structure composition and properties of the bc1 complex
Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
Ngày tải lên :
07/03/2014, 02:20
... Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing of the avoredoxin ... His126 of A fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivative, although a ferric ion was not found at the site of either...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ... description of recent advances in the understanding of the channel-, motor- and regulatory components of the Tic complex For reference, some of the available knowledge, including the proposed function of ... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
Ngày tải lên :
23/03/2014, 20:22
... exhibiting the bands of the remaining oxy complex The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading 600–700 nm The latter ... maximum of the Soret band with a small absorption coefficient (Table 1) On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the visible ... which is monitored by the steady decrease of the Soret and 498 nm bands and the shift of the band at 630 nm to the longer-wavelength direction At the same time, a broad band with the maximum at approximately...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
Ngày tải lên :
16/03/2014, 18:20
... determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic ... repeat and relative expression of the HD gene We then determined the structure of the mouse CB1 gene and determined that transcription of the CB1 gene was affected in striatal neurons of transgenic ... levels in the medial striatum of any of the wild-type and HD mice examined The optical density of the hybridization signal in the lateral striatum, corrected by subtracting the signal in the medial...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
Ngày tải lên :
17/03/2014, 10:20
... recorded in the spectra were mapped onto the anticipated sequence of subunit C on the basis of their mass value and the specificity of the enzyme, leading to the complete verification of the amino ... parallel the effect of the absence of other homologous regulatory subunits of oxygenase complexes, like T4MOD [33] and component B of methane monooxygenases [38,39] In these cases the absence of the ... soluble fraction of the induced cell in the case of the expression of pET22b(+)/touBEA and /touC, whereas the product of the expression of pET22b(+)/touF was accumulated in the insoluble fraction,...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
Ngày tải lên :
17/03/2014, 10:20
... describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are ... on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in the presence of saturating levels of ... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
Ngày tải lên :
23/03/2014, 13:20
... trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the papers can be eluted to isolate the individual sugars which can then ... catalytic mechanism of TreS, each of the radioactive substrates was incubated with the enzyme in the presence of high concentrations of the unlabeled product, and the formation of radioactive product ... at times up to 22 h Properties of the TreS purified from M smegmatis Effect of time and protein concentration on formation and characterization of the products The conversion of trehalose to maltose...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
Ngày tải lên :
23/03/2014, 15:20
... hydroperoxides and aldehydes These may be the primary substrates of the microsomal or membrane-bound GST in the same way as they are the substrates for Se-dependent glutathione peroxidases (the ‘classical’ ... Using the standard curve constructed from the protein standards (Fig 4B), the molecular mass of the Peak GST subunit was determined to be 34.0 kDa (Table 2) Peak showed subunits of 36.8 and 32.6 ... used and pH was confirmed immediately prior to and following the assay; the pH values shown are the average of these two values Peak (d, lines) and Peak (s, dotted lines) GST had pH optima of 7.20...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
Ngày tải lên :
23/03/2014, 21:20
... as control The reaction rate was calculated from the slope of the absorbance curve, using software installed by the manufacturer (Beckman Coulter) The extinction coefficient under the conditions ... using standard conditions described in the text Results are expressed as the percentage of activity with no addition of solvent Standard error for all experiments is less than 5% of the value ... toward medium and long chain esters of pNP Maximal reaction temperature is 55 °C and LipA shows strong activity in the presence of metals, inhibitors, and organic solvents Based on these properties, ...
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Báo cáo y học: "Composition and conservation of the mRNA-degrading machinery in bacteria" pps
Ngày tải lên :
10/08/2014, 05:21
... RNA-binding properties, and Page of 12 therefore can potentially modulate the efficiency and/ or specificity of the degradosome-mediated RNA decay More significant differences in the composition of degradosomes ... PNPase, phosphofructokinase and enolase [13] Further characterization of these complexes and elucidation of their specific roles in mRNA decay in B subtilis and related species can offer many important ... conserved and can be absent in some classes of bacteria [6,7] The availability of new genomic data and discovery of novel RNases in bacteria prompted us to re-assess the phylogenetic conservation of these...
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báo cáo khoa học: " Preparation and properties of the specific anti-influenza virus transfer factor" pot
Ngày tải lên :
11/08/2014, 20:20
... present the results of the methods of preparing and analyzing the specific transfer factor oral preparation in vitro experimental system The data indicated that the methods were credible and the ... pertaining to the range of normal TF (Table and Figure 1, 2) And the ratio of A260 to A280 was up to the criteria of National Health Department on transfer factor Contents of polypeptides and nucleotides ... contain the essence of the immunological message Ultraviolet spectrum analysis The analysis of TF preparations in ultraviolet spectrum absorption indicated that the highest peak of specific and non-specific...
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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
Ngày tải lên :
23/03/2014, 17:22
... is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the ... with the remainder of the target residues assigned by detection of NOESY residueheme and interresidue cross-peaks solely by the standard Mb fold, and the observation of relaxation effects and/ or...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
Ngày tải lên :
19/02/2014, 05:20
... pocket structure As shown in Table 2, the hyperfine splitting constants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1 complex are closer to those of the ... analyses of the molecular features of the heme–GmHO-1 complex and of the mechanism of heme degradation were performed, and the results were compared with those for the heme complexes of SynHO-1 and ... The spectrum of the extract is shown in Fig 8B, exhibiting peaks at 404, 534, 636 and 684 nm; the latter two differ from the 660 nm band of the protein complex (Fig 8A) When the solution of the...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
Ngày tải lên :
08/03/2014, 22:20
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... noting the switch of Leu13 from being part of the exposed hydrophobic groove in the top-top structures to becoming part of the hydrophobic core in the top-bottom structures and assuming the role of ... of 24 structures, the lowest energy and the best (smaller rmsd from the mean) structure of RIIa D/D Each monomer of RIIa D/D consists of a disordered N-terminal segment Fig Superposition of the...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
Ngày tải lên :
23/03/2014, 07:20
... structure and properties of K137E and the K137,141E mutant of human HSP22, aiming to provide new information on the structure of sHsp and to shed new light on their role in the development of human ... However, the overall structures of aB-crystallin and HSP27 predicted by these two models are very similar, and the positions of the b-strands correlate well with the corresponding positions of the ... sHsp The primary structure of the a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding loop connecting the b5 and...
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Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
Ngày tải lên :
30/03/2014, 11:20
... in complex with ADP as well as the loops in chains C and D of the Uu-TK–dT structure (B) Superimposition of the nucleotide-binding region in the substrate complex (orange) and the inhibitor complex ... of the phosphate donor in the dNK family, there is a domain in hTK1 and Uu-TK that contains a structural zinc atom and lasso-like loop In the feedback inhibitor complexes of Uu-TK and hTK1, the ... but, in the absence of substrate or feedback inhibitor, the substrate site is open and the lasso-like loop is disordered [16] In the absence of a true substrate complex for any member of the TK1...
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Báo cáo lâm nghiệp: "Short Communication Differences in the structure, species composition and diversity of primary and harvested forests on Changbai Mountain, Northeast China" ppsx
Ngày tải lên :
07/08/2014, 03:22
... to be reconsidered from the perspective of both sustaining timber yields and ecologically sustainable forest management; in the process the structure and composition of the primary forest may ... genera and 13 families were identified Of the overall number of tree species, 21 were present on both the primary and logged forest sites: species of seedlings, 19 species of saplings, 15 species of ... amurensis, F mandshurica, Q mongolica, A pseudo-sieboldianum, A mono and U japonica), which accounted for 63% of all trees and 91% of the total basal area (Tables and 3) These percentages reflect the typical...
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The composition and morphology of amphiboles from the rainy creek complex, near libby, montana
Ngày tải lên :
14/02/2016, 08:55
... Fe2+ and the same analyses plotted with all Fe calculated as Fe3+ The Y-axis represents the amount of Na + K in the A-site of the amphibole structure, and the X-axis the amount of Na in the B-site ... spectroscopy Because of the large range of compositions of the amphiboles, we compared the results of calculating total Fe as Fe2+ vs total Fe as Fe3+ The difference in the handling of Fe made a small ... C-site cations in the B-site would decrease the amount of Na in the B-site and increase the amount of Na in the A-site, thereby affecting the cation distributions and possibly the amphibole species...
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Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
Ngày tải lên :
05/09/2013, 14:58
... present structures are characterized with local materials, namely, “Tufo of Matera” for the structures of the "Sassi of Matera," and “Stone of Fasano” for the structures of the "Trulli of Alberobello," ... characteristics of the "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites of the "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... rocky church crypt [3], the energetic behavior and the microclimate of different hypogeous structures in the site of the "Sassi of Matera" [4, 5], and the thermal behavior of different opaque envelopes...
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