... demonstrates theimportantroleof GALA in
the degradation of both linear and
L
-arabinose-substituted
galactan side chains of pectin. This is in agreement with a
previous study in which the synergy of ... main
chain cleaving enzymes as well as with the arabinanolytic
genes [1]. The absence of expression of galA inthe presence
of pectic galactan indicates that galactose is not an inducer
of galA ... presence of
D
-xylose
and xylan as well. This can be explained by the structure of
the polysaccharides. Xylan contains single b-1,4-linked
D
-galactose residues but no b-1,4-linked galactan chains.
Therefore...
... thio-b-
D-galactoside; lane 5,
purified NtKTI1 is indicated by an arrow inthe right lane. (B) In vitro
bovine trypsin inhibition by the recombinant NtKTI1 protein (open
circles). Soybean trypsin inhibitor (triangles) ... R (1991) Cloning and sequencing
of the cDNA encoding the major albumin of Theobro-
ma cacao. Identification ofthe protein as a member of
the Kunitz protease inhibitor family. Planta 183,
528–535.
27 ... sub-
categorized into several families, including serine, cys-
teine, aspartic and metalloproteinase inhibitors [11].
Serine proteinases appears to be the largest family of
proteinases, and plant serine PIs...
... oxygen of
the main chain at Asp266 was bound to amino groups of
main chain of Tyr269 and Ala270 by hydrogen bonds. The
interactions should maintain the structure ofthe C-terminal
region. Inthe ... region on the opposite side of
the N-terminal region on the molecular surface. Inthe wild-
type enzyme, the side chain of Asp266 interacted with the
amino group ofthe main chain at Glu268. The oxygen ... alkalinity than N263D-Sendai.
The mutations at these positions in Sendai should promote
instability ofthe surface region. As the C region of Sendai
also would play animportantrolein restraining...
... histidine residue (Eqn 4).
Similarly, Shaanan [31] reported the stereochemistry of
the iron-dioxygen bond in human HbO
2
by single-crystal
X-ray analysis. Inthe a chain, the distance between N
e
of
His ... oxygen binding, so that
these are named the sliding contacts. At the a1b1anda2b2
interfaces, on the other hand, negligible changes are found
insofar as the crystal structure was examined. Consequently,
these ... residues [21±25]. Therefore, the two
types ofthe ab contact a re deđned inthe molecule. One is
the a1b1(ora2b2) contact involving B, G, and H helices
and the GH corner, and other is the a1b2(ora2b1)...
... ofthe AG/A site abrogated the ability of the
transfected A12L gene to rescue, indicating that A12L proteolysis playsanimportantrolein viral
replication. Electron microscopy analysis ofthe ... tetracycline. Inthe absence of tetracycline, replication of vvtetOA12L was
inhibited by 80% and this inhibition could be overcome by transient expression ofthe wild-type
copy ofthe A12L gene. In ... These morphogenic defects suggested that A10L
protein is required for the correct organization of the
nucleocomplex within the IVs [7,8]. L4R, a DNA binding
protein, playsan essential role in...
... along the Brenta. It is the month of May, and the meadows are in flower. In
the horizon she sees the snowy peaks ofthe Tyrolese Alps standing out. The remembrance ofthe long hours
spent at the invalid's ... question is the object of our study. Inthe solution of it lies
the interest, the drama and the lesson of George Sand's destiny.
II
BARONNE DUDEVANT MARRIAGE AND FREEDOM THE ARRIVAL IN PARIS ... feverishly anxious to destroy everything, in order to create all things anew. In everything, in
art, ideas and even in costume, there was the same explosion of indiscipline, the same triumph of
capriciousness....
... crucial in adipogenesis, and that it
plays important roles in events early inthe differentia-
tion process, including MCE and the induction of
C ⁄ EBPb and C ⁄ EBPd genes. Taken together, these
results ... domains
play important roles inthe function of proteins contain-
ing this domain [40]. In particular, p47
phox
, which con-
tains the PX domain and two SH3 domains, has been
intensely studied. In ... binding ofthe FAD49 PX
domain to phosphoinositides might be regulated by the
interaction between the PX domain and the third SH3
domain, as in p47
phox
, although whether the interaction
of the...
... observed in HIV-1 IN. Thus, the number of
amino acids forming the linker in ASV IN is much
smaller than in HIV-1 IN, although the distance
between the start and end points of these linkers is ... insertion into the chromosome ofthe host cell. The protein
consists of three domains, the central catalytic core domain flanked by the
N-terminal and C-terminal domains, the latter being involved in ... and may be in uenced
by other parts ofthe molecular complex.
Interdomain contacts
One ofthe measures ofthe extent of interactions
between the domains ofIN (dimerization of identical
domains,...
... lethal dose of
endotoxin [28]. To assess whether an acyltransferase inhi-
bitor affects transcription or the processing and release of
the TNF-a protein, MonoMac-6 cells were preincubated
with ... strongly suggesting that
LPCAT playsanimportantrolein mediating the signaling
pathways for LPS-activation of these cells.
Acknowledgments
We are grateful to the Yamanouchi Research Institute, ... (1998) The role
of lysophosphatide acyltransferases and protein kinase C isoforms
in the regulation of lymphocyte responses. Biochem. Soc. Trans.
26, 370–374.
15. Jackson, S.K. (1997) Therole of...
... conclude, in doing an FTA, the speaker needs to balance three wants:
- The want to communicate content ofthe FTA
- The want to be efficient (or urgent)
- The want to maintain H’s face to any degree
1.3.2.3. ... deliberations of
the phenomenon. Addressing hedging, it can be defined plainly as theprocess whereby the
author reduces the strength of what he is writing”. Markkanen and Schroder (1985) define
hedging ... strategy of “saying less than one means”, the function of strategy being to modify
the writer’s responsibility for the truthfulness ofan utterance, to modify the attitude ofthe
author to the propositions...
... targeting, we divided it into three tri-glycine segments and
examined the importance of each domain in targeting specificity in vitro.
Replacement ofthe most C-terminal segment with alanine residues ... resulted
in mistargeting the protein to the stroma, while exchange of either of the
other two tri-glycine regions had no effect on correct targeting. Further-
more, simultaneous replacement ofthe ... N-terminal and middle tri-glycine
segments with alanine repeats did not cause mistargeting ofthe protein as
much as those ofthe N- and C-terminal, or the middle and C-terminal seg-
ments. These...
... events at the NBDs. If
TM12 is involved inthe coupling process between the
Fig. 2. Analysis of probe labelling of mutant TM12 isoforms of
ABCB1. For each ofthe mutant isoforms, densitometric analysis
was ... func-
tion is the molecular detail oftheprocessof coupling
between the NBDs and TMDs. The most striking evi-
dence for the presence of coupling between the two
domains is the ability of transported ... labelling is expressed
as the fraction of specific labelling of single-cysteine isoforms over the specific labelling ofthe G324C positive control. Values represent the
means ± standard errors of the...