... the large P6extension andthe last three nucleotides (AU XG) ofthe intron. The sizes ofthe intron (InDGb) and 5¢ exon (5E) are indicated. The arrowindicates cleavage ofthe pre-RNA at the ... 23S.5DGbpre-RNA, andthe binding of a minimum of n Mg2+ions leads to formation of the active complex, whereas binding of a minimum of mMn2+ions forms the inactive complex. The sizes of the active and ... Because of these and other properties of Cr.LSU, the tertiary structureofthe intron from23S.5DGbwas examined using Fe2+-EDTA cleavage. The ground-state structure shows evidence of an unusually...
... the (door) the (ladder) the (planet) the (woman) her (arm) It she the (man) we the (air) It them their they the (plants) they they their the (man) the (controls) he I the ... them there is a spaceship. There are two astronauts’ helmets, one in front of them andthe other at their back. They are holding a fruit in their hands. The woman is looking at the man. The ... sources, the third states the phoric status ofthe tie, andthe last presents the chain relation in terms of the address of all the preceding members in the chain. Tables 6 and 7 summarise the...
... the structural and functional importance ofthe v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neu-rons and cerebellar granule cells. Further ... and 482) and one Thr residue (amino acid 487) were well conservedin the KIND2 of v-KIND in all species analyzed,although not in the other KINDs. To investigate the possible involvement of these ... total size) is not yet fully under-stood, the data obtained in the present study indicatethat the 43 residues (amino acids 702–744 in mice) thatreside in the middle region ofthe CD act as the v-KIND...
... b-sheet and loopsconnecting these secondary structure elements. The con-tents ofthe a-helix and b-strand were 50% and 11%,respectively. The monomer structure comprised twodomains: the rod-shaped ... metal cofactor.Figure 3D shows the superimposition ofthe activesite structures of apo, Mn-bound and Fe-bound Ape-SODs. The most significant difference among themwas related to the OH of Tyr39, ... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 andthe NE2 of His169, formed anequatorial plane (Fig. 3B). The metal...
... especiallythosewhoholdgeneralbachelor’sdegrees,theirqualificationsserveonlyasasignal of theirpotentialtoemployers.Inmanycases,theytakelongertorealiseemployment and whentheydotheirentryleveljobsdonotnecessarilyrequire the years of schoolingtheypossess.Theystartat the bottom ofthe jobladder and havetoprovethemselvesin the labourmarkettoreachhigherincomelevels.Acombination of theirpotential and trainingwilldeterminetheirprogressionup the ladder.Aswillbeseenin the nextsection, the field of study,whichsignals the type of skillsacquiredthrougheducation,playsasignificantroleinemployability of universitygraduatesinthiscase.PercyMolekeFree ... www.hsrcpress.ac.za4PercyMoleke5InequalitiesinHigherEducation andtheStructureofthe LabourMarketDespite the observedsignificantincreasesin the number of seniorcertificateholdersin the pastdecade, the proportion of learnerswhoactuallysitfor the examremainslow. The quality ofthe seniorcertificateexamalsocontinuestobe of concern. The largemajority of learnersstillopttowrite the seniorcertificateexamon the standardgraderatherthanon the highergrade.Consequently, the proportion of thosepassingwith ... to theirAfrican and colouredcounterparts. The proportion of white and Asiangraduateswithineachcategory of number of yearsworkedwasnotashighasthat of otherracegroupsat the time ofthe survey(Table8).Table8:Number of yearsworkedbythoseinmanagement,byraceNumber of yearsworkedAsian...
... splittingconstants of the 15N nucleus ofthe distal NO and of the 14N nucleus ofthe proximal His ofthe GmHO-1complex are closer to those ofthe rHO-1 complex thanto those ofthe SynHO-1 complex. Thus, ... analyses of the molecular features ofthe heme–GmHO-1 complex and ofthe mechanism of heme degradation were per-formed, andthe results were compared with those for the heme complexes of SynHO-1 and ... (B) and 25 K for (C)–(E).(A) The ferric heme complex of GmHO-1 at pH 7.0 (0.1M, KPB).a-1, Expansion ofthe gxyregion of (A) (solid line) andofthe corres-ponding part ofthe spectrum of...
... confirming that the mutation of Tyr186 to Phe186 does not grossly perturb the overall framework ofthe enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA ... resolved and are boundbetween the progesterone andthe protein (Fig. 7A).In comparison to the oxidized structures, the FMNisoalloxazine ring is less planar, with both the N5atom andthe C8-C7 ... and indicate re-oxidation ofthe flavin andthe formation ofthe hydride-Meisenheimercomplex of TNT. (C) As for panel B except the spectral changes are recorded over an extended time period and...
... SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment ofthe HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... against the hemocyanin fraction showstaining ofthe two 75 kDa bands in Western blot, but donot recognize any other proteins ofthe hemolymph.Although we expected that each of these bands containstwo ... primers andthe T3 vector primer. The sequences were obtained after the cloning ofthe PCRproducts into the pCR4-TOPO TA vector (Invitrogen).Protein structure modelingHomology models ofthe S....
... migrating bands of three LPS(without R14) and fast migrating bands of P. mirabilisG1, O 28, and R14 LPS (Fig. 5A). These bands correspondto high- and low-molecular-mass LPS species consisting of the ... migrating bands of allLPS tested and slow migrating bands of P. mirabilis S1959,G1, and O28 LPS, the banding patterns being clearlydifferent (Fig. 5B). These findings together showed that the epitope ... O-polysaccharide p art of LPS. The serological relatedness of P. mirabilis G1 and S 1959correlated with the similarity ofthe chemical structures of their O-polysaccharides (Fig. 3). Therefore, it is...
... region of the 1H,15N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states ofthe protein.Fig. S2. Plot ofthe number and distribution of the NOEs ... However, thesestructures do not show the coordinates ofthe atoms inFig. 6. Superposition ofthe representative NMR open conformer of the C-domain (red and blue) on the crystal structure [20] of the complex ... (C-domain). The high-reso-lution NMR structureofthe human C-domain (residues 277–437) has beendetermined in solution. The overall fold andthestructureofthe b-strandcore ofthe protein...
... amide groups of Ile128 and Arg129.Despite these differences in the coordination ofthe res-idues in the tip, the fold ofthe loop is strikingly simi-lar. The rmsd for the Ca atoms ofthe residues ... with strand b9(Fig. 1). The active site is positioned at the C-terminaledge ofthe sheet formed by the five parallel b-strands and the N-terminal ends ofthe helices a1, a7 and a9. The methionine-binding ... the tRNA that releases the loop. We speculate that the KMSKS loop could assist in the positioning of the acceptor stem in the catalytic site. The exact steering and positioning ofthe loop, and...
... ofthestructureof NADP+-bound FeRrevealed that the nicotinamide moiety of NADP+faces the re-side ofthe isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity ofthe other monomer. For the FeR dimer, the N-terminal loop ... present the crystal structure of DvMF flavoredoxin and discuss the key residues forligand binding and metal ion binding, based on the crystal structures.ResultsCloning and sequencing ofthe flavoredoxin...
... (CTF), and only data within the firstzero ofthe CTF were used. On the basis of the defocus series, this effectively limited the resolution of the reconstruction to 22 A˚. Therefore, the reconstruc-tion ... occur, and represent the inactive andthe hormone-activated states of the receptor, respectively [13,14]. It is hypothesized that ahormone-induced rearrangement ofthe ECD from the hh to the tt ... [12]. These findings havesuggested that the hh dimers, but not the tt dimers,represent the physiological structures.On the other hand, it has been proposed that the hhdimer and tt dimer structures...