the circle class contains one property called radius it will be defined when you instantiate an object and pass the parameter whatradius through the constructor function
macromedia flash mx advanced for windows and macintosh
Ngày tải lên :
03/06/2014, 02:04
... symbol-editing mode to the Timeline, and how to manipulate layers and frames You should also be familiar with importing and using bitmaps and sounds, and assigning basic actions to frames and buttons ... so you should already be comfortable with the basic tools and commands for creating simple Flash movies You should know how to create and modify shapes and text with the drawing tools and be ... Notice the breakups between the s and the p, and the hole that appears between the h and the e As is the case with motion tweening, simplifying a complicated shape tween into more basic parts and...
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This questionnaire is designed to investigate teachers’ attitude towards the applicability of process approach in teaching writi
Ngày tải lên :
07/09/2013, 13:51
... book, the teachers and learners’ profile and their methods and strategies in teaching and learning writing skill On the whole, the teaching and writing at non- major class is not as effective and ... time and hand in the composition for the teacher to correct In other words, in the process approach the focus of 10 teaching and learning is placed on the process of writing rather than the final ... emphasis on the process of writing All writers make decisions on how to begin and how to organize the task Therefore, they not write on a given topic in a restricted time and hand in the composition...
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Tài liệu In this lab, 2 ISDN routers are required. If ISDN routers are not available, review the lab to become familiar with the process. An Adtran Atlas550 ISDN emulator is used to simulate the switch/ISDN cloud. pdf
Ngày tải lên :
21/12/2013, 19:15
... associate the Dialer Profiles with the Dialer Interfaces that will be used, when needed Create a Dialer Pool, and put the interfaces and the associated Dialer Profiles in a common pool The commands ... Define switch type and spid numbers To configure the switch type and spid numbers use the following commands Router(config)#hostname Tokyo Tokyo(config)#enable secret class Tokyo(config)#isdn switch-type ... Type n and then Enter The responding line prompt will be: Proceed with reload? [confirm] Press Enter to confirm In the first line of the response will be: Reload requested by console After the router...
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Tài liệu Báo cáo khoa học: The PA-TM-RING protein RING finger protein 13 is an endosomal integral membrane E3 ubiquitin ligase whose RING finger domain is released to the cytoplasm by proteolysis ppt
Ngày tải lên :
18/02/2014, 08:20
... 4B, lanes and 5) As these proteins are also recognized by the anti-FLAG serum, they must possess both residues 38 and 381 and therefore be close to full-length RNF13 The lower molecular mass bands ... detected with rabbit anti-FLAG serum and, as secondary antibody, anti-rabbit Texas Red serum Cells were counterstained with mouse antibodies raised against PDI (E) and donkey anti-rabbit Alexa ... brain The solubilization of the ICD of RNF13 and the presence of the putative NLS on the C-terminal side of the TM domain are consistent with the possibility that the ICD, released from the membrane...
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Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
Ngày tải lên :
19/02/2014, 17:20
... 66 and 46 kDa (C) Cells expressing sTNSALP were pulse-labeled with [35S]methionine ⁄ cysteine for 30 and chased for h (lane 1), for 0.5 h (lanes and 5), for h (lanes and 6) or h (lanes and 7) The ... lM LLM (lanes and 4), 50 lM LLnL (lanes and 6) or 50 lM MG-132 (lanes and 8) The cell lysates were subjected to immunoprecipitation and the immune complexes were analysed by SDS ⁄ PAGE and fluorography ... TNSALP (lanes 1–4), sTNSALP (lanes and 6) or TNSALP (1559delT) (lanes and 8) were labeled with [35S]methionine ⁄ cysteine for h The cells were lysed in buffer containing Triton X-114 and partitioned...
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The nobel prize in physiology or medicine 2010 is awarded to
Ngày tải lên :
23/02/2014, 20:45
... Secretary of the Nobel Assembly; Outi Hovatta, Obstetrics and Gynaecology; Christer Höög, Genetics; Klas Kärre, Immunology, Chairman of the Nobel Committee; Hugo Lagercrantz, Paediatrics; Urban Lendahl, ... Clinic, Robert Edwards giáo sư danh dự Đại học Cambridge Ban biên tập năm giới thiệu phổ biến giải Nobel Sinh lý Y khoa bao gồm cố vấn khoa học sau đây, tất giáo sư Viện Karolinska: Göran K Hansson, ... R.E Người đoạt giải Robert G Edwards Robert G Edwards sinh vào năm 1925 Batley, Yorkshire, Anh Đối với hầu hết nghiệp học tập sinh lý sinh sản, ông làm việc Cambridge, Anh, nơi ông đồng nghiệp...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
Ngày tải lên :
07/03/2014, 09:20
... numbers of strands in their core structure HsSTP contains an additional b strand at the N-terminus extending the b1 sheet by one strand (Fig 1) TxSTP also contains a similar, albeit much shorter, ... red The three active site metal ions (M1, M2 and M3) are shown as gray spheres The N- and C-termini, and the two b sheets are labeled (B) The structure has been rotated through 90 ° around the ... coordinated by Asp118, Asp192 and four water molecules (Fig 2A) Asp192 thus bridges metals M1 and M3 The position of M3 between the flap subdomain and the active site is analogous to the third metal, Mn2+,...
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Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf
Ngày tải lên :
08/03/2014, 08:20
... immunoprecipitated as described above Beads were washed three times with immunoprecipitation buffer containing Triton X-100 and twice with immunoprecipitation buffer without Triton X-100 and proteins ... restored These results therefore show that the extent of glycosylation of GC-C determines its ability to be ligand-stimulated, and responsiveness of cells to the ST peptide can be controlled by the ... of the Bradford protein assay increases its sensitivity: theoretical and experimental studies Anal Biochem 236, 302–308 30 Visweswariah, S.S., Ramachandran, V., Ramamohan, S., Das, G & Ramachandran,...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
Ngày tải lên :
15/03/2014, 00:20
... Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14, ... presence of Ssa1p (lanes 4, 7, 10 and 13) were treated with BS2G (lanes 2–7) or BS3 (lanes 8–13), in the presence of ADP 0.5 mM (lanes 2–4 and 8–10) or mM ATP and mM MgCl2 (lanes 5–7 and 11–13) (B) ... stained with antibodies directed against Ure2p or His-Tagged Ssa1p (B) (A) A mixture of untreated Ure2p and Ssa1p (lane 1); Ure2p alone (lanes 2, 5, and 11), Ssa1p alone (lanes 3, 6, and 12), and...
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Economic Effects of Reducing the Fiscal Restraint That Is Scheduled to Occur in 2013 docx
Ngày tải lên :
15/03/2014, 20:20
... 2013, when withholding schedules will reflect those expirations Altogether, those changes will reduce the deficit by $221 billion between fiscal year 2012 and 2013 The Middle Class Tax Relief and ... that extended the lower tax rates and expanded credits and deductions originally enacted in the Economic Growth and Tax Relief Reconciliation Act of 2001 (P.L 107-16), the Jobs and Growth Tax ... will have a much larger impact on the economy in 2013 The increases in taxes and decreases in government benefits will lead households to cut back their purchases of goods and services, and the...
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Báo cáo khoa học: MPP3 is recruited to the MPP5 protein scaffold at the retinal outer limiting membrane ppt
Ngày tải lên :
16/03/2014, 13:20
... regions and together with DLG1 and MPP5 comprises a HOOK domain situated between SH3 and GuK domains Table MPP3 was individually aligned with MPP4, MPP5, DLG1 and Stardust (STD) The identities and ... link to channels or receptors, therefore retinal MPP3 and ⁄ or MPP4 may be involved in channel or receptor positioning, stability at the membrane and its function The colocalization and interaction ... monoclonal anti-Dlg1, with normal mouse IgGs as control The membranes were probed with anti-MPP3 and a positive signal was observed only in the lane of Dlg1 immunoprecipitation and not in the control...
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how the use of the diary form narrative is beneficial to the
Ngày tải lên :
21/03/2014, 22:04
... and the reader can feel as if they are part of the group and read, and think along with the characters Another thing dealing with the diary form is quick referencing The reader, if needed, can ... exactly how a person was talking and acting through the written dialects In the novel Dracula, all the diaries, of all the individuals come together and in the end, become one powerful, combination ... possible because if one person was telling the whole story, the reader would see and hear what the person telling the story heard and wrote down So using, the diary, Bram Stoker could make the reader...
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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot
Ngày tải lên :
23/03/2014, 06:20
... stressful conditions, and the cortical cytoskeleton is therefore disrupted and hDlg released into the cytoplasm The lack of effect of an hDlg mutant, which cannot be proteolysed at initial apoptosis, ... phosphorylation sites were the four sites regulated by stresses (S158, T209, S431 and S442), one site constitutively phosphorylated in cells (S122) and one site phosphorylated in vitro by p38c (S447) ... cytosol, nucleus and membrane [3] Under these conditions, hDlg may be more accessible to the kinase and this may facilitate its phosphorylation after sorbitol treatment In addition, we have also...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
Ngày tải lên :
23/03/2014, 07:20
... than 0.7 (B) Immunoblot detection of PCaP1 orthologous protein in crude membrane fractions with anti-PCaP1 Lanes and 6, A thaliana; lanes and 7, Raphanus sativus; lanes and 8, Brassica rapa; lanes ... lanes and 9, B rapa var glabra; lanes and 10, B oleracea var italica The amount of protein applied was lg for A thaliana and 40 lg for the other plants 43 kDa) and Brassica oleracea var italica ... associated with the plasma and organelle membranes via covalent modification with lipids, formation of complexes with membrane integral proteins and association with membrane components such as membrane...
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Báo cáo khoa học: The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH docx
Ngày tải lên :
23/03/2014, 21:20
... where F and F ° are the fluorescence intensities in the presence and in the absence of the quencher, Q, respectively, and KD the Stern–Volmer constant [34] The constant, KD, depends upon the lifetime ... between the G2 calcium binding site and the G6 site through the interface Low pH-induced conformational changes within G2 possibly reflect altered association with G1 and/ or G3, rather than G6 as ... further shown that residues 203–225 and 159–193 within G2 form the G6 binding site, and that occupancy of a calcium binding site in G2 induces conformational changes through this interface with...
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She is going to go on business at the end of June potx
Ngày tải lên :
02/04/2014, 21:21
... gian) Ví dụ: at the end of the street (cuối đường), at the end of the book (cuối sách) Mạo từ the đứng trước từ bắt đầu nguyên âm “a, e, i, o, u”, the phát âm /ði/ - Trái nghĩa với “at the ... “at the beginning” – bắt đầu Ví dụ “at the beginning of May” – vào đầu tháng Năm - Cần ý phân biệt "at the end of " "In the end" để tránh nhầm lẫn “in the end” = “finally” – cuối cùng, sau “in the ... chủ ngữ “she” nên động từ “to be chia “is” -“go on business” – công tác “go” động từ nguyên thể sau cụm “ is going to” - “at the end of June” – vào cuối tháng “at the end (of something)” – vào...
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Báo cáo sinh học: "Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFb1-signaling and increased Galectin-3 expression: A histological study on biopsies" pot
Ngày tải lên :
18/06/2014, 19:20
... Erlangen), initiated and conducted the study, formulated the hypothesis, established and conducted the methods and analytic procedures, and wrote the manuscript PH formulated the hypothesis and ... are expressed as the median, the interquartile range (IQR), standard deviation (SD), and range Box plot diagrams represent the median, the interquartile range, minimum (Min), and maximum (Max) ... Therefore, the roles of TGFb1 and Galectin-3 in cellular differentiation, tissue regeneration, and inflammation may be relevant to the mechanisms underlying BRONJ The American Society for Bone and Mineral...
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Báo cáo sinh học: " Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" pot
Ngày tải lên :
18/06/2014, 22:20
... in the design of the study and drafted the manuscript Both authors read and approved the final manuscript Acknowledgements 18 19 The authors thank Prof Åke Lundkvist for fruitful discussion and ... functionally competent but less competitive than TULV02 One reason for the observed lower fitness of the recTULV might be that it was generated in the presence of the wt variant, with which it ... recombinant S RNA segments Checking of specificity of RT-PCRs for the wt and the Checking of specificity of RT-PCRs for the wt and the recombinant S RNA segments Lines 1–3: products of RT-PCR with...
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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
Ngày tải lên :
20/06/2014, 01:20
... to the conception of the studies and the critical review of the manuscript PCT contributed to the design of experiments, the acquisition and analysis of data and the critical review of the manuscript ... contributed to the conception of the studies and the critical review of the manuscript DCQ contributed to the design of the experiments and analysis of the data KAK contributed to the acquisition and interpretation ... of the mutant virus to some antiviral drugs A set of thymidine analogs was selected and a standard plaque reduction assay was used to evaluate the susceptibility of the dUTPase mutant and the...
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Báo cáo hóa học: " Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infection" pdf
Ngày tải lên :
20/06/2014, 01:20
... JMD carried out the experiments and wrote the manuscript BRM and PLC provided advice and wrote the manuscript AB conceived the study, carried out the experiments and wrote the manuscript All authors ... common site of entry and replication for various pathogens, the design of more effective vaccines and therapeutics will be greatly aided by gaining a better understanding of the local mechanisms ... infected by the IN route, the following titers (log10 PFU per g of lung tissue) were detected in the two animals euthanized on each day: day 2, 2.9 and
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