stresses phosphorylation of eukaryotic initiation factor 2 eif2 rapidly reduces protein synthesis which lowers energy expenditure and facilitates reprogramming of gene expression to remediate stress damage c
Báo cáo y học: " Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A" potx
Ngày tải lên :
12/08/2014, 23:22
... reveals connections to actin cytoskeleton and cell cycle progression Mol Genet Genomics 20 06, 27 5 :26 4 -27 6 Cracchiolo BM, Heller DS, Clement PM, Wolff EC, Park MH, Hanauske-Abel HM: Eukaryotic initiation ... hypusine-dependent 19 20 21 22 23 24 25 26 27 28 29 30 host cell mechanism for replication of human immunodeficiency virus type (HIV-1) Biochem Pharmacol 1998, 55:1807-1818 Bevec D, Hauber J: Eukaryotic initiation ... a subset of mRNAs encoding proteins that participate in cell cycle control [27 ,28 ] Its biological roles encompass cancer, maintenance of the cytoskeletal architecture, neuronal growth and survival,...
- 17
- 353
- 0
Báo cáo y học: "On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation" potx
Ngày tải lên :
14/08/2014, 20:22
... 5'-ccaacttcggatcaacaactcc-3'; At3g 525 90, 5'-accttgaccggcaagacca-3' and 5'-taagcctcaacacaagatgaagtg-3'; At4g05 320 , 5'-cacactccacttggtcttgcg-3' and 5'-ggtctttccggtgagagtcttc-3'; At5g66140, 5'-tctcaatcttgttttgactcgagc-3' ... CCG CCT CGA CGC CGG CGT CTA CTC CTG CTT GAA GAC GAG GAT GCA GCC GCG GCT GGA GGC GGG GGT GTA GTC GTG GTT TAA TAC TAG TAT TCA TCC TCG TCT TGA TGC TGG TGT TTA TTC TTG TTT Expected [%] 20 0 Characterization ... 300 20 0 100 0 10 20 30 40 50 60 10 30 100 300 uORF length (amino acids) Number of uAUGs (e) 150 100 50 AAA AAC AAG AAT ACA ACC ACG ACT AGA AGC AGG AGT ATA ATC ATG ATT CAA CAC CAG CAT CCA CCC CCG...
- 20
- 350
- 0
Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
Ngày tải lên :
07/03/2014, 15:20
... Ray, B.K., Thach, R.E & Merrick, W .C (1987) The ATP-dependent interaction of 17 18 19 20 21 22 23 24 25 26 27 28 29 eukaryotic initiation factors with mRNA J Biol Chem 26 2, 3 826 –38 32 ´ Pause, A., ... (Invitrogen) to create the constructs pBS-Ubx and pBS-Cad, respectively The 5¢-UTR cDNA sequence of Ubx was cloned into the SacI site of the pCap-FLuc (pLUC-cassette) vector which contains the Firefly luciferase ... mutagenesis on Dm-eIF4B-L plasmids using the QuikChange Site-Directed Mutagenesis Kit (Stratagene) and the primers 5¢-GTGTCCAGGTGAATAACAGCTGGACG AGGAA-3¢ and 5¢-TTCCTCGTCCAGCTGTTATTCA CCTGGACAC-3¢...
- 14
- 366
- 0
Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
Ngày tải lên :
16/03/2014, 06:20
... truncated human 4E-BP1 mutants were generated by PCR amplification using the forward primers (5¢-CGGGATCCCCCCAGGGGTCACTAGCCC TAC-3¢; 5¢-CGGGATCCCCCTGATGGAGTGTCGGA ACTC-3¢; 5¢-CGGGATCCCCGGCGGCACGCTCTTCA ... 5¢-CGGGATCCCCGGCGGCACGCTCTTCA GC-3¢; 5¢-CGGGATCCCCCGCCGCGTAGCCCTCGG-3¢) and reverse primer (5¢-GATGAATTCTAAATGTCCAT CTCAAACTGTG-3¢) 4E-BP1 PCR fragments were cloned into the pGEX-3X vector (BamHI, EcoRI) for bacterial ... (20 03) Inactivation of the tuberous sclerosis complex-1 and -2 gene products occurs by phosphoinositide 3-kinase ⁄ Akt-dependent 21 98 15 16 17 18 19 20 21 22 23 24 25 26 27 and -independent phosphorylation...
- 15
- 337
- 0
Báo cáo khoa học: Autophosphorylation of heme-regulated eukaryotic initiation factor 2a kinase and the role of the modification in catalysis doc
Ngày tải lên :
28/03/2014, 23:20
... 2 1617.7578 1 123 .5056 21 67.9788 1617.7691 1 123 .5063 21 67.9803 89 20 83 1086.8777 1086.8788 2 2171.7408 21 71.7430 21 71.7391 21 71.7391 42 42 615 .27 81 1030.41 92 2 122 8.5416 20 58. 823 8 122 8.5377 20 58. 821 4 ... for Scienti c Research to J.I (21 77130 and 21 117501) and T.S (171010 02) , and Special Education and Research Expenses from the Ministry of Education, Culture, Sports, Science, and Technology of ... 122 8.5377 20 58. 821 4 36 54 10 82. 75 52 324 5 .24 38 324 5 .22 57 72 845.9799 25 34.9179 25 34.8756 24 448. 729 0 448.7307 427 .20 18 505 .25 66 1139.5358 570 .27 53 2 2 895.4434 895.4468 8 52. 3890 1008.4986 1138. 528 5 1138.5360...
- 11
- 498
- 0
Báo cáo khoa học: Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2a (eIF2a) kinase pdf
Ngày tải lên :
31/03/2014, 07:20
... experiment to amplify nucleotides 26 46–3778 of the DPERK cDNA One tenth of the PCR reaction was loaded on a 1% agarose gel Primers 5¢-ATGACCATCCGCCCAGCATACAGGCCCAAG-3¢ and 5¢-TGAGAACGCAGGCGACCGTTGGGGTTGG ... pYX2 12 vector were introduced into yeast strains J80 (MATa gcn2D ura3– 52 leu2–3 leu2–1 12 trp1-D63 sui2D [SUI2-LEU2]) and J 82 (MATa gcn2D ura3– 52 leu2–3 leu2–1 12 trp1-D63 sui2D [SUI2-S51A LEU2]) ... min, cycle, then 94 C 45 s, 50 C 45 s and 68 C 60 s, for 25 or 40 cycles Primers 5¢-CG CGAGGAGTACGACTACGATGAGGAAGAG-3¢ and 5¢-CACTGATGCGGCTCACTGGAGCTGCTGAAG-3¢ were used for every amplification...
- 14
- 296
- 0
Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt
Ngày tải lên :
07/03/2014, 12:20
... the c- jun promoter was generated by PCR using primers: 5¢-CCCAAAACCACTG GCCTGGTTC-3¢ and 5¢-CACAGGCGCTAGATCTGGG CAG-3¢ This fragment was then cloned into a promoter activity assay vector pOPI3CAT ... which hA-elicited activation of the caspase cascade and the JNK pathway were characterized [23 ] The calculated EC50-value for the concentration dependence of hA cytotoxicity was determined to be 10 ... transfection A total of 1.5 lg of either luciferase-reporter gene construct (pCRE-Luc; Stratagene) or vector DNA was transfected into cells using Fugene reagent (Roche) according to the manufacturer’s...
- 13
- 400
- 0
Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
Ngày tải lên :
07/03/2014, 16:20
... Urso B & James DE (20 00) Signalling through the insulin receptor Curr Opin Cell Biol 12, 22 2 22 8 Saltiel AR & Kahn CR (20 01) Insulin signalling and the regulation of glucose and lipid metabolism ... 21 White MF (20 02) IRS proteins and the common path to diabetes Am J Physiol 28 3, E413–E 422 ˚ 22 Stralfors P & Honnor RC (1989) Insulin-induced dephosphorylation of hormone-sensitive lipase Correlation ... & Garofalo R (20 02) Positive and negative regulatory role of insulin receptor substrate and (IRS-1 and IRS -2) serine ⁄ threonine phosphorylation Biochemistry 41, 70 82 7091 Yu C, Chen Y, Cline...
- 11
- 472
- 0
Báo cáo khoa học: Thrombin-mediated impairment of fibroblast growth factor-2 activity doc
Ngày tải lên :
16/03/2014, 01:20
... J (20 05) Induction of hepatocyte growth factor ⁄ scatter factor by fibroblast clustering directly promotes tumor cell invasiveness Cancer Res 65, 9914–9 922 Garcia-Maya M, Anderson AA, Kendal CE, ... inactivates acidic fibroblast growth factor but not basic fibroblast growth factor Biochemistry 27 , 25 72 25 78 51 Lancellotti S, Rutella S, De Filippis V, Pozzi N, Rocca B & De Cristofaro R (20 08) ... radioimmunoassay J Clin Invest 58, 124 9– 125 8 De Cristofaro R, Rocca B, Vitacolonna E, Falco A, Marchesani P, Ciabattoni G, Landolfi R, Patrono C & Davi G (20 03) Lipid and protein oxidation contribute to a prothrombotic...
- 13
- 345
- 0
Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
Ngày tải lên :
22/03/2014, 17:20
... AAATGGTCGTTTGGCTGAAG AGGAACATCTGGCCAGGCTG ACTTGGGATGCTCCATGGTC CTCAACTTTCTGCCCAGAGG CTGGAAGGCAGAAGTGAAGG CACTCTGGACAGTGCAGGAAG CGACACCTCCAGGAAGCTCTG AAAGGATCCACTGGCGTCTTCACCACC GAATTCGTCATGGATGACCTTGGCCAG Forward ... Oct -2 Relative Oct -2 mRNA (Oct -2/ GAPDH) β-actin D Oct -2 Oct -2 C Oct -2 Rapa 4 p-p70S6K β-actin β-actin p70S6K LPS LPS Rapa LPS Vector DN-mTOR Control Rapa RR-mTOR Vector DNmTOR Fig Rapamycin reduces ... Reverse TGGCTGGAAGAGAGGAAGAG CTGGGGCAACTCAGGCTTA AACTGGGGACTCTCCCTTTG CTACTCCGTGAAGTGAACAA Primers that can be used to amplify both human and mouse Oct -2, iNOS and GAPDH cDNA FEBS Journal 27 8 (20 11)...
- 12
- 376
- 0
Báo cáo khoa học: "Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis" ppt
Ngày tải lên :
07/08/2014, 18:21
... deddebme erew evren citaics eht fo seceiP sisylana nietorp rof Co07− ta nezorf dna devomer erew sevren citaics ehT )tniop emit hcae = n( yad gnihctam hcae ta star lortnoc dezinummi-AFC morf deniatbo ... sllec yrotammalfni mµ 03 = srab elacs eht ,C- A nI sevren citaics eht ni )neerg( sllec nnawhcS evitisop 001S ni dezilacol-oc ton erew )der( noitcaer-LENUT emoS )C( )sworra( wolley gniwohs sroloc ... nnawhcS emos taht ytilibissop eht edulcxe ton od ew ,NAE ni sllec nnawhcS no noitcaer LENUT lacipyt a dnuof ton evah ew hguohtlA ]4[ NAE gnirud sisotpopa ogrednu ot dnuof erew sllec nnawhcS ,yduts...
- 5
- 206
- 0
Báo cáo y học: "Action of fibroblast growth factor-2 on the intervertebral disc" docx
Ngày tải lên :
09/08/2014, 10:23
... GGC TGG GTG TCG 687 60 EMBL: NM_015850 .2 h-FGF2 GAG AAG AGC GAC CCT CAC A TAG CTT TCT GCC CAG GTC C 27 8 58 EMBL: NM_0 020 06.3 h-ADAMTS5 CCC TCT TCC CTG TGC AGT AG CTA CGA TGC CAC CCA GCA G 363 58 ... Bov-MMP-13 ACC CTT CCT TAT CCC TTG ATG CCA AAA CAG CTC TGC TTC AAC CTG CTG 110 55 EMBL: NM_174389 .2 Bov-ADAMTS ACT GGG CTA CTA TTA CGT GGA AAA CAC ACA CCA TGC ACT TGTCGA ACT 155 60 EMBL: BC148059.1 ... Bov-FGFR4 GCT GAT TGG CCG ACA CAA GAA CAT AGC ACA CTC CAC GAT CAC GTA CAA 85 55 EMBL: XM_6 021 66.3 Bov-noggin TCT GTA ACT TCC TCC GCA GCT TCT AGC GAG ATC AAA GCG CTG GAG TT 88 55 EMBL: XM_5 825 73.3...
- 12
- 584
- 0
Báo cáo y học: "Role of epidermal growth factor receptor activation in regulating mucin synthesis" pdf
Ngày tải lên :
12/08/2014, 18:20
... with mucus accumulation in the 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 airways of patients who died of severe asthma attack Chest 19 92, 1101:916– 921 Burgel P-R, Escudier E, Coste ... investigators to occur in asthma, and this damage could induce mucus hypersecretion Second, because chronic intubation leads to mucus hypersecretion in horses [14], a similar effect is likely to occur ... EGFR tyrosine phosphorylation and a subsequent downstream cascade, which causes mucin gene and protein expression goblet-cell metaplasia First, NCI-H2 92 cells (an epidermoid carcinoma cell line that...
- 5
- 375
- 0
báo cáo khoa học: " Inadvertent malposition of a permanent pacemaker ventricular lead into the left ventricle which was initially missed and diagnosed two years later: a case report" pptx
Ngày tải lên :
11/08/2014, 00:22
... diaphragmatic pacing and loss of capture [3, 12] The exact risk of thromboembolism secondary to the presence of a pacing lead in the LV is unknown, but the incidence may reach up to 37% as suggested ... concomitant cardiac surgery [14] In our patient, it was decided to leave the lead in place and to continue lifelong anticoagulation Conclusions Inadvertent insertion of pacing and internal cardioverter ... Millar K: Malplacement of pacemaker catheters in the coronary sinus: recognition and clinical significance J Thorac Cardiovasc Surg 1969, 57:511-518 Mazzetti H, Dussaut A, Tentori C, Dussaut E,...
- 5
- 235
- 0
Báo cáo y học: "Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells" pot
Ngày tải lên :
12/08/2014, 01:21
... post-infection (hpi) to detect RNA synthesis through amplification of a gene fragment by RT-PCR The primer pair (D2EL: TAACACCACAGAGTTCCATC and D2ER: TAAACTTTCCTGTGCACATA) was used to detect newly synthesized ... Oligo dT and the primer derived from selected clones of eIF5A (eIF5AL: 5’-TATTTGCCCATCCACTCACA) The products were then cloned into the pGEM-T vector to subsequently sequence the 3’-end of the gene ... to remove the genomic DNA, from which 5’-end cDNA was generated with gene- specific primer (GSP)-1, 5’-CGATGCCAACCAGATGTACC-3’, and Superscript II™ RT (Invitrogen) dCTP was added to the tail of...
- 9
- 240
- 0
Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
Ngày tải lên :
22/02/2014, 07:20
... from K5 62 cells and the oligonucleotides 5¢-CCCCACTA GTAATTCCAGCGAGAGGCAGA-3¢ and 5¢-AATACC ATGGTCGCGGGAGGCTGCTG-3¢, and was ligated to the StuI–NcoI 7.5-kb fragment of pLL to generate pLML pcK3L ... encoding proteins involved in cell growth and differentiation such as growth factors, receptors, transcription factors, protooncogenes, and cytokines [10] Using the 1 022 nucleotide long 5¢UTR of platelet-derived ... by PCR of the eIF2a cDNA from p51A and p51D [20 ], respectively, using the oligonucleotides 5¢-CTGGATATCATGCCGGGTCT AAGTTG-3¢ and 5¢-CTGCTCGAGTTAATCTTCAGCT TTGGC-3¢, followed by ligation into the...
- 10
- 409
- 0
Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
Ngày tải lên :
19/02/2014, 18:20
... Ras-GRF1 gene- speci c primers: ON357, 5¢-TGAAACATCACCAACTAAATC TCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGG AAAAGAGT-3¢; ON359, 5¢-GCCGCTGGAGAAACAG CAT-3¢; ON360, 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, ... Mattingly RR & Macara IG (1996) Phosphorylationdependent activation of the Ras-GRF ⁄ CDC25Mm exchange factor by muscarinic receptors and G -protein bc subunits Nature 3 82, 26 8 27 2 Farnsworth CL, Freshney ... Journal 27 2 (20 05) 23 04 23 16 ª 20 05 FEBS Ras-GRF1 and Ras-dependent ERK activation in HEK293 15 16 17 18 19 20 21 22 23 24 25 signaling in the cerebral cortex J Biol Chem 27 8, 1 327 8–1 328 5 Shou C, ...
- 13
- 730
- 0
Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf
Ngày tải lên :
06/03/2014, 00:21
... GTCGGATC CGCGGATCAGGTGGGGATGTATTA; rnc5¢I comp, GGCAGTGGATGATGGGGTTCATGCGATACC; rnc3¢O SalI, TGCGTCGACATTTGCCGCAATAGTGTCAACA; and rnc3¢I comp, TGAACCCCATCATCCACTGCCAG GTCAGCG The deletion was constructed ... era_F_NcoI, CGACCATGGCGAAC AGGCGTTGAAAAAAC; and era_R_SalI, CGAGTCGA CAGCCTTCCATCGGAGTTACT The resulting vector 1754 Selection of second-site rRNA suppressors of cold-sensitive IF1 A direct selection ... function of Escherichia coli pre -23 S rRNA Proc Natl Acad Sci USA 81, 185–188 Croitoru V, Bucheli-Witschel M, Hagg P, Abdulkarim F & Isaksson LA (20 04) Generation and characterization of functional...
- 12
- 439
- 0
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
Ngày tải lên :
06/03/2014, 11:20
... chain factors, eukaryotic class polypeptide chain release factor (eRF1) and eukaryotic class polypeptide chain release factor (eRF3) The major functions of eRF1 include recognition of each of the ... Kisselev LL (20 06) Termination of translation in eukaryotes is mediated NMR structure and function of the eRF1 C- domain 18 19 20 21 22 23 24 25 26 27 28 29 by the quaternary eRF1*eRF3*GTP*Mg2+ complex ... conformer Structure visualization and analysis were carried out using the insightii software package (Accelrys Software Inc.) and pymol (DeLano Scienti c LLC) FEBS Journal 27 7 (20 10) 26 11 26 27 ª 20 10...
- 17
- 490
- 0
Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot
Ngày tải lên :
07/03/2014, 01:20
... anti-hsMOK2 serum Cells were observed with a Leica DMR microscope and an Apochromat 63 · 1. 32 oil immersion objective (C) HeLa cells were co-transfected with expression vector for lamin C Q294P and ... of a gene with properties of the transcription factor E2F Mol Cell Biol 12, 5 620 –5631 24 Chellappan SP, Hiebert S, Mudryj M, Horowitz JM & Nevins JR (1991) The E2F transcription factor is a cellular ... introduced into hsMOK2 constructs using the QuickChange XL site-directed mutagenesis kit (Stratagene, Amsterdam, The Netherlands) The prokaryotic expression vector pET29, containing Aurora A cDNA,...
- 11
- 378
- 0