split horizon is disabled by default on frame relay physical interfaces
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... kDa band was excised. Thesample was analysed using Q-TRAP MS by the ProteinAnalysis Laboratory at the Cancer Research UK LondonResearch Institute (London, UK).ImmunoprecipitationCells were ... was clarified by centrifugation at15 000 g for 30 min followed by centrifugation at 100 000 gfor 1 h. Saturated ammonium sulfate solution (200 mL)was added (33% final concentration) and incubated ... phosphorylation of CDK4 on Y17 is mediated by Src family kinases.ResultsDetection of CDK4 Y17 phosphorylation andkinase activityThe N-termini of CDKs 1, 2, 4 and 6 are highly con-served and there is...
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Moment and Shear, Generated by DL on the Composite Section
... Generated by DL on the Composite Section.This generator is capable of handling from 1 to 10 spans, and is capable of returning values for continuous sections. This is done by moment distribution. ... span pointcolumn 1 = momentcolumn 2 = shearBased on continuous section, constant inertia.1 1.2 1.4 1.6 1.805001000mcn81n8sp+disp0 1 201234567891011121314151617181920212223242526272829303132333435363738391 ... of the design lane.Combination 3: For both the negative moment between points of contraflexure under a uniform load on all spans, and reaction at interior piers only.90% of two design trucks...
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KRONE - What is Patch by Exception
... of tomorrow.WHAT IS PATCH BY EXCEPTION?Patch by Exception is a product feature of KRONE's HIGHBAND 8 and 10 modules that allows the normal cross connect configuration to be wired using ... in configuration is required the new connection can be simply made by plugging a HIGHBAND patch cord into the module to instantly set up the new configuration by overriding the jumpered connection. ... progressive connectivity company is introducing "Patch by Exception", the next generation in cable management systems.Patch by Exception guarantees network managers a system that is not only...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
... corre-sponds to the 40 kDa form, consists of the N-terminalregion (N) and one Kunitz domain (K1), whereasHAI-1–NK1LK2, corresponding to the 58 kDa form,consists of the N-terminal region (N), ... separated by SDS ⁄ PAGE under reducing conditions (B) or under nonreducing conditions (C), and analysed by immunoblotting with the anti-c-Myc IgG. The protease domain of TMPRSS13 was quantified by scanning ... tissuesproducing it. In response to tissue injury, such ashepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively inthe injured tissue. This conversion...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... followed by Strep-tag IIwas isolated by BamHI digestion and inserted into theBamHI site of pmGFP–calpain 7.To construct pCMV3xFLAG–IST1 and pGEX–IST1, anIST1 cDNA fragment was amplified by PCR, ... intermolecular reac-tion occur in the autolysis. Therefore, it is prematureto draw conclusions regarding the mechanism of theenhancing effects of IST1 on mGFP–calpain 7–Strepautolysis in vitro.Both ... culturedcells. On the other hand, overexpression of FLAG–IST1DMIM1,2suppressed the autolysis. This observationmight be explained by regarding IST1 as a regulator ofthe intracellular localization of...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... allincubation conditions. When rhTLK1 is diluted fromhigh storage concentrations to low assay concentra-tions of 23 ngặmL)1, which is below the limit forthe ATP tetramerization effect, the ... respectively, depending on pre-assay incubation with ATP. Here,the part of ATP that is necessary for tetramerization and how the reactionvelocity is influenced by the enzyme concentration are determined. ... scintillationcounting.Analysis of kinetic dataKinetic data were fitted by non-linear regression analysis tothe MichaelisMenten equation v ẳ VmaxẵS=KmỵẵSịor the Hill equation v ẳ VmaxẵSn=K0:5nỵẵSnị...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
... light on thefunction of this interaction in the in vivo situation. By influencing the duration of the Ca2+signal ofORs in the cilia of the sensory neurons, MUPP1 couldhave a strong influence on ... interaction wasdetected using MUPP1 antibodies.ImmunohistochemistryMice were raised and maintained according to governmen-tal and institutional care instructions. Immunohistochemis-try was ... proportion of transiently transfected cellsresponding to odorant in ratiometric calcium imagingexperiments [24–26]. This alteration in the subcellulardistribution of MUPP1 upon co-expression with...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... formation by transferring electronsfrom NADPH onto oxygen molecules [56]. A defici-ency of one PHOX subunit in NADPH oxidase leadsto the inhibition of superoxide generation, and resultsin chronic ... cytokine synthesisand febrile response induced by SEC1 is dependent on NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK ... localization of epitopes on tryptic fragments. Infect Immun 45, 281–285.5 Cremonesi P, Vimercati C, Pisoni G, Castiglioni B,Luzzana M, Ruffo G & Moroni P (2006) Identificationof enterotoxin genes...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
... were isolated as a single peak fraction by a 0.1–1.0 m NaCl linear gradient in R buffer. Histone H1,which exists in the DNA affinity fraction, was separated asa major component stepwise elution ... histone H1 [14],topoisomerase II [15], lamin B1 [16] and SAF-A(hnRNP U) [17].It is known that the aggregation of DNA is com-patible with the functions of proteins under in vitroconditions. ... and one of thecandidates may be linker histone H1 of an aggregativeprotein. IR provokes ATM-dependent dephosphoryla-tion of histone H1 [42]. A recent study demonstratesthat whereas histone...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... mmTris ⁄ HCl (pH 7.5). After incubation for 1 h at 4 C,20 lgặmL)1of heparin was added and the incubation wascontinued for 5 min. The complex formation was analysed by electrophoresis on nondenaturing ... protein containing a fused N-terminal histidine tagwas purified by binding to and elution from Ni-NTA-affinitymatrix [43].Nuclear extract preparation and fractionationof the B. mori transcription ... mutual contamination. Inevery fractionation the quantities of fractions had tobe optimized because use of larger amounts of anyindividual fraction tended to result in inhibition oftranscription....
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... intranscriptional control and their impact on cellular function.Oncogene 19, 2468–2473.45. Tone, M., Powell, M.J., Tone, Y., Thompson, S.A. & Waldmann,H. (2000) IL-10 gene expression is controlled ... prothrombinase. This combination of consti-tutive expression and induction may permit a rapid responseto inflammation. However, the mechanisms regulating fgl2expression, either constitutive or ... demonstrated thatFig. 7. EMSA analysis of the function of Ets/Stat3 cis elements. (A) Cross competition of consensus Ets/PEA3, Ets/PU1, and Stat3 cis elementswith corresponding transcription...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... for a region of this constructcontaining galA and some of the flanking regions,resulting in the genomic sequence of A. niger galA.Thepresence of one putative intron was confirmed by RT-PCR ... start codon. In addition, one putative PACCsite ()549) [31] and two CCAAT sites ()280, )300) weredetected.Expression analysis ofgalAThe expression pattern of galA on a selection of mono-saccharides, ... library of A. niger usingthis fragment as a probe resulted in the isolation of twogalA-containing phage k clones. From one of these clonesa4.5-kbEcoRI fragment was cloned into pBluescriptSK+,...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... metal.In all structures solved for the mononuclear SODs, themetal ion is held in place by an absolutely conservedquartet of residues comprising three histidines and oneaspartic acid which act as ligands ... noother SODs with this mutation exist naturally. The natureof this phenomenon still remains elusive.Metal specificity may be considered as consisting of two,presumably distinct, stages. First, ... peroxide on the iron-containing superoxide dismutase of Escherichia coli.Biochemistry 26, 1251–1257.47. Misra, H.P. & Fridovich, I. (1978) Inhibition of superoxide dis-mutase by azide....
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... suggested thatLBR binds to a DNA region on chromatin in the RS region.This idea was consistent with a report by Ye & Worman[15], i.e. that a region corresponding to RS binds free DNA.Duband-Goulet ... chromatin is regulated by phosphorylation in the arginine-serine repeat-containing region (RS-region) by multiple kinases. Thepotential function of LBR and LAP2 in vesicle targeting tochromatin is ... Expression of fusion proteins was induced by the addition of 0.1 mMisopropyl thio-b-D-galactoside,followed by incubation for 6 h at 30 °C. The bacterialcells were collected by centrifugation...
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