study of the interfacial area of protein surface contact by nuclear magnetic resonance spectroscopy
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 11 ppsx
... cations The surface coverage of the clay by the protein can be deduced from the fraction of Mn 2ϩ released The knowledge of both the quantity of protein adsorbed and the surface coverage of the solid ... possible unfolding of the protein on the surface changing the interfacial area between individual protein and surface and the quantity of protein adsorbed at saturation; (3) the surface coverage ... interfacial area occupied by adsorbed proteins Two spectroscopical approaches that permit such investigations are now discussed A Study of the Interfacial Area of Protein- Surface Contact by Nuclear Magnetic...
- 22
- 424
- 0
Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
... forms the floor of the active site The C1 of 6PG is placed near to the cofactor-binding domain with the phosphate directed to the loop between a10–a11 and the tail domain of the partner subunit There ... Fig 6B) The mean atomic B-factor of the PEX ⁄ PEA combination ˚ ˚ (13.3 A2 ⁄ 15.2 A2) is less than the overall B-factor ˚ 2) of the protein The mode of binding of (19.0 A PEX ⁄ PEA in the active ... Occupancy of the ligand and 0.3, respectively Subsequent chemical analysis of the PEX sample by MS (data not shown) identified the presence of PEA, a contaminant carried through from the synthesis of...
- 12
- 452
- 0
Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... 1) Subsite mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and ... during which the protein was eluted at 0.6 m of NaCl Analysis of PelB by gel ltration resulted in a peak with an estimated mass of 212 kDa, corresponding to results of SDS PAGE analyses of the unboiled ... resulted in the overproduction of the 50-kDa PelB, which was veried by SDS PAGE analysis The enzyme was puried to homogeneity by heat treatment of the cell-free extract, followed by anionexchange...
- 10
- 592
- 0
Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... forming contacts with other core complex proteins A further step in the assembly of the secretion apparatus would be the recruitment of components forming the inner membrane pore of the secretion ... of the Cag-T4SS, together with potential implications for the current understanding of its mode of action The cagPAI and the Cag type IV secretion apparatus Gene arrangement and variants of the ... details of secretion apparatus assembly, as well as in the molecular mechanisms of effector protein secretion This minireview describes the composition of the cagPAI and the properties of both...
- 10
- 393
- 0
Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitrogen of Asn114Â of the other subunit The contacts of the ribityl chain to His89 and Lys138Â are mediated by a net of water ... protein atoms made by the hydroxyl groups of the ribityl chain in the other structures Moreover, several direct contacts with the protein chain by the nitrogen atoms and keto groups of the purinetrione ... isotherms for the inhibitors (F) The lled circles in the binding isotherms represent the experimental values of the heat change at each injection; the continuous lines represent the results of the...
- 15
- 439
- 0
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... interpreted as the first evidence for a restricted velocity in the translocation step of the loaded transporter by the sterical conformation of the substrate The introduction of polar side groups in the ... transporter to the internal membrane side is the rate-limiting step and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane ... addressed the question of whether the substrate affinity depends on extracellular pH, by determining the apparent Km values of glycine and alanine at pH values of pH 5.5–8.5 (Fig 4) and a broad range of...
- 8
- 541
- 0
Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt
... indicated by thick black arrows The small arrows indicate the position of the primers used for sequencing by gene walking FW48A corresponds to the first primer used, starting at the known region of the ... processivity of Cel48C was obtained after a 48-h digestion of ASC and Avicel with Cel48C The products of digestion were analysed by TLC by loading both the supernatants of the reaction and the insoluble ... degradation of cellopentaose produced both cellobiose and cellotriose As in the case of ASC, analysis of the kinetics of cellotetraose digestion showed the presence of minor amounts of cellotriose...
- 7
- 404
- 0
Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
... cast on the functional significance of the size variation of the TatA oligomers, because mutant TatA proteins form such oligomers even in the absence of Tat-specific protein translocation [15] The ... productive protein translocation Several of the mutated precursor proteins associate strongly with the membrane in the absence of efficient export It is possible that the proteins are associating with the ... terms of the size of the TatC-containing substrate-binding complex Some of the difference may stem from the absence of a TatB component, but the complexes may well contain differing numbers of TatC-containing...
- 12
- 445
- 0
Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... tetramer The four individual active sites of the tetramer are indicated by the bound CoA molecules The catalytic site of the yellow domain is marked by an arrowhead (B) The binding mode of CoA to the ... of the compound in the structure, further detailed analysis of the exact binding mode has not been performed, but the mode of binding of the acetyl moiety is clearly different from the mode of ... crotonyl-oxyCoA: the hydration rate of the oxoester by crotonase is 330-fold lower [15] Due to different resonance properties of the thioand oxoesters [16], the pKa of the a carbon of a thioester...
- 13
- 472
- 0
Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
... in the preferential binding of the diastereomers to the negatively charged outer surface of bacteria The role of secondary structure formation in cell selectivity was further demonstrated by ... K4L3l4W in the SDS micelles (Fig 4D) Importantly, the positions of the leucine and lysine side-chains emphasize the amphipathic organization of the peptides The relative orientations of the side-chain ... containing the peptides, blue shifts in the In this set of experiments the depth of penetration of the peptides into lipid membranes was estimated through quenching of the tryptophan fluorescence by bromine,...
- 12
- 409
- 0
Báo cáo khoa học: "Metaphor Comprehension - A special mode of language pptx
... the elements of the GCS are themselves m e t a p ~ s in that they contain implicit semantic mismatches These metaphoric elements are interpreted as metaphor inputs thereby making the comprehension ... which the GCS-TKS connections are fully interpreted, that is, more fully integrated The paper discusses the conditions necessary for the strategy to be employed CONCLUSIONS The final part of the ... group, etc.) The comprehension of metaphor does not require a special set of processes to be developed, although the comprehension strategies may be specific to the comprehension task Rather, a metaphor...
- 2
- 311
- 0
Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... analysis of the peptide-binding sites on IN The structure of the PFV intasome in the presence and absence of several drugs served as the basis for the structural analysis [71] The sequences of PFV ... values of the enzymatic reaction, as inferred from the shift of the plots while the same slope was maintained (Fig 2AC and Table S2) These results further suggest that these peptides enhance the ... observed in several of the panels of Fig is the optimum point of the reaction, namely the ideal concentrations of DNA, peptide and amount of enzyme (IN) This may slightly vary with the different peptides...
- 15
- 343
- 0
Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... with the nucleobases pointing towards the protein surface The 5¢-to-3¢ polarity of the ligand follows that of most other nucleic acid complexes of OB-fold proteins, starting in the vicinity of ... conformation of dT6 oligonucleotides in the binding site, the unstacking of bases with respect to each other upon binding, and the shielding of the nucleobase head groups by the protein suggest that the ... vice versa The swapped chains can be interrelated by a noncrystallographic twofold rotation axis The functional units align well with the structures of the two models of the closed protein ˚ (1C9O)...
- 15
- 333
- 0
Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... addition of the peptides to the liposomes, the entrapped calcein (70 mm) was released into the buffer by lysis This relieves self-quenching of the dye within the liposomes, increasing the fluorescence ... and then kill the target micro-organism by interacting with and permeabilizing the cytoplasmic membrane To further study this hypothesis, we examined the ability of PFPs and PCPs to depolarize the ... residue molecular mass of the peptide, c is the concentration of the sample in mgÆmL)1, and l is the optical path length of the cell in cm The spectra were plotted as the molar ellipticity [h]...
- 15
- 376
- 0
Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... exo-activity in the hydrolysis of 1,3-b-oligoglucosides, the subsite theory of Hiromi [46,47] was employed for construction of a subsite map for the estimation of affinities and number of subsites in the ... according to [35] The influence of pH and temperature on activity of the enzyme was studied by the glucose oxidase method The effect of pH on activity was measured at 37 8C for 10 in the range pH 3–9 ... for G3G3G For the corresponding tetraoses the difference was < 4000-fold The mode of action of the T viride exo-1,3-b-glucanase ð1Þ where Km and Vmax are the kinetic parameters of the exo-1,3b-glucanase-catalyzed...
- 9
- 554
- 0
Car Ownership and Mode of Transport to Work in Ireland* potx
... variables The effect of workplace population density also differs across the four areas In general, those resident in rural areas behave differently to those resident in the other three areas of the ... regarded as the reference category) We also include a See the Appendix for discussion of alternative formulations of the travel time variable Further details on the construction of the time and ... kilometres of a rail station (for the Dublin city and county and commuter samples, the cut-off is 100 per cent due to the smaller size of the EDs) Using ArcGIS software, data from the An Post...
- 34
- 476
- 0
Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... molecular target of GE23077 is not the r subunit itself, the fivefold lower activity and the different shape of the inhibition curve observed in the absence of r indicate that the presence of this factor ... E coli [21] The similarity in the mode of action of the two antibiotics, together with the observation that GE23077 is active on rifR RNAP mutants, raises the question of what is the resistance ... RNAP, the possibility was investigated that the compound might inhibit RNA synthesis by preventing RNAP from binding to DNA Binding of RNAP to DNA is indeed one of the earliest steps of the transcription...
- 9
- 339
- 0
Báo cáo Y học: Exploring the primary electron acceptor (QA)-site of the bacterial reaction center from Rhodobacter sphaeroides Binding mode of vitamin K derivatives pptx
... unambiguously clear, the positions of the protons in the protein binding site were automatically assigned by FLEXX In cases of ambiguities (hydrogens of the hydroxyl groups, the N-bound proton of the histidine ... the middle of the quinone rings and the center of the special pair (defined as the middle of the line connecting the Mg atoms) Due to different methyl substitution patterns the use of the edge-to-edge ... geometry) The values of DG0 translat and DGrot Nrot consider the loss of translational and rotational freedom of the entire ligand molecule and the freezing of rotational degrees of freedom of the...
- 13
- 534
- 0
stenersen - chemical pesticides - mode of action and toxicology (crc, 2004)
... understand properly either the mode of action of the pesticides at the target site or their uptake, distribution, and degradation in the ambient environment The development of resistance of various pests ... malathion The increased rate of oxidation to the active toxicants, the oxons, is suggested as one of the mechanisms, and the level of CYP enzymes is elevated Figure 2.8 shows the effect of the herbicide ... Mode of action and toxicology Dose and effect The law of mass action tells us that the amount of reaction products and the velocity of a chemical reaction increase with the concentrations of the...
- 296
- 396
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008