structural organization of a chromosome euchromatic and heterochromatic regions of the drosophila polytene chromosomes
Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx
... (present paper and [16]) both the LH2 and the LH1 antenna systems are present, while in the work of Parkes-Loach et al an LH2–, LH1– strain and an LH2– strain from Rb sphaeroides and from Rb capsulatus, ... prior to the onset of the reaction at its maximal rate In wild-type ICM, the initial rate of this reaction, as well as the lag phase, depends on the redox state of the ubiquinone pool and on the ubiquinone/RC ... 5A, lanes and 3, corresponding to PMC3 from PufXD2)4 and PufXD2)7, respectively, above the dominant LH1 a and b bands, a band attributable to the mutated PufX is clearly visible In lane a faint...
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Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identification of the origin of a new viper population based on ammodytin I1 heterogeneity docx
... CGCGGATCCAATCTTGATGGGGC AGCCGGAGAGG AGGAYTCTCTGGATAGTGG CTCACCACAGACGATWTCC CGGTAAGCCCATAACGCCCA CAGGCCAGGATTTGCAGCC CATAAACAYGAGCCAGTTGCC GAGTGGATGCACAGTCGTTG GAAACGGAGGTAGTGACACAT GCCTGCTCGAATTCGGGATG ... GCCTGCTCGAATTCGGGATG CTCCTTCTTGCACAAAAAGTG CTGCTCGAATTCGGGATG GTCYGGGTAATTCCTATATA GTGATCGAATTTGGGAAGATGATCCA CCCTTGCATTTAAACCTCAGGTACAC a Specific primers used for amplification of the Bov-B LINE retroposon; ... PLA3G1 (R) PLA5G2 (F) PLA3G2 (R) PLA3G4 (R) ARTFa (F) ARTRa (R) AtxBFb (F) AtxBrcb (R) AtxACFc (F) AtxACrcc (R) AmlFd (F) Amlrcd (R) CAGGAAACAGCTATGAC CGGAATTCTGAAGGTGGCCCGCC AGGTGACAG CGCGGATCCAATCTTGATGGGGC...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG L CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC ... STQLPSDEPLNGLNDKAIQDILNEHNMFRAKEHVPPLTWNTTLA 44 Hordeum MQTPKLVILLALAMSAAMVNLSQAQNSP YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP ... Q CAG T ACC Q CAA K AAG P CCG K AAG S TCC H CAC W TGG M ATG ACG C TGC G GGT E GAG T ACC E GAA A GCC L CTC K AAG G GGC Y TAC K AAG I ATC B Cyn d 24 CGT I ATC G GGA A GCG R AGG G GGA I ATA T ACG...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... generated by a crystallographic two-fold The data and refinement statistics are shown in Table The Ramachandran plot has 96.9% of the residues in the most favored region and 3.1% in the additional ... dehydrogenase and prephenate dehydratase catalyze the biosynthesis of tyrosine and phenylalanine, respectively As this biosynthetic pathway is absent in mammals but is essential for the survival of bacteria ... was amplified by PCR using the forward primer 5¢-GG AATTCCATATGCAACCCACTCATACGCTAACAAG-3¢ (with the NdeI restriction recognition sequence underlined) and the reverse primer 5¢-CGGGATCCTTATTTTAATT...
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structural and functional organization of the synapse
... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA, USA and Michael D ... collapse of the vesicular membrane These vesicles are then rapidly retrieved at the site of release, and are immediately available for rerelease (33) At the ultrastructural level, many of the ... for synaptopodin and the spine apparatus in hippocampal synaptic plasticity Ann Anat 2007; 189:5–16 Eyman M, Cefaliello C, Ferrara E et al Local synthesis of axonal and presynaptic RNA in squid...
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Báo cáo y học: " A sequence-based survey of the complex structural organization of tumor genomes" pdf
... used AGGAAAAGGCCTTGAAGCTC and TGCTGTATTTGACAGGACAAGTG (outer primers), and GAGGACATGCTCCTACCTGTG and TGCTGTATTTGACAGGACAAGTG (inner primers) For CN272097 we used CCAACGTGAGCTTCCAGAAC and ACAGAAACGCCTCTTCTCATTTAG ... wrote the paper All authors read and approved the final manuscript 10 Additional data files The following additional data are available with the online version of this paper Additional data file ... variants, clustering of the identified breakpoints, and comparison of ESP and aCGH data SV and CC developed the ESP methodology and BES mapping algorithms, analyzed the data and coordinated the...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... of the approaches described above, heat denaturation and aggregation of BLG upon heat treatment may hide putative sites of attack from the action of proteases, therefore leaving intact some regions ... protein/protease mixture was then placed in a thermostatted water bath for the required amount of time At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity ... confirm that the accessibility of cleavage sites on the substrate, rather than the intrinsic catalytic ability of a given protease, is what limits the effectiveness of the enzyme action on actual protein...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... S The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters would ... It is also clear from comparisons of panels c and d that the hydrolysis rates of the Tyr146-Asn147 and the Asn147-Ala148 bonds were similar and that, indeed, the replacement of Asn147 was also ... fragment II in the degradation of the Tyr146 !His/Asn147 !Ser mutant (panel d) indicated a significant decrease in the rate of cleavage of the autolysis loop At the same time the amount of fragment...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... conserved in all b-amylases from plants and microorganisms (e.g 97Gly-Gly-Asn-Val-GlyAsp-Ala-Val104 of Calystegia b-amylase and 96Gly-GlyAsn-Val-Gly-Asp-Ile-Val103 of the soybean b-amylase) Docking ... grows at the one end and dies at the other end) If so, the C sepium and alfalfa taproot b-amylases represent a unique type of VSP because they are located in the cytoplasm whereas all other known ... orientation The program TURBOFRODO (Bio-Graphics, Marseille, France) was used to calculate the Ramachandran plot and perform the superposition of the models Electrostatic potentials were calculated and...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
... hypophosphatasia, (b) infantile hypophosphatasia, (c) childhood hypophosphatasia, (d) adult hypophosphatasia and (e) odonto hypophosphatasia [1–4] Perinatal and infantile forms of hypophosphatasia are ... GGC TAC AAG ATA GTG GGC GGT GAA-3¢ and 5¢-TTC ACC GCC CAC TAT CTT GTA GCC AGG-3¢; and V406F, 5¢-CCT GGC TAC AAG TTC GTG GGC GGT G-3¢ and 5¢-CAC CGC CCA CGA ACT TGT AGC CAG G-3¢, respectively The ... fraction (the raft) at a similar rate (Fig 3B), further supporting that the folding and assembly process and subsequent intracellular trafficking of TNSALP (V40 6A) are largely normal in the stable...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... Fig The 1652 and 1270 cm)1 bands can be assigned to a- helix in the human PKIb; the 1666 and 1247 cm)1 bands are the characteristic frequencies of the random coil structure Meanwhile, the 1682 and ... Table According to the activity assay, the activity was retained above 95% (data not shown) and the recovery is 24% during the heat-treatment It implied that PKIb was a thermostable protein and ... polyadenylation signal ATTAAA (1018– 1023) and poly (A) tail (Fig 1) The human PKIb protein predicted by the open reading frame is 78 amino acids in length with a calculated molecular mass of 8468.2...
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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
... this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic subunits have been often used as markers for mitochondrial biogenesis [6,31,44,45] The Drosophila a- F1-ATPase ... Fig Structure of the D melanogaster a- F1-ATPase gene Chromosomal location and structure of a- F1-ATPase The gene maps to the 2R arm in D melanogaster In the schematic diagram of the gene structure, ... and the GAGA and Adf-1 elements in the DNA are underlined The translation start codon and the main transcription start point are larger and in bold (B) Mutations either in the GAGA or Adf-1 binding...
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Báo cáo khoa học: A structural overview of the PDI family of proteins docx
... ERp57 and the tip of the calreticulin P-domain Proc Natl Acad Sci USA 99, 1954–1959 Satoh M, Shimada A, Keino H, Kashiwai A, Nagai N, Saga S & Hosokawa M (2005) Functional characterization of thioredoxin ... orientation of the bb¢ domains and significant differences in orientation of their a and a domains The catalytic cysteines (orange) of the °C yeast PDI structure face each other (B) The b and b¢ ... cloverleaf A long C-terminal tail folds back and makes contacts with the a and b¢ domains [10] This capping function of the C-terminal tail may have assisted the successful crystallization and structure...
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Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... 5¢-CCGTGAATTCCACCACTCGCATACCTGTGGG 5¢-CCACAGGTATGAGAGTGTTGCAATTCACGGAATCGAAAGG 5¢-CCTTTCGATTCCGTGAATTGCAACACTCTCATACCTGTGG 5¢-CGGAATCGAAGCGGTCGACGAGTTCCACAGG 5¢-CCTGTGGAACTCGTCGACCGCTTCGATTCCG 5¢-GAAAAGCAAGAGTTACCCCCGCGTTTATGGCACAGGAAG ... 5¢-GGCTTGAGTTCCAGAATGTGGCCATCAATGGAGATAAG 5¢-CTTATCTCCATTGATGGCCACATTCTGGAACTCAAGCC 5¢-AATGGCTTGAGTTCCAGGCTGTGGCCATCAATGGAGATAAGTATC 5¢-ATACTTATCTCCATTGATGGCCACAGCCTGGAACTCAAGCCATTC Combination of primers for N43 5A ⁄ ... 5¢-GAAAAGCAAGAGTTACCCCCGCGTTTATGGCACAGGAAG 5¢-CTTCCTGTGCCATAAACGCGGGGGTAACTCTTGCTTTTC 5¢-GCTTGAGTTCCAGGCTGTGAGCATCAATGGAGATAAGTATC 5¢-GATACTTATCTCCATTGATGCTCACAGCCTGGAACTCAAGC 5¢-GGCTTGAGTTCCAGAATGTGGCCATCAATGGAGATAAG...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... above, emphasizing that the disappearance of the a- helix component is associated with the appearance of the intermolecularly aggregated b-sheet When the correlation was made in the presence of ... PKCf according to Griffiths & Pariente [53], using grams ⁄ 32 Derivation gave the number and position, as well as an estimation of the bandwidth and the intensity of the bands making up the amide ... I¢ regions have been described previously [29,32,54] The fractional areas of the bands in the amide I or amide I¢ regions were calculated from the final fitted band areas It is assumed that the...
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Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt
... fragments The Nogo -A cDNA (designated KIAA 0886) was obtained from the Kazusa DNA Research Institute (KazusaKamatari, Kisarazu, Chiba, Japan) A DNA fragment encoding a 182 residue Nogo -A fragment ... (designated as Nogo -A( 567–748); Fig 1) was generated by PCR with a pair of primers: 5¢-CG CGCGCGCGGATCCACTGGTACAAAGATTGCT-3¢ (forward) and 5¢-CGCGCGCGCCTCGAGCTAAAAT AAGTCAACTGGTTC-3¢ (reverse) A DNA ... NMR characterization of the Nogo -A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo -A protein (A) The domain organization of...
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
... Zn-MT and apo-MT species in the presence of Hg(II), the effect of each of the previous variables was analyzed separately To this end, the titration of Zn7-MT, Zn4-aMT and Zn3-bMT proteins, at pH and ... standard variables (pH of the solution, reaction time, and binding Table Influence of the reaction time (t) and binding ability of the counter-ions (X) on the nature and structural features of the ... data There are several reasons: (a) the presence of different chromophores in the same species including Zn and/ or Hg as metal ions and SCys and/ or Cl– as ligands; (b) the absence of well-established...
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Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx
... activity of the RATEs as compared with RNase A ([16] and Table 1) indicate a negative influence of the tandemization on the catalytic efficiency The recovery of activity after proteolytic cleavage of the ... (Figs and S1) revealed that the linkers not constrain the ability of the RNase A entities to adopt the same orientation in the crystal as monomeric RNase A On the other hand, the decreased activity ... RNase A entities within the RATEs are catalytically active and that the activity decrease [16] is a result of the tandemization Accessibility of the linker of SGRSGRSG-RATE in the presence of RI The...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... Product a- GalpNAcA6OMe( 1a -3) -a- QuipNAc(1-OMe c Product a- Quip3NAlaNAc(1-OMe b AlaNAc 1.29 17.9 1.27 17.9 Most NH resonances of CPS and O-chain polysaccharide were assigned on the basis of their large ... 1324.54 – a ¢ a – a ¢-c a- c – a ¢-c-b a- c-b – – a ¢-c-b -a- c-b (a- c-b)2 a a ¢ – a -c a ¢-c – a -c-b a ¢-c-b – (a -c-b)2 a ¢-c-b -a -c-b – – methylation and CE-ES-MS analyses obtained on both CPS and ... Da (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both CPS and O-chain polysaccharides was present in the form of a primary amine...
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