structure function relationships of sp b

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... a phosphate of ribose diphosphate is stabilized by interactions with the x nitrogen of the guanidino group of residue R403 and the c carbonyl oxygen of residue N404 The 3¢-phosphate is stabilized ... an absorbance of 0.8 at 600 nm, expression of EcODC was induced by adding 0.5 mm isopropyl thio -b- dgalactopyranoside After 10 h of growth at 30 °C, corresponding to an absorbance at 600 nm of ... decarboxylase of E coli A B Fig Stereo views of the binding sites of EcODC for ADP (A) and PADP (B) The 2F0 ) Fc electron density of the ligands is contoured at 2.5 r Hydrogen bonds are shown as black...

Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

Danh mục: Báo cáo khoa học

... equation (Ipyr, indolepyruvate, Pyr, pyruvate, Bf, benzoylformate) Substrates Ipyr Pyr Bf 4-NO2-Bf 4-Cl-Bf 4-Br-Bf 4-F-Bf 4-C2H5-Bf 4-CH3-Bf 4-CH3O-Bf 20 3381 1646 48 19 617 111 127 1043 Km (relative) ... analysis of substrate speciﬁcity and cofactor binding of EcIPDC The enzymatic conversion of all substrates studied in this work (pyruvate, the native substrate of PDC, benzoylformate, the native substrate ... However, a weak substrate excess inhibition (Ki 164 ± 16 mM) was observed for pyruvate decarboxylation Examination of the decarboxylation of indolepyruvate by ScPDC and ZmPDC The ability of ScPDC and...

Structure function relationships of variegin a novel class of thrombin inhibitors

Danh mục: Cao đẳng - Đại học

... peptides by thrombin 106 3.3.2 Inhibition of thrombin amidolytic activity by cleavage product, MH22 110 3.3.3 The inhibitory constant Ki of MH22 113 3.3.4 Inhibition of thrombin amidolytic activity by ... removal of backbone kink 178 Table 4.11 Thrombin inhibitory activity of DV23 and DV23K10R 179 Table 4.12 Optimization of thrombin-s-variegin interactions: Cterminal VAla22 substitution 183 Table ... 1HRT) (B) Haemadin-thrombin complex (PDB: 1E0F) (D) Boophilin-thrombin complex (PDB: 2ODY) (C) Ornithodorin-thrombin complex (PDB: 1TOC) E F (E) Rhodniin-thrombin complex (PDB: 1TBQ) (F) Triabin-thrombin...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

Danh mục: Báo cáo khoa học

... MLL1: a structure function perspective A Cleavage site Break point AT-Hooks CXXC PHD BD FYRN FYRC Win SET TAD 1- -3969 Cleavage by Taspase HCF 1SET Menin -3969 DPY30 Ash2L WDR5 CBP MOF RbBP5 B Fig ... portion of the MLL1 TAD bound to the KIX domain of the cAMP response element-binding (CREB) binding protein 2879 3762 -3969 3770 MLL1 TAD domain MLL1 Win motif c-Myb WDR5 CBP-KIX domain (CBP) [23], ... compilation ª 2010 FEBS M S Cosgrove and A Patel MLL1’s interaction to the KIX or CREB-binding domain of CBP [31] The KIX domain of CBP is a structural platform that is capable of binding several...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): structure–function relationships docx

Danh mục: Báo cáo khoa học

... FEBS Z.-C Ding et al Structure reactivity function study of GIF A1 B1 A2 B2 Fig (A1) and (A2) Simulated structure of the b- domain of rlMT2 (B1 ) and (B2 ) Predicted structure of the b- domain of ... N-terminus might contribute to the dynamics of the b- domain of hGIF However, the structure of the b- domain of hGIF remained unsolved by NMR spectroscopy because insufﬁcient amounts of medium-range and ... or the a-domain of hMT1g [the b( MT3)-a(MT1) mutant] [23] It was found that the metal-binding ability and solvent accessibility of the Cd3S9 cluster of the b- domain of the b( MT3) -b( MT3) mutant decreased...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

Danh mục: Báo cáo khoa học

... subtype IC50 (nM) Method ˚ rmsd/resolution (A) 4/7 4/7 4/7 4/7 4/7 4/7 4/7 4/7 4/6 4/6 4/6 4/3 4/3 a 3b2 a a7, a 3b2 a a7, a 3b2 a a 3b2 , a 3b4 a a 3b2 b a7, a 3b2 , a 4b2 a a 6b2 b3, a 3b2 a a 3b2 , a7a a 3b4 a a 3b4 a ... Disulﬁde bond isomers of a-conotoxins (A) A schematic representation of the globular, ribbon and beads isomers possible in any of the a-conotoxins (B) The three-dimensional structure of native AuIB ... (ribbon and beads) forms were less active and more ﬂexible than the native globular isomer The ribbon connectivity of AuIB has been synthesized and although the overall structure appears to be...

Báo cáo khoa học: Evolutionary changes to transthyretin: structure–function relationships ppt

Danh mục: Báo cáo khoa học

... the site of RBP synthesis, and the secretion of RBP into the blood is initiated by the binding of retinol In the bloodstream, RBP is bound to transthyretin with afﬁnities in the range of 1.0 · ... eight b- strands, named A through H, that are connected by loops to form a sandwich of A 3D structures of other transthyretins B Fig The 3D structures of human transthyretin Ribbon diagrams of (a) ... is believed to be the most important distributor for T4 in the blood of humans [41] because of its association and dissociation rates for TH that are between those of albumin and thyroxine-binding...

Báo cáo khoa học: Structure ⁄function analysis of spinalin, a spine protein of Hydra nematocysts doc

Danh mục: Báo cáo khoa học

... amino acids of the protein [3] Secondary structure of spinalin The conformational state of spinalin was analyzed by CD spectroscopy and ﬂuorescence spectroscopy The CD spectrum of spinalin in ... Structure ⁄ function analysis of spinalin S Hellstern et al ture ⁄ function analysis of full-length native spinalin A structure- based homology screen revealed a similarity of the predicted spinalin ... Journal compilation ª 2006 FEBS Structure ⁄ function analysis of spinalin S Hellstern et al network of disulﬁde links In the case of spinalin, subdomain I shows a pattern of several GYGG repeating...

Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx

Danh mục: Báo cáo khoa học

... (2000) Fluorescently labeled neomycin as a probe of phosphatidylinositol-4,5-bisphosphate in membranes Biochim Biophys Acta 1464, 35–48 Cabrera C, Gutierrez A, Blanco J, Barretina J, Litovchick ... percent of inhibition of 12G5 binding to the cells was calculated by the formula: 100 ) [(A ) B ⁄ C ) B) · 100]; where A is the MFI obtained in the presence of APACs and 12G5 mAb, B is the MFI obtained ... b- mAb only (55.56) c- mAb + µM Neam-r6 (9.6) d- mAb + 10 µM Neam-r6 (3.13) a c d b a- Isotype control (4.0) b- mAb only (55.56) c- mAb + µM Neo-r6 (14.8) d- mAb + 10 µM Neo-r6 (4.39) a d c b...

Báo cáo khoa học: Structure–function analysis of the ﬁlamentous actin binding domain of the neuronal scaffolding protein spinophilin pot

Danh mục: Báo cáo khoa học

... regions of disorder in the ABDs of spinophilin and neurabin As shown in Fig 1, these programs also predicted a high degree of disorder in the ABDs of spinophilin and neurabin On the basis of these ... of the spinophilin and neurabin PDZ [22] and neurabin SAM [27] domains have been solved recently by NMR spectroscopy Spinophilin interaction with F-actin is regulated by phosphorylation of its ... portions of human neurabin-1 (HsNEB1), human spinophilin (HsNEB2) and rat spinophilin (RnNEB2), calculated using six different prediction software programs Random coil predictions (%) APSSP2 HsNEB1...

Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt

Danh mục: Báo cáo khoa học

... i.e branching at the b- carbon atom classically considered to destabilize a-helices because of steric clashes The side chain of leucine differs from valine, isoleucine and threonine by having a branch ... absence of the branched b- carbon This may explain why the oligomerization and kinetic properties of V106L are the same as those of V106M [18] and other enzymes of the tetrameric group II (Table ... cells [43–45] Ó FEBS 2004 Eﬀect of amino acid 106 on human TK1 (Eur J Biochem 271) 2255 The observations described above [42] extend our model [21] by showing that phosphorylation of TK1 is involved...

Báo cáo khoa học: Protein tyrosine phosphatases: structure–function relationships ppt

Danh mục: Báo cáo khoa học

... (through the N-SH2 b sheet deﬁned by strands bD¢, bE and bF) but its phosphopeptidebinding site (deﬁned by the loops EF and BG) is not functional; by contrast, binding of a phosphopeptide to the ... Binding of an allosteric inhibitor of PTP 1B keeps the catalytic WPD-loop in the open conformation Structure of PTP 1B (cyan) in complex with the allosteric inhibitor 3-(3,5-dibromo-4-hydroxy-benzoyl)-2-ethyl-benzofuran-6-sulfonic ... both structural genomics (see above) and structure- based drug design efforts The state of structure- based development of membrane-permeable RPTP inhibitors (a focus of the pharmaceutical industry)...

Báo cáo khoa học: Structure–activity relationships of fowlicidin-1, a cathelicidin antimicrobial peptide in chicken docx

Danh mục: Báo cáo khoa học

... average structure of fowlicidin-1 (B) Stereo-diagrams of the backbone trace of the 20 lowest energy structures of fowlicidin-1, with residues 8–16 overlaid (C) Stereodiagrams of the backbone trace of ... ﬁnal structures against an averaged structure resulted in a rmsd ˚ value of backbone of < 0.5 A (Table 1) Greater ﬂexibility between the helices was revealed when only one 2584 I23 Hyd ro phob ic ... solution structure of the active domain of CAP18- a lipopolysaccharide binding protein from rabbit leukocytes FEBS Lett 370, 46–52 32 Terwilliger TC, Weissman L & Eisenberg D (1982) The structure of...

Báo cáo y học: "What does the structure-function relationship of the HIV-1 Tat protein teach us about developing an AIDS vaccine?" ppsx

Danh mục: Báo cáo khoa học

... and Tat Bru; both of which have been shown to be biologically active in the absence of reducing agents Therefore, it is probable that the different folding observed in the NMR study of Tat Z2 ... distribution, increasing the blood brain barrier (BBB) permeability [73] Tat also exerts a neurotoxic effect on hippocampal neurons by disinhibiting Ca2+-permeable N-methyl-D-aspartate (NMDA) receptors ... locomotion by engaging the alpha beta and alpha v beta integrins and by mobilizing sequestered basic fibroblast growth factor Blood 1999, 94:663-672 Barillari G, Gendelman R, Gallo RC, Ensoli B: The...

STRUCTURE-FUNCTION ANALYSIS OF CXXC FINGER PROTEIN 1

Danh mục: Y khoa - Dược

... methyl-CpG-binding domain (MBD) (Jorgensen 2004) Binding of methyl CpG binding proteins physically obstructs the basal transcription machinery (Hattori 2004) Mbd1 is a member of the subfamily of MBD ... binding to each has been shown to be inhibited by methylation (Singal 1999) Methylation of the promoter of the neurofibromatosis gene, NF1, interferes with Creb and Sp1 transcription factor binding ... absence of Cfp1 by clonogenic survival assays DNA damage accumulation was analyzed by accumulation of H2AX-γ by Western blot analysis and platinum incorporation onto DNA by atomic absorption spectroscopy...

Structure function studies of vesicle associated membrane protein associated protein b (VAPB) associated with amyotrophic lateral sclerosis (ALS

Danh mục: Kỹ thuật

... properties of the wt-VAPB MSP domain 39 3.1.2 Stability of the wt-VAPB MSP domain 41 3.1.3 Binding activity of the wt-VAPB MSP domain 41 3.1.4 Crystal structure of the wt-VAPB MSP domain 42 Studies ... consequence of the Thr46Ile mutation 52 3.3.2 Residue specific conformational properties of VAPB (T46I) 52 3.3.3 Stability of VAPB (T46I) MSP domain 54 3.3.4 Binding activity of VAPB (T46I) MSP domain ... model of VAPB based on the nematode MSP dimer structure (PDB: 1MSP) As shown in figure 2, the Proline residue at position 56 of VAPB creates an S-shaped loop between two short stretches of β-strand...

Comparatives study on sequence structure function relationship of human short chain dehydrogenases reductases

Danh mục: Tổng hợp

... groups of human SDRs 53 Figure 13: The location of the conserved and variable residues in the template structure of group of human SDR was identified by Talana 54 LIST OF TABLES Table 1: PDB ... sequences of human SDR enzymes were collected from NCBI-BLAST (http://blast.ncbi.nlm.nih.gov/) Human SDR protein structures were collected RCSB Protein Data Bank (PDB) (http://www.rcsb.org/pdb/home/home.do) ... individual specificity highlights the more variable regions By investigating both types of specificity, our results may be of better use for future work on developing inhibitors that can be directed...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

Danh mục: Báo cáo khoa học

... limits of detection of the experimental procedures Addition of phosphate or phosphite restored phosphorylase activity, as shown in Fig 5A ,B, respectively The time course of formation of phosphate ... phosphorylase II Mode of binding of pyridoxal 5Â-phosphate and analogs of pyridoxal 5Â-phosphate to apophosphorylase b and the aggregation state of reconstituted phosphorylase proteins Biochemistry 11, ... (1994) A study of binary complexes of Escherichia coli maltodextrin phosphorylase: a-D-glucose 1-methylenephosphonate as a probe of pyridoxal 5Â-phosphatesubstrate interactions Biochim Biophys Acta...

Tài liệu Báo cáo khoa học: Second messenger function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) doc

Danh mục: Báo cáo khoa học

... the plasma membrane and intracellular membranes (reviewed in [5]), rapid increases of [Ca2+]i can Second messenger function of cADPR be achieved by increasing the open probability of Ca2+ channels, ... (FKBP 12.6, calstabin2) was found to bind cADPR and to mediate responsiveness of RyR towards cADPR [35,36] The data support a model in which binding of FKBP 12.6 to RyR decreases its open probability, ... whereas binding of cADPR or FK506 to FKBP 12.6 weakens the interaction between FKBP 12.6 and RyR, thereby resulting in an increased open probability of RyR Other studies have shown that in speciﬁc...

Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx

Danh mục: Báo cáo khoa học

... shows the structure of BDD (C) Eect of substrate (BAEE) on the time course of inactivation of ChC by BDD (pH 7.6, 25 C) No BDD (h); BDD, 16.97 lM (w); BDD, 16.97 lM in the presence of mM BAEE (r) ... to caspases towards the C terminus 990 N E Labrou and D J Rigden (Eur J Biochem 271) ể FEBS 2004 Fig Determinants of P1 substrate specicity in (A) clostripain (specic for Arg) (B) caspase (specic ... [12] Our database searches using PSI-BLAST [24], in both GenBank and Unnished Microbial Genome databases at the NCBI (http://www.ncbi.nlm.nih.gov/blast/), initially located, ignoring obviously partial...

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