... the
quantitation of microgram quantities of protein utilizing the
principle of protein-dye binding. Anal. Biochem. 72, 248–254.
20. Laemmli, U.K. (1970) Cleavage of structural proteins during the
assembly of ... protein belonging to the GTP-
binding elongation factor family, which promotes the GTP-
dependent binding of aminoacyl-tRNA to the A-site of
ribosomes during protein biosynthesis and the capture of
deacylated ... quantify the amount of recovered
protein owing to the presence of a high molar excess of
BSA remaining in the buffer. Therefore, a fixed aliquot of
the protein was incubated with the indicated probes,...
... category of
symptomatics.
DISCUSSION
The findings of the three surveys showed
the relative importance of cough as a predominant
symptom employed in screening the population. In
fact, two screening ... weeks or more as defined in RNTCP. This
indicated the importance of identifying symptomatics
employing cough of two weeks or more instead three
weeks or more for diagnosis of TB. Among the
55561 ... patient
having cough of three weeks or more with or
without other symptoms. The importance of
including quality check in the survey employing
symptom inquiry was well demonstrated in an earlier
report
12
...
... of increase in LV dyssynchrony during
dobutamine stress in patients with wide QRS duration
(20). In contrast, Kurita et al. reported on an increased
mechanical dyssynchrony during pacing–induced ... time in IDCM
patients yielded the following findings: LBBB was a
major determinant of the duration of LV systole, irre-
spective of the presence of left ventricular disease. The
effect of the ... hemodynamics
The patients performed supine bicycle exercise
beginning at a load of 25 watt with increases of 25 watt
every 5 min
until the development of fatigue or
limiting dyspnoea
. Rest and exercise...
... were interrupted by two
introns located in identical positions (Fig. 1B). The first
intron included nucleotides 50–96 and the second intron
included nucleotides 210–253 in each gene. Excluding
introns ... longitudinal interactions formed by c-T1 and c-T2.
Finally, c-T2 possesses a glycine at position 72 in place
of consensus ⁄ c-T1 arginine (Fig. 4B¢). This substitution
may ‘open’ the nucleotide-binding ... whereas the level of the c-T1 transcript
remained unchanged. Ninety-eight percent of the cells
were undergoing mitosis ⁄ cytokinesis at this time (as
determined by counting of cells using a stereomicro-
scope)....
... and
consisted of a linker (Glc-Kdo
2
)-lipid A structure. The
phenotype of this mutant was examined to investigate
the roles ofdifferent moieties of the LOS molecule in
biological activities of the ... (10 lLof10
6
colony forming units, CFU) were inoculated into 190 lL
reaction wells containing the diluted normal human serum,
25% of heat-inactivated normal human serum, or DPBSG
alone and incubated ... chromosomal DNA
of M. catarrhalis strain O35E using primers 25 and 26
(Table 1, Fig. 2). The PCR product was cloned into
pCR2.1 using a TOPO TA cloning kit (Invitrogen, Carls-
bad, CA) to obtain pCRlgt3....
... Co-stimulation of
T cells with CD2 augments T CR-CD3-mediated activation of
protein tyrosine kinase p72syk, resulting in incresed tyrosine
phosphorylation of adapter proteins, Shc and Cbl. Int. Immunol.
10, ... containing th e GAL4 DNA-
binding domain and the c-Ju n activation domain (residues
1–223) in wild-type and mutant forms ( not phosphorylat-
able by mutation of both serines 63 and 73 to alanine).
These ... f
these protein interactions, tetraspanin antigens can in uence
intracellular signalling pathways. The ligation o f CD53 has
been shown to induce intracellular calcium mobilization in
different...
... of microgram quantities of protein
utilizing the principle of protein-dye binding. Anal
Biochem 72, 248–254.
40 Laemmli UK (1970) Cleavage of structural proteins
during the assembly of head of ... observed in the
case of the superimposed PKV–RKV models, whose
major nonoverlapping regions are, instead, localized in
the H-site of the enzymes (Fig. 2B). These findings are
in agreement with the different ... the
maintenance of a high-affinity CDNB-binding site. However, the substitu-
tion E117K gives rise to mutants showing increased k
cat
values for
CDNB, suggesting that Lys117 might positively in uence...
... importin b binding (IBB)
domain in its N-terminus and the C-terminal NLS-
binding domain. In the absence of importin b,an
auto-inhibiting part of the IBB domain forms an intra-
molecular interaction ... peptide of the cargo binds
in extended conformation to the binding pockets of
the superhelical surface of importin a. These binding
pockets contain several conserved residues (e.g. aspara-
gine, ... oxygen
atoms of conserved amino acids within importin a (the
main-chain oxygen of glycine at position 168, the side-
chain hydroxyl oxygen of threonine at position 173, and
the side-chain carboxylate...
... such as vitamin A, vitamin E diminish the lipid
content of blood plasma in patients with non-insulin-dependent
diabetes mellitus (Lee et al., 2007a). The evaluation of GABA in
germinated brown ... Administration,
Republic of Korea.
References
Abdullah, A., Baldwin, R. E., & Minor, H. (1984). Germination effect on flatus causing
factors and antinutrients of mungbeans and two strains of ... becomes one of the focuses in the field of func-
tional food research. Change in the GABA content is enhanced in
the germination state, so allowing time for germination during
processing can help...
... microdomains (GEM) of the plasma membrane
and to be involved in the regulation of epidermal growth
factor receptor (EGFr or ErbB1) activity. To gain further
insight into the mechanisms involved in ... masses) of recombinant proteins are indicated.
(B) Protein profile of the gradient, visualized by Ponceau S staining of
the nitrocellulose membrane.
Ó FEBS 2004 EGF receptors distribution in cell ... microscopy
CellsgrownincoverslipswerewashedinNaCl/P
i
,fixedin
acetone at )20 °C for 7 min, and incubated in NaCl/P
i
containing 3% (w/v) BSA
4
for 1 h at 37 °Ctoblock
nonspecific binding sites. Coverslips...
... θ of an individual at a given point of time in terms of the
prevailing age t, individual background characteristics x, current conditions z(τ),
exogenous business-cycle indicators c(τ − t) of ... probability
of twinbirths (see Subsection 4.1.2) is in line with our finding that twinning rates
are slightly lower in recessions.
18
Some other possible explanations seem less likely. If inferior housing ... Press,
Durham.
35
1 Introduction
In many scientific disciplines, the interest in long-run effects of early-life con-
ditions has been strongly increasing during the past years. In medical science,
the...
... modifica-
tions of the side chains of amino acids within a protein. In
particular, the side chains of cysteine, histidine, methionine,
tryptophan and tyrosine are susceptible to ROS-induced
modifications ... (1999)
Photodynamic crosslinking of proteins. Kinetics of the FMN-
and rose bengal-sensitized photooxidation and intermolecular
crosslinking of model tyrosine-containing N-(2-hydroxypropyl)
methacrylamide ... 4A,B).
Matching the blot with the protein map shows that PDT
caused labeling of a-tubulin and b-tubulin (spots 1 and 2).
The minor spot 3 probably reflects labeling of a small
fraction of actin. The...
... importance of the positioning
in the membrane. When changing the two tryptophans
(residue 48 and 56) of penetratin to phenylalanines it was
shown that the translocating property of penetratin was
essentially ... differ-
ence in chemical shift observed for Phe49 in penetratin and
in penetratin(W48F,W56F) is most likely due to changes in
ring currents when changing amino acid 48 from a
tryptophan to a phenylalanine, ... average size of the head-group region of the bilayer. Only
effects that result in a remaining amplitude of <0.4 are shown. Blue
color indicates the effect of Mn
2+
ions, light red the effect of
1-palmitoyl-2-stearoyl-sn-glycero-5-doxyl-3-phosphatidylcholine,...
... E.
(1994) Binding sites of quinones in photosynthetic bacterial
reaction centers investigated by light- induced FTIR difference
spectroscopy: binding of chainless symmetrical quinones to the
Q
A
site of ... (1994) Binding sites of quinones in photosynthetic bacterial
reaction centers investigated by light- induced FTIR difference
spectroscopy: assignment of the interactions of each carbonyl of
Q
A
in Rhodobacter ... stretching vibration of
Q
A
by 60 cm
)1
[10,11], indicating strong asymmetric
binding of UQ
10
at the Q
A
site, in contrast to symmetric,
weaker binding of UQ
10
at the Q
B
site [12,13].
To explain...
... identifi-
cation of two light- dependent protein kinases involved
in phosphorylation of thylakoid proteins. STN7 pro-
tein kinase is essential for phosphorylation of Lhcb1,
Lhcb2 and Lhcb4 proteins [11,17] ... conditions, including the process of leaf senes-
cence in Arabidopsis [31].
The C-terminus of CaS contains also a motif for
interaction with 14-3-3 proteins and FHA domains,
according to eukaryotic linear ... motif
that is involved in interaction with 14-3-3 proteins and
proteins with the ‘forkhead-associated’ (FHA) domain.
These domains are found in a variety of signaling pro-
teins, and can bind directly...