T Binh (2006), “Molecular characterization of the H5 gene for the highly ... “Orthomyxoviridae: The Viruses and Their Replication”, Fields Virology, 4th Edition, (D.M Knipe and P.M Howley, eds), pp 1487-1531 37 Lamb RA (1989), Genes and proteins of the influenza viruses ... (2008), The development and genetic diversity of H5N1 influenza virus in China, 1996-2006”, Virology, 380, pp 243-54 23 Gambaryan A, Tuzikov A, Pazynina G, et al (2006), “Evolution of the receptor...
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pcr (polymerase chain reaction)
Ngày tải lên :
19/02/2014, 10:05
... PCR (Polymerase chain reaction) I Những kiến thức PCR PCR từ viết tắt cụm từ Polymerase Chain Reaction tiếng Anh Có nhiều cách dịch cho cụm từ Có sách dịch phản ứng tổng hợp dây chuyền nhờ Polymerase, ... vy Chớnh õy m mt loi sinh vt, Thermus aquatics v ADN polymerase liờn kt ca nú tr thnh quan trng Loi ADN polymerase ny n nh vi nhit (heat-stable) v c gi l Taq ADN polymerase Vi khun a nhit tỡm ... nớc nóng có ADN polymerase làm việc tốt đợc nhiệt độ cao Vi khuẩn Thermus aquaticus sống nớc có nhiệt độ 75oC (sinh sng sui nc núng cụng viờn quc gia ỏ vng) ADN polymerase (Taq polymerase) có...
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