... CCAAT box at 324 bp upstream of the major transcription start site. The presence of a CCAATbox and the absence of a TATA box seem to be one of the characteristics ofthe mouse integrin a3 gene.We ... Wepreviously reported the structures ofthe mouse a3integrinsubunit gene including the exon/intron organization and the alternative exon usage for the generation of variants of the a3 subunits (a3A ... upstream ofthe SacI) sites (Fig. 1).Sequence analysis ofthe 5¢-flanking region anddetermination of transcription start sites of mouseintegrin a3 subunit gene The nucleotide sequence ofthe 0.5...
... the beginning ofthe twentieth century there was but little night to speak of, save in the sense of a division of time. The date ofthe paper which con-tained the account ofthe tragedy on the Russian ... ran across the end at which the arm-racks were, and onthis Arnold laid the case containing the model, he standing on one side of the table, and the members ofthe Circle on the other, watching ... the two new strangely met and strangely assorted friendswalked away together in the direction ofthe Savoy. The suite of rooms occupied by Arnold's new acquaintance was the beau ideal of...
... imagine the old knight, onlyhalf aware ofthe sunshine ofthe evening, the noise ofthe streets, the looks ofthe crowd, the great minsterrising half-finished in the midst ofthe town by the Rhine, ... Boiardo, ofthe Ferrarese court to which he belonged, and ofthe school of poetry (including the more notable but less original work of Ariosto) which the genius ofthe man and the character ofthe ... this: on the extreme pinnacle, high on the roof, they might indeed place against the russet brick or the blue sky, amid the hum of life and the movement ofthe air, the living man, like the Scaligers,...
... mutation of Arg46 had the dualeffect of (a) decreasing agonist affinity and (b) promoting the agonist-induced active conformation due to the loss of astabilizing constraint on the ground state ofthe ... with the corresponding sequence of the V1aR [10]. The role ofthe N-terminus has beenaddressed recently by alanine-scanning mutagenesis of the N-terminus ofthe V1aR; this revealed that a ... transmembrane domains traversing the lipid bilayer. The enlarged circle shows the amino acid sequence ofthe distal N-terminus of the V1aR and indicates the position of Arg46 that was subjected...
... Impact ofthe Abolition ofthe Milk Quota Regime – Regional Analysis ofthe Milk Production in the EU" (AGRI-2007-0444). The project aimed at a thorough policy impact analysis ofthe EU ... through either the transfer of an entire farm, the leasing or purchase of quota, or the allocation of quota from a national reserve. The transfer of milk quota may take place via a variety of administrative ... average, with +35.5% in the EU-10 and +13.8 in the EU-15. This excess supply makes the net trade position ofthe EU-27 stronger towards the Rest ofthe World. Within the EU there is a quite heterogeneous...
... copies ofthe N protein, respectively. (C) The RNA binding efficiency of N protein is represented as a percentage of bound RNA (i.e. the percentage of bound RNA = 100%)percentage of free probe). The ... phosphorylation.Effect of RS motif phosphorylation onoligomerization ofthe N proteinTo better understand the effect of RS motif phosphor-ylation on the biological function ofthe N protein, weFig. 3. The translation ... that of the full-length N protein (data not shown). Taken together,these data suggest that the RS-rich region ofthe SARS-CoV N protein is possibly phosphorylated by SRPK1.Phosphorylation of...
... the projection ofthe tilt ofthe z axis on the x¢,y¢ plane (defined direction of tilt of z and the x¢ axis), and j % a + c defines the location ofthe rhombic (x, y) axes. The orientation ofthe ... at position E7 as the source ofthe H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... ligand.Comparison ofthe strengths of dipolar interactions insolution with the alternate crystal structures of methemo-globin establish that the solution structure of wild-type Hbmore closely resembles the...
... and showed, in particular, the configuration of ManNAc to beD.From the combined the data on the structures of the isolated oligosaccharides, the overall structure of the P. frisingensis LPS carbohydrate ... with 7a (Table 1) because ofthe b-effect ofthe acetylation. Acetylation of 7b was confirmed byESI MS data, which gave the expected mass of 3221.4 Da. The spectra of oligosaccharide 6 were ... 3-O-methyl-6-deoxytalose, as setting the L-isomer instead of the D-isomer resulted in the disappearance ofthe contactbetween protons U6 and P4.To determine the absolute configurations ofthe mono-saccharides,...
... FAD/NADPH domain(% 16-fold) (29). However, the domain mixture still recon-stituted only % 2% ofthe activity ofthe full length CPR. InthecaseofNR1,thekcatfor the full length enzyme is low(1.3 s)1) ... enzymes, the potentials for the FAD/NADPHdomains are rather more negative than those for the FMNdomain, reflecting the direction of electron transfer fromNADPH through FAD, then FMN and on to the ... BM3 [28]. Aggregation ofthe NR1FAD/NADPH domain was much less extensive in the absence of cofactor reduction.Stopped-flow kinetic studiesReduction ofthe FAD/NADPH domain of NR1 wasinvestigated...
... LPS)[5,6]. The serological specificity ofthe bacteria is definedby the structure ofthe O-specific polysaccharide chain(O-antigen) ofthe lipopolysaccharide. On the basis of the O-antigens, the strains ... the Dconfiguration (the b effect on C3 of L-GlcAwouldbeclosetozero[27]).On the basis ofthe data obtained, it was concluded that the repeating unit ofthe O-polysaccharide of P. myxofac-iens has the following ... 2.01–2.10.These data together suggest that the tetrasacchariderepeating unit ofthe polysaccharide consists of two residues of GlcNAc and one residue each of GalNAc, GlcA andAlaLys. The 1Hand13C...
... amounts ofthe ECDs ofthe non-a sub-units of human muscle AChR for use as starting material for the determin-ation ofthe 3D structure ofthe receptor ECDs and for the characterization of the ... datafrom electron images ofthe AChR found in largeamounts in the electric organ ofthe marine ray,Torpedo californica [3]. The acquisition ofthe crystallo-graphic structure ofthe mollusc acetylcholine-bindingprotein ... domain [3]. The N-terminal ECD of each ofthe two a subunits con-tains the major part ofthe binding site for the cho-linergic ligands. The two sites are nonequivalent, onebeing formed at the interface...
... demonstrated the role of PufX in the orientation ofthe RC inside the LH1Fig. 4. Isolation ofthe PMCs on a sucrose gradient. The final detergentextracts from the ICM were loaded on the top of a 10–40% ... PufXD2)7neither affects the photo-synthetic capability ofthe bacteria nor the efficiency of exchange ofthe ubiq uinol molecules between th e RC and the bc1, a s judged from the reduction kinetics of ... residues ofthe LH1 apolypeptide in the organization ofthe LH1–RC complex[12]. In the present work, we have investigated the possible involvement ofthe N-terminus and of the C-terminus of PufX...
... power, in the beauties of holiness; from the womb ofthe morning thou hast the dew of thyyouth. There were many evident proofs ofthe truth of this since the beginning ofthe plantation ofthe gospelinto ... First, The uncertainty ofthe subject. Second, The ambiguity of one phrase.1. The uncertainty ofthe subject, or person of whom the prophet speaks here: whether of Christ, by way of prophecy, or of ... and feet, Europe, and the toes the Isles ofthe Sea the British Isleswith the rest. And the form ofthe great earth-filling figure is that of Jesus of Nazareth, the Man of Jehovah'sright...