structural analysis of unfolded peptides by raman spectroscopy
Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... properties of its complexes with fatty acids path length cm) of 3.5 mL each: one for the assay and one for the control assay The contents of each cuvette were mixed 5–10 times by gentle inversion of ... at room temperature Two types of experiments were performed (a) Titration of cis-PnA was carried out by the increasing amounts of NaTDC at pH 5.0, in the absence of TLL The assay and control cuvettes ... absence of cis-PnA, the addition of NaTDC led to a blue shift of the kmax of all the lipases tested, except for TLL(W89L) Furthermore, in contrast to what occurred with TLL(W89L), the addition of...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... both of the fluorophores will decrease At a ratio of : 1, only around 28% of the complexes may contain both of the fluorophores, and approximately 72% of the complexes may contain only one of the ... spectra of both GnRH-R–GFP and GnRH-R–EYFP were determined by the spectral scanning program of Leica TSC-SPII confocal system The peak of each emission curve was normalized to a set value of 100 ... associations of molecules of any proteins of interest Acknowledgements We acknowledge Dr Daryl Webb for his excellent technical assistance with confocal microscopy; Professor Hiroto Naora, Professor...
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Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... location(s) of structural transitions This allows precise assignment of specific mechanical changes to specific structural transitions Such assignments are especially important in the study of complex ... recipient of a postdoctoral fellowship from the Jane Coffin Childs Foundation P.M.F is a recipient of an NSF predoctoral fellowship This work was supported by grants from the NIGMS Journal of Biology ... monitored by counts on an avalanche photodiode (APD; EG&G Optoelectronics, Gaithersburg, USA), collected through a confocal pinhole The area of regard of the APD was limited to a region of approximately...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... lM of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 mM of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... mediated by OsDmc1 Renaturation reaction of OsDmc1 (1) without competitor; (2) with 27.5 lM unlabeled of PhiC along with 27.5 lM of rhodamine labeled Phi-C; (3) with 55.0 lM unlabeled of Phi-C ... with 27.5 lM of rhodamine labeled Phi-C; (4) with 27.5 lM unlabeled of M13C along with 27.5 lM of rhodamine labeled Phi-C; and (5) with 55.0 lM unlabeled of M13C along with 27.5 lM of rhodamine...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... hetero-oligomerization of D2L and D2S is of particular interest as it may affect the function of the two isoforms of the receptors, with possible direct consequences on the effect of antipsychotic ... being analysed by Western blot Immunoblotting For Western blot analysis of non-immunoprecipitated samples, 25 lg of membrane protein was resuspended in loading buffer and denatured by incubation ... observation of signal Membranes containing the equivalent of 100 fmol of receptors (as labelled with [3H]spiperone) were incubated with 2.5 nM Eu3+-labelled anti-FLAG Ig, in a total volume of 500 lL of...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... citrine-elongin C by coexpression of elongin B suggest that a conformation change of elongin C induced by binding of elongin B may occur and that this conformational change of elongin C leads ... changes of proteins occurring in living cells The occurrence of FRET can be accurately and finely determined by measuring the reduced fluorescence lifetime of donor proteins in the presence of acceptors ... distances (2–3 nm) between other combinations of terminal ends of elongin B and elongin C, although the effects caused by the binding of pVHL on the 3D structure of the elongin BC complex are not exactly...
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Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot
... Rescue of inactive mutants by half molecules of AC8 in vivo A corollary of the experiments described above involving dominant negative suppression of adenylate cyclase activity is the rescue of inactive, ... Premises for this type of behavior by other members of the ABC family of proteins include the ATPactivated K+-channel discussed earlier, which is comprised of a heterooctamer of four SUR protein ... units of ¯uorescence intensity (A) Cotransfection of CFP/8Tm2 and YFP/8Tm2 (B) Cotransfection of 8NTm1, CFP/8Tm2 and YFP/8Tm2 (C) Cotransfection of CFP/AC8 and YFP/AC8 Ó FEBS 2002 Dimerization of...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF PHYSICS NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgement I would like to extend my deep gratitude towards my supervisor, Associate Professor YAN ... determined from the center of the bead to the edge of the cover glass The real-time extension of the DNA tether can be tracked by magnetic tweezers Figure 1.9 shows the time course of the DNA extension ... chromatin is made up of repetitive and regularly spaced nucleosomes, of which each consists of a core histone left-handed wrapped by 147 bp DNA at 1.67 rounds The further compaction of nucleosomes...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... complex were obtained by in vitro biochemical studies using concentrated, purified enzymes Association of PGK with GAPDH was demonstrated by utilizing gel penetration analysis or by using immobilized ... orientation of two fluorophores, being independent of the strength of protein–protein interactions In the case of very weak interactions as described in this article, rapid association and dissociation of ... cytoplasm were reduced by the coexpression of citrine–GAPDH, but coexpression of PGK did not affect the lifetimes Coexpression of citrine–GAPDH and PGK weakly decreased the lifetimes of cerulean–GAPDH...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... were created by subcloning of the EcoRI–NotI segments of the respective Rluc-tagged CaRs into c-myc-CaR Analogously, HA-CaRD1036-GFP2 and HA-CaRD886-GFP2 were created by subcloning of the EcoRI–NotI ... studies of CaR dimerization Ó FEBS 2002 Homodimerization of CaR in living cells (Eur J Biochem 269) 5085 Agonist-induced rearrangement of the 7TMs in the CaR homodimer? One of the goals of the ... the translation of agonist binding to the ATDs of the family C GPCR homodimer into G-protein coupling of the 7TM)7TM moiety is mediated by another mechanism than that proposed by Kunishima et...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... calculated by dividing the fluorescence intensity of YFP–PHD (535 nm) by the intensity of CFP–PHD (489 nm), both of which were obtained by excitation at 430 nm The fluorescence ratio of the FRET ... reintroduction of extracellular calcium (Fig 6Ab) The receptor-induced level of Ins(1,4,5)P3 in cells not pretreated with Tg was 120 ± 12.8% of the Ins(1,4,5)P3 level induced by the reintroduction of extracellular ... oscillation [20] To determine the physiological role of the calcium dependence of Ins(1,4,5)P3 metabolism, a more detailed analysis of the effects of calcium and the cross-talk between other signaling...
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FUNDAMENTALS of KINEMATICS and DYNAMICS
... CamHarmonic.nb provides analysis of a harmonic cam with oscillating and offset followers • 2DOFfree.nb provides analysis of the free vibrations of a two-degree-offreedom system with damping • 2DOFforced.nb ... found Thus, the analysis of mechanisms is reduced to the analysis of their skeletons The skeleton is represented by a system of connected links The position of each link is identified by an angle ... case of welded links) DOF Thus, the two links connected by a revolute joint have four DOF One can say that by connecting the two previously disconnected links by a revolute joint, two DOF are...
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Atomistic modeling of energetics and dynamics of diffusive and frictional phenomena in c60 graphene based systems
... regimes of surface diffusion in C60/graphene system according to the effect of temperature and rotational degrees of freedom (DOFs) of the admolecule In the case of rotational C60 (the set of arrows ... intervals separated by jump events) even in the presence of rotational DOFs (b) At 40 K, in the absence of rotational DOFs, the C60 admolecule still moves by hopping mechanism (dominated by single jumps) ... travelled by the adsorbate per unit of time [40] The temperature dependence of D is conventionally described by an Arrhenius law The growth mechanism is governed by the energetics and dynamics of the...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... CA, USA) 51 Software Description Source GraphPad Software (La Jolla, CA, USA) • GraphPad Prism version 4.0 • Microsoft Excel software Microsoft (Redmond, WA, USA) • Stata version software Stata ... Sprague-Dawley rats, ovariectomized at weeks of age, were treated with 300 mg of Epimedium standardized preparation per kilogram of body weight at weeks of age by gavage The Epimedium preparation (PSC1929/L01-60/B/Wo ... ethanol The preparation contained 143.3 mg/g of icariin, 0.157 mg/g of icariside I, 18.68 mg/g of icariside II, 0.009 mg/g of icaritin, and 0.122 mg/g of desmethylicaritin, respectively Following...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... doses of testosterone in the presence of estradiol (Fig 11) This increase was due to the conversion of testosterone to estradiol by aromatase in the cells, which was prevented by the addition of ... doses of testosterone in the presence of estradiol (Fig 20) This increase was due to the conversion of testosterone to estradiol by aromatase in the cells, which was prevented by the addition of ... hydroxycortisone in the presence of 50 pM of estradiol for a duration of days Changes of cell culture media were carried out twice on the second and fourth day of incubation Similar to what was...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... responses of genes by hierarchial clustering and correlation analysis of gene expression profiles Hierarchial clustering grouped the range of treatments into two main categories One group consisted of ... the 189 exception of kaempferol, which suggests that the mechanisms of ERβ activation by kaempferol may differ from the rest of the phytoestrogens Positions 3’ of ring B and of ring C may have ... plots of each of these treatments against estradiol was found to be CYP1A1 210 4.3.2 Discovery of up-regulation of CYP1A1 transcription by Epimedium compounds via global gene expression profiling...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... MCF-7 breast cancer cells of pure compounds and for measurement of global estrogenicity of complex mixtures To enable the study of the whole spectrum of SERM activities of Epimedium compounds on ... studies The duration of two studies and the number of metabolites that were examined also differed The rat study reported the levels of total amounts of prenylflavonoids absorbed (sum of conjugated ... concentrations of both unconjugated and conjugated levels of ingested prenylflavonids were monitored The levels of conjugated prenylflavonoids were included to see whether lack of estrogenic activity of...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... Chen, W.Q., Zhang, C.X and Chen, Y.M 2009 Effect of long-term intervention of soy isoflavones on bone mineral density in women: a meta -analysis of randomized controlled trials Bone 44(5):948–953 ... Bioavailability of isoflavones after ingestion of soy beverages in healthy adults J Nutr 136(9):2291-6 Kanaze, F.I., Bounartzi, M.I., Georgarakis, M and Niopas, I 2007 Pharmacokinetics of the citrus ... tissue distribution of estrogen receptors alpha and beta Endocrinology 138(3):863–870 Kuhl, H 2005 Pharmacology of estrogens and progestogens: influence of different routes of administration Climacteric...
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Application of formal and dynamic equivalence to the translation of bị and được into english
... becomes the verb of a passive with an addition of auxiliaries bị and The subject of active sentences is often omitted in passive sentences, or is preceded by the preposition (due to, by) Thản (1977) ... STATEMENT OF AUTHORSHIP Title: Application of Formal and Dynamic Equivalence to the Translation of “Bị” and “Được” into English (Graduation paper submitted in partial fulfillment of the Degree of Bachelor ... Administration of the try – out 27 III.1.6.1 Preparation of the try – out 27 III.1.6.2 Try – out 27 III.1.7 Methods of data analysis 27 vi III.1.8 Results of the...
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