... oligo-merization domain (i.e. b sheets 7 and 8, the AAAdomain helix and the C-terminal helix). However, the majority of these proteins are likely to be other meioticclade AAA ATPases and have the AAA ... domain helixand the C-terminal helix, but not the b domain. The distinguishing feature of members ofthe meioticclade of AAA ATPases isthe SRH motif, which dif-fers from that of other AAA ATPases ... material is availableonline:Fig. S1. Sequence alignment ofthe C-terminal regions of Vps4, spastin, katanin and fidgetin from a range of species.This material is available as part ofthe online articlefrom...
... S. & Fire, A. (1998) RNA as a target of double-stranded RNA-mediated genetic interference in Cae-norhabditis elegans. Proc.NatlAcad.Sci.USA95, 15502–15507.44. Tabara, H., Grishok, A. & ... indicated by the open arrowhead, and the Ku-70 and -80 proteins are indicated by closedarrowheads. In addition, the asterisks represent reappearance ofthe WD proteins after 24 h (upper panel). The ... amplifi-cation was performed using LA Taq (Takara, Japan) in a total volume of 50 lL containing 55 pg of competitor. Atthis concentration, the band intensity ofthe amplifiedproduct from the competitor...
... phosphorylase, causes a large activation of glycogensynthase but a small stimulation of glycogen synthesis; (c)that the stimulation of glycogen synthesis by inactivation of phosphorylase is associated ... with both activation andtranslocation of glycogen synthase, and that the formermechanism alone cannot explain the stimulation of glyco-gen synthesis. This suggests that translocation of glycogensynthase ... ofthe phosphorylase inactivator, we suggest thatadditional factors may be involved in translocation of glycogen synthase and that phosphorylase a itself may be animportant determinant of the...
... some archaeologists as an indicationthat cremating people had come into Italy by sea, and that their migrationwas part ofthe larger upheaval which affected the eastern Mediterraneanat the ... integral aspect ofthe Villanovan Culture of northern and much of central Italy. Was the widespread practice of crema-tion during the Final Bronze Age a passing fad, so to speak, adopted andthen ... been able to arriveat many firm conclusions about the overall character of these languagesand their historical links to one another.1Before Latin began to drive the other languages of ancient...
... Tom is an international speaker and anadjunctprofessorin the Masters of TaxprogramatArizonaStateUniversity.Heservesas the RichDadAdvisorforTaxandWealthStrategyand isthe author of anupcomingRichDadAdvisorbookonbuildingwealththroughtaxstrategies. ... industry was born called nancial planners. Today it takes 30 days to become a nancial planner. It still takes a year and a half to become a massage therapist. RobertAndy,whatdoyouthink of mutualfunds?Andy ... my house had appreciated in value. Again, that’s capital gains versus cash ow. And what peoplearendingoutnowthat the realestatemarkethascrashedand the value of homeshasbeensuckedout…nowpeopleareupsidedownontheirhome,andthey’rendingoutit’s a liabilitybecausetheystillhavetopay the bankonthat...
... to adopt a black dog and wanted one a bit more mature as I was in an apartment. Lissa had been found, initially reunited with her family, and then they brought her back to the shelter a couple ... thinking about a small dog. Neither of our adult children have pets and perhaps they could take the dog while we are in South Africa. at night I was on the SPCA website for Northern Virginia. And ... eu-thanized isa “behavior problem”. Most behavior problems can be prevented by appropriate socialization and training, under the guidance ofa quali ed trainer. is takes a heavy investment of time,...
... evolutionary analysis of the DNase I family. The mammalian group formed a relatively tight cluster, while the snake (E. quadrivirgata,E. climacophora and A. blomhoffii), amphibian (X. laevis,Rana catesbeiana, ... quadrivirgata,E. climacophoraand A. blomhoffiiDNases ITotal RNA was isolated from each snake pancreas by the acid guanidinium thiocyanate/phenol/chloroform method[24] and any DNA contamination was ... reagent grade and available commercially. The snakes and Japanese white rabbits were acquired, main-tained and used in accordance with the Guidelines for the Care and Use of Laboratory Animals...
... annulata, Budonosoma granulifera, Cassiopeaxamachana, Condylactys gigantea, Gorgonia ventalina,Lebrunia danae, Palythoa caribaeorum, Physalia phy-salis, Plexaura homomalla, Stichodactyla helianthus ... magnifica Phylum Annelida, Class Polychaeta, SubclassPalpata, Order Canalipalpata, Suborder Sabellida, Family Sabellidae,Genus Sabellastarte [14] The ‘tentacle crown’ and the ‘body’ parts of ... the kingdom Methazoa(Phyla: Annelida, Urochordata, Echinodermata, Cnidaria,Mollusca, Artropoda) were collected in the north coast of Havana and classified by Cuban specialists at the NationalInstitute...
... reason for this phenomenon (namely enzymeinstability) is discussed in greater detail below.Instability ofthe mutant enzymeFurther analysis ofthe kinetic parameters ofthe G167Emutant was ... destabilization ofthe ISP [14].It seems probable that this is also the case for the G167Emutant, and that activity is lost owing to the destabilization of the complex (loss ofthe ISP) on dilution ... mitochondria. Studies ofthe mechanism of assembly of the bc1complex suggest that the ISP is one ofthe lastsubunits to be integrated within the membrane-boundsubcomplex [2]. The integration of the...
... proteins in the marine Califor-nia sea slug (Aplysia californica) and in the freshwatersnail Biomphalaria glabrata. These CRD homologswere extracted from ESTs from the Aplysia ESTproject and the ... 5)appeared to migrate normally. To study whether the abnormal migration ofthe mature forms of R19 4A- PCSK9 and D20 4A- PCSK9 was due to altered auto-catalytic cleavage, western blot analyses of ... two mutants were autocat-alytically cleaved in a normal fashion.To study whether the abnormal migration of mature, cleaved R19 4A- PCSK9 and D20 4A- PCSK9was due to abnormal glycosylation, the sensitivities...
... UNEGOISTIC ACTSA21 // On the Genealogy of Morals, First Essay Castillo / Saw / Paris / Tan / Medina, K BEGAN AFTERUTILITARIANISMMASTER MORALITYSA21 // On the Genealogy of Morals, First Essay ... WAR BETWEEN THE RICH AND THE POOR.SO THE GOOD-CONTRARY POLARITY BECAME A. K .A. THE MASTERS AND THE SLAVES.SA21 // On the Genealogy of Morals, First Essay Castillo / Saw / Paris / Tan / Medina, ... Morals, First Essay Castillo / Saw / Paris / Tan / Medina, KAS A VITAL SOCIAL COMPONENT, LANGUAGE ADAPTS TO ITS USERS.SA21 // On the Genealogy of Morals, First Essay Castillo / Saw / Paris...
... Mariolis A, Mihas C, Alevizos A, Papathanasiou M, Mariolis-Sapsakos T, Marayiannis K, Koutsilieris M: Evaluation ofa clinical attachment in Primary Health Care as a component of undergraduate ... continuous variables. The choice of meth-ods for statistical testing of continuous variables wasbased on whether the data permitted parametric or non-parametric analysis. Categorical variables were ... interests.Authors' contributionsPM and NCK conceived and coordinated the study and drafted the paper; PMcarried out the mathematical analysis; AT, DK, NS, NP and ET collected data andassisted...
... transplantation produce quality of lifebenefits? A quantitative analysis of literature. Transplantation1997, 64:1261-1273.15. Fujisawa M, Ichikawa Y, Yoshiya K, Isotani S, Higuchi A, Nagano ... consist-encies.Construct validity- Factor analysis The factor analysis with varimax rotation identified a structure of five factors, which accounts for 46.3% of the total variance (Table 4). The content of each dimensionwas ... interpreted the data,drafted the manuscript; EJ participated in the design of the study, collected the data and performed the statisticalanalysis BD and VM: participated in the design of the study,...