structure function and dynamics channel gating and desensitization
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... myelopoiesis and organogenesis [8-11] Both SDF-1-deficient and CXCR4-deficient mice die perinatally and have very few hematopoietic stem cells and progenitors within their bone marrow SDF-1 and CXCR4 ... 5'-GCTGCTGCTGT-GGCAAAGT-3' and reverse 5'AGCCTGACAGTGACCAGG-3' ; and actin, forward 5'ACCCAGATCATGTTTGAGAC-3' and reverse 5'-GTCAGGATCTTCATGA-GGTAGT-3' A relative standard curve method was used ... The standard curve was made from six points corresponding to 10-fold cDNA dilution series For each sample, the relative amount was calculated from its respective standard curve Standards and samples...
- 10
- 386
- 0
Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
Ngày tải lên :
12/08/2014, 17:21
... data and statistical analysis, and drafted and revised the manuscript HS and CA assisted with the acquisition of data and analysis RS performed mRNA expression analysis with microarrays YS and ... treated and recruited the patients for this study, and analyzed the clinical data of the patients TO and NN made substantial contributions to the conception and design of the experiments, and analysis ... into functionally related gene groups and rank their importance, was performed EASE classified the gene categories into three GO systems: biological process, cellular component, and molecular function...
- 9
- 302
- 0
Báo cáo khoa học: "Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity" doc
Ngày tải lên :
07/08/2014, 23:22
... irradiation After 24 h, the supernatants were harvested and used for the determination of IL-12 and TNF-alpha, the representative cytokines of cell-mediated and innate immunity The cytokine concentration ... concentration of × 10 cells/ml in 6-well plates and treated with fucoidan (50 μg/ml) for 24 h and then irradiated once (1 Gy) After 24 h, the cells were harvested and stained for flow cytometric analysis ... cultured and treated as previously described in flow cytometric analysis section The cells were harvested and then used for Western blot analysis The lysates of treated BMCs were obtained and the...
- 7
- 255
- 1
Báo cáo y học: "Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold" docx
Ngày tải lên :
09/08/2014, 06:22
... Food and Drug Administration These polymers are favorable for the synthesis and secretion of a cartilaginous matrix, such as proteoglycans and R557 type II collagen, and act as a physical and ... decalcified in EDTA, and embedded in paraffin Sagittal sections (5 µm thick) were prepared from the center of the defect area and stained with hematoxylin and eosin, alcian blue, and safraninO–fast ... C, 38.8 ± 16.9% in group T-4, and 76.5 ± 18.7% in group T-12 (Fig 3) Significant differences in the %MM were seen between C and T-12 (P = 0.007) and between T-4 and T-12 (P = 0.007) The average...
- 8
- 355
- 0
Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx
Ngày tải lên :
13/08/2014, 05:21
... BRL, Grand Island, New York, USA), polybrene (6 μg/ml) (Sigma, Saint Louis, USA) and an antibiotic mixture (5 mg/ml penicillin; mg/ml streptomycin; 10 mg/ml neomycin; Gibco BRL, Grand Island, New ... early-immediate promoter and enhancer region The 5' half of the env gene (nt 6582 to 7981) was also removed, leaving the RRE (REV-responsive element) sequence and the 5' and 3' exons of the tat and rev regulatory ... irradiation and transplantation on polymorphonuclear cell, lymphocyte and thrombocyte counts Effect of4 Effect of irradiation and transplantation on polymorphonuclear cell, lymphocyte and thrombocyte...
- 15
- 330
- 0
báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
Ngày tải lên :
21/06/2014, 02:20
... visualize and quantify nanostructures of them AFM images included single and multiple BMDCs, two and three dimensions, low and high resolutions, cell height profile and histogram, topography, and roughness ... comparison and a P value of less than 0.05 was considered statistically significant Results and discussion Morphologic and functional characteristics of BMDCs The bone marrow cells were cultured and ... immature and mature bone marrowderived dendritic cells (BMDCs) Obviously, this study would provide a novel insight into the nanostructure and force feature of immature and mature DCs Materials and...
- 9
- 469
- 0
Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... contributions YL, DZ and YZ carried out the main experiment and drafted the manuscript FZ and DZ conceived of the study and designed the experiment GH helped to finish animal model and finished statistical ... experimental protocols were approved by the Animal Care and Use Committee of Nanjing Medical University and were in compliance with Guidelines for the Care and Use of Laboratory Animals, as published by ... mg/kg, intraperitoneally) and the right femoral artery was dissected free along its entire length All branches were ligated and excised The left hindlimb was kept intact and used as the nonischemic...
- 10
- 230
- 0
báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging" ppsx
Ngày tải lên :
11/08/2014, 00:22
... design, collection and assembly of data, performed the statistical analysis, interpretation and drafted the manuscript ALMT, HMPN and JAP assisted with collection and assembly of data and performed ... weekly and evaluated after 1, 7, 14 and 21 days of culture (B) The number of MSCs labeled after and 21 days of culture without trypsinization (C) Mitomycin-pretreated cells labeled with Feridex and ... data and helped draft the manuscript ELG conceived of the study, participated in its design and coordinated its execution DCS, ACCC and RMO conceived of the study, participated in its design and...
- 13
- 256
- 0
Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... blood examination and liver and renal functions, no manifestations of acute and chronic toxic reactions and graft versus host disease, no local swelling at injection sites, and no lower limb ... acute and chronic toxic reactions and graft versus host disease were observed during and after MSC transplantation Meanwhile, the body temperature, routine blood parameters and liver and renal functions ... of liver and renal functions and lymphocyte subset Their results revealed that intravenous transplantation of MSCs was safe and feasible Devine et al intravenously injected autologous and allogeneic...
- 10
- 584
- 0
báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
Ngày tải lên :
18/06/2014, 15:20
... AL, DN, and JP carried out the 2D electrophoresis and all other experimental work PS, ET, and WKH coordinated the laboratory work and helped to draft the manuscript All authors read and approved ... patient D and spots No 91 (Ku antigen) and 115 (Rho A) were not present in patient C (Figure 3) Furthermore, only patients F and G showed an expression of plasminogenactivator inhibitor-2 and a variant ... marrow and peripheral blood cells of selected individuals (Table 2) Spot No (14-3-3 related) was only present in patient C, spot No 121 and 122 (TCHTP and variant) was only present in patient F and...
- 8
- 529
- 0
báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx
Ngày tải lên :
18/06/2014, 15:20
... 30, 2008, and Food and Drug Administration (FDA) recently promulgated regulations on human cells, tissues, and cellular and tissue-based products [2] issuing an appropriate regulatory structure ... makes reference to and is in coherence with the 2004/23/EC directive on donation, procurement and testing of human cells and tissues and with directive 2002/98/EC on human blood and blood components ... regulations pertaining to biologic products and human cells, tissues, and cellular and tissue-based products provide an appropriate structure for ensuring the safety and efficacy of the next generation...
- 9
- 773
- 0
Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
Ngày tải lên :
18/06/2014, 16:20
... GK Collection and assembly of data AD Collection and assembly of data AK Data analysis and interpretation, collection and assembly of data CP Conception and design, data analysis and interpretation ... Conception and design, provision of patients, data analysis and interpretation, manuscript writing PA Conception and design, data analysis and interpretation, manuscript writing HA Data analysis and ... reduced number of functionally competent cells [17] Therefore, formation of myocytes and coronary vasculature cannot counteract the chronic loss of functional cells and Page of vascular structures [5]...
- 5
- 411
- 0
báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... VEGFR-2, and vWF, induced MSCs and EPCs functioned normally, as indicated by both fine crystals of ossification (comparable to natural bone mineral) and ALP activity in osteoblasts, and capillary-like ... for cell process and spreading, adhesion and orientation Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and appeared better viability and strong cellular ... polymer membranes and methods of preparation of three dimensional membrane structures 1996 Yu H, Matthew HW, Wooley PH, Yang S-Y: Effect of porosity and pore size on microstructures and mechanical...
- 9
- 337
- 2
Báo cáo y học: "Osteoclast-independent bone resorption by fibroblast-like cells" doc
Ngày tải lên :
09/08/2014, 01:21
... were seeded on the dentine slices and cultured in Dulbecco’s modified Eagle’s medium with 10% FCS and 2% penicillin/streptomycin, with and without TNF-α (10 ng/ml and 100 ng/ml) Dentin slices were ... After 60 days, mice were sacrificed and the implants removed Tissue preparation included fixation in 4% buffered formalin and paraffin embedding according to standard procedures Sections (4 µm) ... ligaments and the menisci were excised, whereas the posterior cruciate ligaments were preserved The articular cartilage and the most superficial bone were resected from the tibial condyles and a...
- 11
- 251
- 0
Báo cáo y học: "Bone marrow lesions from osteoarthritis knees are characterized by sclerotic bone that is less well mineralized" ppsx
Ngày tải lên :
09/08/2014, 01:22
... analysis and interpretation, and manuscript preparation, and approved the final manuscript LG, TAE, and RAM participated in data analysis and interpretation, manuscript preparation and approved ... indicates an ideal cylindrical rod structure, an SMI of indicates an ideal plate structure, and values less than zero indicate a structure in which the plates are curved and begin to close off the pores ... acquisition of the data, data analysis and interpretation, and manuscript preparation, and approved the final manuscript MF and SJ participated in acquisition of the data and manuscript preparation Acknowledgements...
- 9
- 368
- 0
Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
Ngày tải lên :
09/08/2014, 07:20
... the design of the study and the analysis of the data DN participated in the design of the study and the analysis of the data, and wrote the manuscript All authors read and approved the final manuscript ... 100 U/ml penicillin and 100 µg/ml streptomycin When cultures reached near confluence, cells were detached with 0.05% trypsin and 0.53 mM ethylenediamine tetracetic acid, and were subsequently ... microscopically observed, and passage The median age of the BM-MSC samples was 53.13 ± 18.3 years, corresponding one-half to healthy donors and approximately one-quarter to RA patients and one-quarter...
- 12
- 342
- 0
Báo cáo y học: "Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 07:20
... designed the study, and participated in experimental procedures, collection, analysis, and interpretation of data, and manuscript preparation YM and NC contributed to analysis and interpretation ... fibroblastlike cells and on the production of MMP-1 and VEGF was evaluated by Wilcoxon's signed rank test Correlation between serum C-reactive protein and NFκB1 mRNA in bone marrow CD34+ cells and that between ... SCF (10 ng/ml) and GM-CSF (1 ng/ml) After 24 hours of incubation, chemical transfection of siRNAs was performed, and incubated for hours Cell cultures and measurement of MMP-1 and vascular endothelial...
- 10
- 412
- 0