stress responses of lactococcus lactis
Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... cultivation Lactococcus lactis ssp lactis IL1403 is a derivative of the strain IL594 that was isolated from a cheese starter culture [47] For the isolation of genomic DNA and the creation of stock ... insight into the chitinolytic potential of L lactis, the present results provide only the third example of the role of family 33 CBPs in the degradation of recalcitrant polysaccharides Furthermore, ... example of a family 33 CBP that promotes the degradation of a-chitin, the most abundant chitin form in nature Results and Discussion Preliminary assessment of the production of chitinases by L lactis...
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Tài liệu Báo cáo khoa học: AcmA of Lactococcus lactis is an N-acetylglucosaminidase with an optimal number of LysM domains for proper functioning ppt
... sequence of the lactic acid bacterium Lactococcus lactis ssp lactis IL1403 Genome Res 11, 731–753 12 Buist G (1997) AcmA of Lactococcus Lactis, A Cell Binding Major Autolysin PhD thesis, University of ... by RP-HPLC Acknowledgements Isolation of AcmA from the supernatant of L lactis cultures The cells of 100 mL of L lactis MG1363acmAD1 were boiled in 20 mL of 10% SDS for 15 min, washed six times ... of the AcmA derivatives A1, A2, A3 and A4 to L lactis cells Stationary phase cells from mL of L lactis MG1363acmAD1 culture were mixed with the supernatant of stationary phase cultures of L lactis...
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Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx
... concentration of the exofacial thiol groups during reduction by Lactococcus lactis TIL 46 (A) Time course evolution of growth ( ) and time course evolution of concentration of exofacial thiol ... the column Evolution of exofacial thiol groups during medium reduction by L lactis The concentration of accessible exofacial thiol groups was monitored during the growth of L lactis (Fig 5A) Before ... groups in the reducing activity of L lactis D Michelon et al Table Effect of ltration on the Eh7 value of MRS media reduced by three different strains of Lactococcus lactis Filtration was carried...
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Use of lactococcus lactis as a mucosal vaccine delivery vehicle
... nasal administration of L lactis strains Detection of anti-L lactis IgG antibodies in the serum of C57BL/6 mice after nasal administration of L lactis strains Detection of anti-L lactis IgG antibodies ... serum of BALB/c mice after oral administration of L lactis strains Detection of anti-L lactis IgG antibodies in the serum of C57BL/6 mice after oral administration of L lactis strains Detection of ... serum of BALB/c mice after nasal administration of L lactis strains Detection of anti-EDIII IgG antibodies in the serum of C57BL/6 mice after nasal administration of L lactis strains Detection of...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
... (2004) Effect of pyruvate kinase overproduction on glucose metabolism of Lactococcus lactis Microbiology 150, 1103–1111 Thomas S & Fell DA (1998) A control analysis exploration of the role of ATP utilisation ... glycolytic flux in Lactococcus lactis MG1363 J Bacteriol 185, 1564–1571 Llanos RM, Harris CJ, Hillier AJ & Davidson BE (1993) Identification of a novel operon in Lactococcus lactis encoding three ... phosphofructokinase, pyruvate kinase, and lactate dehydrogenase J Bacteriol 175, 2541–2551 Andersen HW, Solem C, Hammer K & Jensen PR (2001) Twofold reduction of phosphofructokinase activity in Lactococcus...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... activation of the glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of UTP and ATP-cS (C) GTP activation of CTP synthesis at 0.1 mM each of UTP and ATP L lactis ... time are derived from the heat of dilution of glutamine into the reaction cell (B) The heat generated by hydrolysis of 0.15 lM of glutamine used to determine DH of the reaction ể FEBS 2002 4774 ... a poor inhibitor of L lactis CTP synthase compared to ATP-cS On the basis of these results, ATP-cS was chosen as a binding analog of ATP CTP synthesis requires the presence of both the nucleotide...
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RESPONSES OF ORGANISMS TO WATER STRESS docx
... biochemical responses of plants to drought stress African Journal of Agricul tural Research 2011; 6(9):2026-32 [13] Sakamoto A, Murata N The role of glycinebetaine in the protection of plants from stress: ... cited 18 Responses of Organisms to Water Stress been made, we still not fully understand the underlying survival mechanisms in plants due the complex interaction of different forms of stress in ... forms of abiotic stress that induce an increase in the generation of ROS depends on the development of efficient ROS-scavenging mechanisms 4.2 Chemistry of ROS Much of the behaviour of molecular...
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Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc
... resuspended in mL of 0.9% (w/v) NaCl Fifty milliliters of SA medium with glucose and erythromycin was inoculated to D436 of 0.025 and the growth monitored by measuring D436 At D436 of 0.8, 35 mL of the ... enzyme for growth of L lactis (Deoxy)ribonucleotide pool sizes in mutants with different levels of pyrG expression Figure 4A shows the variation of the CTP concentration as a function of pyrG expression ... stippled lines Fig Eects of changing pyrG expression on the concentration of dCTP The concentration of dCTP is shown as a function of relative b-galactosidase activity in percent of the wild-type level...
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Báo cáo khoa học: Molecular responses of Campylobacter jejuni to cadmium stress ppt
... in the response of C jejuni to cadmium stress that are unique to it as well as others that are common with the responses of other bacteria The modulation of expression of enzymes of fatty acid ... effects of oxidative stress This response is found in E coli, in which cadmium upregulated proteins of heat shock and oxidative stress regulons [43] Similarly, exposure of anterior gills of the ... subjected to metal stress [26] Oxidative phosphorylation generates high-energy ATP, and upregulation of the expression of this synthase may serve to offset the downregulation of expression of TCA cycle...
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Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... any of the combinations of inhibitors A similar conclusion can be drawn for the combination of ADP + NAD for ADH, whereas there was a slight increase in inhibition of ADH by the combination of ... GAPDH of L lactis ATCC 19435 investigated might be a result of various isozymes of each of these dehydrogenases because of the use of cell extracts However, unpublished transcriptomics results ... the ux of ATP production through strong nonlinear inhibition of ADH: by obtaining two instead of three ATPs per sugar unit in excess concentrations of ATP and ADP [1] Inhibition kinetics of dehydrogenases...
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Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase ... FEBS S I Yang and T Tanaka Recombinant Lactococcus lactis prolidase Table The relative activities of recombinant Lactococcus lactis prolidase in the presence of zinc or manganese for various peptide ... structure of P furiosus prolidase and the sequence-based model of Lc lactis prolidase (Fig 5) indicated that the S1 sites are composed mainly of hydrophobic residues (Phe190, Leu193 and Ile308 of Lc lactis...
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Báo cáo khoa học: Natural-abundance isotope ratio mass spectrometry as a means of evaluating carbon redistribution during glucose–citrate cofermentation by Lactococcus lactis potx
... redistribution in L lactis cofermentation (Eur J Biochem 271) 4397 Table Values of d13C (&) determined for products of fermentation of Lactococcus lactis with differing initial amounts of glucose and ... studies of citrate and glucose cometabolism by Lactococcus lactis Appl Environ Microbiol 60, 1739–1748 Verhue, W & Tjan, F (1991) Study of the citrate metabolism of Lactococcus lactis subsp lactis ... Physiology of pyruvate metabolism in Lactococcus lactis Antonie Leeuwenhoek 70, 253–267 Rondags, E., Halliday, E & Marc, I (1998) Diacetyl production mechanism by a strain of Lactococcus lactis spp lactis...
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Báo cáo y học: "Heterogeneity of psychophysiological stress responses in fibromyalgia syndrome patients" pps
... increase of BP, HR, and SCLs in the stress compared to the BL phase and the reduction in the REL phases indicates that stress and relaxation were induced, confirming the ecological validity of the stressors ... medication affects autonomic nervous system activity However, a comparison of the physiological stress responses of the subgroups of patients with (n = 20) and without (n = 60) antidepressant medication ... asked to rate the intensity of their pain and perceived stress on an 11 point scale with the endpoints 'no pain' to 'very intense pain', and 'not at all stressful' to 'very stressful' Psychophysiological...
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báo cáo khoa học: " Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses" ppt
... the exception of the C3H family, members of these families are reportedly capable of abiotic stress responses [51-53] As CBF TFs are reported to participate in many abiotic stress responses, Mtr.38878.1.S1_at ... groups: (a) up-regulated profiles (b) down-regulated profiles Numbers of probe sets assigned to each profile are represented below P-values are shown in left bottom of each profiles, only statistically ... resistance to reactive oxygen species (ROS) stress [70], which often occurs after ion stress caused by salt stress Isoflavonoids are required for a wide range of essential physiological processes and...
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Báo cáo y học: "Experimental stress in inflammatory rheumatic diseases: a review of psychophysiological stress responses" doc
... neuroendocrine responses, or immune responses (see Tables 2, and 4) Self-reported distress Stress- induced self-reported distress was assessed in only two of 16 studies [41,45] Subjective distress levels ... to stress Psychosocial stress might prove to be the best tool to evaluate these specific immune responses to stress Psychosocial stressors A psychosocial stressor was used in three studies of ... suppression test) and psychological stressors (cognitive tests, public speaking) [2] Many studies have investigated the effects of these types of stress on components of the stress response system, such...
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Báo cáo sinh học: "Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120" ppt
... analysis of gp120 Expression analysis of gp120 In vitro expression of gp120 protein from L lactis (pLL120) and E coli (pEC120) plasmids with and without CpG motifs RIPA of culture supernatants of 35S-cys/met-pulsed ... the importance of animal studies Because of the complex nature of the adjuvant effect of plasmid DNA, we measured the resulting effect of in vivo DNA vaccination using large amounts of DNA as routinely ... production of plasmid DNA based on Lactococcus lactis with improved safety properties concerning the microbial production host and the genetic content of the plasmid [10] As host L lactis is attractive...
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Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change pptx
... Adaptations and Stress Tolerance of Plants in the Era of Climate Change Parvaiz Ahmad ● M.N.V Prasad Editors Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change ... crops Ion imbalance and osmotic stress is the primary effect of abiotic stress Prolonged exposure to primary stress causes secondary stress through the generation of reactive oxygen species (ROS) ... regulate various responses to stress Plants respond the abiotic stress by up- and downregulation of genes responsible for the synthesis of osmolytes, osmoprotectants, and antioxidants Stress- responsive...
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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... maximum at a pH of about 5.5 The pH dependence of this activation fitted well with a pKa of 6.3 ± 0.1 Lactacte dehydrogenases of Lactococcus lactis Fig Effect of pH on the activity of LDHB (d) and ... impact on the physiology of L lactis of Lactacte dehydrogenases of Lactococcus lactis replacing LDH by a homofunctional protein with different kinetic properties Comparison of maximal LDH and LDHB ... Lactacte dehydrogenases of Lactococcus lactis 16 17 18 19 20 21 22 23 24 25 26 27 28 of different NADH oxidase levels on glucose metabolism by Lactococcus lactis: kinetics of intracellular metabolite...
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