the overall cell reaction is h2 1 2 o2 æ h2o and equations 1 83 are applicable s
Báo cáo sinh học: "The ‘stem cell’ concept: is it holding us back" pptx
Ngày tải lên :
06/08/2014, 19:21
... properties that are universally discussed are ‘potency’ and ‘self-renewal’ Stem cells, it is said, display traits of potency and self-renewal that set them apart from other cells Understanding the ... stem cell- driven tissue maintenance in the face of stochastic stem cell behaviors is clearly implemented in at least some tissues The jury is still out on whether all self-maintaining tissues ... about seven cell cycles Traditionally, the all-asymmetric-division model is seen as the simpler of the two, and it is certainly the more popular in textbooks The suggested mechanism is that stem cells...
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Báo cáo khoa hoc:" The CTGF -945GC polymorphism is not associated with plasma CTGF and does not predict nephropathy or outcome in type 1 diabetes" pptx
Ngày tải lên :
11/08/2014, 07:21
... (22 ) 384 (10 0) Yes ( 31) (46) (23 ) 13 (10 0) No 89 (22 ) (14 ) 41 (10 0) 11 1 (29 ) 18 1 (48) 86 (23 ) 21 (38) 25 (45) (16 ) 55 (10 0) 10 5 (29 ) 17 6 (48) 83 (23 ) (0) (10 0) (0) (10 0) 12 6 (30) 20 0 (48) 92 (22 ) ... 25 (22 ) 11 5 (10 0) No Mortality Cardiovascular (CV) death 17 0 ( 51) 77 (23 ) 46 (47) 23 (23 ) 98 (10 0) 97 (28 ) 17 4 (50) 79 (22 ) 350 (10 0) Yes 14 (40) 15 (43) (17 ) 35 (10 0) 11 2 (29 ) 18 6 (48) 86 (22 ) ... genotype and allele frequencies for the CTGF promoter polymorphism at -945 Genotype frequencies (%) Total P-value 12 6 (30 .1) 25 2 (29 .0) 0.4 81 22 0 (49 .1) 2 01 (48.0) 4 21 (48.6) 10 2 (22 .8) 92 ( 21 . 9) 19 4...
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Báo cáo y học: "Human embryonal epithelial cells of the developing small intestinal crypts can express the Hodgkin-cell associated antigen Ki-1 (CD30) in spontaneous abortions during the first trimester of gestation" ppsx
Ngày tải lên :
13/08/2014, 22:22
... testis and mediastinum [22 ] CD30 expression has also been reported in other nonlymphoid tissues and cells such as soft tissue tumours [23 ], decidual cells [24 ,25 ], lipoblasts [26 ], myoepithelial ... spontaneous abortions during the first trimester of gestation Clin Exp Obstet Gynecol 20 01, 28 :22 5 -22 8 Sohail D, Simpson RH: Ber -H2 staining of lipoblasts Histopathology 19 91, 18 :19 1 Mechtesheimer ... Modelling 20 05, 2 :1 gut, kidney, erythropoietic tissue, muscle, bone and connective tissue) from fetuses of 11 and 12 weeks gestational age, with negative results This is the first demonstration...
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Báo cáo khoa học: The equinatoxin N-terminus is transferred across planar lipid membranes and helps to stabilize the transmembrane pore pot
Ngày tải lên :
23/03/2014, 10:20
... modified ver+ sions of S1 C (S1 C-MTSEA , S1 C-MTSET+, S1 C– MTSES , in which S1 C was chemically modified with MTSEA+, MTSET+ and MTSES–, respectively), and His6–EqtII were able to form pores in PLMs at final ... most of the histidines are protonated and thus the N-terminus of His6–EqtII carries a large excess of positive charge Consistently, the rates of closure and opening of the pore, as well as the ... environment [24 ] The question remains of how the region that comprises residues 1 10 is organized in the final pore, and what the nature of the contacts is between the monomers, which should stabilize the...
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Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
Ngày tải lên :
23/03/2014, 20:22
... glutathione-Sepharose as described previously [30] Bacterial two-hybrid system Bacterial strain BTH1 01 [F–, cya-99, araD139, gal15, galK16, rpsL1 (Strlr), hsdR2, mcrA1, mcrB1] and plasmids pKT25kan and pUT18Camp ... proteolysed at Lys 615 -Ala 616 and Arg 620 -Ala6 21 by 14 8 S. -i Yamada et al (Eur J Biochem 27 0) trypsin [20 ,28 ], the border between the middle and C-terminal domains in the present study was set to Lys 618 -Leu 619 ... proteins, such as MyoD and E 12 [43] The findings in the present study on the client binding are consistent with these reports Human GRP94 and mouse HSP90 were identified as tumor-specific antigens expressed...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Ngày tải lên :
19/02/2014, 05:20
... cells Cells were treated for 24 h with each of two siRNAs against HO -2 (siHO -2 and HO-2siRNA1), scrambled siHO -2 (scramble) or siGAPDH Other methods are the same as in Fig Expression levels of ... scrambled HO -2 siRNA did not affect the expression of HO -1 and HO -2 mRNAs and proteins in HeLa and HepG2 cells (Fig 5) Thus, the induction of HO -1 is due to the selective repression of HO -2 expression ... for 12 h after the transfection with siHO1, siHO2, or siGAPDH, then treated with lm hemin for 12 h, and harvested for heme measurement SiRNA and expression plasmids of HO -1 and HO -2 A specific siRNA...
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Báo cáo y học: " PDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein is essential for the interleukin 2 independent growth induction of a T-cell line" ppsx
Ngày tải lên :
13/08/2014, 09:21
... not for citation purposes) Retrovirology 20 05, 2: 46 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Sugamura K, Hinuma Y: Human retroviruses:HTLV-I and HTLV-II In The Retroviridae Volume ... tumor suppressor [33-36] These results present an attractive hypothesis that PBM is a factor responsible for the distinct pathogenic activities of HTLV -1 and HTLV2 Since HTLV -1 is a T -cell- tropic ... HTLV -1 and HTLV -2 encode functionally and structurally similar proteins, Tax1 and Tax2, respectively [7 ,11 , 12 ], and they are candidate factors responsible for distinct pathogenic activities of the...
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Báo cáo y học: "PARP-1 inhibitors: are they the long-sought genetically specific drugs for BRCA1/2-associated breast cancers"
Ngày tải lên :
31/10/2012, 16:57
... repairs single strand breaks (SSBs) in DNA These SSBs result in formation of double strand breaks (DSBs) when they meet replication forks (B) In the presence of wild type BRCA1 and BRCA2, DSBs can ... BRCA1-associated tumorigenesis: what have we learned from knockout mice? Trends Genet 20 01; 17 : S1 8 -22 Deng CX, and Brodie SG Roles of BRCA1 and its interacting proteins Bioessays 20 00; 22 : 728 -37 ... Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, USA Conflicts of interest The authors have declared that no conflict of interest exists References 10 11 12 ...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
Ngày tải lên :
14/02/2014, 19:20
... (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS C Forzani et al 10 11 12 13 14 15 16 17 18 CS, Hirt H et al (20 04) OXI1 kinase is necessary for oxidative burst-mediated signalling ... These results suggest FEBS Journal 27 8 (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 11 29 PTI1-4, a common target of OXI1 and MAPKs A C Forzani et al HIS-OXI > HIS: A OXI ... Mol Cell 15 , 14 1 15 2 FEBS Journal 27 8 (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 11 35 PTI1-4, a common target of OXI1 and MAPKs C Forzani et al 34 Takahashi F, Yoshida...
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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx
Ngày tải lên :
16/02/2014, 15:20
... knockouts The inserts show the cadmium and zinc energy shifts between samples FEBS Journal 27 7 (2 010 ) 25 31 25 42 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 25 35 C elegans metallothioneins discriminate ... but are not copy-edited or typeset Technical support issues arising from supporting information (other than missing files) should be addressed to the authors FEBS Journal 27 7 (2 010 ) 25 31 25 42 ª 2 010 ... homeostasis [15 ], and response to oxidative stress [16 ] There is growing evidence that the existence of multiple MT isoforms is associated with functional differentiation, for example in snails [17 ],...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
Ngày tải lên :
21/02/2014, 01:21
... analysis, subcloning, DNA digestions, and lambda phage and plasmid DNA isolations [23 ] Chromosomal DNA was isolated as previously described [24 ] Sequence analysis was performed on both strands of ... tubingensis [15 ], A aculeatus [ 12 ], Bacillus circulans (Acc No P48843), Yersinia pestis [14 ], Bacillus subtilis (Acc No O07 013 ), Clostridium acetobutylicum [13 ], and Pseudomonas fluorescens [11 ] (Fig ... Microbiol 27 , 13 1 14 2 42 Bos, C.J., Debets, A.J.M., Swart, K., Huybers, A., Kobus, G & Slakhorst, S. M (19 88) Genetic analysis and the construction of master strains for assignment of genes to six linkage...
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Is Education 1.0 Ready for Web 2.0 Students? docx
Ngày tải lên :
06/03/2014, 21:20
... University, it now has over 19 million registered users and ranks among the top 10 most visited Internet sites The success of MySpace, Facebook, and other popular networking sites such Friendster.com, ... students studied the capabilities of all these technologies—social bookmarking (or "distributed classification systems"), wikis, blogs, and other tools and employed them directly in their own distributed ... research projects (Mejias 20 06) Colleges are also using Web 2. 0 outside of the instructional context Campus administrators and police harvest information from online discussions and postings...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
Ngày tải lên :
30/03/2014, 11:20
... VRK2B (lower) isoforms The GST–p53 substrates used were FP2 21 , residues 1 85; FP279, residues 11 –65; FP267, residues 1 64 Fusion proteins were made with the murine p53, but the numbering is that ... VRK1 [11 14 ] and VRK2, although not for VRK3 [13 ] VRK1 has a nuclear localization signal and is detected in the nucleus in some cell lines and in transfected cells [11 ,13 ], but it is also present ... analysis was performed as previously described [16 ,17 ] 25 00 Acid hydrolysis and phosphoamino acid analysis An in vitro kinase assay was performed with GST–VRK2B and GST–p53 (FP267) as substrates The...
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Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx
Ngày tải lên :
30/03/2014, 15:20
... and is used by numerous GPCRs [11 ] The formation of the clathrin lattice is disrupted by hypertonic sucrose and this is an established method used to block Ó FEBS 20 04 internalization processes ... b-arr1–EGFP, as observed when coexpressed with the wild-type CRFR1 (Fig 5A3), is 15 0 A2 Intensity A1 10 0 50 Distance (µm) 15 0 B2 Intensity B1 25 10 0 50 0 Distance (µm) 20 Fig b-arrestin1 does not ... serine and threonine residues with the basic residues arginine or lysine at position )2, )3 [28 ] and four serine residues in this consensus sequence are present in the third intracellular loop and...
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Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
Ngày tải lên :
30/03/2014, 16:20
... C 2 12 12 Cdx -2 Cdx -2 Tub D 3 .1 Time (h) Tub 2. 9 1. 4 2. 7 2. 6 3.7 4.3 Caco -2 C C 2 4 6 12 12 24 24 Time (h) Cdx -2 Tub 0.9 0.9 1. 0 0.9 1. 0 Fig Forskolin ⁄ IBMX activates Cdx -2 promoter and Cdx -2 ... analysis The hamster pancreatic islet cell line InR1-G9, the mouse intestinal proglucagon producing endocrine cell lines GLUTag and STC -1, and the human colon cancer cell lines HT29, Caco -2 and SW480, ... endocrine cells In this study, we examined the effects of forskolin ⁄ IBMX on Cdx -2 expression, and investigated possible signaling pathways that mediate such expression We found that Cdx -2 expression...
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Báo cáo khoa học: Mitochondrial calcium signalling in cell death Delivered on 1 July 2004 at the 29th FEBS Congress in Warsaw pptx
Ngày tải lên :
30/03/2014, 20:20
... has been studied in relation to Ca2+ signalling is the state of fusion and fission The study of mitochondrial structure, and of the mechanisms that FEBS Journal 27 2 (20 05) 4 013 –4 022 ª 20 05 FEBS ... Bcl2 overexpression in HeLa cells [ 41] , are presented in A Fig 2A–C HeLa cells were transiently transfected with a cytomegalovirus-driven expression plasmid for Bcl -2 and Ca2+ homeostasis at the ... effects, e.g isoforms belonging to the same group, such as d and e, have been reported to play opposite effects on apoptosis To evaluate specific effects of PKC isoforms on cellular Ca2+ homeostasis,...
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Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury" ppt
Ngày tải lên :
18/06/2014, 19:20
... PEP -1- SOD1 and PEP -1- CAT fusion protein pET15b-SOD1-His, pET15b-PEP -1- SOD1-His, pET15bCAT-His and pET15b-PEP -1- CAT-His were successfully expressed and purified as shown in Figure 1A The results ... PEP -1- SOD1 and PEP -1- CAT Four prokaryotic expression plasmids with His-tag, pET15b-SOD1-His, pET15b-PEP -1- SOD1-His, pET15bCAT-His, and pET15b-PEP -1- CAT-His were constructed by the TA-cloning method The ... 3 .18 10 U/g, 3 .22 10 U/g, respectively These data suggest that fusion proteins PEP -1- SOD1-His or PEP-1CAT-His had the similar enzymatic activities as the wild type SOD1 or CAT Page of 11 almost...
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Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
Ngày tải lên :
18/06/2014, 22:20
... These findings are generally consistent with response profiles observed with other Aurora inhibitors [29 , 31, 33] and suggests these disease subtypes can serve as important predictors of response ... Genetics Analysis Figure Response profile of GSK1070 916 for hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are ... of mitosis, the relationship of Aurora expression and response to GSK1070 916 is still unclear In a subsequent analysis of the background genetics, we found NOTCH1 mutation status to be associated...
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