stem progenitor cells modified by zinc finger nucleases targeted to ccr5 controls hiv 1 in vivo
Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
Ngày tải lên :
10/08/2014, 21:23
... expanded cells, × 10 15 WT-like B6.SJL Sall4B expanded cells, × 10 6 11 WT-like B6.SJL GFP-infected cells, × 10 6 15 WT-like 11 WT-like B6.SJL Number of injected cells GFP-infected cells, × 10 Month ... not convert cells to cytokine independence In the combination of cytokines, the Sall4A or Sall4B transduced cells exhibited a ~2 fold increase in the rate of proliferation relative to the GFP ... convert the 32D cells to cytokine independence In agreement with previous reports [ 21, 22], 32D cells proliferate as undifferentiated blasts when maintained in IL-3, but differentiate into mature granulocytes...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx
Ngày tải lên :
18/06/2014, 19:20
... anti-apoptoric protein human surviving is a direct inhibitor of caspase-3 and Biochemistry 20 01, 40 :11 17 -11 23 Zhu W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in ... improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6 stem cell 2009, 5:54-63 doi :10 .11 86 /14 79-5876-9 -10 5 Cite this article ... http://www.translational-medicine.com/content/9 /1/ 105 16 17 18 19 20 21 22 23 Page 10 of 10 Apoptosis and Promotes Endogenous Cell Proliferation After Stroke in Female Rat J Neuro Res 2003, 73:778-786 Shin S, Sung...
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o cáo hóa học:" Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" docx
Ngày tải lên :
20/06/2014, 04:20
... anti-apoptoric protein human surviving is a direct inhibitor of caspase-3 and Biochemistry 20 01, 40 :11 17 -11 23 Zhu W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in ... improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6 stem cell 2009, 5:54-63 doi :10 .11 86 /14 79-5876-9 -10 5 Cite this article ... http://www.translational-medicine.com/content/9 /1/ 105 16 17 18 19 20 21 22 23 Page 10 of 10 Apoptosis and Promotes Endogenous Cell Proliferation After Stroke in Female Rat J Neuro Res 2003, 73:778-786 Shin S, Sung...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
Ngày tải lên :
30/03/2014, 04:20
... H HIF -1 β-Actin D 1. 8 * HIF -1 / actin 1. 6 1. 4 1. 2 * * * * 24 h 48 h * 0.8 0.6 0.4 0.2 1h Expression of HIF-1a in NPCs under hypoxia (10 %) H 18 SRNA Bromodeoxyuridine incorporation assay Incorporation ... significant increase in viral toxicity The expression of HIF-1a protein showed an increase after infection with Ad–HIF-1a (Fig 3A) The total number of cells in NPCs infected with Ad–HIF-1a increased in ... HIF-1alpha and an autocrine angiotensin system FASEB J 19 , 857– 859 40 Salceda S & Caro J (19 97) Hypoxia-inducible factor1a (HIF-1a) protein is rapidly degraded by the ubiquitin– proteasome system...
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Báo cáo khoa học: " Inactivation of respiratory syncytial virus by zinc finger reactive compounds" ppsx
Ngày tải lên :
12/08/2014, 04:21
... RSV inactivation by AT-2 in accompanied by modification of the M2 -1 protein The fact that a compound acting by covalently modifying zinc finger motif containing proteins of retroviruses can inactivate ... Moreover, the intact zinc finger motif of the M2 -1 protein is required for maintaining functional integrity of the protein [20] The zinc finger motifs of RSV M2 -1 protein and NC proteins of retroviruses ... more effective in inactivating RSV faster Zinc finger reactive compounds inhibiting HIV- 1 infectivity have been identified by the National Cancer Institute’s drug screening program [14 ] A similar...
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Báo cáo y học: " Identity of zinc finger nucleases with specificity to herpes simplex virus type II genomic DNA: novel HSV-2 vaccine/therapy precursors" pps
Ngày tải lên :
13/08/2014, 16:20
... of HIV replication in- vivo using small artificial molecules modified to harness target DNA, a binding mechanism inherent in zinc finger (ZF) domains as a strategy to repress HIV transcription, ... individual zinc finger usually recognizes three nucleotide bases, but many zinc fingers can be combined to generate an array capable, in the case of our listed ZFAs of three fingers, of recognizing nine ... Barbas CF: Zinc finger tools: custom DNA-binding domains for transcription factors and nucleases Nucl Acids Res 2006, 34(SI):W 516 -W523 36 Dreier B, Segal DJ, Barbas CF: Insights into the molecular...
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Báo cáo y học: " Proviral HIV-genome-wide and pol-gene specific Zinc Finger Nucleases: Usability for targeted HIV gene therapy Misaki Wayengera" ppt
Ngày tải lên :
13/08/2014, 16:20
... Assembly of HIV- pol gene /HIV- 1- proviral -dsDNA binding zinc finger arrays and construct of HIV- pol gene /HIV- 1- proviral-dsDNA cleaving zinc finger nucleases First, using the Zinc Finger Consortium’s ... HIV- 1 infection within NSG mice transplanted with human hematopoietic stem/ progenitor cells modified by zinc- finger nucleases targeting CCR5 On the other hand, with the intent of disrupting incoming ... PD, Holmes MC, Cannon PM: Human hematopoietic stem/ progenitor cells modified by zinc- finger nucleases targeted to CCR5 control HIV- 1 in vivo Nat Biotechnol 2 010 , 28(8):839-847 29 Grosse S, Huot...
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Báo cáo y học: "Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites" pdf
Ngày tải lên :
13/08/2014, 09:21
... transcriptional termination are indicated to the right Read through to the 5' end of the HIV- 1 RNA is denoted by +1 A sequence latter obtained by dideoxysequencing of the HIV- 1 RNA is included in lanes ... Mock AUG Relative CA-p24 (x10-5) B Conc (in nM) 0 ,16 0,8 20 LNAPBSD C Relative CA-p24(x10-6) 12 10 Conc (in nM) 20 10 0 20 10 0 20 10 0 20 10 0 20 10 0 20 10 0 20 10 0 20 10 0 PBSD DIS LNA antisense ... response element 10 PBS: Primer binding site 11 DIS: Dimer initiation site 12 SD: Major splice donor 13 PSI: Packaging signal 14 PBSD: Primer binding site downstream Competing interests 15 The author(s)...
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Báo cáo y học: "A cyclic-RGD-BioShuttle functionalized with TMZ by DARinv “Click Chemistry” targeted to αvβ3 integrin for therapy"
Ngày tải lên :
25/10/2012, 11:40
... Mass: 16 33.64; Mol Wt.: 16 34.84 m/e: 16 33.64 (10 0.0%), 16 34.64 (97.8%), 16 35.65 (39.3%), 16 36.65 (14 ,2%), 16 36.64 (11 .9%), 16 35.64 (10 .8%), 16 35.63 (9.4%), 16 37.65 (4,7%), 16 37.64 (4,2%), 16 38.64 ... http://www.medsci.org Int J Med Sci 2 010 , References 10 11 12 13 14 15 16 17 18 19 20 NIH Consensus Statement Breast cancer screening for women ages 40-49 NIH Consens Statement 19 97; 15 : 1- 35 Harms JF, Welch ... αvβ5 integrin receptor into the perinuclear region of HeLa cells is documented, which lack the functional αvβ3 receptor. [10 ] Interestingely HeLa cells, which express the αvβ3 integrin receptor...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
Ngày tải lên :
20/02/2014, 11:20
... 2.30 1. 50 4.87 1. 75 4.27 2 .12 1. 70 1. 24 2.69 2.00 4.36 5.02 1. 59 2.45 · · · · · · · · · · · · · · · a )4 10 10 )4 10 )4 10 )4 10 )3 10 )5 10 )6 10 )6 10 )5 10 )4 10 )4 10 )5 10 )6 10 )5 10 )5 c d Type of inhibition ... N-terminal MWP motif were determined to be in the range 10 )6 M to 10 )5 M indicating that compounds with tryptophan in position were the most potent inhibitors we examined Comparing Tat (1 9) and ... Asp2 inhibited DP IV with clearly lower Ki values (10 -5 )10 -6 M) and according to the linear mixed-type inhibition mode characterized by inhibitor binding only to the noncompetitive binding site...
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Báo cáo y học: " Proteasome-independent degradation of HIV-1 in naturally non-permissive human placental trophoblast cells" pptx
Ngày tải lên :
12/08/2014, 23:21
... 6:46 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Campbell EM, Perez O, Anderson JL, Hope TJ: Visualization of a proteasome-independent intermediate during restriction of HIV- 1 by rhesus ... trophoblast cells than infectious VSV-G HIV- 1 pseudotypes Although HIV- 1 particles are able to gain intracellular access to trophoblast cells, these cells are unable to sustain HIV- 1 replication; ... described in this report is to gain insight into the mechanism of restriction of cell-free HIV1 infection in the BeWo trophoblast cell line by investigating viral entry in both wild-type and CD4+ cells...
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Báo cáo y học: "WFDC1 expression identifies memory CD4 Tlymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions" pptx
Ngày tải lên :
13/08/2014, 01:20
... J-ps20high * 10 0 .1 0 .1 0. 01 1 :1 1:0.2 uninfected target:infected cell ratio (D) 0. 01 1 :1 1:0.2 uninfected target:infected cell ratio (E) 10 0 10 0 10 * ** 10 1 0 .1 0 .1 0. 01 + J-ps20inter J-ps20inter ... interval:0.003234 to 0.5503 P value (two-tailed): 0.0 418 10 -1 (A) 10 -2 (B) 12 5 10 -3 75 10 -4 -5 * * 50 -6 10 siNS siICAM -1 siPs20 10 0 25 10 10 -7 10 -6 10 -5 10 -4 10 -3 10 -2 10 -1 10 ** ps20 ICAM -1 GAPDH ... ICAM -1 and integrins (e.g like LFA -1 and a4b7 [17 ,18 ,24-27]), can be exploited by viruses like HIV- 1 to promote spreading infection Specifically, budding cell-free HIV- 1 particles that incorporate...
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Báo cáo y học: " Centrosomal pre-integration latency of HIV-1 in quiescent cells" docx
Ngày tải lên :
13/08/2014, 05:22
... http://www.retrovirology.com/content/4 /1/ 63 Sub-cellular localization of incoming HIV- 1 in quiescent CD4+ T cells Figure Sub-cellular localization of incoming HIV- 1 in quiescent CD4+ T cells A Incoming HIV- 1 CA localizes ... 2007, 4:63 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 within resting CD4+ T cells while short ones are degraded AIDS Res Hum Retroviruses 2004, 20(3):285-295 Spina CA, Guatelli ... http://www.retrovirology.com/content/4 /1/ 63 Figure Incoming HIV- 1 persistently reside at the centrosome of resting cells Incoming HIV- 1 persistently reside at the centrosome of resting cells A Cycling but not resting MRC5 cells...
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Báo cáo y học: " Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS" ppt
Ngày tải lên :
13/08/2014, 05:22
... of amino acid variability in NSI-like and SI-like V3 sequences and a linked change in the CD4-binding domain of the HIV- 1 Env protein Virology 19 97, 239 (1) :10 8 -11 8 Nabatov AA, Pollakis G, Linnemann ... V3-containing HIV- 1 gp120 core Science 2005, 310 (5750) :10 25 -10 28 Sobolev V, Sorokine A, Prilusky J, Abola EE, Edelman M: Automated analysis of interatomic contacts in proteins Bioinformatics 19 99, 15 (4):327-332 ... 20 (1) :11 1 -12 6 Connor RI, Sheridan KE, Ceradini D, Choe S, Landau NR: Change in coreceptor use coreceptor use correlates with disease progression in HIV- 1 infected individuals J Exp Med 19 97, 18 5(4):6 21- 628...
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Báo cáo y học: "Inactivation of HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS)" pot
Ngày tải lên :
13/08/2014, 09:21
... treating infected Sup-T1 cells (CD4+ human T cells) with SDS ( 1% ) for 10 at 37°C prior to overlaying on P4R5 cells In brief, × 10 6 Sup-T1 cells were infected with a 1: 10,000 dilution of stock HIV- 1 ... time-course of inactivation of cell-associated HIV- 1 Sup-T1 cells in media infected with HIV- 1 IIIB were treated for up to 15 with 0. 01% SDS and assayed for infectivity using P4-R5 indicator cells Samples ... to observe inactivation of HIV- 1 (Figure 1B) Infectivity of cell-associated HIV- 1 (i.e., HIV- 1- infected Sup-T1 cells) was abolished with treatment with 0 .1% SDS This inactivation was due to induced...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
Ngày tải lên :
18/06/2014, 16:20
... during hematopoietic stem cell mobilization induced by G-CSF or cyclophosphamide J Clin Inves 2003, 11 1 :18 7 -19 6 69 Cavaillon JMH-CN: Signals involved in interleukin synthesis and release by lipopolysacchride-stimulated ... started to drop on day 14 of administration This may be a physiological response towards maintaining a constant level of circulating progenitor cells Indeed it may be possible that Stem- Kine could ... acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells J Am Coll Cardiol 2004, 43:2 314 -2 318 Rich IN: In vitro hematotoxicity testing in drug...
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Báo cáo sinh học: "MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells" pptx
Ngày tải lên :
18/06/2014, 19:20
... TE10/Het-1A(fold) OE 21/ Het-1A (fold) 300 25 OE- 21/ Het-1A TE -10 /Het-1A 250 20 200 15 15 0 10 10 0 50 microRNA 203 429 205 200c 14 1 200b A Het-1A/OE 21 (fold) 90 Het-1A/OE- 21 OE- 21/ Het-1A 80 Het-1A/TE -10 ... Het-1A/TE -10 TE -10 /Het-1A 70 60 50 40 30 20 10 microRNA 15 3 10 0 12 5b 10 a 99a 376a Het-1A/TE10 (fold) 14 12 10 379 6 51 146b B Figure The comparison of miRNA profile in ESCC cell lines (OE 21 or TE10) ... 50nM 10 nM miR-205 precursor scramble 10 nM 50nM anti-miR-205 inhibitor B % apoptotic cells % 0 .14 apoptotic cells 0 .12 12 0 .1 10 0.08 0.06 72hrs Apoptosis Het1A Ratio 12 10 Total cell count Het1A...
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báo cáo khoa học: "Compound Kushen Injection suppresses human breast cancer stem-like cells by down-regulating the canonical Wnt/b-catenin pathway" potx
Ngày tải lên :
10/08/2014, 10:21
... to injection of SP cells (1 × 10 4 cells, × 10 3 cells) compared with non-SP injection (1 × 10 4 cells, × 10 3 cells) (C) A representative tumor in a mouse specimen at the SP injection (1 × 10 3 cells) ... http://www.jeccr.com/content/30 /1/ 103 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Bao S, Wu Q, Mclendon RE, Hao Y, Shi Q, Hjelmeland AB, Dewhirst MW, Bigner DD, Rich JN: Glioma stem cells promote radioresistance by preferential ... 2 011 Accepted: 28 October 2 011 Published: 28 October 2 011 References Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 20 01, 414 :10 5 -11 1 Gottesman MM:...
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Báo cáo y học: "Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion" potx
Ngày tải lên :
12/08/2014, 14:22
... NM_002207 CHRNA3 2 .10 up 12 .73 down BC00 611 4 IL11RA 10 .76 down 6.66 up NM_004 512 integrin, alpha 211 772_x_at cholinergic receptor, nicotinic, alpha 204773_at interleukin 11 receptor, alpha Membrane ... up AK024252 2 018 49_at BCL2/adenovirus E1B 19 kDa interacting protein BNIP3 69.04 down 11 .78 up NM_004052 CD59 18 .76 down 2.72 up NM_000 611 NBN MAPK 11 12.38 down 17 .27 up 4.28 up 10 .62 down AF049895 ... kinase 11 Response to wounding and wound healing 209277_at Tissue factor pathway inhibitor TFPI2 9.85 down 2.62 up LMAN1 11 .53 up 9.84 down U09 716 EREG CTGF 5 .13 down 595.44 down 3. 61 down 14 .43...
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