stable isotope labeling with amino acids in cell culture based proteomic analysis of ethanol induced protein expression profiles in microglia
Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
Ngày tải lên :
17/03/2014, 03:20
... of the plasmin activity was inhibited) Computer model of the C-terminal 40 amino acids in antiplasmin A computer model of the C-terminal 40 amino acids in antiplasmin [11] was constructed by CS ... lysine-binding-site-mediated interaction between plasmin and antiplasmin Molecular modelling of the C-terminal portion of antiplasmin The amino- acid sequence of the C-terminal 40 residues in antiplasmin ... plasmin molecules [7,8] In addition, it is known that the lysine-binding sites in plasminogen are important for binding to other proteins, such as receptor proteins at the cellular surface, in...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
Ngày tải lên :
22/03/2014, 21:21
... provided insights into important enzyme–substrate interactions For example, molecular modeling of HC interacting with angiotensin (Ang) I has led to conclusions regarding the S2¢ binding site of HC ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates ... specificity of the human mast cell chymase Discussion Acidic amino acids in P2′ (%) 70 Glu Asp 60 50 40 30 20 10 HC wt R143Q K192M R143Q + K192M Fig Distribution of acidic amino acids in position P2¢ of...
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Báo cáo y học: " Albumin dialysis improves hepatic encephalopathy and decreases circulating phenolic aromatic amino acids in patients with alcoholic hepatitis and severe liver failure" doc
Ngày tải lên :
13/08/2014, 11:23
... The lack of effect of albumin dialysis in significantly modifying the amino acid profile in primary biliary cirrhosis patients with bilirubin, prothrombin index and albumin levels within normal ... Albumin (g/L) Haemoglobin (g/L) Leucocytes Platelets (cell/ ×109) (cell/ ×109) Total amino acids (μM/L) AA = amino acids; ALT = alanine aminotransferase AP = alkaline phosphatase; AST = aspartate aminotransferase; ... effect was associated with significant changes in the levels of circulating amino acids, and particularly with changes in phenolic aromatic amino acids, which decreased in patients with alcoholic hepatitis...
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Microbial Production of Amino Acids in Japan
Ngày tải lên :
26/10/2013, 02:20
... contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in the ... amounts of l-lysine in the culture medium Furthermore, a lysine analogue (S-aminoethylcysteine) resistant mutant was obtained as an l-lysine producer and in this strain aspartokinase was insensitive ... world in 1996 Amino acid Method Japan Glycine l-Alanine dl-Alanine l-Aspertate l-Aspergine l-Arginine and analogs l-Cysteine and analogs l-Glutamate l-Glutamine l-Histidine l-Isoleucine l-Leucine...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
Ngày tải lên :
16/03/2014, 16:20
... Amino acids at positions 3, and are in bold type Protein Ca2+ binding/EF hand proteins Aplycalcin Hippocalcin Neurocalcin Rem-1 Visinin-like protein ADP-ribosylation factor Arf-6 Fig The combination ... positions and 6, in protein N-myristoylation were evaluated by metabolic labeling of model substrate proteins in an in vitro translation system In this case, to avoid the effects of amino acids at other ... critical determinant for protein N-myristoylation In this study, to examine the effect of the combination of amino acids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
Ngày tải lên :
17/03/2014, 10:20
... AR3)36 subdomain AR3)13 in N/C interaction and the role of individual amino acid residues in and flanking the 23 FQNLF27motif in AR16)36 in N/C interaction Yeast protein interaction assays indicated ... Alanine scanning of AR17–32: amino acids flanking F23, L26 and F27 modulate AR N/C interaction (A) GalAD-ARpeptide fusion proteins in the yeast protein interaction system (B) Interaction of GalAD-ARpeptides ... (C) Western analysis of indicated GalAD-AR.NTD proteins in the yeast protein interaction system (left panel) and of indicated AR.NTD proteins in the mammalian protein interaction system (right...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... of 9.7) Thus, the background in the off-state was much lower with pEBTetD than with pEBTet However, in contrast to expression of membrane proteins, the level of expression of cytosolic eGFP in ... results for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference experiments, ... of inducible protein expression M Bach et al Fig Analysis of eGFP expression in 293 cells by flow cytometry 293 cell pools stably transfected with either pEBTet ⁄ eGFP or pEBTetD ⁄ eGFP were cultured...
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Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx
Ngày tải lên :
30/03/2014, 04:20
... Residues involved in the binding to the PLP moiety are shown in blue, whereas those involved in protein protein interaction are shown in green The first four amino acids of the barley serine racemase ... requiring the C-terminal binding motif [30] Protein interacting with C kinase (PICK1) contains one PDZ domain that is required for interacting with protein kinase C (PKC) [30,31] or serine racemase ... PDZ interactions with the C-terminal amino acid triplet of serine were crucial for binding, but instead, the interaction was established with its N-terminal 66 residues Binding of Golga3 increases...
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Báo cáo y học: "A computational method to predict genetically encoded rare amino acids in proteins" pdf
Ngày tải lên :
14/08/2014, 14:22
... Sections 4of the multiple sequence alignments of the putative pyrrolysine-containing proteins Sections of the multiple sequence alignments of the putative pyrrolysine-containing proteins (a) A protein ... special subset of redox proteins in which one of the cysteines is replaced with a selenocysteine Consistent with earlier reports [13,23], a set of selenoproteins was identified in a group of methanogenic ... strictly contingent on the presence of a protein homolog containing a cysteine substitution in the database or on a canonical SECIS motif in the case of selenoproteins In addition to almost all of the...
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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation
Ngày tải lên :
02/09/2015, 13:46
... Iron(III) and copper(II) complexes bearing 8-quinolinol with amino- acids mixed ligands 743 aeruginosa (ATCC27853) of 43 mm at 10 lg/ml signalling its potential as pharmaceutical or chemotherapeutic ... coordination compounds of mixed ligands such as benzoheterocyclic rings and amino acids have been the focus of a considerable number of investigations for their good coordination ability with metal ions, ... solution of 0.81 g FeCl3 (5 mmol) and (0.725 g, mmol) 8-hydroxyquinoline in 20 mL methanol, the amino acid (0.445 g alannine (ALA) or 0.746 g methionine (MET) that is mmol) was added with constant...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Ngày tải lên :
07/03/2014, 15:20
... decrease of more than 80-fold in the secretion of recombinant protein was associated with the deletion of the C-terminal part(s) of the protein, even though the intracellular expression levels remained ... selected by culture of the cells Cycling conditions 30 s/94 °C; min/57 °C; min/72 °C min/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C min/94 °C; min/54 ... °C; min/72 °C min/94 °C; min/57 °C; min/72 °C min/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C 30 s/94 °C; min/57 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C 30 s/94 °C; min/56...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... of many proteins involved in apoptosis, including the expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis ... Protein expression: ↑ means increased expression of protein ↓ means decreased expression of protein → means no changes of protein expression means negative expression mRNA expression: (+) means positive, ... expression of Bcl-2 and by modulating phosphorylation of JNK Table Altered protein expression of JAR cell with anti-Bag-1 antisense oligo nucleotide induced by hypoxia in the apoptosis pathways using...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
Ngày tải lên :
15/02/2014, 01:20
... administration of exogenous dopamine in the culture medium [7,13–15] Results Dopamine increases the expression of a-synuclein to a threshold To obtain a cellular model of a-synuclein overexpression, ... [9,10] In the present study, we investigate the dopamine effect on the expression pattern of cellular proteins in the human catecholaminergic neuroblastoma cell line SH-SY5Y, overexpressing a-synuclein ... affected the levels of four proteins [stathmin 1, glutathione S-transferase 4912 d Not applicable (GST)p, Ran binding protein and C1q binding protein] , related to cell signaling, apoptosis and cytoskeleton...
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báo cáo hóa học: " Participation of MCP-induced protein 1 in lipopolysaccharide preconditioning-induced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines" pdf
Ngày tải lên :
19/06/2014, 22:20
... Participation of MCP -induced protein in lipopolysaccharide preconditioninginduced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines Jian Liang 1* , Jing Wang 1, ... MCP-1 -induced protein attenuates endotoxin -induced myocardial dysfunction by suppressing cardiac NFкB activation via inhibition of IкB kinase activation J Mol Cell Cardiol 2011 May 17 [Epub ahead of ... protein induced protein (MCPIP1), a recently identified CCCH Zn finger-containing protein, plays a role in focal brain ischemia and to elucidate the mechanisms of LPS -induced ischemic brain tolerance...
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Báo cáo y học: "Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specime" pot
Ngày tải lên :
09/08/2014, 06:23
... analyzed gene -expression profiles in the synovial lining tissues selected by LCM in a cDNA microarray by comparing those in multilayered lining tissues with those in normal-like lining tissues in each ... evaluated in accordance with established criteria [14], with modifications involving the degree of proliferation of synovial cells, typical palisading of synovial cells in the intimal lining layers, ... multilayered lining Laser capture microdissection of of synovial lining regions with normal-like lining or multilayered lining (a,d)before microdissection; (b,e) after tracing around the lining regions...
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Báo cáo y học: "Effect of infliximab on mRNA expression profiles in synovial tissue of rheumatoid arthritis patients" ppsx
Ngày tải lên :
09/08/2014, 08:23
... potential of using gene expression profiling to elucidate the effects of anti-TNF treatment in RA patients using synovial biopsies and indicate the possibility of identifying gene expression ... planning and design of the project and participated in both the data analysis and writing of the article JL was involved in project design, analysis of laboratory results, data analysis and writing ... Discussion This investigation was a pilot study of the feasibility of using gene expression profiling to predict responses to TNF-blocking and to assess the effects of TNF-blocking on gene expression...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
Ngày tải lên :
12/08/2014, 03:20
... processing enzyme family protein ATHB-1 (Homeobox-leucine zipper protein) C2 domain-containing protein C2H2 type zinc finger family protein C3H4-type zinc finger (RING finger) protein CBS domain-containing ... trafficking, etc The second group was comprised of the genes of G protein (Transducin, GTP binding protein) and AMP-binding (Adenosine monophosphate binding) protein, which are involved in signal ... domain-containing protein DnaJ protein, putativeb Ethylene responsive element binding protein (EREBP) Eukaryotic elongation factor 1A F-box domain containing protein, putative GTP-binding protein, putative...
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Báo cáo y học: ":Identification of novel stem cell markers using gap analysis of gene expression data" ppsx
Ngày tải lên :
14/08/2014, 08:20
... factors uncoupling their expression Rab family of GTPases: Rab3d The Rab family of small GTPases are involved in intracellular cell signaling processes, including tethering and docking of vesicles ... suppressors In contrast to eukaryotes, prokaryotic serpins are rare and most serpincontaining prokaryotes have only a single serpin gene [24] In agreement with our findings, variation in serpin gene expression ... set of 12 genes, five of which belonged to the family of serpins (SERine Protease INhibitors; serpins a1b, a3n, b9, g1, and h1) Serpins are a large class of proteins that are found in all multicellular...
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Báo cáo y học: "Genomic analysis of metabolic pathway gene expression in mice" pps
Ngày tải lên :
14/08/2014, 14:21
... copper-containing (Aoc3) (12.5-fold change in expression level) This observation is consistent with the study in which overexpression of human AOC3 in mice resulted in an increase in weight and ... identified here In the analysis reported in Schadt et al [6], the loci on chromosomes and 19 were associated with distinct subsets of mice, identified by differing expression profiles of the most ... was determined empirically by performing the analysis after random permutation of the grouping assignment of the mice, with the probability of falsely rejecting the null hypothesis determined by...
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Báo cáo y học: "cGlobal analysis of microRNA target gene expression reveals the potential roles of microRNAs in maintaining tissue identity" ppsx
Ngày tải lên :
14/08/2014, 16:20
... findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and particularly in maintaining tissue identity rather than in determining or regulating ... These findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and maintaining tissue identity rather than in determining or regulating tissue-specific ... understanding the global role of miRNAs in maintaining lineage of differentiated tissues and cells has great impact on the studies of miRNAs in cancer genetics 16 Methods Datasets used in this...
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