solidstate nmr spectroscopy on complex biomolecules
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
Ngày tải lên :
19/02/2014, 12:20
... a,b-anor-
meric configuration. However, because of the complexity
of overlapping signals of the same monosaccharide
residues in a very similar chemical environment, it is
difficult for NMR alone to assign ... [17], one trifucosylated [23], one tetrafucosyl-
ated [24], and one pentafucosylated [24] oligosaccharides.
Acidic structures described include one monosialyl-mono-
fucosylated [25], two monosialyl-difucosylated ... gel-filtration and ion-exchange columns with distilled
water. The partially resolved octasaccharide to undeca-
saccharide fractions were further fractionated by normal-
phase HPLC on a preparative Separon...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
Ngày tải lên :
20/02/2014, 02:22
... 0.5%.
1
H nuclear magnetic relaxation dispersion (NMRD) pro-
files were recorded at variable concentration of myristate
by measuring water proton longitudinal relaxation rates
(R
1
obs
) at magnetic ... con-
centration of the paramagnetic metal ion, and T
1M
is the
longitudinal relaxation time of localized water protons
[20,36].
Relaxivity of Mn(III)heme–HSA solutions at 25.0 ° Cas
a function ... 46724683 ê 2005 FEBS 4681
Contributions to relaxation differ, depending on the
conformational state of HSA and on the occupancy of
the myristate binding sites. The relaxation rate change
is highest...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
Ngày tải lên :
08/03/2014, 10:20
... experimental
conditions, d espite a r elaxation delay of 4.3 s and a satu ration
time of 3 s. Nevertheless, saturation transfer to the N-methyl
groups was not observed under these conditions. A satura-
tion ... legionaminic acid was 1 : 1 in long-chain and 1 : 2 in
middle-chain PS
NH
4
OH/HF
, respectively. Based on the rela-
tive intensities of the proton signals it was concluded that
only one legionaminic ... exchange could be observed
under the conditions applied. From these data it is conclud ed
that under certain conditions it is possible to observe the
interconversion of the cis and trans N-methyl...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... (1991) Assignments of backbone
1
H,
13
C, and
15
N
resonances and secon dary structure of ribonuclease H from
Escherichia coli by heteronuclear three-d imensional NMR spec-
troscopy. Biochemistry ... expression systems based on Escheri chia coli extracts
[2,10,15,16]. At these yields, protein concentrations are
sufficiently high t o allow the recording of NMR spectra
without further concentration ... gene, using a combination of ligation and
recursive overlap extension of complementary synthetic
oligonucleotides as described above. Oligonucleotides used
for the construction of the s-CYPA are...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
Ngày tải lên :
17/03/2014, 10:20
... typical
crystallization conditions comparing the E-andthe
Z-configurations are demonstrated in Fig. 3. Despite the
identical crystallization conditions, only the E-configuration
crystallized (Fig. ... fraction (Fig. 1),
only the two naturally occurring PEC-linker complexes are
present, confirming the brief dissociation and separation
conditions [4]. The second important preparation step was
that ... Z-and
E-configurations of one chromopeptide with an excellent
solubility and stability were investigated by 1D NMR
spectroscopy. The information presented here are mainly
focused on the preparations,...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
Ngày tải lên :
23/03/2014, 04:21
... transition under
conditions of slow exchange on the chemical-shift timescale,
the dissociation constant, K
d
, is given by
K
d
ẳẵPẵL=ẵPL
Here, [P], [L] and [PL] are the respective concentrations
of ... exchange regime. Thus, the
configuration at the hemiacetal carbon of galactose
may affect the dissociation constants.
By contrast, because chemical-shift changes upon
the addition of sugars at subdomain ... pulses for saturation with
1 ms intervals and a total saturation time of 2 s. On- res-
onance irradiation of the protein was conducted at a chemi-
cal shift of –0.4 p.p.m. and off-resonance at a chemical
shift...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot
Ngày tải lên :
23/03/2014, 13:20
... NTX-1
were confirmed by electron spray ionization/mass spectro-
metry (ESI-MS).
NMR spectroscopy
NTX-1 was dissolved in D
2
O/H
2
O (10 : 90, v/v) pH 3.2 or
99.9% (v/v) D
2
O to the final concentration ... flexibility [37]. On the other hand,
the substitution o f S er in other long neurotoxins with G ly33
in NTX-1 (Fig. 1) at the tip portion of loop II makes this
region conformationally more variable.
Binding ... possible explan-
ation for the lower dissociation constants of long neuro-
toxins from nicotinic acetylcholine receptor c ompared to
short ones.
Fig. 6. Comparison of NTX-1 NMR and 1NTN X-ray...
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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
Ngày tải lên :
23/03/2014, 18:20
... expression
of downstream genes, as appropriate. The expected con-
sumption of phenylacetate was confirmed by HPLC of
the culture fluid using online liquid-scintillation counting
for detection (data ... strains grew on succinate, but none
grew on adipate.
Transformation of labeled phenylacetate into labeled
products by the mutants
Mutant cell suspensions were incubated in buffer contain-
ing ... carbon atoms (122.1, 123.1, 126.5 and
129.3 p.p.m.; Table 1); they were all correlated to directly
attached protons as shown by 2D HMQC spectroscopy
(Fig. 4). Carbon connectivities established on...
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Báo cáo khoa học: Macromolecular NMR spectroscopy for the non-spectroscopist pot
Ngày tải lên :
29/03/2014, 00:20
... deviations of bond lengths,
bond angles and dihedral angles from ideality, unfa-
vourable sidechain rotamers, and bad nonbonded
interactions. molprobity additionally offers all-atom
contact analysis ... ‘resolution’ of NMR- derived protein structures.
Assessment criterion Very high resolution High resolution Medium resolution Low resolution
Restraints per residue
a
> 18 14–18 10–15 < 10
Backbone ... macromolecular NMR spectroscopy,
including basic interpretation of commonly encoun-
tered NMR spectra. We then outline the process by
Keywords
HSQC; nuclear magnetic resonance (NMR)
spectroscopy; ...
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Báo cáo khoa học: Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination potx
Ngày tải lên :
29/03/2014, 00:20
... on the existence of motions on the ps to
ns timescale, whereas R
2
additionally depends on
slower motions on the ls to ms timescale. These data
can be analysed [41–43] to separate contributions ... VTS1p–RNA interac-
tion is driven by an increase in conformational entropy.
Slower motions (ls-ms)
As noted above, R
2
relaxation rates can provide infor-
mation on slower internal motions in proteins.
Related, ... and
such motion can occur on timescales ranging from pico-
seconds to seconds or more.
Fast motions (ps–ns)
These motions are accessible through the measurement
of the relaxation rate constants for...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
Ngày tải lên :
30/03/2014, 20:20
... [3], the
active conformation of EM2 should be taken into con-
sideration on the basis of not only the conformational
difference in the backbone chain but also the overall
conformation, including ... to C-terminal amidation.
Solution conformation by NMR spectroscopy and
simulated annealing calculation
The conformational features of EM2 and EM2OH,
obtained by the present NMR measurements and
molecular ... docking
models were constructed for the open conformers and
subjected to energy evaluation for reasonable configur-
ation and translation exploration using the Docking
module of insight discover. Consequently,...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
Ngày tải lên :
31/03/2014, 21:21
... function is unknown, it is
dependent on an interaction with the nonsecreted TTSS
chaperone LcrH [12,13]. This interaction is responsible for
the presecretory stabilization and efficient secretion ... determination of a TTSS chaperone/
effector protein complex from Salmonella spp., it is likely
that at least one function of chaperones is to maintain their
cognate partner in an elongated unfolded ... application to proton correlation spectroscopy.
J. Magn. Reson. 53, 521–528.
25. Jeener, J., Meier, B.H., Bachmann, P. & Ernst, R.R. (1979)
Investigation of exchange processes by two-dimensional NMR
spectroscopy. ...
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