the interaction of light with molecular electronic structure
Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
Ngày tải lên :
23/03/2014, 04:21
... interacting with the protein, because the resonance signals of the glucose residue overlapping with those of the galactose residue within the lactose affected the subtraction of the free induction ... to that of the higher binding site by NMR, even if the sugar-binding ability of one of the two binding sites is much higher than that of the other However, the hemagglutinating activity of lectin ... from the glucose residue in lactose were not observed However, the crystal structure of EW29Ch with lactose showed that the glucose residue of the lactose molecule interacted with subdomain c of...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
Ngày tải lên :
31/03/2014, 21:21
... characteristic of the monoformazan, i.e the fully reduced product of INT [15,17] In the following aeration of the medium, the absorbance of the spectral bands decreased, but the shape of the spectrum ... inward The rates of oxygen uptake were deduced from the slopes of the tangents to the oxygraphic traces, and the contact points of the tangents with the curves were used to determine the O2 concentrations ... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
Ngày tải lên :
20/02/2014, 02:21
... as a consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and ... the interactions of arginase II with l-arginine and agmatine are greatly altered by replacement of this residue with aspartate To the best of our knowledge, this is the first report in which the ... being involved in the stabilization of the transition state [27], but not in the stabilization of the substrate in the Michaelis–Menten complex [24] Also in agreement with this, the Km value was...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
Ngày tải lên :
16/03/2014, 23:20
... limited case of six triplet sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other individual ... produced by the ATT-template in the presence of DAPI (Fig 3, lane 4), the observation of a monophasic thermal transition is consistent with the prevalent presence of a single DNA structure in the complex, ... associated with the ability of the drug to bind and stabilize folded structures in ATT-triplet sequences, by favourable and selective interaction with the minor-groove of AÆT and TÆT base-pairs The molecular...
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Báo cáo Y học: Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins doc
Ngày tải lên :
17/03/2014, 10:20
... ratio of HDL to LPS is necessary for a saturation of the tumor necrosis factor-a production (Fig 10) The comparison of the results of the interaction of LPS with HDL to those published for the interaction ... bottom) From these data an approximation of the molecular shape of lipid A is possible: In the absence of HDL, it is conical with a higher cross-section of the hydrophobic than the hydrophilic ... types of examinations Preincubation of the cells with LPS Re (30 at 37 °C) and following addition of HDL, preincubation of the cells with HDL and subsequent addition of LPS Re, and incubation of the...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
Ngày tải lên :
23/03/2014, 03:20
... none of these motifs, although they are still capable of mediating vegetal transport [8–11] Therefore, the secondary structure of the LEs may as well be critical for RNA localization, as is the ... for the presence of known localization factors Input corresponds to 1% of the material used in the pulldown experiment The presence of the TAP-Vg1RBP band in the anti-Staufen blot is a result of ... anchoring to cortical actin upon arrival of the RNP at the vegetal pole of the oocyte In the context of these localizing RNPs, but also as integral part of the 42S storage particle, 42Sp50 might...
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Báo cáo khoa học: The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers potx
Ngày tải lên :
23/03/2014, 07:20
... by the particular disposition of key residues in the context of long peptides or even in the context of the full protein Bearing this in mind, a MPRP-S role in the interaction of the MPR with ... about the interaction of peptides with large unilamellar vesicles (LUV) The fluorescence quantum yield is dependent on the polarity of the microenvironment of the Trp residues, as well as on the ... Castanho The membrane interaction behaviour of MPRP-C and MPRP-I is independent of the membrane phase but not of the presence of charged lipids Cholesterol does not reduce the extent of or potentiate...
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Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf
Ngày tải lên :
23/03/2014, 11:20
... energetics The effect of temperature on the interaction of genistein with HSA has been followed in the range 17–47 °C The binding constant, K, exhibits a recipro- Fig Quantitation of the interaction of ... al Interaction of isoflavones with human serum albumin pocket interact with the carboxy group of fatty acids [24] Effect of genistein on tertiary and secondary structure of HSA The effect of increasing ... overlap of the emission spectrum of the donor with the absorption spectrum of the acceptor The energy transfer efficiency E is related to the distance (r0) between acceptor and donor, and also to the...
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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
Ngày tải lên :
23/03/2014, 13:20
... towards the 3¢ end of the top strand and the 5¢ end of the bottom strand (Fig 4B) This agrees with the presence of a larger residue that makes stable contacts with this region of DNA The interaction ... achieved The combination of these experiments with the footprinting results and previous 197 KNOX homeodomain–DNA interactions A B Fig A model for the interaction of HAKN1 with DNA (A) Diagram of the ... least one of the members of the CÆG pair at the fourth position of TGAC in the HAKN1–DNA complex The two leftmost positions of the core interact through the minor groove with the N-terminal arm in...
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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
Ngày tải lên :
30/03/2014, 01:20
... contacts with the interconnecting segments The structure of the PPIase module of SlyD closely resembles the structure of the PPIase domain of FEBS Journal 276 (2009) 45294544 ê 2009 The Authors ... cluster that is the core of the domain The IF domain of SlyD is aligned with the IF domain of the archaeal homologous MtFKBP17 [32] (Fig 3) The main difference between these two domains is the longer ... In the nal stages of this investigation, the solution structure of an N-terminal part of SlyD, SlyD(1165), was also determined [31] A comparison of these reports shows that the structure of the...
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Báo cáo Y học: Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes pdf
Ngày tải lên :
31/03/2014, 23:20
... random structure with only a small amount of helix In the presence of 50% TFE, the shape of the spectrum is that of an a helical structure, with the characteristic minima at 208 and 222 nm The percentage ... from the quenching of the intrinsic Trp uorescence intensity of the peptides, upon addition of SUVs The uorescence intensity at 350 nm, expressed as the percentage of the uorescence of the lipid-free ... decrease of the mean lifetime of the Trp residue(s) in the W56F- and WT- or W48F-penetratin The decrease of the mean Trp lifetime for the three peptides might account for the decrease of the Trp...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 06:22
... addition to these cytokines, growth factor signals trigger the release of IL-16 in RASFs, the present research from Smith and Cruikshank extends the panel of signals involved in the regulation of IL-16 ... studies as well as the SCID-mouse in vivo model of cartilage destruction Although a number of molecular pathways have been identified that contribute to the stable activation of RASFs, the precise cause ... research on the interaction of RA-IgG and RASFs as well as other recent data, however, may change the picture It has been reported by Huizinga and colleagues that in a cohort of patients with recent...
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Báo cáo y học: "HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromati" pptx
Ngày tải lên :
13/08/2014, 01:20
... [33] The surface of interaction of the IBD with a subset of these proteins partially overlaps with that of integrase (reviewed in [19]) The affinity of IBD for integrase is higher than for some of ... study and [7]) The enhancing effect of integrase on the chromatin binding strength of LEDGF/p75 could offer a molecular explanation for the conserved HIV-1 cofactor activity of the LEDGF/p75 ΔPWWP ... accumulate in the cytoplasmic compartment of the transfected mouse fibroblasts as a result of the saturation of the nuclear import pathway responsible for the nuclear localization of these mutants...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1
Ngày tải lên :
14/09/2015, 21:54
... is also related to the L- or D- enantiomer However the effect of enantiomer of the amino acid is dependent on whether the replacement affects the structure of the peptide and the amphipathicity ... role in the activity of antimicrobial peptides The one cysteine of the S3 peptide which is derived from the Sushi domain of Factor C allows the formation of S3 dimers The S3 dimer is capable of disrupting ... boat-like extended structure formed upon interaction with micelles The region which is rich in tryptophan in the middle of the peptides interacts with one layer of the membrane and the two termini...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 3
Ngày tải lên :
14/09/2015, 23:32
... value The presence of V4 further increased the compressibility of the monolayer, indicative of the increased molecular area, which was consistent with the result of excess surface pressure 154 The ... They have the same hydrophobic alkyl chains and differ in the headgroup The molecular packing of the monolayer is mainly dependent on the hydrophobic interaction between the alkyl chains of the ... disruption The direct observation of the kinetics of these processes would greatly enhance the understanding of the mechanism of V4membrane interactions In the future, a possible method to address these...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 2
Ngày tải lên :
14/09/2015, 23:32
... amplitude of the ACF is inversely proportional to the particle number N However, if the fluorescence yields of the two species are not the same, the fluorescence yield of the particles will affect the ... presence of melittin overlapped well with that of labeled POPG LUVs At a peptide/lipid ratio of 1:8, there was a slight increase in the amplitude of the ACF and in the diffusion time When the peptide/lipid ... overlapped with that of labeled DPPG LUVs When the peptide/lipid ratio increased to 1:20, the amplitude of the ACF rose and the diffusion time increased compared with DPPG vesicles The amplitude of the...
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Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain
Ngày tải lên :
03/10/2015, 20:32
... the dimer by the FPLC because of the large molecular weight (Figure 4.1.3) In further, these polymers may accelerate the process of the EEN aggregation Therefore preventing the formation of the ... Chapter presents the results obtained, while Chapter discusses the contribution of these results on understanding of the possible role of the random coil PRD and the influence of Ca2+ on EEN in ... amphipathic helix, thereby extending the helical backbone of the dimer at the tips Together with the BAR domain, this sequence motif is termed as N-BAR and can be found in a subgroup of the BAR-domain...
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Báo cáo y học: "Special considerations in the treatment of patients with bipolar disorder and medical co-morbidities"
Ngày tải lên :
25/10/2012, 10:51
... Therefore, psychiatrists must have a high index of suspicion for medical illness, as well as a basic knowledge of the risks associated with the use of medications in this patient population The ... deleted from the risk:benefit analysis In some cases a medication with greater side effects or medical risk may be the preferred treatment because of the documented efficacy of the agent either in ... internal organ involvement with onset of symptoms within to weeks of exposure The frequency of the syndrome is estimated at 10–100 per 100,000 persons exposed Risk of serious hypersensitivity...
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Manage the Explosion of Information with IBM Information Infrastructure
Ngày tải lên :
16/10/2013, 21:15
... meters, GPS systems & more Data Variety With the expansion of information comes large variances in the complexion of the available data – 80% of data is now unstructured, contributed largely by email, ... Planet with Dynamic Infrastructure Intro to Information Infrastructure What is IBM Information Infrastructure? The Power of Integrated Solutions and Industry Expertise IBM brings together a breadth ... 25TB of data into 1TB of storage capacity, 9x faster than any other solution in the market © 2009 IBM Corporation Building a Smarter Planet with Dynamic Infrastructure Intro to Information Infrastructure...
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