... amideoxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto what is observed in VPRK. Both PRK and VPRKhave calcium bound at Ca3. SPRK also ... within a relatively narrow range(pH 5–8) and are often used in the food industry.Alkaline peptidases are generally highly active aroundpH 10, have broad substrate specificity and are activeat ... subtilases is capable of accommodating at least sixamino acid residues (P4P2Â; notation according toSchechter and Berger [14]) ofa polypeptide substrateor inhibitor [15], and both main chain and...
... 6,501–523.50 Feller G, Payan F, Theys F, Qian M, Haser R &Gerday C (1994) Stability and structural analysis of alpha-amylase from the antarctic psychrophileAlteromonas haloplanctis A2 3. Eur J Biochem ... glycosylase fromAtlantic cod (Gadus morhua) reveals cold-adaptationfeatures. Acta Crystallogr Sect D 59, 1357–1365.10 Aghajari N, Van Petegem F, Villeret V, Chessa JP,Gerday C, Haser R & Van ... side chain and carbonyl oxygen of Asp9, the side chains of Asp12, Gln13, Asp19, the car-bonyl oxygen of Asn21 and one water molecule in a pentagonal bipyramidal manner (Fig. 4A) . Sequencealignments...
... Significantly, this structural feature of NOXtp is highly similar to that of valosine-containingprotein-like ATPase from Th. acidophilum, an archaealmember of the AAA family (ATPases associated ... theutilization of oxygen by Desulfovibrio gigas. Eur J Bio-chem 216, 443–448.32 Sakuraba H, Takamatsu Y, Satomura T, KawakamiR & Ohshima T (2001) Purification, characterization,and application ofa ... with a native molecularmass of 300 kDa. A ring-shaped hexameric form was revealed by electronmicroscopic and image processing analyses. NOXtp catalyzed the oxidiza-tion of NADH and NADPH and...
... cerevisiaeMAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase(MAPKK) and a MAPKK kinase (MAPKKK), arecommon signalling modules in eukaryotic ... the unavoidable nonbiological variations accompanying manyexperiments, it is imperative to consider a way of unravelling the functionalinteraction structureofa cellular network (e.g. signalling ... cellu-lar network structure based on stationary experimentaldata, which is applicable to a network of generalizedmodules under the assumption that each module con-tains at least one intrinsic parameter...
... HinCel 6A and CcCel6C (Fig. 2)indicated that Asp150 and Asp334 of CcCel6C arethe potential catalytic residues and could act as a proton donor and a base, respectively. Another aspar-tic acid ... Igarashi3,Masahiro Samejima3, Kiyoharu Fukuda1, Atsushi Nishikawa2and Takashi Tonozuka21 Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Japan2 ... hydrolase family 6enzyme, CcCel6C, a cellulase constitutively produced byCoprinopsis cinereaYuan Liu1, Makoto Yoshida1, Yuma Kurakata2, Takatsugu Miyazaki2, Kiyohiko Igarashi3,Masahiro...
... organicrinorganichybrid materials due to their applications in catalysis,sorption, energy storage, molecular electronics, optical ma-wxterials and ceramics 1–4 . A promising synthetic routetakes advantage ofa ... synthesized and structurally characterized. The2102 310 structure of title compound consists of infinite chains running parallel to the b axis, that are made up of distorted WO octahedra linked6through ... low-temperature soft approach, hy-drothermal method, and the structure- directing function of organoamines. Recent developments in this area haveproven its viability for the successful preparation of...
... mature a bacterial class I c-type cytochrome, Paracoccus denitrif-icans cytochrome c550[33]. Moreover, many taxa thathave heme lyase apparently have separate heme lyasesfor the maturation of ... compilation ª 2009 FEBS 2831 of the alanine of the AXXCH motif (Ala25) (ingreen) and the unsaturated vinyl group of the heme(cyan) are separated by 3.41 A ˚(as compared with a typical thioether ... Trypanosomatidae and theglycosomal redox balance of insect stages of Trypanoso-ma brucei and Leishmania spp. Mol Biochem Parasitol149, 155–169.16 van Hellemond JJ, Simons B, Millenaar FF &Tielens...
... Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki,Japan2 Research Institute of Genome-based Biofactory, National Institute ... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal-ysis of the mode of action of Xgh7 4A has not beenperformed, Xgh7 4A appears to be an endoglucanasebecause ... Prism (GraphPad Software, San Diego,CA, USA). One unit was defined as the amount of enzymethat released 1 lmol of glucose equivalent as reducing sug-ars from xyloglucan per minute.Analysis of substrate...
... enzyme activity: crys-tallographic structure at 2 -A resolution of cephalospo-rinase from the ampC gene of Enterobacter cloacae P99and comparison with a class A penicillinase. Proc NatlAcad Sci ... molecu-lar replacement method, based on the structureof theclass C b-lactamase from E. cloacae P99 (Protein DataBank code 2BLT) as a search model. The model wassolved to a resolution of 2.2 A ˚. ... University of Lie`ge, Institute of Chemistry B 6a, Lie`ge-Sart, Tilman, Belgiumb-Lactamases are the major causes of bacterial resis-tance to the b-lactam family of antibiotics, such aspenicillins...
... D36N-15-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3D341N-15-AAGGGGAGAACTCAACGGAACACCGGAGG-3D389N 5-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-35-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3M. Karlstroăm ... 5’-CCTGGAGAAATCGATCATGAGCTTCGCTCAGTCGTG-3’5’-CACGACTGAGCGAAGCTCATGATCGATTTCTCCAGG-3’F205M 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N ... inhydrophobic area and a 1.2% decrease in charged areacompared with the closed HcIDH (Fig. 4B, Table 2). A BFig. 4. (A) Distribution of hydrophobic, polar and charged accessiblesurface area (ASA) of...
... Ragona L, Molinari H, Tava A & Zetta L(2003) Anticarcinogenic Bowman-Birk Inhibitor Isolatedfrom Snail Medic Seeds (Medicago scutellata): Solution Structure and Analysis of Self Association ... Statistics for the total amount of experimentaldata are reported in Table 3. A simulated annealing (SA) procedure was usedstarting from a randomly generated linear polypeptidechain. The actual ... respectively, bymeans of standard Lineweaver–Burk analysis of initial ratekinetics. Fitting of the experimental data required as inputdata the initial concentrations of BApNA, enzyme andinhibitor,...
... chrysosporium (GenBank: AAA19802),T. reesei (GenBank CAA49596), A. niger (GenBankAAF04491), H. grisea (GenBank AAD11942) and A. acule-atus (GenBank BAA25183) gave sequence identity values of 65%, 64%, ... – a mean for fun ctiona l classification of cellulases. Eur. J.Biochem. 258, 200–206.68. Takashima, S., Iikura, H., Nakamura, A. , Hidaka, M ., Masaki,H. & Uozumi, T. (1998) Isolation of ... against a black background are amino acids that are identical or have a conservedsubstitution in all five sequences. Residues in white against a grey background are amino acids that are identical...
... surface areas and hydrogen bonds of the SAK dimer models. Accessible surface areas are calculated with a probe radius 1.4 A Êaddedto the van der Waals rad ius.Dimer modelBuried surfacearea ... designated asaa, h eadtail, and b±b.Thea a dimer h as a diad3and ischaracterized as helix-helix packing between the twomonomers, as shown in Fig. 2A. The head±tail dimer isformed by a crystallographic ... Processing of X-ra y dirac-tion data collected in oscillation mode. Methods Enzymol. 276,307±326.26. Navaza, J. (1994) AMORE: an automated package for molecularreplacemen t. Acta Crystallogr. A5 0...
... and anadditional C-terminal capping helix (A cap). The onlyway for molecules AB–AB–AB A capto arrange on‘head-to-tail’ packing is if the C-terminal A cap-helix isdisplaced to allow B 3A1 Â ... Thesequences of the first, second and third A- helices of CTPR390are as follows: first A- helix, AEAWKNLGNAYYK; second A- helix, ASAWYNLGNAYYK; and third A- helix, AKA-WYRRGNAYYK. The B-helix sequence in all ... 127–134.11 Fraczkiewicz R & Braun W (1998) Exact and efficientanalytical calculation of the accessible surface area andtheir gradient for macromolecules. J Comput Chem 19,319–333.12 Kajander...