... described in the Materials and methods. Completion ofthe hydrolysis wasconfirmed by the disappearance ofthe (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthattheformation ofthe N-terminal pyroglutamyl is not dependenton pH in the ... hydrogen bonded to the e-amino group ofthe Lys9 side-chain, which simultaneouslyinteracts with the main phosphate ofthe substrate [32]. The closer positioning ofthe N-terminus to the mainprotein...
... imagine the old knight, onlyhalf aware ofthe sunshine ofthe evening, the noise ofthe streets, the looks ofthe crowd, the great minsterrising half-finished in the midst ofthe town by the Rhine, ... Boiardo, ofthe Ferrarese court to which he belonged, and ofthe school of poetry (including the more notable but less original work of Ariosto) which the genius ofthe man and the character ofthe ... this: on the extreme pinnacle, high on the roof, they might indeed place against the russet brick or the blue sky, amid the hum of life and the movement ofthe air, the living man, like the Scaligers,...
... arboxylateoxygen of Asp273 (Asp366), the carbonyl oxygen ofthe P1residue with the side-chain amine of Lys262 (Lys354), and the backbone amino group ofthe P 1Â residue with the backbone carbonyl oxygen of ... identified the residues important for LAP catalysis and coordination of the two zinc io ns in the a ctive site. The i mpact of the mutations on the tomato LAP enzyme must be viewed in the context o f these ... waters and the bihydroxide ion in the general base mechanism of blLAP [21,24]. The recentidentification of bicarbonate in the active site ofthe nativeE. coli PepA and blLAP and the influence of bicarbonateon...
... fold, while the S15 contactsabove the internal loop of helix 22 and to the tetraloop of helix 23a stabilize the tertiary fold of the upper three-helix junction. The tertiarystructure ofthe upper ... regions ofthe central domainRNA are not involved in directing assembly of the domain but rather are displayed on the sur-face of a preassembled RNP core. Hence, for-mation ofthe core ofthe ... and S18. The structure ofthe complexdemonstrates the central role of S15-induced reorganization of central domainRNA for the subsequent steps of ribosome assembly. The recent explosion of structural...
... loop in the primed side ofthe binding sitegroove. Although the prodomains of cathepsins F andW share the same degree of similarity to the prodo-main of cruzipain, the similarity ofthe mature ... RGVDLQPS-LIS at the C-terminus, which resulted from the fusion of the propeptide with a small stretch of coding sequencewithin the multiple cloning site ofthe pQE30 plasmidbefore the stop codon. The predicted ... cross-inhibition of brucipain by PCZ. The pro-domain of cruzipain shares the highest identity with the C-terminal portion (residues Pro147–Leu251) of the prodomain of cathepsin F (32%) and that of cath-epsin...
... interaction in the presence of agonist R5020, but not in the presence of antagonistRU486 [54]. LxxLL motifs in the PR-B form were mostprobably not involved, because the shorter PR-A form,lacking these ... sequence of protein interaction events, includingN/C interaction, in regulation of AR function. Detailedknowledge on the role ofthe AR N/C interaction wouldrequire the elucidation of its function ... AR3)13deletion,whereas in the mammalian assay almost all reporter geneactivity was lost. The most obvious difference betweenboth assays is the coupling of AR.NTD to GalAD in the yeast assay, and the absence of a...
... FAD/NADPH domain(% 16-fold) (29). However, the domain mixture still recon-stituted only % 2% ofthe activity ofthe full length CPR. InthecaseofNR1,thekcatfor the full length enzyme is low(1.3 s)1) ... enzymes, the potentials for the FAD/NADPHdomains are rather more negative than those for the FMNdomain, reflecting the direction of electron transfer fromNADPH through FAD, then FMN and on to the ... BM3 [28]. Aggregation ofthe NR1FAD/NADPH domain was much less extensive in the absence of cofactor reduction.Stopped-flow kinetic studiesReduction ofthe FAD/NADPH domain of NR1 wasinvestigated...
... with the Substance P receptor [25]. Detachment of the cells from the surface ofthe culture dish could r eflectdisassembly ofthe actin cytoskeleton in the me mbraneregions as a consequence of ... both the cAMP and the PLC pathway [22], the second messengerproduct of either or both of these pathways couldbe invo lved in agonist-stimulated internalization of thisreceptor. The effect of ... f125I-labelled sauvagine could be observedin the presence of any of these inhibitors. These findingsindicate that neither the activation of PKA nor the activation of PKC is necessary for agonist-induced...
... determinant ofthe hydrophobic SÂ2 bindingpocket of trypsins is the side-chain of Tyr151 [18]. The importance of this residue is underlined by the fact that in the case ofthe second Kunitz-domain of ... raising the possibility that the inability of WFIKKN-KU2 to inhibitthese proteases is partly due to the lack of such a favorableinteraction ofthe PÂ2 Trp with the target enzymes. The factthat the ... (JASCO).Fig. 3. Alignment ofthe sequences ofthe second Kunitz-modules ofthe human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains ofthe human and the two fugu WFIKKNRP...
... abuse their office andinfluence to the injury ofthe laws and peace ofthe country. The elimination ofthe Christian teachers of the Negro race, and the prevention ofthe immigration of workers ... as their peculiar needs demanded. In the agricultural sections the first duty ofthe teacher ofthe blacks was to show them how to get their living from the soil. This was the final test of their ... resulting from the proximity of the communities, and to the coöperation ofthe Canadians. The previous experience of most of theseadventurers as sojourners in the free States developed in them such...
... reported, then a zero score was assigned. Per-formance scores were calculated by multiplying the fre-quency and severity scores, while the overall Child-OIDP score is the sum ofthe 8 performance scores ... shown by the identical Child-OIDP scores between the two administration modes.However, the different settings and disease profiles of the samples limit comparability of our results with the afore-mentioned ... Child-OIDP and some ofthe asso-ciations between the Child-OIDP and proxy measureswere not significant. While the low value of alpha isaffected by the nature ofthe Child-OIDP index and the fact that...
... per-formance, thescore may appear too high, and will reflect the actual function ofthe knee as well as the satisfaction of the patient. The Lysholm score has never been validatedfor the purpose of following ... in 55% ofthe patientsA (1–2 mm), in 29 % ofthe patients B (3–5 mm) and in16% ofthe patients C (6–10 mm) stability.Looking at the osteoarthritis rate ofthe operated knee, 55% ofthe patients ... osteoar-thritis of 1 grade in 52% ofthe patients, 35% of the patients had an increase of 2 grades and 3% ofthe patientshad an increase of 3 grades. (Table 4, 5 and 6) 10 % of the patients had...
... flexiondeformityofthehipof40degreesandfurtherflexionand rotations were painful. X-ray showed lytic lesion of the ilium and 2 centimetre proximal migration of the femoral head into the infected ... organs. Due to their physiologic role as capillaryfilters and their vast capillary volume, the liver and lungare most often affected. The brain, the muscle s or the bones are the more frequently ... recur-renceofthecystsdespitetheongoingAlbendazoletreatment. She returned again on 8/7/08, with increasingpain in the right hip. X-ray has shown that loosening of the acetabular component ofthe total...
... possible difficulty in intra-articular fracture ofthe distal radius is the displacement tendency of the radial styloid process due to the tension ofthe brachioradialis tendon.Methods: Ten patients ... drafting ofthe manuscript.GK participated in the design ofthe study and coordination and helped todraft the manuscript. All authors read and approved the final manuscript.Competing interestsThere ... in the interval between the flexor carpi radialis tendon and the radial artery. The distal part ofthe pronator quadratus muscle wasreleased from the radius. Care was taken to release onlythe...