synthesis purification and formulation of 11c toluene

Purification and cloning of PCR products

Ngày tải lên : 25/10/2013, 22:20

... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to ... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors...

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Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf

Ngày tải lên : 18/03/2014, 01:20

... was tested by formation of the complex between RasGTPcNH2 and the Ras-binding domain of the Ras effector protein c-Raf-1 MATERIALS AND METHODS General description of synthesis High pressure liquid ... methanol, again evaporated and dried over P4O10 Monoamido-phosphoric acid, H2PO3NH2, was prepared as described [11] Synthesis of GTPcNH2 and GTPcF Fig Chemical structure of the nucleoside triphosphate ... methanol and repeated evaporation under reduced pressure, yield of the pure GTPcNH2 was 50–60% One gram of GTP triethylammonium salt was added to a stirred solution of 2.5 mL tributylamine and 1.2...

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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt

Ngày tải lên : 23/03/2014, 15:20

... AJ888885) Comparison of genomic and cDNA revealed two introns with a size of 58 and 64 bp Removal of the introns led to an open reading frame of 465 amino acids and a molecular mass of 51.41 kDa, which ... observed Formation of sterigmatocystin (ST), a precursor of aﬂatoxin B1, the synthesis of ascoquinoneA, a polyketide giving the sexual ascospores the red-brown colour and synthesis of naphtopyrone, ... different degree of disruption of the mycelium and some loss of the acyl-CoA during the puriﬁcation procedure Anyhow, an approval of the procedure with known concentrations of acetyl-CoA and propionylCoA...

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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot

Ngày tải lên : 20/06/2014, 23:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

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Báo cáo hóa học: " Hydrothermal Synthesis, Microstructure and Photoluminescence of Eu3+-Doped Mixed Rare Earth Nano-Orthophosphates" pot

Ngày tải lên : 21/06/2014, 08:20

... (TG) and differential scanning calorimetry (DSC) with a heating rate of 10°C min-1 Results and Discussion Experimental Section Crystal Phase and Microstructure of Mixed Rare Earth Phosphates Synthesis ... 0.9 mol With the 1:9 M ratio of Gd3? and Y3?, Y0.9Gd0.1PO4 present to the pure tetragonal phase In one word, because the ion radii of Y3?, Gd3? and La3? are 88, 93.8 and 106.1 pm, respectively, ... difference in radius between La3? and Gd3? (Gd3? and Y3?) is smaller than that of Y3? and La3?, so the product can present the pure phase with the different content ratio of the rare earth ions Besides,...

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SYNTHESIS, PROCESSING AND CHARACTERIZATION OF NANOCRYSTALLINE TITANIUM DIOXIDE

Ngày tải lên : 02/07/2014, 12:57

... nanostructures constitutes the basis of nanoscience Manipulated control of the properties and response of nanostructures can lead to new devices and technologies The synthesis of nanosized materials is ... calcinations time and temperature have a great influence on the particle size and phase purity of the final powder 2.6 Sintering of Nanopowder Synthesis, characterization and processing of nanocrystalline ... sintered at (a) and (b) 1300oC; (c), (d) and (g) 1400oC, (e) and (f) 1600oC for h at ambient atmosphere 49 Figure 20 Variation of Vickers hardness and compression strength of TiO2 ceramics...

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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc

Ngày tải lên : 07/08/2014, 20:23

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps

Ngày tải lên : 12/08/2014, 01:21

... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...

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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc

Ngày tải lên : 12/08/2014, 04:21

... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...

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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Ngày tải lên : 11/09/2015, 10:06

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...

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SYNTHESIS, CHARACTERIZATION AND APPLICATIONS OF

Ngày tải lên : 11/09/2015, 10:19

... grateful to Professor Hidajat Kus for his help and support I also extend my appreciation to Prof Chung Tai Shung, Prof Hong Liang, Prof Kang En Tang, Prof Tan Thiam Chye, and Prof Song Lianfa ... production in steam reforming of ethanol (SRE) and CO2 reforming of ethanol (CRE) A fundamental understanding of the cause of the high activity and the stability of Rh/oxide-nanotube catalysts ... recent synthesis and applications of oxide nanotubes and mesoporous materials attract intense research interests due to their chemical and physical properties This thesis reports the synthesis and...

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Synthesis, assembly, and applications of titanium dioxide nanostructures and related nanocomposite

Ngày tải lên : 13/09/2015, 21:39

... SYNTHESIS, ASSEMBLY, AND APPLICATIONS OF TITANIUM-DIOXIDE NANOSTRUCTURES AND RELATED NANOCOMPOSITES LI JING (BEng & MEng, Tianjin University, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... University, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2007 ...

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Transition metal oxide nanostructures shape controlled synthesis, characterizations and studies of physical properties

Ngày tải lên : 14/09/2015, 14:11

... edge and core region of a NW of diameter ~80 nm A low magnification TEM image of the same NW is displayed in the inset of (d) and (f) its SAED pattern (g) and (h) HRTEM images of Nb2O5 NWs of diameter ... captured near the edge and core region of a NW of diameter ~180 nm Inset of (a) is a low magnification TEM image xii LIST OF FIGURES of the same NW and (c) its SAED pattern (d) and (e) HRTEM image ... SEM image of the cobalt foil with nanowires after minutes of growth and (d) SEM image of the same region of the foil taken after a subsequent minutes of growth (e) a typical TEM image of the Co3O4...

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Synthesis, properties and cytotoxicity of nanostructured nickel ferrite particles

Ngày tải lên : 14/09/2015, 17:50

... (b) the composite of C1 and PLA and (c) the composite after immersed in DCM for 30 Figure 4-4 SEM images of (a) B1+S and (b) the composite of B1+S and PLA Figure 4-5 SEM images of (a) B2, (b) B2/PLA, ... Calculation of anisotropy constants (K) in C1 and C2 Table 3-5 Calculated crystallite sizes of B1 and B2 estimated using Scherer’s formula Table 3-6 Crystallite sizes and strains of B1 and B2 estimated ... the edge of one specific particle of C2 Figure 3-7 Relationship between zeta-potential and pH for C1 and C2 Figure 3-8 TGA results of C1 and C2 The arrows represent the onset points of the second...

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purification and characterizations of phytase enzyme form aspergillus niger

Ngày tải lên : 06/10/2015, 13:05

... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...

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Synthesis, characterization and application of nanostructured metal oxide

Ngày tải lên : 16/10/2015, 15:37

... bottom of the conduction band; EF the Fermi level; XS the work function and XSO the electron affinity Figure 4-2: Energy band diagram of metal and semiconductor contact Figure 4-3: Energy band diagram ... the number of pairs of electron hole form per quantum of photon, k is the optical absorption coefficient and I is the intensity of photon Figure 4-7: Schematics of the circuit diagram of photo-illumination ... characteristics of the effect of O2 on MoO3 nanobelt Figure 5-4: I-V characteristics of the effect of NH3 on MoO3 nanobelt Figure 5-5: Comparison of the effect of different chamber pressures of ammonia...

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EMPIRICAL STUDY ON EFFICIENCY AND SUSTAINABILITY OF SOIL PURIFICATION FACILITY FORDISCHARGED POLLUTANTS FROMROAD SURFACE

Ngày tải lên : 05/09/2013, 09:08

... decrease of penetration capacity and purification efficiency during continuous use of the facility was investigated The facility consisted of two tanks In one of the tanks, continuous use of the ... 45 Upper layer Lower layer of weir (cm) (cm) (cm) Akadama sand (5) Pit sand (40) Pit sand (45) Journal of Water and Environment Technology, Vol.3, No.2, 2005 water and Overflow water were measured ... content in pit sand The initial nitrogen content of Akadama sand in Tank A was higher than that of pit sand of Tank B The nitrogen content in both Tanks increased with time In both Tank A and B, the...

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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt

Ngày tải lên : 12/02/2014, 10:20

... growth and mortality of insect pests, Plutella xylostella (PX) and Spodoptera litura (SL).17th International Congress of Biochemistry and Molecular Biology Annual Meeting of the American Society of ... stop codon and Xho I restriction site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the synthetic ... known, Mr of MCoTI-II is about 3.4kD, hence Mr of the obtained recombinant fusion protein should be about 58 kD ( the sum of 55 + 3.4kD of MCoTI-II) As seen on Fig 10 the 58 kD protein band was...

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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt

Ngày tải lên : 19/02/2014, 07:20

... shown) Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and the ﬁrst-strand cDNA for 3¢ rapid ampliﬁcation of cDNA ends (RACE), prepared from total RNA of 6004 Nile ... including 56 bp of 5¢ UTR, 1482 bp of ORF, and 299 bp of 3¢ UTR The coding region of the sequence was translated into 494 amino acids, which included a typical signal peptide of 18 amino acids and two ... total volume of 50 lL containing 0.75 lL of a template, 150 pmol of a forward primer (A), 150 pmol of a reverse primer (B or C), · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5...

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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx

Ngày tải lên : 20/02/2014, 01:20

... 341 nt of the 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand ... basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being at that time unavailable In the present study we investigated the involvement of sequences ... sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA Results Effect of mutations in the 3¢- or the 5¢-end of the (–)IRES...

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