site investigations and in situ testing
ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... fluorescence in situ hybridization (FISH) and microsensor techniques have a high spatial (at a single-cell resolution) and temporal resolution and great potential, and provide reliable and direct information ... nitrifiers and heterotrophs in mixed population biofilms Biotechnol Bioeng 50:24-35 Okabe, S., H Satoh, and Y Watanabe 1999a In situ analysis of nitrifying biofilms as determined by in situ hybridization ... characteristic distribution of in situ nitrifying activity was well correlated to the vertical distribution of NH4+- and NO2 oxidizing bacteria in the corresponding biofilm determined by use of FISH technique...
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prins and in situ pcr protocols
... achieved by several techniques based either on the intrinsic staining properties of the chromosomes in producing bands (the banding pattern being specific for each pair of chromosomes) (1) or ... chromosome and centromeres with biotin/PO-DAB (brown) and digoxigenin/APase-fast red (red), respectively, in a human lymphocyte metaphase spread after double-target PRINS, hematoxylin counterstaining and ... chamber, and incubate for h at 37°C in an incubator 11 Dehydrate the slides as described in step and au-dry before running the next PRINS reaction with another primer and different reporter 12 Finally,...
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Báo cáo hóa học: " A Novel Method to Fabricate Silicon Nanowire p–n Junctions by a Combination of Ion Implantation and in-situ Doping" docx
... Conclusion In conclusion, we have demonstrated a novel method to form p–n junction NW diodes by combining two well- 123 established doping techniques in- situ doping and ion implantation, in succession ... are in direct contact with the p–n junction Therefore we think that surface recombination is indeed playing a major role in the current conduction mechanism across the p–n junction resulting in ... should result in B-doped—intrinsic (p–i) type NWs, since the boron diffusion in silicon is negligible at 525°C [7], i.e., the B atoms incorporated in the lower half will not diffuse into the upper...
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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx
... However, inadequate information is available on in situ expression of both COX-1 and COX-2 within the growth plate to correlate in vitro findings with the in situ situation PGE2, the principal ... analysis and interpretation of data and was involved in drafting the manuscript CJK was involved in data interpretation, drafting the manuscript and revised it critically for the physiological and ... EP1, EP2, EP3, and EP4 in the growth plate and compared this profile with their expression in cultured growth plate chondrocytes in order to give innovative insights into in situ -in vitro correlations...
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Báo cáo y học: " Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM" pps
... determine whether cav-1 and cav-2 are in close apposition in airway epithelial cells in situ, thereby indicating an association of both proteins and formation of hetero-oligomers Distinct increase ... 20 and washed) and incubated for1 h in 5% normal goat serum containing 5% BSA in 0.005 M phosphate-buffered saline (PBS) Primary antibodies were diluted in 0.005 M phosphate buffer containing ... agonist binding [21,22] These receptors are involved in the regulation of ciliary function [23,24] In addition, several proteins involved in Ca2+-dependent signaling processes, including the Ca2+-pump...
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First principles calculations and in situ characterizations
... is cut into slices with mm in diameter, then the slice is thinned into 10 µm around by using grinding and dimpling apparatus, and finally the thin sample is post-thinned using ion milling The ... (GeO2 and Ge3 N4 ), and to study, both experimentally (using XPS and TEM) and theoretically (using first-principles calculations), the electronic, intrinsic defect, and interface properties and ... at the interface may tune band offsets Theoretically, the procedure of obtaining band offset by using first-principle calculations is actually similar to that of using XPS to determine band offsets...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 1
... consisting of mechanical consolidation, sintering and extrusion Mechanical testing however often exhibits controversial results and the understanding on deformation behavior of fine grained/nc ... behaviors Contamination during milling and grain growth during secondary process of conventional pressureless sintering are the main concerns in this synthesis process Normally contamination is more ... due to grain-boundary sliding and/ or diffusional mass transfer via grain-boundary diffusion As such, it is essential to understand and investigate the mechanical behaviors in fine grained materials...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 2
... relatively large final grain size was obtained Enhanced recovery during milling was confirmed by the corresponding low internal strain The internal strain during milling showed inverse grain size dependence ... the reinforcementmatrix interfaces are clean, resulting in strong interfacial bonding; 35 Literature Review in- situ formed reinforcing particles are finer in size and their distribution in the ... vacuum or in a reducing medium such as toluene and performing powder handling in an argon-filled glove box can minimize the atmospheric contamination [117] The reduction in grain size in powder...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 3
... was observed in all MMed-1250 samples 53 In- situ formation of Aluminium Nitride composite powder Al and Fe contamination from milling media formed FeAl2 intermetallic during milling Compared ... shown in Figs 3.1(a) and (b) Due to the high concentration of nitrogen in parazine, relatively fast direct nitriding reaction compared to NH3 gas can be maintained during the milling process During ... then incorporated in the interfaces by the pressure welding of the Al powder followed by diffusing into the Al matrices through grain boundaries, dislocations and other defects [25] Milling induced...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 4
... strengthening from grain refinement after 10h-MM is more pronounced for pure Mg indicating grain refinement strengthening mechanism dominates in the initial milling of 10h and 20h in the composite ... of milling indicating no apparent influence of second phases and reinforcement on melting with longer milling duration It is interesting to note that milling has no influence on the melting of ... reduction in grain size and introduction of microstrain during milling In Fig 4.1(b), intensities of Mg (100) and (110) peaks for the extruded specimens increase with milling duration, confirming the...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 5
... strain rates as a function of milling duration are illustrated in Fig 5.4 Generally, gradual increase in ductility was observed with decreasing strain rate for all milling durations and increasing ... ductility is observed with decreasing strain rate and increasing milling duration However, no significant increase in ductility with milling duration at the highest strain Elongation (%) rate is detected ... increasing milling duration at all strain rates It is quite interesting that the loading curves for 0h-MMed samples at all strain rates are almost identical and the slight increase in ductility...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 6
... constituents, since the Mg grain size is in nanoscale and thus less likely for the reinforcement and in- situ formed particles to exist in the interior of the Mg grain, this effect should be negligible in ... grain boundaries Energy dissipated in both the grain-boundary sliding and interfacial reaction is negligibly small for conventional grain sizes since grain boundaries will act as perfect 143 Investigation ... logarithmic coordinates of and is shown in Fig 6.1 The relationship between stress and creep strain rate is not linear at different temperatures Two outstanding features of the influence of particles...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 7
... samples This finding implies that the grain refinement to a certain length scale diminishes the significance of solid solution strengthening and dispersion hardening 30hMMed composite exhibited ... grain sizes and then performing systematic tests under identical conditions of temperature and stress for the purpose of determining the values of the grain size sensitivity, and (c) performing ... reinforcements (insitu formed second phase particles and/ or ex -situ reinforcement particles) when the grain size becomes very small in nanometer range Grain boundary activities played an important role in...
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A systematic approach for preferential crystallization thermodynamics, kinetics, optimal operation and in situ monitoring
... differential scanning calorimetry (DSC) 69 Table 4.4 Melting points and enthalpies of fusion of (+)-N-methylephedrine and (±) N-methylephedrine; entropy of mixing of (+)- and (-)-Nmethylephedrine in the ... help I am greatly indebted to Chemical and Process Engineering Centre (CPEC, NUS) and Division of Chemical and Biomolecular Engineering, NTU, for providing research facilities Finally, this thesis ... balance modelling (Randolph and Larson, 1988) The newly developed on-line monitoring and controlling techniques provide good tools to facilitate this kind of investigation (Barrett and Glennon,...
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A study on manganese catalyzed epoxidation of styrene using online raman and in situ FTIR monitoring techniques
... how interesting and fulfilling the actual experience can be Research can be utterly tedious and involves unassuming patience cum perseverance working towards making scientific contribution in ... the reaction mechanism and carry out kinetic studies using online Raman, in- situ FTIR, 13C NMR and UV-Vis spectroscopy coupled with application of Band Target Entropy Minimization (BTEM) chemometric ... (a) Chlorinated Mn-porphyrin complex, (b) Mn-porphyrin mediated epoxidation using imidazole and (c) N-alkyl imidazole and benzoic acid Figure 2-7 (a) Iron porphyrin and (b) Iron porphyrin mediated...
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Experimental investigations and CFD study of temperature distribution during oscillating combustion in a crucible furnace
... Engineering from Institution of Engineers (India) in 1991 and Master’s degree from Delhi University in 1996 His area of research is Oscillating Combustion He developed an oscillating valve and incorporated ... Narsaiah, working as Assistant Professor in PRRMEC, Shabad He has done his M.Tech in Industrial Engineering and Management from JBIET ( JNTU Hyderabad) in 2009 and B.Tech., in Mechanical Engineering from ... numerical results and also the magnitude of temperatures obtained during oscillating combustion The T1 temperature point for experimental and simulation predictions are in quite convincing and the deviations...
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Tài liệu Leading the way in IT Testing and Certification Tools pptx
... reinstalling the operating system, or as in proposed in this scenario, by repairing the installation Note: Leading the way in IT testing and certification tools, www.testking.com - 27 - During ... Explanation: Windows XP includes an uninstall feature which allows us to uninstall Windows XP and return to the previous operating system Incorrect Answers: Leading the way in IT testing and certification ... Leading the way in IT testing and certification tools, www.testking.com - 40 - You are the senior network administrator at TestKing.com You instruct your trainee to install and configure Windows...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... weak, binding of ACP to the docking site followed by a conformational change in ACP proposed to inject the acyl chain into the substrate-binding pocket [32] If a similar mechanism is involved in ... octahedrally co-ordinated in the vicinity of the active site (Fig 2) Three of the six cation ligands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387 The ... Histidines and lysine in KAS I ⁄ KAS II catalysis P von Wettstein-Knowles et al Scheme group because of the cysteine and two histidine activesite residues [1–3] Another group of decarboxylating...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
... reported in four siblings, who, though exhibiting a biochemical profile indicative of MCAD deficiency (i.e elevated hexanoylglycine in urine), have so far remained clinically and developmentally ... added during the purification, and certainly does not indicate the level of contamination by other protein, to judge from SDS ⁄ PAGE The ratios for R256T (8.8) and K364R (9.6), both handled in exactly ... prevents catalysis Indeed this residue has recently been studied in rat MCAD, where the arginine was mutated to alanine, lysine, glutamine and glutamic acid The authors found that the lysine mutant exhibited...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... proteins into the ER [18] The strong inhibition of the processing of pro-cathepsin E into the mature enzyme by brefeldin A indicates that the processing and maturation of this protein occur in ... propeptides, and N-glycosylation of cathepsin E in its processing, maturation, and trafficking must be of particular importance In this study, to understand the molecular basis of the processing and intracellular ... pro-cathepsin E in the culture Recombinant cathepsin E and its mutants medium (Fig 2C) Given that bafilomycin A1 effectively inhibits acidification of intracellular acidic organelles including endosomes,...
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