the active role of dendrites in the regulation of magnocellular neurosecretory cell behavior
phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation
Ngày tải lên :
15/11/2015, 12:54
... PS in the presence of physiological concentrations of calcium According to the manual instruction, the Kd for the binding of annexin V to PS has been estimated at 5.10-10 M The annexin V binding ... leading to a rapid increase of intracellular Ca2+ The increase of intracellular Ca2+ activates Gardos channel and scramblase The activation of the Gardos channel leads to an efflux of intracellular ... consequences The appearance of PS on the surface of the cell membrane can have major physiological consequences, including increased cell- cell interactions The increased adherence of PS exposing RBCs...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
Ngày tải lên :
16/02/2014, 09:20
... confirmed by Lin-54 binding to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86] ChIP experiments on the Cdc2 gene also provided insights into the association of other cell cycle ... proteins with the promoter E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85] Another E2F site distal to the E2F ⁄ CDE acts as an activating element binding ... However, as in most other CDE ⁄ CHR promoters, mutation of the CHR results in a smaller remaining cell cycle -regulation than alteration of the CDE [37] Interestingly, the core of the human Tome-1...
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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
Ngày tải lên :
18/02/2014, 08:20
... play a role in the regulation of adipose functions associated with obesity Results miR-27 inhibits adipogenic differentiation In order to investigate the role of miRNAs in the regulation of adipogenic ... initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum Cells were fixed on day of differentiation and stained with hematoxylin and eosin One of ... or without hematoxylin staining In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati, OH, USA) constantly maintained at 1% O2 Culture...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
Ngày tải lên :
19/02/2014, 16:20
... iron-peroxo intermediate [11] The sum of these ndings points at the involvement of more than one active oxidant in the diverse types of P450-catalyzed substrate processing [1215] The goal of the present ... alterations in the environment can cause drastic changes in the reactivity of the active species [76] Further support in favour of the idea of the involvement of a high-valent iron-oxene in P450 ... reect ring opening of an epoxide intermediate at either of its two C-O bonds [219,220] The sum of these ndings strongly invokes the notion of oxygen activation during redox cycling of the diverse...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
Ngày tải lên :
07/03/2014, 14:20
... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme To obtain an insight into ... 1), thereby confirming the crucial role of Asp142 Catalysis in family 18 chitinases (Eur J Biochem 271) 259 In most ChiB structures, Asp142 is clearly defined, being either in the ÔdownÕ or in the ... protons at the pH where the enzyme is most active The basic arm of the pH-activity profile must be determined by the loss of one or both of these protons Comparison of rows 1, and with rows and in Table...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
Ngày tải lên :
08/03/2014, 08:20
... of GSK3 (using Li or SB-415286) appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant ... p38 MAP kinase in a human intestinal epithelial cell line, HT-29, and promote the transcription of the interleukin-8 gene [42] In line with previous studies, Fig 7A shows that Li induced the phosphorylation/activation ... (1994) The inhibition of glycogen synthase kinase-3 by insulin or IGF-1 in the rat skeletal muscle cell line L6 by wortmannin, but not rapamycin: evidence that wortmannin blocks activation of the...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
Ngày tải lên :
15/03/2014, 11:20
... number of amino acid residues contribute to the binding of the substrate by hydrogen bonding and ⁄ or hydrophobic interactions Using the ligplot program [31], we investigated the specific interactions ... important role in ‘cycling’ the pKa of catalytic Glu during catalysis [24,38] The mutation of the D1 residue to Asn (D140N) in SmChiB caused a 500-fold decrease in activity [39] On the other hand, in ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars and the side-chains of three acidic residues in the conserved...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
Ngày tải lên :
15/03/2014, 23:20
... polymers The nature of the aromatic residues in the two triads might then be reflecting the different functional constraints for the two proteins: Exg requiring precise positioning of laminarin substrate ... seen for the Phe triad in the structure of the mutant Exg:L3 complex Neither of the two sugar residues interacting with the Trp triad overlaps with the sugar seen in the Phe-Phe clamp of Exg Structural ... Finally, the unexpected discovery of an isolated binding site remote from the active site poses the intriguing possibility of a novel evolutionary solution to the problem of maintaining association...
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Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx
Ngày tải lên :
16/03/2014, 06:20
... structure of rat MVK bound to ATP [17], depicted in Fig 9A, the active site of the enzyme is located at the interface of the N- and C-terminal domains of the monomer The adenosine base of ATP is ... the site of ATP binding and may exploit common side chain interactions; or (b) the conformations of residues involved in RNA binding may be directly or indirectly coupled to the binding of ATP ... to the identification of a novel RNA binding protein that mediates the post-transcriptional regulation of LHR mRNA in the ovary [10–12] This protein, initially named LHR mRNA binding protein (LRBP),...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
Ngày tải lên :
16/03/2014, 18:20
... ERM being the most related [35] PEA3 members all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory ... marks the position of supershifted complexes (B) The binding of in vitro translated ERM is eliminated by mutations in Ets motifs The binding of in vitro translated ERM over the PS1 promoter was ... complexes with in vitro translated ERM which could be supershifted by the antibody, indicating the presence of ERM binding site(s) in each of the three probes We also examined the pattern of complexes...
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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
Ngày tải lên :
17/03/2014, 09:20
... catalytic role of these domains Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner core of the complex, indicative of the biological significance of the ... confirmed the inhibition of the overall reaction by the low molecular mass disulfides [41] The data pointed to the exchange of redox equivalents between the complexes and the medium, involving the dihydrolipoate ... activity in the nonspecific insulin reduction test, mitochondrial thioredoxin is by far the most effective in protecting the complexes While some of thioredoxins are inactive or even decrease the complex...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... > h from the start of glucose infusion For the calculation of glycogen C6 changes, data acquired within h of the start of the glucose infusion was not included to avoid any in uence of transient ... the initial rate of label incorporation into glycogen C6 relative to the rate of label incorporation into glycogen C1 reflects the relative isotopic enrichment of Glc6P in the C6 relative to the ... should the tail vein fail, which was the case in only three animals, the data of which were included After the lines were inserted, pentobarbital was infused continuously at 4.8–6.0 mgÆkg)1Æh)1 The...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên :
17/03/2014, 17:20
... following the binding of anthranilate (Fig 3, inset) and the shoulder at % 500 nm following the binding of benzoate (De497nm of 7500ặM)1ặcm)1 vs a gure of 2000 4000ặM)1ặcm)1 observed with the other ... in binding were observed for Y238 mutants with the ligands tested (Table 2) These results indicate that the mode of ligand binding is retained in the two mutants, and that the alteration of the ... in pkDAAO and Y238 in RgDAAO share the characteristics of being exible and adapting their conformation depending on the size of the ligand side chain [27] Our results indicate that the role of...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... Tyr These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the ... that the binding of both the substrate NIPAB and the product PAA are significantly altered by mutating the phenylalanines in the active site suggesting that hydrophobic interactions between the ... aromatic phenylalanines and the phenyl ring of the substrate play an important role in substrate binding The large effect of the mutations on the acylation rate indicate that these residues are...
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Explaining Bank Failures in the United States: The Role of Self-Fulfilling Prophecies, Systemic Risk, Banking Regulation, and Contagion pdf
Ngày tải lên :
22/03/2014, 17:20
... underscoring the importance of introducing fixed effects into the present count regressions Then again, in the case that determinants interrelate with the number of bank failures—meaning that the strict ... out insolvent depository institutions the sharp increase in bankruptcies of banks at the end of the 1980s was followed by an equally dramatic decrease at the beginning of the 1990s Table of the ... restructuring of the industry or they mark the occurrence of a more profound instability in financial intermediation Furthermore, variables designating e.g the duration of a crisis are uninformative...
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AN INCREASING ROLE FOR COMPETITION IN THE REGULATION OF BANKS doc
Ngày tải lên :
29/03/2014, 08:20
... as the development of offshore financial centers and off-balance-sheet methods of financing); • the diminishing effectiveness of traditional controls due to financial innovation (including the ... 1980s In the case of S&Ls the insurance premium was set by statute in 1950 at 1/12 of 1% of the assessable deposits and was the same for all insured institutions regardless of the riskiness of their ... to being changed and enforced in the interest of the regulated industry, rather than in the interest of the economy at large General laws, on the other hand, tend to be more immune and therefore...
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báo cáo sinh học:" The role of regulation in influencing income-generating activities among public sector doctors in Peru" doc
Ngày tải lên :
18/06/2014, 17:20
... reports of doctors holding more than two different jobs to supplement their income "In the past 10 years the classical form of dual practice of a public job in the morning, doctor's office in the ... study has highlighted the role of regulation in reconciling the collective interests within the medical profession with broader policy objectives such as maintaining staff in the public sector and ... three were office bearers within the Peruvian College of Physicians The in- depth interviews were tape-recorded and transcribed in Spanish The findings were grouped into themes to address the general...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
Ngày tải lên :
20/06/2014, 04:20
... heal the defect The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs ... Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells were incubated for 15 minutes at 37°C in ml PBS containing tritiated ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing MC3T3-E1...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
Ngày tải lên :
20/06/2014, 07:20
... heal the defect The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs ... Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells were incubated for 15 minutes at 37°C in ml PBS containing tritiated ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing MC3T3-E1...
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Báo cáo y học: " Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b" doc
Ngày tải lên :
09/08/2014, 07:20
... activity We further investigated a putative role for MAPKs in the TNFα-induced increase in Cyp7b activity by using the MEK1 inhibitor PD98059 and the p38 inhibitor SB203580 The p38 inhibitor (SB203580) ... investigate whether the effect of SN50 interfered at the level of Cyp7b activity or expression, we also analyzed the influence of SN50 on the TNF-α-induced increase in Cyp7b Available online http://arthritis-research.com/content/7/6/R1271 ... absence (-) of the mitogen-activated protein kinase (MAPK) kinase (MEK)1 inhibitor PD98059 (PD) or the p38 inhibitor SB203580 (SB) Thereafter, cells were incubated in the presence or absence of 0.5...
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