... separated to expose the zygoma and squamosal bone A burr hole of 1.5 × mm was made using a mm micro-drill rostal to the anterior junction ofthe zygoma and the squamosal bone The dura mater was ... Transplantation Seven days after the induction of focal cerebral ischemia, animals were anesthetized with an intra-peritoneal injection of 400 mg/kg chloral hydrate (Pharmaceutical Plant of Tiantan ... completion of behavioral assessments Tracking of BrdU+ NSC revealed that a majority ofthe donor cells engrafted to the infarcted areas ofthe cortex, hippocampus, striatum and parenchyma near the third...
... load and may increase the risk of HTLV-I-associated myelopathy The outcome of hepatitis B and papilloma virus infections has also been associated with HLA alleles The role of certain HLA antigens ... the spontaneous reappearance ofthe malignant melanoma also seemed to correlate with an ancestral SLA haplotype [22] In contrast, segregation analysis of melanocytic 124 P Chardon et al lesions ... sequence The sequencing ofthe class I region was essential forthe unravelling ofthe overall SLA class I organisation In particular, it permitted individual characterisation of all class I genes,...
... was calculated and plotted against physical distance LD was calculated using both the D′ coefficient (blue shaded area) as well as r2 (red shaded area) The averaged pairwise LD value across the ... single HLA-B partner (e.g HLA -A* 3303, HLAC*0302, HLA-DRB1*0301) Overall, there is a tighter linkage between HLA-C and HLA-B alleles as compared to HLA -A or HLA-DRB1 Each HLA-C allele has a dominant ... determined at each locus A number of HLA alleles stand out as being very common in the population, for example the HLA -A* 1101 allele is present at a frequency of 30% The most common allele at the HLA-B...
... had an average minor allele frequency of 8%, compared to an average of 4% forthe other 216 This result suggests that although the overall performance ofthe 1877-SNP map in capturing HapMap variation ... capture the variation ofthe 1877 SNPs in the Singaporean Chinese population The results are tabulated in Table 3.8 below Table 3.8 Transferability of HapMap tag SNPs HapMap Panel* Number of tag SNPs ... with the same set of criteria: selecting the minimal set of tags such that all test SNPs can be captured by a tag with an r2 or at least 0.8 Tag SNPs were also generated by treating the East Asian...
... and the haplotype that tags a HLA allele with the maximum r2 was taken as the tagging haplotype for that particular allele The results are shown in Table 3.9 Using single-SNP tags, most HLA alleles ... tags however perform much better than single tags 25 ofthe 26 HLA alleles were tagged with an r2 of at least 0.8, with a mean maximum r2 value of 0.95 In some cases the performance is dramatically ... HLA Alleles (See legend on the next page) A HLA-G - HLAA HLA-F Telo HLA -A A*3303 A* 0207 A* 1101 A* 2402 A* 0203 A* 0201 HLA-C B HLA-B LTA BAT2 C*0102 C*0801 Recombination Rate (cM/Mb) Extended Haplotype...
... and Class I regions, most probably reflecting the common lineage ofthe HLA -A0 2 haplotypes Although the DBB (A2 -B57-DR7) and MCF (A2 -B15-DR4) Caucasian haplotypes carry HLA -A0 2 alleles too, the ... Traherne et al 2006, Horton et al 2008) set out to completely sequence Caucasian CEHs, providing a wealth of variation data ofthe MHC in the Caucasian population The high-resolution SNP map of ... identify a consensus haplotype due to recombination (local CEHs) Large gaps are seen in ofthe Caucasian CEHs (APD, DBB, MANN, MCF and SSTO), as the sequence coverage ofthe MHC in these haplotypes...
... AAAAAAAAAAAAAAAAAAAAAAAA G G G G G G G G G G G G AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ... AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA C C C C C C C C C C C C C C C C C C C C AAAAAAAAAAAAAAAA ... TT AG AG AG AG AG AG AG AA AA AA AA AA AA AA AA AA AA AA AA AG AG AG AG AG AG AG GG GG GG GG GG GG GG GG GG GG GG GG AG AG AG AG AG AG AG AA AA AA AA AA AA AA AA AA AA AA AA AG AG AG AG AG AG AG...
... such as Graves’ disease, myasthenia gravis and psoriasis, as well as nasopharyngeal carcinoma (Chan et al 1978, Lu et al 1990, Chan et al 1993, Ren et al 1995, Vejbaesya et al 1998) The disease ... China, Singapore and Taiwan, it has a high frequency in indigenous populations in the Americas, Australia and Asia It is also found with a frequency of at least 5% in several Caucasian populations ... populations also reflect well the genealogy between the populations, with the Japanese population showing high correlation, followed by the Caucasians and the Africans The transferability of HapMap...
... Introduction 1.1 TheHumanMajorHistocompatibilityComplex (MHC) 1.1.1 Features oftheHuman MHC ThehumanMajorHistocompatibilityComplex (MHC) is a gene dense segment on the short arm ofthehuman chromosome ... ultimately spurious association signal for HLA -A3 in hereditary haemochromatosis Forthe majority ofthe MHC associated diseases, establishing a causative relationship between a disease and a MHC ... SLE and myasthenia gravis (Price et al 1999) Another example ofa disease associated CEHs in the Caucasian population is the A3 B7-DR15 haplotype that is associated with SLE and hereditary haemochromatosis...
... thank the Oklahoma Medical Research Imaging Core Facility for their assistance and Mrs Marilyn Bonham-Leyba for assistance with manuscript preparation References 10 Pramatarova A, Laganiere J, Roussel ... in large part by the ALS Association; the Oklahoma Center forthe Advancement of Science and Technology (HR02149RS); and the National Institutes of Health (AG20783, NS044154) We thank the Oklahoma ... immunosorbent assays (ELISAs; Cayman Chemical, San Diego CA USA) Nitrite assay Cell culture medium was assayed for NO2- by the Griess assay as described [8] Samples were mixed 1:1 with a mixture of equal...
... psychotherapy that, in turn, may repair the mechanism of crises, thereby making medication unnecessary Some authors explain the limitations of pharmacotherapy by the nature of BPD and complex ... guidance from the research staff about the criteria for each rating The nurses' judgment was based on review ofthe medical record, as a summary of all staff accounts Although the nurses assigned ... patients mislearn from their private experiments faster than they make progress in therapy with analysis ofthe transference and of scenarios of old betrayals The crisis intervention ofthe Cape...
... chronic phase In the chronic phase, there was a shift towards higher levels of macrophages in nasal and laryngeal cartilage than in the trachea The control mice had less than 5% macrophages T cells ... as the substrate and the amount of antibody was estimated as absorbency at 405 nm by using a Titertek Multiscan filter photometer All plates detecting the same antigen were analyzed at the same ... Arthritis Research & Therapy Vol No Hansson et al inflammatory attack ofthe nose and respiratory tract, phenomena that are commonly seen in RP patients There are also morphological similarities,...
... forthe « M system are obtained by addition ofthe values for M’ and M,, and we observe a lod-score value always greater than + 3.0 The estimation ofthe recombination rate between BoLA and the ... for which these animals were tested represent a powerful means of diagnosing incorrect parentage and this was not the case for any ofthe apparent recombinants Furthermore the fact that so many ... bull VALMIN Table gives the lod-score values obtained for M’ and forthe M, factor, and shows clearly the existence of genetic linkage between the BoLA complex and each of » the factors The Z values...
... attachment to CAR, a 46-kDa transmembrane protein that also serves as a receptor for many adenoviruses [17] In addition, some strains of CVB1, and can interact with an additional receptor, DAF, ... amounts of cells and viruses Statistical analyses Individual data pairs were analysed by the unpaired t test, and one-way analysis of variance followed by Dunnetts post-test was used to compare ... NJ drafted and wrote the manuscript MG developed the protocol forthe binding assay, radiolabeled and purified the clinical isolate and performed the radioactive measured binding assay SI participated...
... al, 1991; Pinard and Hepkema, 1992) A MHC genotype was defined as the combination of haplotypes Serological typing was performed on the parents ofthe 8th generation, on all the females and the ... males ofthe 8th generation, and on all the birds ofthe 9th and 10th generations Only the results of MHC typing in the 9th and 10th generations were used in the analysis Segregation ofthe haplotypes ... between haplotypes The estimated Ab titer ofthe 114 B-haplotype was significantly lower than the estimate ofthe 119 B-haplotype in the L line (table V) In the C line, the estimated Ab titer of the...
... ortholog of Caenorhabditis elegans (AhR-1C.E.), neither photoaf®nity labeled by a dioxin analog, nor activated by b-naphto¯avone in a yeast system [20]; the rainbow trout AhRa that binds TCDD [21] and ... to the bound state, Arg206 affects the position of Asp212, which in turn undergoes the largest conformational change of all the sidechains [12] Interestingly, although the conformation ofthe ... ofthe isolated domain PYP has been previously proposed as an appropriate structural template for AhR, but our analysis ofthe recently determined structures ofthe FixL and the HERG PAS domains...
... the Beginning, Intermediate and Advanced levels The backs ofthe Flash Facts are blank Teachers may use this area to translate the facts into the native language(s) ofthe student(s) or for additional ... away items rather than throwing these items into the waste stream Fora large number of unwanted items, students can hold a garage sale They can also be encouraged to shop at garage sales before ... in their native language Assign students to think of other ways to reduce the waste stream Ask “How can we reduce the waste stream?” Bring the class back together and have the pairs report their...
... ofthe translated RDQ High levels of internal consistency across the translated RDQ scales would be evidence ofthe amenability to translation Analysis revealed that, regardless of language, all ... structured by the trial case record form for each visit and graded = none, = mild, = moderate, and = severe Translation and cultural adaptation The RDQ was translated into Norwegian and Swedish according ... preferably selfadministered The multicenter, multinational nature of pharmaceutical clinical trials also requires questionnaires that are amenable to translation into multiple languages The Reflux...
... modifications References H Kabata, O Kurosawa, I Arai, M Washizu, S .A Margarson, R.E Glass, N Shimamoto, Science 262, 1561 (1993) Y Harada, T Funatsu, K Murakami, Y Nonoyama, A Ishihama, T Yanagida, ... external force In their setup, one end ofthe DNA was chemically attached to a surface and the other end to a magnetic bead held by the magnetic trap The DNA was forced to stretch upward by a hydrodynamic ... triphosphate (dNTP) to the growing chain of DNA for DNAP while RNAP catalyzes the addition of NTP to the growing chain of RNA; and (2) DNAP requires a primer (a short piece of ssDNA annealed to the...