... treeswith the nt sequence ofthe open reading frame (ORF) of their cDNAs and the corresponding aa sequences, inTable 1. Summary ofthe purification of DNases I from the pancreases of three species of ... cloned the cDNA of each. A single amino acid(aa) substitution was confirmed to affect the thermalstabilities of vertebrate DNases I and, furthermore, one of the postulated mechanisms whereby thermal ... not. The same was true for these mutants ofthe rat and mouseDNase I (Fig. 3). These findings demonstrate that the nature ofthe amino acid at position 130 may generally andmarkedly affect the thermal...
... phần kĩ thuật này vào hệ thống canh tác. Kĩ thuật đáng lẽ được áp dụng từng phần, thay đổi tuỳ theo nhu cầu của nông dân và kĩ năng quản lý và hưởng lợi từ kĩ thuật đó. Một ví dụ tốt là kĩ ... nông dân không cắt tỉa cây vào những thời điểm mà các nhà nghiên cứu đã đưa ra, mà cắt lá cây theo lịch riêng của họ và cho dê ăn lá cây. Những cành dài thì được sử dụng để làm cột cho cây ... nông nghiệp và tài nguyên thiên nhiên và phát triển nông thôn. Từ năm 2003, Cơ sở môi trường to n cầu (GEF) đã sáng lập ra một chương trình tác nghiệp mới (OP 15) cho Quản lý đất đai bền...
... activation ofthe promoters, the distance between the CCAAT-boxes has to be 31 to 33 bp, probably to allow binding of the p300 co-activator. In G0and G1phases, transcription of thesecell ... Protein kinase, key regulator of cytokinesis, mediates attachement ofthe mitoticspindle tothe centromere, phosphorylates histone H3 during mitosisB-MYB ⁄ MYBL2 v-myb myeloblastosis viraloncogene ... promoters, mutation ofthe CHRleads to a more pronounced deregulation than the alteration ofthe CDE [40].Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein...
... operating at varioussteps in the cytotoxic activity ofthe drug, from adecrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance. Oftenseveral different ... ofthe tumor cells exhibiting IR;2, 25–50% ofthe tumor cells exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% ofthe tumor cellsexhibiting IR. The overall score was the ... SGC7901/PrPCwasdecreased to levels similar tothe controls because of their toxicity in cell death (P > 0.05). The effects of LY294002 on adriamycin accumula-tion and retention in SGC7901/PrPCcells were furtherdetermined...
... attempt to establish theimportanceof GSK3 in the acute regulation of glucose transport in terms of itsregulatory control of GS in muscle and fat cells we haveinvestigated the effects of Li and the ... and fat cells. To gain some insight intothis issue we subsequently monitored the effects of a number of inhibitors that target PI3K, the MAP kinase pathway,p38 MAP kinase and mTOR on Li-stimulated ... in glucose uptake would tend to negate thispossibility (Fig. 7B). To further investigate whether inactivation of GSK3 hasany regulatory input into the stimulation of glucose uptakeby insulin...
... a patch ofthe summer sky had dropped into it. The thatched roofs shone russet brown against the dark foliage ofthe hills. The temple roofs curved gracefully above the pink mist ofthe crepe ... knew the real meaning of that poor old worn -to- a-frazzle call ofthe East, for now the dominant note was the call of love. I heard it above the clink ofthe teacups. It was in the swish of ... petticoat. If I went tothe theater, church or concert, the call of that germ-ridden spot ofthe unholy name beat into my brain with the persistency of a tom-tom on a Chinese holiday. The Lady and...
... EosFP [36]. The crystal structure of lhemOFP isexpected to yield further insights into the chemical nat-ure of its red chromophore.With 3.5 ± 0.1 ns, the fluorescence lifetime of lhem-OFP is slightly ... been suggested to exert a photoprotec-tive function or to render the internal light spectrumfavorable for zooxanthellae photosynthesis [28–31].However, the nature ofthe photoprotective mechanismhas ... way tothe col-oration of M. cavernosa. The red fluorescent morphshowed high-level transcription ofthe green -to- red pho-toconvertible protein mcavRFP. In the green morph, the transcript of...
... amplification of their codingsequences. The GAPDH probe template was the EcoRI-HindIII fragment ofthe coding region [30]. To detect longtranscripts of cyclins A2 and B1, we amplified by PCR the 3ÂUTR of ... the FACS (data not shown).Northern blot analysis of mRNA from fractions of elutriated EL4 cellsWe needed to confirm the real-time PCR data by nor-thern blots of RNA from elutriated cells. The ... (Figs 4 and 5). The 1.8-kb tran-script of cyclin A2 that corresponds to 60–90% of the total cyclin A2 mRNA increased only about twofold,whereas the 3.0 kb cyclin A2 mRNA was 2.5- to 7-foldmore...
... InternalAffairsOfficeInspectionsandInvestigationsOfficeCivilRightsComplianceOfficeOffice ofthe DeputyDirectorforAdministrationOffice ofthe DeputyDirectorforCorrectionsOffice ofthe DeputyDirectorforLawEnforcementFiscalOfficeAdministrativeServicesOfficeTrainingandStaffDevelopmentOfficePersonnelManagementOfficeClassificationOfficeProgramCoordinationOfficeIntake Service CentersDivisionCommunityCorrectionalCentersDivisionCorrectionalFacilitiesDivisionHealthCareDivisionCorrectionsProgramServicesDivisionCorrectionalIndustriesDivisionProtectiveServicesDivisionExecutiveProtectionSectionNarcoticsEnforcementDivisionSheriffDivision*Administrativelyattached tothe department.This ... service fund on June 30, 2001 to its Annual InventoryThis is trial versionwww.adultpdf.com Office ofthe Auditor The missions ofthe Office ofthe Auditor are assigned by the Hawaii State Constitution(Article ... Audit of the Department of Public SafetyReport No. 02-10May 2002A Report to the Governorand the Legislature of the State of HawaiiConducted by The AuditorState of HawaiiandKPMG LLPTHE...
... seniority and once the ACO on the top of the list is called, that ACO moves tothe bottom ofthe list whether the ACO accepts, declines, or cannot be reached. The selection of individuals for ... inmates. The Offender Family Service Program Advisory Council consists of seven members appointed by the director that reviews and makesrecommendations tothe director to improve the types of servicesprovided ... services; and otherrelevant functions. The Office ofthe Deputy Director for Corrections provides for the custody, care, and assistance in the rehabilitation of all personsincarcerated by the...
... referred tothe stateDepartment ofthe Attorney General. Failure to notify the attorneygeneral on a timely basis negates the State’s ability to file a proof of claim for the salary overpayments. The ... intended to present the financial position, results of operations and cash flows of only that portion ofthe funds and accountgroups ofthe State of Hawaii that is attributable tothe transactions of ... materialrespects, the financial position ofthe Department of Public Safety, State of Hawaii, as of June 30, 2001, and the results of its operations and the cash flows of its proprietary fund type for the...
... consisted ofthe following as of June 30, 2001: The state Director of Finance is responsible for safekeeping of allmoneys paid into the State Treasury (cash pool). The state Director of Finance ... reflect the financialposition, results of operations, and cash flows ofthe following offices,divisions, and administratively attached agencies ofthe department:Office ofthe Deputy Director ... and the amount ofthe loss is reasonably estimable.Total Columns on the Combined Financial Statements The total columns are captioned Memorandum Only to indicate that theyare presented only to...
... for loss due tothe investment or failure of investment of funds and assets in the plan, but does have the duty of due care thatwould be required of an ordinary prudent investor. Therefore, inaccordance ... and is included in the department’s special revenue funds’expenditures. The department is exposed to various risks of loss related to torts; theft of, damage to, or destruction of assets; errors ... based on the employee’s age,years of credited service, and average final compensation (AFC). The AFC is the average salary earned during the five highest paid years of service, including the vacation...
... draft of this report tothe Department of Public Safety(department) on April 25, 2002. A copy ofthe transmittal letter to the department is included as Attachment 1. The response ofthe departmentis ... than 20 factors that causeovertime, and sick leave is just one of them. These factors can be grouped intwo major categories. The first group includes factors that impact on the number of employees ... included in the report, please submit them no later than Tuesday, April 30,2002. The Governor, and presiding officers ofthe two houses ofthe Legislature have also beenprovided copies of this...
... changed to require that we notify the unions ofthe overpayment. This adds an extra step in the process. If the employee and/or the union decide to dispute the overpayment, we must proceedthrough the ... untrue. Two ofthe three ACOs hadoutstanding leave records. The other did use 20 days during the year. However,ten ofthe twenty days were taken because of an extended illness. You thenmake ... each of which as mentioned earlier, has special staffing challenges. The Wardens are in touch with the unique security and program needs of theirfacilities. They are also in touch with their...