... localization and I. Birschmann et al. Domain function of Pex1p and Pex6pFEBS Journal 272 (2005) 4758 ê 2004 FEBS 57 Structuraland functional analysisof the interaction of the AAA-peroxins Pex1p and ... parts of the N-terminal fragment and ⁄ or the second cassette;ABDCEFFig. 5. Effects of point mutation of theWalkerA and B motifs of the AAA-cassettes of Pex6p on the morphological appearance of ... Here we confirm and extend earlier studies of these AAA-peroxins and givea further detailed functional analysisof their cassettestructure and interaction.The interaction of Pex1p and Pex6p involvestheir...
... of these structures (Doc. S1 and Fig. S1) indicates thattheir active sites are structurally well preserved and that HP0843, HP0844, HP0845 and HP1291 can beconsidered as orthologues of ThiE, ... reaction of B. subtilis TenA [17] assumes that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favoringthe exit of the aminic group. The subsequent addition of a water molecule and the ... reminis-cent of that of human heme oxygenase-1 [13].In the present study, we present the crystal structure of the HP1287 gene product as well as one of itsmutants (F47Y) and discuss the in vivo role of H....
... Plasticity of S2–S4 specificity pockets of executionercaspase-7 revealed by structuraland kinetic analysis Johnson Agniswamy, Bin Fang and Irene T. WeberDepartment of Biology, Molecular Basis of Disease, ... identification of the small aliphaticbinding region of the S4 subsite of caspase-7. The struc-ture of caspase-7 ⁄IEPD shows only one hydrogen bondbetween the main chain atoms of caspase-7 and P4, and lacks ... assistance duringX-ray data collection. Use of the Advanced PhotonSource was supported by the US Department of Energy, Of ce of Science, Of ce of Basic Energy Sci-ences, under Contract No....
... Annual Book of Standards, Part 31, D3694-78. “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Societyfor Testing and Materials, Philadelphia.4. ... identifications should be based uponmeasurements of actual retention time variations of standards over the course of a day.Three times the standard deviation of a retention time for a compound can be ... containing a concentration of C, in àg/L. APPENDIX A TO PART 136METHODS FOR ORGANIC CHEMICALANALYSISOF MUNICIPAL AND INDUSTRIAL WASTEWATERMETHOD 605—BENZIDINES1. Scope and Application1.1 This...
... know-ledge of the identities and quantities of pollutants and other chemical species inwater, air, soil, and biological systems. Therefore, proven, state -of- the-art techniques of chemical analysis, ... LLC 24 CHEMICALANALYSISOF WATER AND WASTEWATER____________________________________________________24.1. GENERAL ASPECTS OF ENVIRONMENTAL CHEMICAL ANALYSIS Scientists’ understanding of the ... in the evolution of analytical chemistry, characterizedby the development of new and improved analysis techniques that enable detection of much lower levels ofchemical species and a vastly increased...
... Annual Book of Standards, Part 31, D3694-78. “Standard Practices forPreparation of Sample Containers and for Preservation of Organic Constituents,”American Society for Testing and Materials, ... controlprogram. The minimum requirements of this program consist of an initialdemonstration of laboratory capability and an ongoing analysisof spiked samples toevaluate and document data quality. The ... Parts 136.4 and 136.5.1.7 This method is restricted to use by or under the supervision of analysts experiencedin the use of HPLC and GC systems and in the interpretation of liquid and gaschromatograms....
... thesecondary dilution standard be prepared at a concentration of 15 àg/mL of eachinternal standard compound. The addition of 10 àL of this standard to 5.0 mL of sample or calibration standard would be ... controlprogram. The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysisof spiked samples to evaluate and document data quality. The ... tap and distilled water and dry at 105°C for one hour before use.5.2 Purge and trap system—The purge and trap system consists of three separate pieces of equipment: a purging device, trap, and...
... anaccurate measure of the koffparameter in CaM-boundeNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational changerates versus rates of FMNH2formation ... in the nNOS and eNOSflavoproteins (dissociation and association of the4-electron reduced FNR–FMN subunit complex, kon and koffin Fig. 5). Remarkably, the results suggestthat koffis the ... theCaM-free nNOS and eNOS, the rate of FMNH2for-mation appears to be relatively fast and not rate limit-ing, and instead a specific conformational step (koff,dissociation of the reduced FMN...
... Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLPWhat lies beneath: Semantic and syntactic analysis of manually ... the mostbasic of errors: a leading coordinator (#1 and 2) and rules proceeded by unnecessaryfiller words (#3 and 5). Complementary rules#7 and 9 (e.g. VP → [rule]/[rule SBAR] and VP → [rule ... reorderings and deletion of the less informative lexemes.These examples show varying degrees of diffi-culty for the task of automatic reconstruction. Ineach case, we also see that semantic analysis of...
... ratio of 5 : 5 : 0.01 (v/v/v) and sprayed at a flow rate of 2 lLặmin)1.MALDI-TOF MS of LPS and free lipid A wereperformed on a Reflex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF ... spectroscopy (Figs 5 and 6) and assignment of all signals (Table 2) revealed that thecarbohydrate structure of the major component of C. tra-chomatis E LPS consisted of three Kdo- and two GlcpN-residues. ... acid analysis (Table 1) indicated that themajor fatty acids were C14:0, iso-C15:0, C18:0, C20:0 and 3-OH-C20:0.MALDI-MS and ESI-MS analysis As a first step in the structural analysis, LPS and...
... calcium-binding loops of EF-hand proteins, have chemical shifts . 10 p.p.m. forfilled loops andchemical shifts of < 8 p.p.m. for emptyloops [50]. The chemical shifts of CR I–II, Calb I – II and several ... to dimerize and both EF-hands of CR I –II bindcalcium. We conclude that the significant structural and biochemical differences between CR I–II and Calb I–IIare related to a region of poor sequence ... oligomeric states of CR I–II and Calb I–II are an indication of differentorganizations of EF-hands within full-length CR and Calb.CR I–II and Calb I–II may have different structuresCR I–II and Calb...
... 15-40% of Mass 95.75 30-60% of Mass 95.95 Base Peak, 100% Relative Abundance.96 5-9% of Mass 95.173 <2% of Mass 174.174 >50% of Mass 95.175 5-9% of Mass 174.176 >95% but 101% of Mass ... of each internal standard compound. The addition of 10 àL of this standard to5.0 mL of sample or calibration standard would be equivalent to 30 àg/L.7.3.3 Analyze each calibration standard according ... controlprogram. The minimum requirements of this program consist of an initialdemonstration of laboratory capability and an ongoing analysisof spiked samples toevaluate and document data quality. The...
... Annual Book of Standards, Part 31, D3694-78. “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Societyfor Testing and Materials, ... controlprogram. The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysisof spiked samples to evaluate and document data quality. The ... stock standards to avolumetric flask. To each calibration standard, add a known constant amount of one or more internal standards, and dilute to volume with 2-propanol. One of the standards...
... controlprogram. The minimum requirements of this program consist of an initialdemonstration of laboratory capability and an ongoing analysisof spiked samples toevaluate and document data quality. The ... more stock standardsto a volumetric flask. To each calibration standard, add a known constantamount of one or more internal standards, and dilute to volume with hexane. One of the standards should ... Annual Book of Standards, Part 31, D3694-78. “Standard Practices forPreparation of Sample Containers and for Preservation of Organic Constitutents,”American Society for Testing and Materials, Philadelphia.4....
... reaction. Analysis of the effect of the mutations on proteinstability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation atall. The first type of relaxation (structural ... Comparative structures of CMP and UMP, and interactions between the cyto-sine moiety of nucleotide and various sidechains of wild-type CMP kinase. (Upper) Chemical differences between CMP and UMP are ... the distal point of therest of the protein. Such a dipole will have weak inter-actions with the rest of the protein. Thus, if turned off(by removal of Arg and protonation of Asp185), theprotein...