... demonstrates theimportantroleof GALA in the degradation of both linear andL-arabinose-substitutedgalactan side chains of pectin. This is in agreement with aprevious study in which the synergy of ... mainchain cleaving enzymes as well as with the arabinanolyticgenes [1]. The absence of expression of galA inthe presence of pectic galactan indicates that galactose is not an inducer of galA ... presence of D-xyloseand xylan as well. This can be explained by the structure of the polysaccharides. Xylan contains single b-1,4-linkedD-galactose residues but no b-1,4-linked galactan chains.Therefore...
... thio-b-D-galactoside; lane 5,purified NtKTI1 is indicated by an arrow inthe right lane. (B) In vitrobovine trypsin inhibition by the recombinant NtKTI1 protein (opencircles). Soybean trypsin inhibitor (triangles) ... R (1991) Cloning and sequencing of the cDNA encoding the major albumin of Theobro-ma cacao. Identification ofthe protein as a member of the Kunitz protease inhibitor family. Planta 183,528–535.27 ... sub-categorized into several families, including serine, cys-teine, aspartic and metalloproteinase inhibitors [11].Serine proteinases appears to be the largest family of proteinases, and plant serine PIs...
... oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds. The interactions should maintain the structure ofthe C-terminalregion. Inthe ... region on the opposite side of the N-terminal region on the molecular surface. Inthe wild-type enzyme, the side chain of Asp266 interacted with the amino group ofthe main chain at Glu268. The oxygen ... alkalinity than N263D-Sendai. The mutations at these positions in Sendai should promoteinstability ofthe surface region. As the C region of Sendaialso would play animportantrolein restraining...
... histidine residue (Eqn 4).Similarly, Shaanan [31] reported the stereochemistry of the iron-dioxygen bond in human HbO2by single-crystalX-ray analysis. Inthe a chain, the distance between Ne of His ... oxygen binding, so thatthese are named the sliding contacts. At the a1b1anda2b2interfaces, on the other hand, negligible changes are foundinsofar as the crystal structure was examined. Consequently,these ... residues [21±25]. Therefore, the twotypes ofthe ab contact a re deđned inthe molecule. One is the a1b1(ora2b2) contact involving B, G, and H helicesand the GH corner, and other is the a1b2(ora2b1)...
... ofthe AG/A site abrogated the ability of the transfected A12L gene to rescue, indicating that A12L proteolysis playsanimportantrolein viralreplication. Electron microscopy analysis ofthe ... tetracycline. Inthe absence of tetracycline, replication of vvtetOA12L wasinhibited by 80% and this inhibition could be overcome by transient expression ofthe wild-typecopy ofthe A12L gene. In ... These morphogenic defects suggested that A10Lprotein is required for the correct organization of the nucleocomplex within the IVs [7,8]. L4R, a DNA bindingprotein, playsan essential role in...
... along the Brenta. It is the month of May, and the meadows are in flower. In the horizon she sees the snowy peaks ofthe Tyrolese Alps standing out. The remembrance ofthe long hoursspent at the invalid's ... question is the object of our study. Inthe solution of it lies the interest, the drama and the lesson of George Sand's destiny.IIBARONNE DUDEVANT MARRIAGE AND FREEDOM THE ARRIVAL IN PARIS ... feverishly anxious to destroy everything, in order to create all things anew. In everything, in art, ideas and even in costume, there was the same explosion of indiscipline, the same triumph of capriciousness....
... crucial in adipogenesis, and that it plays important roles in events early inthe differentia-tion process, including MCE and the induction of C ⁄ EBPb and C ⁄ EBPd genes. Taken together, theseresults ... domainsplay important roles inthe function of proteins contain-ing this domain [40]. In particular, p47phox, which con-tains the PX domain and two SH3 domains, has beenintensely studied. In ... binding ofthe FAD49 PXdomain to phosphoinositides might be regulated by the interaction between the PX domain and the third SH3domain, as in p47phox, although whether the interaction of the...
... observed in HIV-1 IN. Thus, the number of amino acids forming the linker in ASV IN is muchsmaller than in HIV-1 IN, although the distancebetween the start and end points of these linkers is ... insertion into the chromosome ofthe host cell. The proteinconsists of three domains, the central catalytic core domain flanked by the N-terminal and C-terminal domains, the latter being involved in ... and may be in uencedby other parts ofthe molecular complex.Interdomain contactsOne ofthe measures ofthe extent of interactionsbetween the domains ofIN (dimerization of identicaldomains,...
... lethal dose of endotoxin [28]. To assess whether an acyltransferase inhi-bitor affects transcription or the processing and release of the TNF-a protein, MonoMac-6 cells were preincubatedwith ... strongly suggesting thatLPCAT playsanimportantrolein mediating the signalingpathways for LPS-activation of these cells.AcknowledgmentsWe are grateful to the Yamanouchi Research Institute, ... (1998) The role of lysophosphatide acyltransferases and protein kinase C isoforms in the regulation of lymphocyte responses. Biochem. Soc. Trans.26, 370–374.15. Jackson, S.K. (1997) Therole of...
... conclude, in doing an FTA, the speaker needs to balance three wants:- The want to communicate content ofthe FTA- The want to be efficient (or urgent)- The want to maintain H’s face to any degree1.3.2.3. ... deliberations of the phenomenon. Addressing hedging, it can be defined plainly as theprocess whereby the author reduces the strength of what he is writing”. Markkanen and Schroder (1985) define hedging ... strategy of “saying less than one means”, the function of strategy being to modify the writer’s responsibility for the truthfulness ofan utterance, to modify the attitude ofthe author to the propositions...
... targeting, we divided it into three tri-glycine segments andexamined the importance of each domain in targeting specificity in vitro.Replacement ofthe most C-terminal segment with alanine residues ... resulted in mistargeting the protein to the stroma, while exchange of either of the other two tri-glycine regions had no effect on correct targeting. Further-more, simultaneous replacement ofthe ... N-terminal and middle tri-glycinesegments with alanine repeats did not cause mistargeting ofthe protein asmuch as those ofthe N- and C-terminal, or the middle and C-terminal seg-ments. These...
... events at the NBDs. IfTM12 is involved inthe coupling process between the Fig. 2. Analysis of probe labelling of mutant TM12 isoforms of ABCB1. For each ofthe mutant isoforms, densitometric analysiswas ... func-tion is the molecular detail oftheprocessof couplingbetween the NBDs and TMDs. The most striking evi-dence for the presence of coupling between the twodomains is the ability of transported ... labelling is expressedas the fraction of specific labelling of single-cysteine isoforms over the specific labelling ofthe G324C positive control. Values represent the means ± standard errors of the...