... 5.2.4 In-situ Spectroscopic Measurements 117 vii 5.2.5 Data Analysis: TheApplicationofBTEMtoIRandRamanSpectroscopicData 120 5.3 Results: The Experimental Far -IR and Far -Raman Spectra ... determination ofthe number of observable species present and their pure component spectra, (2) determination of all the moles of all species, the number of reactions, andthe reaction stoichiometries, ... goals of this thesis are: (1a) the development of new and broadly-applicable algorithm, namely Chiral -BTEM (1b) theapplicationof this new algorithm to types of polarized spectroscopies and to...
... places the top n records at the top ofthe view Next, get the Freight value for the nth record and set the DataView filter to contain only rows with a Freight value greater than or equal to that ... as is the case with Freight In this case, iterate over the records following the nth record and add criteria to a copy ofthedata view filter to exclude them from the view Use either the primary ... combination of columns to identify the row to be excluded in each case Apply the new filter tothe view If the view is ordered on the primary key or unique columns in addition tothe top n columns,...
... applicationof this method by referring tothe actor andthe historian as the historian ofthe future” andthe historian ofthe past,” respectively.20 Regardless ofthe purpose for which it is used, therefore, ... interests The ideology is the smoke screen for their loot, the fictitious clothes spun by the intellectuals to hide the naked plunder ofthe Emperor The task ofthe historian, then, is to penetrate to ... with the choice of ends andofthe means resorted to for the attainment of these ends, and with the valuation ofthe outcome of actions performed.21 Furthermore, whether directed toward planning...
... = 150 N Problem 2-17 Determine the magnitude and direction ofthe resultant force F R Express the result in terms ofthe magnitudes ofthe components F and F andthe angle φ Solution: F R = F ... Problem 2-20 The plate is subjected tothe forces acting on members A and B as shown Determine the magnitude ofthe resultant of these forces and its direction measured clockwise from the positive ... Problem 2-41 Determine the magnitude and direction ofthe resultant FR = F + F2 + F ofthe three forces by summing the rectangular or x, y components ofthe forces to obtain the resultant force...
... requires a cytosolic chaperone-mediated association of precursor protein with peripheral translocase of outer membrane (TOM) receptors (TOM20, TOM22 and TOM70), which enables the translocation of ... targeted to mitochondria in transiently transfected hepatoma cells [22] The nature and specificity ofthe endoprotease andthe site of proteolytic cleavage remain unknown The same investigators were ... experiment, we determined the concentration of digitonin required to release mitochondria-associated CYP2E1 into the soluble fraction, and compare it with the amounts needed to release the outer membrane-specific...
... as the ligand-macromolecule reaction occured A binding isotherm was fitted tothe data, expressed in terms ofthe heat change per mole of ligand against the ligand to macromolecule ratio From the ... heavily in the presence of Cu2+ at pH andto a lower extent at pH Therefore, no comparison ofthe stability ofthe tertiary structure tothe effect of Cu2+ could be made Influence of copper on ... [12,14] Therefore, the differences in the far UV CD must be due tothe contribution of tyrosines to this region ofthe spectrum [28] Figure 4B shows the near UV CD spectra of stefin A, stefin B, and the...
... study and RT-PCR analysis of transcripts (A) Schematic representation ofthe pAO1 gene and ORF cluster flanked by Tn554 and DTn The cluster consists ofthe pmfR gene, encoding the regulator ofthe ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities ofthe proteins Our results demonstrate the presence of two pathways of ... members ofthe polyamine oxidases, the FAD cofactor was noncovalently bound tothe apoprotein andthe C-terminal fingerprint sequence SGGCY of monoamine oxidases, with C being the cysteine residue to...
... indirect effect on the binding environment of fosfomycin The direct influence comes from two hydrogen bonds formed by the nitrogen ofthe N-acetyl group andthe 3¢-oxygen tothe phosphonate and ... ligand binding The loop region ofthe upper domain moves towards the active site ofthe enzyme, thus shielding the substrates (and ligands) from bulk solvent A detailed thermodynamic study of ... independent ofthe thermodynamic and kinetic properties ofthe binding process and is solely based on the detectability ofthe peptides of interest We have used this method to show that binding of fosfomycin...
... important for the GABA transport activity 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role ofthe terminal structures ofthe N-glycans of GAT1, ... (Fig 2B,C), the large band is assigned to proteins with N-glycans of mature complex type andthe small band tothe proteins with N-glycans of mannose-rich type All bands ofthe mutants had reduced ... GAT1 tothe plasma membrane In order to study the influence of N-glycosylation on plasma membrane trafficking of GAT1, the distribution of wild type and mutants on the cell surface and in the cell...
... acid [40,41] They are prime target of ROS due to their location near tothe site of ROS production [42] and could be the source of carbonyl adducts Other recent reports have shown that there is intracellular ... between the relative rates of glycoxidation/oxidation and proteolysis in the mitochondrial matrix Although the respiratory function of mitochondria is not compromised in spite ofthe extent of altered ... ng atoms of oxygen consumed per minute and per mg protein The rate of oxygen consumption in state and in state 4, respiratory control ratio (RCR) of oxygen consumptions in states andand the...
... initiated through productive binding tothe substrate via two elements in ClpA, one in the N-domain andthe other in the pore ofthe ClpA hexamer In the case of short unstructured peptides or unfolded ... interaction between ClpS andthe N-domain of RR ⁄ AA, and this result suggests that neither the local nor the overall structure ofthe RR ⁄ AA mutant was compromised Mutation ofthe conserved arginine ... wished to compare the ability ofthe RR ⁄ AA mutant to bind tothe various substrates tested To this, we constructed a ClpA variant in which the glutamic acid residue within the Walker B motif of...
... mol) The results are mean ± SD, n ¼ 1–4 The degree of permeabilization is expressed as the percentage ofthe maximal value obtained at the end ofthe assay by the addition of mM Triton X-100 The ... Anderluh et al Stefin B and cellular toxicity A Insertion in monolayers B The ability of stefins and their aggregates to insert at the air–water interface, i.e in the absence of lipids, was determined ... pH 3.3 (up to 40% loss of viable cells) Therefore, the MTS test appears suitable for discriminating the cytotoxic effect ofthe stefin prefibrillar forms In order to determine whether the prefibrillar...
... reported for other members ofthe neurohypophysial hormone receptor family; for example, the distal N-terminus is required for agonist binding tothe OTR [11,12] and also tothe vasotocin receptor [13], ... NEN) [18] as the tracer ligand Binding data were analyzed by nonlinear regression to fit theoretical Langmuir binding isotherms tothe experimental data using the FIG P program (Biosoft, Cambridge, ... 1) Indeed, there is no correlation between the Ki and EC50 values for the mutant receptors (Fig 4) The ratio of EC50 to Ki is an indicator of efficacy, i.e the likelihood that a receptor will become...
... weight to asymmetry in these proteins The crystal dimer ofthe NBD ofthe maltoseuptake system of Thermococcus littoralis (MalK) consists of two monomers with deviation from perfect twofold symmetry ... from the NBD than ATP Rapid dissociation of hydrolysis product may be expected as part ofthe kinetics ofthe transport cycle ofthe intact transporter Furthermore, thedata demon- strate that the ... properties ofthe NBDs, rather than of their interactions with the TMDs (e.g [50] and see introduction for further references) Recent structural determinations of both intact ABC transporters and NBD...
... evaluation ofthe function ofthe three serine mutants, the kinetic properties ofthe mutant enzymes were compared to those ofthe parent enzyme, P-DN42 As shown in Table 1, the Km values of all ... ends ofthe putative b-strands, lining the face ofthe predicted active site cleft [15] These proposed structural features ofthe GT1 motif in GalNAc-T1 are similar tothe UBD, raising the possibility ... donor and/ or acceptor specificities Together with information on the three-dimensional structure ofthe GalNAc-transferases, this will allow an understanding ofthe catalytic mechanism(s) of these...
... appear to correspond tothe lower two immunobands of wild-type Kv3.1 In both cases, the upper band was darker than the lower band Additionally, the upper band was not visible in the presence of TM ... development 3288 Fig Topological model of Kv3.1 and amino acid sequences ofthe S1–S2 linker of Kvs (A) Topology of a Kv3.1 monomeric unit Black circles represent the Asn of native N-glycosylation ... utilized in rat brain and infected Sf9 cells, andthe role that occupancy of these sites has in the expression of functional Kv3.1s at the cell surface of Sf9 cells Immunoband patterns of wildtype Kv3.1,...
... in lanes and 11 Ó FEBS 2004 Analysis ofthe N-myristoylation consensus motif (Eur J Biochem 271) 867 The levels of incorporation of [3H]Leu and [3H]myristic acid into the lower band ofthe expressed ... incorporated into the upper ofthe two bands detected with [3H]Leu (lane 6) As there is no Met residue in the mature domain of TNF, this result indicates that the upper band corresponds tothe protein ... affects the susceptibility ofthe protein to protein N-myristoylation In this case, the presence of Lys at position was found to affect the amino acid requirement at position 3, and allowed Lys to...
... specificity of Ets factors is determined by their site of expression, their selective target sequence recognition, their selective abilities to interact with other transcription factor coactivators, and ... activates the PS1 ERM transactvates PS1 and does not require the )10 Ets element We have begun to identify the Ets factors controlling the expression of PS1 using the yeast one-hybrid selection andthe ... using the concentration of protein in the extract as an internal standard The activity of ()119/+178) PS1CAT in the presence ofthe empty vector was taken as 100% The values represented in the...
... ofthe effect of single point mutations on the solution structure of monomeric derivatives, we analysed the 2D NMR spectra ofthe different variants of mBS Figure shows the expanded regions of ... present A further indication ofthe similarity of their global fold to that ofthe parent protein is provided by the 1D H-NMR spectra (data not shown), which display all the characteristic signals ... 4.1 to RNase A, which has a thermal stability higher than that of mBS However, P19A/L28Q-mBS has the same thermal stability ofthe parent protein, in spite of greater similarity to RNase A To directly...
... capacity and also modulating the catalytic properties ofthe enzyme [19] To extend the information about the role ofthe noncatalytic SBD regions and their effect on the C-terminal CD, we further ... potential target for the manipulation ofthe level and quality of plant starch However, little is known about the role of SSIII in starch synthesis andthe structure–function relationship of this protein ... contains the catalytic site Thus, we evaluated possible intramolecular interactions between the N-terminal SBDs andthe C-terminal CD, and their effect on the regulatory properties of SSIII To investigate...