... excluding a putative N-terminal signal sequence Primers Orf10F (GGTGTCCGAGCCCACGACGGT) and Orf10R1 (catggctcgaGTCACAGCTCAGTGCG) were used to amplify a 431-base pair (bp) sequence of c19orf10 encompassing ... vector (Novagen, part of EMD Biosciences, Inc., San Diego, CA, USA), resulting in a glutathione S transferase (GST)-His-c19orf10 fusion gene Primers Orf10NdeI (gaattccatatGGTGTCCGAGCCCACGA) and Orf10R2 ... the maintenance of synovial inflammation, aggravating the destruction of cartilage and bone and stimulating the development of the pannus Because FLSs can exhibit significant phenotypic changes...
... characterizationand processing of nanocrystalline materials are part ofa fast emerging and rapid growing field in nanoscience and nanotechnology Nanocrystalline materials show interesting properties ... members and evaluating my thesis My thanks also extend to Department of Mechanical Materials and Aerospace Engineering (MMAE), Advanced Materials Processing and Analysis Center (AMPAC) and UCF for ... used as a purging gas at a speed of 10 cm3/min 3.3.2.2 X-ray diffraction Phase characterizationand calculation of average grain size of the synthesized powder calcined at 400oC, 600oC and 800oC...
... 2006 Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 Jain S K, Jain A, Hannurkar P R, Kotaiah S 2007 Characterizationof plasma parameter, ... mirror magnetic field, and (b) flat magnetic field the magnetic field can cause diffusion of the plasma particles to the wall of the plasma chamber The measured magnetic field profiles along the axial distance ... using Poisson software for (a) mirror magnetic field, and (b) flat magnetic field are shown in figure The calculation was also performed analytically (Montgomery 1966) using standard relations for calculating...
... AAA AGC AGG CTA TCA TGC TGT TGT CAG GTC CCT CTC G; and reverse, GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC AGT GGT GGT GGT GGT GGT GCA GAG GAG GGA TAT GGG GAA CGG CAA A The PCR reaction was done ... GTT GGA ATT CCA TCA TCA TCA TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAG GAG GGA TAT GGG GAA C The PCR reaction was done as described above The amplified ... Experimental procedures Isolation of the tyrosinase gene from Trichoderma reesei The tyr2 gene was amplified from genomic T reesei DNA with the following primers: forward, GGG GAC AAG TTT GTA CAA AAA AGC...
... Denmark 47 Kamiya, H & Ogata, K (1982) Hemagglutinins in the acorn barnacle Balanus (Megabalanus roseus): purification andcharacterization Nippon Suisan Gokkaishi 48, 1427 48 Kamiya, H., Muramoto, ... Sucrose, glucose and glucose-6-phosphate inhibit at concentrations less than 25 mM However, N-acetyl derivatives, namely N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc) and N-acetyl mannosamine ... by affinity chromatography It yielded a 2000-fold increase in specific activity Analysis of the lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa The binding affinity of...
... Dr Ake Engstrom at the Peptide Synthesisand Analysis Laboratory ¨ of the Department of Medical Biochemistry and Microbiology, Uppsala University The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and ... Wei and Matthews [4] Suc-Ala-His-Pro-Phe-pNA was purchased from Bachem AG, Switzerland Malachite green reagent was obtained from Apoteksbolaget, Sodersjukhuset, ¨ Stockholm, Sweden MDEA was bought ... homologous to peptides of the purified porcine protein The sense primer (5¢-ATG GCG GTG GCG GAC CTC GCT CTC AT-3¢) corresponded to bp 9–34 of AF164795 and the antisense primer (5¢-TCA GTA GCC GTC GTT...
... Anaerobic lactate ¨ oxidation to CO2 by Archaeoglobus fulgidus via the carbon monoxide dehydrogenase pathway: demonstration of the acetylCoA carbon-carbon cleavage reaction in cell extracts Arch ... by bp) The region 81–65 bp upstream of the start codon of AF499 was identified as an archaeal promoter element by sequence analysis The sequence AAAGGTTAATATA shows a high level of identity with ... sample; it probably forms aggregates that not run into the gel (lane B2) This behavior is typical of integral membrane proteins The polypeptide with an apparent molecular mass of 53 kDa appears...
... GmPDIL-1 and GmPDIL-2 by PCR using the oligonucleotide primers 5¢-GACGACGACAAGATGGAG GAATCATCGGAGAAAGAGTTC-3¢ and 5¢-GAGGAGA AGCCCGGTTCAAAGCTCATCTTTTCCTTTTTC-3¢ for GmPDIL-1, and 5¢-GACGACGACAAGATGCTCACCGA ... 5¢-CCCAATTTGGAAGCTGATCACAT-3¢ and 5¢-CTTCCTTGGTCCTACCCCCTTCGT-3¢ for GmPDIL-1, and 5¢-AGCCCGAGGTGGACGAGAAGG-3¢ and 5¢-T TTGCCACATCAGGATCCACAGTTT-3¢ for GmPDIL-2, and sequencing His-tagged expression ... (TaKaRa Bio Inc.) Forward primers 5¢-GACCTGTTATC CAACCGTGTACTTCAGGT[FAM]C-3¢ and 5¢-CGGAAC GCCAATTCATTCTCTTC[FAM ]G- 3¢, and reverse primers 5¢-GCAGGTTTGTCCCGGTTCT-3¢ and 5¢-GCTCAAGG GCGAAGACGTAA-3¢...
... inserting oligonucleotides containing three copies of DR2 (ACGCTCACTGGAACACT GGAATGCCCAGTTCTCGTCGCTCACTGGAACACTG GAATGCCCAGTTCTCGTTCGCTCACTGGAACACTG GAATGCCTCTAG) upstream of the thymidine-kinase ... Journal compilation ª 2006 FEBS 401 S mansoni NR1 W Wu et al DR1: 5¢-CCGTAAGGTCACAGGTCACTCG-3¢, DR2: 5¢-CCGTAAGGTCACAAGGTCACTCG-3¢, DR3: 5¢-CCG TAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAA GGTCACAGGAGGTCACTCG-3¢, ... 5¢-ATTTCAGAAGTTGAAC AAACACAC-3¢, reverse primer: 5¢-AAGATGGTATT GAAGATGATGGTTGA-3¢), purified from agarose gel using Gel Extraction kit (Qiagen, Valencia, CA, USA) and randomly labeled with 32P using a Metaprime...
... (5¢-GGCCTCATGAAGAAAAAGGTCGTCA TAATT-3¢), and TG101 (5¢-GGCCAAGCTTCTAGAAC TTGAGAACCCTAGC-3¢) (for the P horikoshii CoADR), and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTA GTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAA CTTCAAAACCCTGGC-3¢) ... the large pellet of heat denatured protein obtained at this stage) The enzyme shows a single band corresponding to a m ¼ 49 k on SDS ⁄ PAGE (Fig 5) and Production and purification of recombinant ... blast and tfasta analysis of the phCoADR revealed a significant level of identity to putative NADH oxidases from hyperthermophiles and bacterial NADH oxidases from mesophilic sources (Fig 1) Of...
... AGCC (G/ C)CTG-3¢ and 5¢-AACCATGGCCAGCCAC 274 A Goyer et al (Eur J Biochem 269) GCCGAGAAGCC (G/ C)CTG-3¢ were used PCR products were separated by electrophoresis on a 0.8% agarose gel A 600-bp fragment was ... reverse transcriptase (RT), and lM of the degenerated reverse primer 5¢-GCGGATCCTTA (G/ C)ACGGCGGCGAAGTAC TCC-3¢ After reverse transcription for 30 at 42 °C, the ®rst-strand cDNA was ampli®ed in a PCR ... BAS1 proteinof Brassica, spinach, barley andA thaliana, PR1 of Phaseolus and MHF ofA thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley,...
... from strain Iso1 was amplified by PCR using proof reading Pfu DNA polymerase The universal primers 5F (5¢-TGAAGAGTTT GATCATGGCT-3¢) and 1540R (5¢-AAGGAGGTGAT CCAACCGCA-3¢) numbered according to the ... ShineDalgarno sequence and the N-D-AAase start codon The sequence upstream of the N-D-AAase gene is: 5¢…AGGACAGACGAATG…3¢ (The Shine-Dalgarno sequence and start codon are in bold) The recombinant ... amidohydrolase; A- 6-D50061: Alcaligenes xylosoxydans ssp xylosoxydans A- 6 N-acylD-glutamate amidohydrolase; Alicaligenes faecalis-DA1: Alcaligenes faecalis DA1 N-acyl–D-amino acid amidohydrolase; V paradoxus...
... CGTGCTCGTAAACGATGCGTATTAC ACAATCTCTTTGCCGGCCTCCGC GGCGCGACGCACGAAAATTACGC GTCTATTTTCACGCAAAGCACCCGGT AAACCGATTTGTACATCGCATTTTC CATTAATGGATATCGTTCCGATTCC firmed by sequencing (Invitrogen Biotechnology Co., ... are underlined Name Sequence of primers (5¢- to 3¢) P1 P2 D232N-F D232N-R K270H-F K270H-R R403Y-F R403Y-R GGTACCATGAATGATGCAGCAAAAG (KpnI) GAATTCTCAGCCTGATATTTCCGCCT (EcoRI) CGTGCTCGTAAACGATGCGTATTAC ... mM L-aspartate was used as amino donor for a- ketoglutarate, and 30 mM L-glutamate was used as amino donor for oxaloacetate The activity of a- ketoglutarate was adjusted to 100 L-aspartate Fig Purification...
... of P furiosus using the primers BG1279 (5¢-GCGCG CCATGGCATCCGAGAAGATGGTTGCTATCA, sense) and BG1297 (5¢-GCGCGGGATCCTCATTTAAGCAT GAAAACAACTTTGCC, antisense), containing NcoI and BamHI sites (underlined ... The Authors Journal compilation ª 2006 FEBS R Machielsen and J van der Oost 14 Higashi N, Matsuura T, Nakagawa A & Ishikawa K (2005) Crystallization and preliminary X-ray analysis of hyperthermophilic ... Invitrogen (Paisley, UK) and New England Biolabs (Ipswich, MA, USA) Pfu Turbo and T4 DNA ligase were purchased from Invitrogen and Stratagene (Amsterdam, the Netherlands), respectively For heterologous...
... amplify a 1203-bp sequence encoding the full-length d-CDes protein: primer 102 (5¢-CGGATCCAGAGGACGAAGCTTGACA-3¢) extended by a BamHI restriction site and primer 103 (5¢CTGCAGGAACATTTTCCCAACACC-3¢) ... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Purification andcharacterizationof ... standard The DNA and amino acid sequence analyses and prediction of the molecular masses were performed with the programs mapdraw and protean in dnastar (Lasergene, DNASTAR, Madison, WI, USA)...
... (5¢-GTCGTCTTGCGGTCACCT GGACATGCATCAACGAACAG-3¢) and Ct-Aspf1 (5¢-GT CGTCTTGGATCCTCTCGAGTCTCAATGAGAACACA GTCTCAAGTC-3¢) These primers contained BstEII and BamHI sites and were used to generate a ... fumigatus allergen Aspf1 Martı´ nez-Ruiz A, Garcı´ a- Ortega L, Kao R, Lacadena J, Onaderra M, Mancheno JM, Davies J, Martı´ nez del ˜ ˜ Pozo A & Gavilanes JG (2001) RNase U2 and a- sarcin: a study ... mutant Aspf1D(7–22), using the mutagenic primer: 5¢-GTCACCTGGACATGCGGCGGCCTTCTATACAAT CAA-3¢ The integrity of both sequences was confirmed by DNA sequencing All of these procedures were also as...
... (5¢-TCTCGTGGAAGAAATCGACA-3¢) designed based on the sequence of fragment CaLP1 obtained above anda reverse adaptor primer R2 (5¢-TCGAATTCGGATCC GAGCTC-3¢), using the above first-strand cDNA got as template ... sequence of CaLP2 were used in the nested PCR reactions The first round of PCR reaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ ... (5¢-ATGGCGGAAGATC TCACAGAAGAACAAA-3¢) and G2 (5¢-TCATTTATTTT CTTGTTGCTGTTC-3¢) Preliminary experiments showed that a total RNA concentration of lg and 25 cycles were well within the linear of amplification...
... estimated by bootstrap analysis (100 replications) SDS/PAGE SDS/PAGE was performed with a stacking gel of 7.5% (w/v) acrylamide anda separating gel of 12.5% (w/v) acryl amide, as described by Laemmli ... EC (Fig 3A) These results indicated that the b-aryl ether cleavage enzyme accumulated and was stable in the extracellular fraction The extracellular fraction of 2BW-1 generated abundant GG and ... substrates GOUbz Ó FEBS 2003 2360 Y Otsuka et al (Eur J Biochem 270) Fig Mass spectra of guaiacylglycerol and guaiacol, products of the b-aryl ether cleavage reaction The reaction products of GOG generated...
... concentration using Kaleidagraph (SYNERGY Software) to fit the data to the Michaelis-Menten equation All reducing sugar assays included a glucose standard curve The average molecular mass of the XG ... activity on G4 , G5 and G6 and only faint product bands were produced after overnight digestion (Fig 2C) Xeg74 was active on both bean and tomato XG and inactive on boiled barley b-glucan (Fig ... et al (Eur J Biochem 270) Fig Example ofa xyloglucan (XG) oligosaccharide structure (XXLG) and nomenclature [5] XXXG, XXLG, XLXG, and XLLG are known subunits of tamarind seed xyloglucan (tamXG)...