the binding problem and the coherence of perception
Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
Ngày tải lên :
21/02/2014, 15:20
... 36% of the interaction of the full peptide with the binding cavity of SM3 The sizes of the surfaces were determined using distances between ligand ˚ atoms and protein atoms of less than A These ... a distance of A or less to the ligand The C-terminal part of the peptide (RPAP) has close contacts to the next protein in the crystal cell and is therefore stabilized on the surface of SM3 It ... Most of these contacts are with the binding cavity in the Fv domain However, there are significant additional contacts to the bottom of the next Fab (cf Figure 10) The surface covered by the nonbinding...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
Ngày tải lên :
07/03/2014, 17:20
... impaired in binding of the R13 region Both of these patterns contributed to the loss of binding contacts of mutant 72 Construction of a DA HbpR derivative In order to study whether the A-domain ... described in the main text Relevant restriction sites and the direction of transcription for the hbpR and the luxAB genes are indicated (A) (B) Detail of the region of the calmodulin -binding protein ... identify critical motifs in the binding site for HbpR and to examine the necessity of the sensing A-domain of HbpR for DNA binding The role of individual nucleotides and nucleotide motifs was characterized...
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Báo cáo khoa học: Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes ppt
Ngày tải lên :
30/03/2014, 02:20
... which the RNA and DNA backbones of RNA ⁄ DNA hybrids bind A phosphate -binding pocket and DNA -binding channel located in the DNA -binding groove are responsible for the specificity of RNase H for the ... activity, because the number of the metal -binding sites found in the crystal structures of the enzyme–metal ion complex formed in the absence of the substrate varies for different enzymes and different ... contrast, the RNA -binding groove is well conserved in the StoRNase HI structure The weak conservation of the DNA -binding groove may account for the ability of Sto-RNase HI to cleave double-stranded...
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the mit ress vision and mind selected readings in the philosophy of perception sep 2002
Ngày tải lên :
11/06/2014, 13:53
... account of the nature of the mind and the world?are problems at the heart of metaphysics It is often justiably said that the theory of perception (and especially vision) is the area of psychology and ... that the unaccustomed position of the ngers makes the synthesis of their perceptions impossible: the right side of the middle nger and the left side of the index not ordinarily work together, and ... existing between the parts of my body itself External perception and the perception of ones own body vary in conjunction because they are the two facets of one and the same act The attempt has...
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Grounding cognition the role of perception and action in memory, language, and thinking DIANE p
Ngày tải lên :
31/07/2016, 12:19
... and the force of the effectors The sensitivity to the current position of the limbs explains why in the crossed hand condition the hand closer to the handle is more activated As the frying pan ... are on the left, and the located objects (functional and nonfunctional) are on the right On each of the reference objects, the solid line runs through the center -of- mass of the object; the dashed ... simulation of their interaction with the objects, being sensitive to the relationship among the (irrelevant) object property, the handle location, the current position of their limbs in space, and the...
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huxley, aldous - the doors of perception
Ngày tải lên :
18/04/2014, 15:23
... qualitatively as they are in quantity Very often they set the tone of the whole work of art, they state the key in which the theme is being rendered, they express the mood, the temperament, the attitude ... hear the fantastic stories about the effects of Peyote and the nature of the ritual, which are told by the white and Catholic Indian officials in the Menomini Reservation None of them have had the ... tried to make the best of both worlds, indeed of all the worlds -the best of Indianism, the best of Christianity, and the best of those Other Worlds of transcendental experience, where the soul knows...
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Báo cáo hóa học: " Strong convergence theorem for a generalized equilibrium problem and system of variational inequalities problem and infinite family of strict pseudo-contractions" potx
Ngày tải lên :
21/06/2014, 01:20
... common element of the set of a generalized equilibrium problem of the set of solution to a system of variational inequalities, and of the set of fixed points of an infinite family of strict pseudo-contractions ... D(T) The class of -strict pseudo-contractions fall into the one between classes of nonexpansive mappings and pseudo-contractions, and the class of strong pseudo-contractions is independent of the ... Then, from Theorem 3.1, we obtain the desired conclusion □ Next theorem is derived from Theorem 3.1, and we modify the result of [11] as follows: Theorem 4.2 Let C be a closed convex subset of...
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Báo cáo y học: "Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on κ α NF-κB binding activity and TNF-α secretion of T cell" pot
Ngày tải lên :
09/08/2014, 03:24
... While the binding sites of W3/25 and OX35 are situated in the CC region of domain D1 and the BC region of domain D2 of the CD4 molecule, respectively [23], we could roughly localize the epitope of ... correlation between the degree of the ConA-induced proliferation of CD4+ spleen T cells and the severity of the arthritis score, i.e the lower the arthritis score, the higher the proliferation ... on the basis of a mean body weight of 175 g) on days 1, 0, 3, and 6, i.e day before AA induction, on the day of induction (day 0), and thereafter The lower dose of OX35 (2 mg) was chosen on the...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
Ngày tải lên :
18/02/2014, 08:20
... containing 100 lm of (GlcNAc)14 was analysed at the start, in the middle and at the end of each series of samples The resulting average values of the standards (displaying standard deviations of < 5%) ... LlChi18 after of incubation with nM of enzyme The double peaks represent the a- and b-anomers of the oligomers Using standard curves, the total concentrations of dimer, trimer and tetramer were ... into the chitinolytic potential of L lactis, the present results provide only the third example of the role of family 33 CBPs in the degradation of recalcitrant polysaccharides Furthermore, the...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
Ngày tải lên :
18/02/2014, 16:20
... expression level of the GFPtagged fusion proteins is indicative of that of the equivalent untagged proteins We examined whether the various charged-to-alanine substitutions affected the ability of full-length ... proteins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equivalent untagged proteins To assess the function of these proteins in endocytosis ... amounts of purified yeast actin were used in each binding assay and an amount representing 10% of the load used in each binding assay is shown (right) The lower panel shows GST only and the GST...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... as the ligand-macromolecule reaction occured A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio From the ... of stefin B and of stefin A (A) Far UV CD spectra of stefin A, stefin B and the P79S mutant (B) Near UV CD spectra of stefin A, stefin B and the P79S mutant that the protein aggregates heavily in the ... [12,14] Therefore, the differences in the far UV CD must be due to the contribution of tyrosines to this region of the spectrum [28] Figure 4B shows the near UV CD spectra of stefin A, stefin B, and the...
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Tài liệu Biodiversity and Local Perceptions on the Edge of a Conservation Area, Khe Tran Village, Vietnam doc
Ngày tải lên :
21/02/2014, 04:20
... zones, and on the different uses and valuation of species and of natural resources by the local people; (c) provide an overview of the importance of landscape and local species to the people of Khe ... according to the local point of view, the options for local people in the frame of the future nature reserve, the role they would like to play and the threats to biodiversity they identified; and (e) ... Phong My and Hoà Bac One characteristic of the village is the isolation of the houses from each other, and it takes approximately 30 minutes to walk from one end to the other of this village of 20...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
Ngày tải lên :
22/02/2014, 04:20
... albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained These data can be very useful in further studies of the toxicologically ... complexes of GGH, where the a carbon of the His residue is the sole source of chirality [10] However, while positions of the component dd bands and of CT transitions are relatively constant, their ... values) of Ni(II) and Cu(II) complexes of peptides at I = 0.1 M (KNO3) and 25 C Standard deviations on the last digits are given in parentheses EPR The X-band EPR spectra of Cu(II) complexes of VIHN...
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
Ngày tải lên :
06/03/2014, 01:20
... [20]: koff ẳ koff2ị ẵibuprofen=K2 ẵibuprofenị ỵ koff3ị ẵibuprofen=K3 ẵibuprofenịị ỵ kỵ off 7ị where koff(2) and koff(3) indicate values of koff occurring at K2 < [ibuprofen] < K3, and at ... affect the functional and structural properties of domains I and II [i.e of the 1382 region of (t)HSA] l The values of the Hill coefcients (n1, n2, and n3) for ibuprofen binding to tHSAheme and ... s)1) Ibuprofen binding to tHSAheme-Fe(II)-NO Figure shows the binding isotherm for ibuprofen binding to tHSAheme-Fe(II)-NO Analysis of the dependence of the molar fraction of the ibuprofenbound...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
Ngày tải lên :
06/03/2014, 11:20
... loop of antibody fragments (A) Replacement of the candidate location in NCS for grafting with the CDR loop of VHH (B) Insertion of the CDR 1-containing loop of the heavy chain in Fv into the candidate ... the design of and screening for small binding proteins The preparation of the small binding proteins with binding function further allows us to increase the binding strength by multibinding approaches, ... selected from a peptide library, and the replacement of material -binding peptide with the CDR loop of VHH and the local library approach in the CDR loop generated the VHH fragments with high affinity...
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Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc
Ngày tải lên :
07/03/2014, 00:20
... Synthesis of O1 DNA Synthesis of O2 and O1O2 DNAs Synthesis of O2 DNA Synthesis of O3 DNA Synthesis of O3 DNA Synthesis of S aureus cspC DNA Synthesis of S aureus cspC DNA (Fig 2A) was synthesized ... I in the presence of CI More precisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig 3B) The centers ... contents in the shifted complexes and and in the unbound labeled O1O2 DNA were determined by scanning the intensities of all the bands shown in the autoradiogram of the gel shift assay (I) and plotted...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... interactions between the N- and C-termini of the AR, and interferes with the binding of the coactivator TIF2 2676 Indirect Coactivator Increases interaction frequency with the AR Direct N- and C-Terminal ... Corepressor [86,91] There is evidence that interaction between the FXXLF (X = any amino acid) motif of the TAD and the LBD reduces coactivator recruitment and binding of the LXXLL motif of TIF2 [92] ... AR LBD via FXXFF and FXXMF motifs and enhances its activity in the presence of androgen The interaction between the N- and C-termini of the AR does not affect gelsolin FXXFF binding to AR LBD,...
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx
Ngày tải lên :
07/03/2014, 02:20
... determinants of the presence of Zn(II), and therefore of the Zn- or Cu-thionein character of the polypeptides Hence, the two full-length peptides, CeMT1 and CeMT2, give rise to mixtures of complexes, ... level to be of the highest interest, in order to shed light on the possible physiological functions of MTs in this organism and to further the understanding of the forces driving MT isoform differentiation, ... stoichiometry and the homogeneity of their preparations These differences are a result of the different coordination capacities of the respective C-terminal moieties and are attributable to the four...
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Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding affinity pptx
Ngày tải lên :
07/03/2014, 12:20
... of VH and VL interaction against relative affinity of the Fv to the antigen The plot is classified by the type of H39, as indicated Fig Scattered plot of OS-fitness against relative affinity of the ... absorbance, others showed a decreased or diminished antigen-dependency To evaluate the OS-fitness of the clone, the ratio of the specific absorbance in the presence of 10 lgÆmL)1 HEL to that in the absence ... to clarify the effect of the interface mutations on the antigenbinding affinity To investigate systematically this unsought relationship between the antigen -binding affinity of Fv and the VH ⁄ VL...
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The impact of aesthetic evaluation and physical ability on dance perception pdf
Ngày tải lên :
07/03/2014, 17:20
... about the video content, addressing various features of the stimulus movement (e.g., did the dancer jump?; did the dancer turn?; did the dancer’s hands touch the ground?) Also interspersed randomly ... interspersed randomly across the 128 experimental trials were 16 repetitions (8 trials with each of the dancers) of the 3-s videos of the dancers standing still in a neutral position The intertrial intervals ... that the interaction between liking and physical ability is represented within occipitotemporal and parietal regions of the AON We consider now how these findings inform our understanding of the...
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