... conformation.DatabaseStructural data are available in the protein Data Bank under the accession number3OQS.Structured digital abstractlCLIC4 and importin alpha bind by x-ray crystallography ... reflection data file was then passed through a suite of programs in the ccp4 crystallography package [36].Scaling of intensities and inspection of data quality wereperformed in scala. In particular, ... dimensions of approximately a =7 9A ˚, b =9 0A ˚and c = 100 A ˚(see Table 1). The final diffraction datasetwas obtained over 180 images with a 1° oscillation angle(u) and 5-s exposure time per image....
... nonoxidative decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox-ylase. J Am Chem Soc 129, 9278–9279.20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a penta-coordinate mononuclear metallocofactor. J Am ... model of cerebral malaria, a frequently fatal com-plication of Plasmodium falciparum infection; in thesame model, pharmacological reduction of PA levelswas demonstrated to correlate with a better...
... Westland, pp xvi-xvii The Project Management Life Cycle Page 3 of 10 AEW Services, Vancouver, BC â2007 Email: max_wideman@sfu.ca ã A list of the alternative solutions available; ã An analysis ... a step by step guide to the nine critical management processes: time management, cost management, quality management, change management, risk management, issue management, procurement management, ... Meeting the quality expectations of the customer can be a challenging task. To ensure that the quality expectations are clearly defined and can reasonably be achieved, a quality plan is documented....
... alpha-sarcin: a study of relationships. Meth Enzymol 341,335–351.3 Lacadena J, A ´lvarez-Garcı´ a E, Carreras-Sangra N,Herrero-Gala´n E, Alegre-Cebollada J, Garcı´ a- OrtegaL, On˜aderra ... Nucleic AcidsRes 4, 1097–1110.2 Martı´nez-Ruiz A, Garcı´ a- Ortega L, Kao R, LacadenaJ, On˜aderra M, Manchen˜o JM, Davies J, Martı´nez delPozo A & Gavilanes JG (2001) RNase U2 and alpha-sarcin: ... were analyzed in a semi-automated iterative mannerby cyana 2.1 [38]. The NOE coordinates and intensitiesused as input for automated analysis were generated auto-matically by Sparky based on...
... The fact that EDTAeffects a marked stabilization (Table 1), however, indi-cates an additional destabilizing effect by calcium ions.Destabilization of PLDa2 by calcium ions was alsoindicated ... in the absence and presence of NaCl and CaCl2at pH 5.5 and 23 °C are compared.The results show that the instability of PLDa2isBC A DFig. 4. Spectra of native PLDa2. (A) UVabsorbance spectrum. ... modified Scatchard plot of these data, with DA being the change in relativeactivity (Fig. 7C), shows two linear parts, indicatingthat at least two separate calcium-binding events affectPLDa2 activity. A BFig....
... that protein family.In addition, we show that both its fold and potentialsurface partially resemble the structural features of theantimammalian scorpion a- neurotoxins and of the humanantibacterial ... theinactivation of the Na+channel in a fashion similar to that of scorpion a- toxins [11], other experiments have shown that, atlow doses, it has an analgesic activity involving both centraland ... triple-stranded antiparallel b-sheet wasFig. 3. Summary of data used in the determination of the secondary structureof crotamine. (A) Primary sequence of Crt with the sequential andmedium-range...
... a ccordance with thePacific Institute of B ioorganic Chemistry Policy o n HumanCare and Use of Laboratory Animals.ResultsIsolation and characterization of lipid A fromM. vagaExtraction of ... for Bioorganic Research, Osaka, JapanThe c hemical s tructure ofa novel lipid A, the major com-ponent of the lipopolysaccharide from the marine gamma-proteobacterium Marinomonas vaga ATCC 27119T,wasdetermined ... endotoxin antagonism have a disaccharide or monosaccharide backbone, mainly onephosphate group, and low degree of acylation [8–10].Lipid A acylation patterns, which are important inbinding bacteria...
... 5Â-aataatccatggggagtaatacttcctggcgtaaaagtgaagtcc-3Â and 5Â-attattggatccctattgctggtccgattctgccag-3Â.3-TPR NlpI primers (residues 62197) were 5Â-aataatccatgggggcacagcttttatatgagcgcggag-3Â and 5Â-aataatggatcctcactgttccttatccgatttttcgaagtgc-3Â. ... the sharp curvature of the array andreduced superhelical pitch, leading to the formation ofa globular structure. Helix a Residues Deviationb,c A AB pair WcAB WcAAÂRotationd(AB) (A BÂ)Helix ... chemically syn-thesized by the W. M. Keck Core Facility (Yale University,New Haven, CT, USA). Primers to amplify mature NlpI(residues 20294) were 5Â-aataatccatggggagtaatacttcctggcgtaaaagtgaagtcc-3Â...
... perframe.Native data were collected to a resolution of 1.4 A ˚. A total of 360 frames of data with an oscillation angle of 0.45° were collected. The exposure time was 0.5 s perframe. All datasets ... Frederick CA, Wang AH & Rich A (1987) A bifurcated hydrogen-bonded conformation in thed (A. T) base pairs of the DNA dodecamerd(CGCAAATTTGCG) and its complex with distamy-cin. Proc Natl Acad Sci ... &Tucker PA (2005) Auto-Rickshaw: an automated crystal structure determination platform as an efficient tool forthe validation of an X-ray diffraction experiment. ActaCrystallogr D 61, 449–457.61...
... purification, characteriza-tion and structural determination of charybdin, a novel29-kDa protein from bulbs of C. maritima agg.Charybdin was characterized by biochemical methodsand its structure ... Access to Research InfrastructureAction FP6 program.References1 Gemmill CL (1974) The pharmacology of squill. NewYork Acad Med Bull 50, 747–750.2 Narayanan S, Surolia A & Karande AA (2004) ... characterization and cry-stallization of charybdin. The yield of the purified pro-tein was 150–200 mg protein per 100 g of bulbs.Charybdin appeared as a single band with a molecularmass of...
... on Innovative ap-plications of artificial intelligence, AAAI’97/IAAI’97,pages 598–603. AAAI Press.Michael Collins and Terry Koo. 2005. Discrimina-tive reranking for natural language parsing. ... Processing of the AFNLP, pages 522–530, Suntec, Singapore, Au-gust. Association for Computational Linguistics.Canasai Kruengkrai, Kiyotaka Uchimoto, Jun’ichiKazama, Yiou Wang, Kentaro Torisawa, and ... Proceed-ings of ACL-08: HLT, pages 897–904, Columbus,Ohio, June. Association for Computational Linguis-tics.Wenbin Jiang, Liang Huang, and Qun Liu. 2009. Au-tomatic adaptation of annotation standards:...
... cruciallimitation to the cytotoxicity of dimeric RNases.AbbreviationsBS-RNase, bovine seminal RNase; ds-RNase A, domain-swapped RNase A; FAM-AUAA-TAMRA, 6-carboxyfluorescein-dArU(dA)2-6-carboxytetramethylrhodamine; ... Germany).FAM-AUAA-TAMRA was from Metabion InternationalAG (Martinsried, Germany). All other chemicals were of the purest grade commercially available.Expression, renaturation and purification of RATEsThe ... be active [48–69%,relative to monomeric RNase A, with RNA as sub-strate; 1–5%, relative to monomeric RNase A, with6-carboxyfluorescein-dArU(dA)2-6-carboxytetramethyl-rhodamine (FAM-AUAA-TAMRA)...
... site 3 of Na+channels. A total of 50 nonredundant sequences of Na+channels available in databases were aligned withCLUSTAL X[45]. The segments S5–S6 of domain I and S3–S4 of domain IV from ... binding because of the absence of primaryelectrostatic attraction [70]. Cn12 has a slightly negativecharge at physiological pH, and the neuroblastoma cellsFig. 7. Multiple alignment of amino-acid ... purification and amino-acidsequence determination of Cn12. (A) A sample of fraction II-4 from a CM-cellulose ion-exchange column [33] containing 1 mg proteinwasappliedtoananalyticalC18reverse-phasecolumn...
... completion ofa project". So, broadly speaking what we have (as happens so often) is a variety of labels that essentially mean the same thing. Just that some terms are more comprehensive than ... Page 7 of 7 AEW Services, Vancouver, BC â 2005 Email: max_wideman@sfu.ca Summary As author Jason Charvat notes in the introduction to his book, there are not many publications that address ... technical goals of scope, quality and customer satisfaction as well. Together these are the marks ofa truly successfully project! This is a valuable book for the insight it provides into a variety...
... Marta Andriantsiferana2, Terumi Nakajima1and Takashi Iwashita11Suntory Institute for Bioorganic Research, Mishima-Gun, Osaka, Japan;2Faculty of Science, University of Antananarivo,MadagascarThe ... obtained with a polyadenylation signal (AATAAA) in the 3Â untranslatedregion and a poly (A) tail at the 3Â end. The open readingframe of IsTX encodes a precursor of 63 residues, contain-ing a ... 5Â-GTXCAYACXAAYATHCCXTG-3Â;3SP2,5Â-AAYATHCCXTGYMGXGGXAC-3Â;3SP3,5Â-GAYTGYTAYGARCCXTGYGA-3Â.5Â RACE: 5 SP1, 5Â-GACAGAAATTACATTTTCGTAGCGT; 5SP2, 5Â-CCATGGACAATTGTTGTAGCAGTT-3Â;5SP3,5Â-TGCCTATTCATACATTTTGCCCT-3Â. The PCR products were cloned into thepCR2.1 vector and the DNA...