structure defects and properties of the finished casting
Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... sheet through the C-terminal loop is conserved Structure of the FMN-binding region The hydrogen bonds and salt bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic ... repeat and relative expression of the HD gene We then determined the structure of the mouse CB1 gene and determined that transcription of the CB1 gene was affected in striatal neurons of transgenic ... levels in the medial striatum of any of the wild-type and HD mice examined The optical density of the hybridization signal in the lateral striatum, corrected by subtracting the signal in the medial...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are ... on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in the presence of saturating levels of ... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the papers can be eluted to isolate the individual sugars which can then ... catalytic mechanism of TreS, each of the radioactive substrates was incubated with the enzyme in the presence of high concentrations of the unlabeled product, and the formation of radioactive product ... at times up to 22 h Properties of the TreS purified from M smegmatis Effect of time and protein concentration on formation and characterization of the products The conversion of trehalose to maltose...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... hydroperoxides and aldehydes These may be the primary substrates of the microsomal or membrane-bound GST in the same way as they are the substrates for Se-dependent glutathione peroxidases (the ‘classical’ ... Using the standard curve constructed from the protein standards (Fig 4B), the molecular mass of the Peak GST subunit was determined to be 34.0 kDa (Table 2) Peak showed subunits of 36.8 and 32.6 ... used and pH was confirmed immediately prior to and following the assay; the pH values shown are the average of these two values Peak (d, lines) and Peak (s, dotted lines) GST had pH optima of 7.20...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... as control The reaction rate was calculated from the slope of the absorbance curve, using software installed by the manufacturer (Beckman Coulter) The extinction coefficient under the conditions ... using standard conditions described in the text Results are expressed as the percentage of activity with no addition of solvent Standard error for all experiments is less than 5% of the value ... toward medium and long chain esters of pNP Maximal reaction temperature is 55 °C and LipA shows strong activity in the presence of metals, inhibitors, and organic solvents Based on these properties, ...
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báo cáo khoa học: " Preparation and properties of the specific anti-influenza virus transfer factor" pot
... present the results of the methods of preparing and analyzing the specific transfer factor oral preparation in vitro experimental system The data indicated that the methods were credible and the ... pertaining to the range of normal TF (Table and Figure 1, 2) And the ratio of A260 to A280 was up to the criteria of National Health Department on transfer factor Contents of polypeptides and nucleotides ... contain the essence of the immunological message Ultraviolet spectrum analysis The analysis of TF preparations in ultraviolet spectrum absorption indicated that the highest peak of specific and non-specific...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... noting the switch of Leu13 from being part of the exposed hydrophobic groove in the top-top structures to becoming part of the hydrophobic core in the top-bottom structures and assuming the role of ... of 24 structures, the lowest energy and the best (smaller rmsd from the mean) structure of RIIa D/D Each monomer of RIIa D/D consists of a disordered N-terminal segment Fig Superposition of the...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... structure and properties of K137E and the K137,141E mutant of human HSP22, aiming to provide new information on the structure of sHsp and to shed new light on their role in the development of human ... However, the overall structures of aB-crystallin and HSP27 predicted by these two models are very similar, and the positions of the b-strands correlate well with the corresponding positions of the ... sHsp The primary structure of the a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding loop connecting the b5 and...
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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the ... with the remainder of the target residues assigned by detection of NOESY residueheme and interresidue cross-peaks solely by the standard Mb fold, and the observation of relaxation effects and/ or...
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Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
... present structures are characterized with local materials, namely, “Tufo of Matera” for the structures of the "Sassi of Matera," and “Stone of Fasano” for the structures of the "Trulli of Alberobello," ... characteristics of the "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites of the "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... rocky church crypt [3], the energetic behavior and the microclimate of different hypogeous structures in the site of the "Sassi of Matera" [4, 5], and the thermal behavior of different opaque envelopes...
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Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
... smaller The RA and the PA sub-domains had lower correlations with their domains than most of the other domains at baseline, at endpoint, and Psychometric properties of the CHIP-CE also for the change ... the SH sub-domain (item 1: ‘‘How often does your child have a lot of fun?’’ and item 4: ‘‘How often does your child feel happy?’’) The items of the SPS sub-domain Psychometric properties of the ... as well as for the domains and sub-domains Furthermore, the sub-domains were correlated with the domains and the total score, and the domains were correlated with the total score The items/sub-domains/domains...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1 (such as downstream of v-KIND knockdown...
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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... by presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1 If the duration of the stimulus exceeds the critical value, the trajectory in the (si, s1) ... by number in the plane of the Csk and RPTP concentrations Within these two regions of monostability, there are parameter sets where the QSS dependence of the active Src fraction on the inactive ... transition from the Off state to On state (in the bistability domain) Regardless of the slope, the active Src fractions increase during the Off to On transition, whereas the value of the inactive...
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... regions and the entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone The open reading frames of the five ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... c the Soret band was reported to absorb at 418 nm, the b-band at 520 nm and the a–band at 550 nm [18] The deviations of approximately nm between the signals of horse heart cytochrome c and the ... at 415 and 442 nm, and for the oxidized form at 403 and 422 nm The b–band can be seen at 517 nm and the a–band at 547 nm and 603 nm The difference signals that can be observed between 400 and 700 ... work we study the electrochemical, UV-VIS and FT-IR spectroscopic properties of the caa3oxidase from T thermophilus in the presence and absence of cyanide, and compare the observed properties to...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... complexes of GGH, where the a carbon of the His residue is the sole source of chirality [10] However, while positions of the component dd bands and of CT transitions are relatively constant, their ... albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained These data can be very useful in further studies of the toxicologically ... below) The comparison of CD spectra of complexes also points toward slight differences in the conformation of the chelate rings The characteristic alternate pattern of the dd bands in the CD...
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Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
... understanding and will be the focus and repeating theme throughout the text A sound understanding of cardiac physiology begins with basic understanding of cardiac anatomy and of the physiology of ... contraction and instantaneous atrial volume) The pressure of the point at the bottom right corner of the loop is the pressure in the ventricle at the end of the cardiac cycle and is called the end-diastolic ... ongoing contraction process of the cardiac muscles and the properties of the arterial system and is beyond the scope of this lecture As the contraction process of the cardiac muscle reaches its...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... for the determination of the structures of the conformers of the C-domain The dipolar couplings provided long-distance information on the global folding of both conformers The structure of the ... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal of the HN of Gly337 in the open conformer and the signal of the water protons All of these observations indicate that the loop region of the open conformer has a higher degree of...
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