studying idp stability and dynamics by fast relaxation imaging in living cells
nmr methods for the investigation of structure and transport
Ngày tải lên :
29/05/2014, 18:32
... gain by measuring in the time domain and computing the spectra by Fourier transformation of the measured data This subsequently evolved into multidimensional NMR and NMR imaging, where the demands ... graduate students and scientists working in the chemical and engineering sciences It is written with great insight into both the NMR methodology and the demands from the engineering community I ... engineering (flow and growth in a biofilm reactor), and food process engineering (temperature mapping during microwave drying, droplet-size distribution in emulsions) Exemplary implementations in...
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Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx
Ngày tải lên :
11/08/2014, 05:21
... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... acquisition, and interpretation of data, and has been involved in drafting and critically revising the manuscript for important intellectual content All authors have read and approved the final submitted ... planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the manuscript for important intellectual...
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báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot
Ngày tải lên :
11/08/2014, 16:21
... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... acquisition, and interpretation of data, and has been involved in drafting and critically revising the manuscript for important intellectual content All authors have read and approved the final submitted ... planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the manuscript for important intellectual...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
Ngày tải lên :
18/02/2014, 06:20
... addition is indicated by broken lines Side chain amide correlations of glutamine and asparagine residues are connected by a continuous line Chemical shift changes of backbone amide HN (B) and 15N ... chains of the Ser53 and Ser57 in the interhelical linker, forming a highly conserved phosphorylation motif, are visualized in ball -and- stick format In contrast to the well-defined helices and ... residues in the interhelical linker and the intermediate 15 N-1H NOEs observed for the C-terminal half of VpUcyt indicate a substantial amount of remaining conformational flexibility PRE and secondary...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
Ngày tải lên :
18/02/2014, 16:20
... characterize ATP-binding and GTP-binding proteins in cells, we profiled purine-binding proteins on a proteome scale [40] For this purpose, we used 5¢-p-fluorosulfonylbenzoyladenosine (FSBA) (Fig ... that binds proteins in their nucleotide-binding region and then covalently modifies nucleophilic amino acids (especially tyrosine and lysine) in its proximity [75] In the past, FSBA was mainly ... pattern of silver-stained proteins pointed to candidate ATP-binding or GTPbinding proteins that were selected from the 2D gel and identified by MS In this way, 12 different proteins could be identified...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
Ngày tải lên :
22/02/2014, 08:20
... coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it was shown that folding topology plays a role on the protein ... bending rigidity) and contour length, enabling the validation of the continuum model of biomolecules such as microtubule and DNA In last decade, since the pioneering works by Bustamante and coworkers13,14 ... silk is represented by hierarchical combination of nonlinear elastic springs, inspired by AFM experimental results by Hansma and coworkers.4 Kasas et al32 had established the continuum model (tube...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
Ngày tải lên :
31/03/2014, 07:20
... steps on IgG- and calmodulin-containing resins, respectively The immobilized protein is specifically eluted in the first instance by protease cleavage (TEV) and in the second step by lowering the calciumdependent ... datasets generated in this way can be mined for information that is not necessarily provided by individual experiments and thus increase the overall insight gained into the system under investigation ... PSD95-containing complex by immobilizing a hexapeptide corresponding to the C-terminus of the NMDA receptor and subsequent binding of PSD95 along with associated proteins from mouse brain preparations...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
Ngày tải lên :
18/06/2014, 19:20
... 10 mM KHCO and 0.1 mM EDTA in deionized water) was used to remove erythrocytes, and the remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were ... at 37°C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline (PBS) containing mM ethylenediaminetetraacetic acid (EDTA) for 10 at 37°C After washing with ... enrichment methods Spiking Experiments The human colon adenocarcinoma cell line SW620 expressing EpCAM (>99%) and CK (>99%) was cultured in RPMI 1640 containing mmol/L glutamine and supplemented with...
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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
Ngày tải lên :
20/06/2014, 03:20
... 10 mM KHCO and 0.1 mM EDTA in deionized water) was used to remove erythrocytes, and the remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were ... at 37°C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline (PBS) containing mM ethylenediaminetetraacetic acid (EDTA) for 10 at 37°C After washing with ... enrichment methods Spiking Experiments The human colon adenocarcinoma cell line SW620 expressing EpCAM (>99%) and CK (>99%) was cultured in RPMI 1640 containing mmol/L glutamine and supplemented with...
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báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
Ngày tải lên :
11/08/2014, 00:22
... bioconjugating QD-COOH to proteins of increasingly higher pI, using cationic proteins such as cytochrome c and lysozyme However, it was observed that the pI of proteins can play a determining factor in ... analysis by CE-LIF and IgG-CHO coupling A 500 μL mixture containing approximately mg/mL rabbit anti-human albumin IgG and sodium meta periodate dissolved in 0.1 M sodium acetate buffered saline was incubated ... reagents and byproducts by dialysis using 100 kDa MWCO microcentrifuge tubes and re-suspended in 1× PBS (pH 7.2) containing dissolved protein The reaction proceeded for hours with gentle mixing The...
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báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx
Ngày tải lên :
11/08/2014, 20:21
... in gunshot injuries:Technology, principal consideration and clinical implementation Head & Face Medicine 2011 7:18 Competing interests The authors declare that they have no competing interests ... conceived of the work and participated in its design and coordination CS and MR made substantial contributions to data acquisation and conception of manuscript CS and MR drafted and designed the manuscript ... the manuscript CW, MRu, NCG have been involved in drafting the manuscript NCG was involved in revising the manuscript All authors read and approved the final manuscript 22 Kucisec-Tepes N, Bejuk...
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Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf
Ngày tải lên :
12/08/2014, 17:22
... nonspecific binding by using fragments (Fab, Fab’, or Fab’2) as capture antibodies and by using proprietary buffer reagents (in addition to the standard casein, BSA, and detergents) to minimize non-specific ... hyaluronic acid binding protein; HSP 60, heat shock protein 60; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; MMP3, matrix metalloproteinase 3; P1NP, procollagen type amino-terminal propeptide; ... possible that our findings cannot be extrapolated to all RA patients; our findings remain to be validated in independent and more diverse cohorts In addition, the fact that RA and control patients...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
Ngày tải lên :
24/08/2014, 09:51
... cross linking Figure represents how phosphoramide mustard forms intrastrand and interstrand cross–links between guanine bases from DNA 23 B Hypothesis and approaches Hypothesis Since ALDH3A1 is involved ... scaffold and facilitates catalysis by linking together the cofactor–binding and catalytic domains through its interaction with Arg–264 and Arg–475 (Larson et al., 2005; Larson et al., 2007) Since ... protein was filtered, and its concentration and specific activity were determined before flash freezing aliquots in liquid nitrogen and storing at −80°C ALDH1A1 and ALDH2 protein was purified and...
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guide for structural maintenance of parking structures
Ngày tải lên :
24/10/2014, 21:59
... required Inspect deck drain function M S a Deck drains: remove debris and clean out drain; b Drain lines: check for leaks and damage; c Inspect joint sealant at top of drain AR A Install area drain ... to and below expansion joints are especially susceptible to deterioration Beam and column deterioration can be controlled by maintaining joint sealant systems and deck surfaces, and by applying ... reducing joint leakage problems and reducing progressive failure Joint sealants can also fail in adhesion, requiring repair or replacement Contraction joints, construction joints, and joints...
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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films
Ngày tải lên :
10/09/2015, 08:41
... Fracture toughness and interfacial adhesion strength of thin films: indentation and scratch experiments and analysis", in CRC Handbook of Nano-Structured Thin Films and Coatings, Vol.1 (Three-Volume ... difference in grain size Figure 9.10 (a) Representing the biased voltage effect on polarization switching behavior, (b) switching by +6 V dc bias, (c) switching by -2 V dc bias and (d) again re-switching ... affecting local phase stability, (b) acting as 10 Chapter sites for domain wall pinning and (c) controlling the domain nucleation To understand the atomistic mechanisms of polarization switching,...
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Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications
Ngày tải lên :
14/09/2015, 12:53
... family and RTN3 is the principal BACE-interacting protein in human brain (He et al., 2004) Two years later, this group mapped the interaction domains mediating the 19 binding between BACE1 and RTN/Nogos ... expressed by oligodendrocyte myelin and neurons The mature OMgp consists of 401 aas, formed by four domains: one cystein-rich N-terminal domain, a leucine-rich repeat (LRR) domain, a serine-threonine ... to neutralize axonal growth inhibitors in myelin in many in vitro and in vivo studies IN- 1 was firstly generated by Schwab et al using the rat CNS myelin NI250 protein, a fragment of NogoA, as...
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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx
Ngày tải lên :
10/12/2013, 18:15
... connecting and protecting outgoing line powering pairs in ONU (optical network unit) host cabinets • DLC remote terminal OSP pair applications Compact solution for all OSP pairs leaving DLC cabinets ... • Meets all standards for uncontrolled environments including salt-fog, rain, fungus, airborne contaminants and aging Also meets standards for environmental cycling for impulse and AC surges ... leaving DLC cabinets Saves space in the end chamber by providing cross-connect and protection in one field — up to 100% space savings in certain applications • Cabinet overlay applications Compact...
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Fundamentals of quantum mechanics for solid state electronics, optics c tang
Ngày tải lên :
17/03/2014, 14:41
... definition the corresponding integral and it is an ordinary number As far as the final numerical result of the integral is concerned, the information on what coordinate system is used in carrying ... confirmed by substituting (2.24) into (2.16) and making use of (2.19) Solution of the time-independent Schro¨dinger equation is, thus, the first step in solving the timedependent Schro¨dinger equation ... ‘‘commutation relationship’’ and the ‘‘uncertainty principle’’; and the concept of ‘‘eigen values and eigen functions.’’ Commutation relations and the uncertainty principle An interesting question that...
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