site and function of protein synthesis
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... interaction between v-KIND and MAP2, as well as its biological significance, remains unclear In the present study, we determined the structural and functional properties of the protein protein interaction...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc
Ngày tải lên :
07/03/2014, 00:20
... identification and distribution of TLP genes in nature, the structural and functional characterization of the TLP from various organisms, and the evolution of TLP and transthyretin The identification of TLPs ... observed in b-strands A, G and H [17] Structural nature of the TLP and transthyretin active sites One of the striking features of transthyretin is the central channel of the protein into which ... S dublin TLP and zebrafish TLP are found in the flexible portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17] Greater...
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Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx
Ngày tải lên :
07/03/2014, 01:20
... activity of RP mRNA for adjustment of the biosynthesis of ribosomes to the requirements of cell growth and differentiation In addition, a relevant contribution of protein turnover to the regulation of ... the study of the synthesis and function of RPs has both expanded our knowledge FEBS Journal 276 (2009) 3199–3210 ª 2009 The Authors Journal compilation ª 2009 FEBS 3205 Ribosomal protein synthesis ... ribosome pathologies have had in our understanding of regulatory mechanisms of RP synthesis Translational regulation of RP synthesis Sequence comparison of some vertebrate RP genes cloned in the...
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Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identification of two active site cysteines pptx
Ngày tải lên :
08/03/2014, 10:20
... were used in the presence of 10 mM ATP For determination of the Km of ATP, different concentrations of ATP were used in the presence of mM GlcNAc Site directed mutagenesis Site directed mutagenesis ... phosphoenolpyruvate, 2.5 U pyruvate kinase and variable amounts of protein extracts Incubations were carried out at 37 °C for 30 min, and reactions were stopped by addition of 350 lL of ethanol Radiolabeled compounds ... Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur J Biochem 269) 4213 Fig De novo and salvage pathway of UDP-GlcNAc biosynthesis EXPERIMENTAL PROCEDURES Materials...
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Báo cáo y học: " Differential expression and function of breast regression protein 39 (BRP-39) in murine models of subacute cigarette smoke exposure and allergic airway inflammation" pps
Ngày tải lên :
12/08/2014, 13:22
... between BRP-39 KO mice and WT controls in terms of the BAL inflammatory profile (Figure 6B) We observed similar numbers of mononuclear cells and eosinophils in the BAL of BRP-39 and WT mice Flow cytometric ... cytometry pseudo-dot plots of data generated from for the lung digests of day smoke exposed lungs (A,C,D) and smoke- and OVA-exposed mice after month of cessation and days of rechallenge with OVA ... molecule YKL-40 and the murine homologue breast regression protein (BRP)-39 are upregulated in a variety of inflammatory conditions [12-14] Two members of this family of enzymatically active and inactive...
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Báo cáo y học: "Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura" ppsx
Ngày tải lên :
14/08/2014, 14:21
... Predicted and aligned binding sites in pCRMs Predicted and aligned binding sites in pCRMs Predicted binding sites and aligned binding sites (see Materials and methods) in positive, ambiguous and negative ... individual functional binding sites and the overall composition of functional CRMs will be conserved [25,26] Conservation of binding -site clustering is a specific evolutionary signature of this class of ... examined our functional (positive) and non-functional (negative) pCRMs and noticed that in the positives, the lower bound for the number of conserved sites as a function of D melanogaster sites followed...
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Báo cáo y học: "Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the protein" ppsx
Ngày tải lên :
14/08/2014, 14:21
... distributions of mouse proteins (a) Expression pattern of all mapped proteins expressed in at least a single tissue (5,528 out of 6,242 proteins) (b) Expression pattern of proteins in different functional ... Number of tissues in which protein is expressed Figure Expression distribution of mouse proteins functional groups Expression distribution of mouse protein functional groups (a) The metabolic functional ... phyletic age of a protein and its expression profile, and related this to the function of the protein The term 'phyletic age' used here describes an estimated point in time when a protein integrated...
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Báo cáo y học: "Creation and disruption of protein features by alternative splicing a novel mechanism to modulate function" docx
Ngày tải lên :
14/08/2014, 14:21
... forms protein with functional unit protein without functional unit protein without functional unit protein with functional unit (b) gene two splice forms Figure mechanisms to alter linear protein ... sequence The disruption of the unit results in a loss of function The shorter splice form encodes a protein that puts together both parts of the unit which results in a gain of function (complete ... between exon and Disruption of the single TM domain, soluble protein DPP8 Dipeptidylpeptidase NM_017743 NM_197961 Exon between exon 15 and 16 Disruption of the single TM domain, soluble protein COX7A2...
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EXPRESSION AND FUNCTION OF THE PRL FAMILY OF PROTEIN TYROSINE PHOSPHATASE
Ngày tải lên :
24/08/2014, 11:44
... December 2012 Expression and Function of the PRL Family of Protein Tyrosine Phosphatase Major Professor: Stephen K Randall The PRL family of enzymes constitutes a unique class of protein tyrosine phosphatase, ... transcription factor function The combined results of these studies have expanded our current understanding of the expression and function of the PRL family of enzymes as well as of the role these ... Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Expression and Function of the PRL Family of Protein Tyrosine Phosphatase For the degree of Doctor of Philosophy Choose...
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Characterization and function of two g protein regulators, vertebrate LGN and drosophila RapGAP
Ngày tải lên :
12/09/2015, 09:42
... heterotrimeric G-proteins comprising of Gα, Gβ and Gγ subunit In the absence of ligand-mediated activation, the Gβγ dimer is tightly bound to Gα-GDP and intracellular domain of GPCR The binding of an extracellular ... class of proteins include Partner of Inscuteable (Pins) in Drosophila, LGN and AGS3 in vertebrates and GPR1 and GPR2 in C elegans This important class of GoLoco-motif containing G- Introduction protein ... comprising of Baz and DaPKC and the other comprising of Pins and Gαi The localized activity of either pathway alone is sufficient to mediate the generation of an asymmetric mitotic spindle and unequal...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
Ngày tải lên :
14/02/2014, 18:20
... implications of the cycle of histone acetylation and deacetylation that accompanies cycles of transcription, and highlight the special significance of histone H4 lysine 16 acetylation Active chromatin and ... SCS and SCS’ elements are the prototypes of one of the major classes of BE in Drosophila, which bind a protein complex termed BEAF [77] This complex is associated with about half of the interbands ... eviction of two positioned nucleosomes that otherwise occupy two discrete sets of factor binding sites and block binding of the constitutively expressed factors Sp1 and Runx1 Upon activation, NFAT and...
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Tài liệu Body Size: The Structure and Function of Aquatic Ecosystems pptx
Ngày tải lên :
17/02/2014, 19:20
... patterns and processes at different levels of biological organization: from the life histories of individuals, to the structure and dynamics of populations and communities, to the fluxes and pools of ... variety of ecological phenomena, taxonomic and functional groups of organisms, and types of ecosystems Here we apply the metabolic theory of ecology to focus on some important correlates and consequences ... measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems The total biomass per unit area, W, is simply the sum of the body mass of all...
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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
Ngày tải lên :
19/02/2014, 06:20
... occur in a variety of proteins involved in cell signaling, membrane trafficking, and polarity establishment, and function as phosphoinositide-binding modules in the assembly of proteins at membrane ... expression of alternatively spliced transcripts of the NOXO1 gene, by PCR using variant-specific primers, and the roles of the protein products in activation of Nox oxidases Results and Discussion ... 2006 FEBS 3665 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and Noxo1c in various human tissues To compare the distribution pattern of Noxo1b and Noxo1c, we designed variant-specific...
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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt
Ngày tải lên :
19/02/2014, 06:20
... extent of apoptosis but also the ability of TRAIL to regulate the initiation of translation at the level of eIF2a phosphorylation and 4E-BP1 dephosphorylation Control of protein synthesis by IFNa and ... indicate the concentrations of TRAIL producing 50% inhibition of protein synthesis 3700 Fig Effects of zIETD.FMK on TRAIL-induced inhibition of protein synthesis and caspase-8 activity in MCF-7 ... the inhibition of protein synthesis by TRAIL by 80% (Fig 2A) and, conversely, IFNa enhanced the sensitivity of protein synthesis to TRAIL in MCF-7 cells and HeLa cells by 2.5-fold and 10-fold,...
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Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx
Ngày tải lên :
19/02/2014, 12:20
... isoforms by the excision of the prosegment and of most of the PSI [21] Conversely to what has been found in vivo [31], heavy and light chains of the processed forms of recombinant cyprosin are ... The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of the mature enzyme sequence and the catalytic ... protein, gliadin Localization of both proteins in the wheat seed endosperm also favored this hypothesis [48] The involvement of plant APs in proteolytic processing and maturation of storage proteins...
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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
Ngày tải lên :
19/02/2014, 12:20
... domain of each subunit contributes to the formation of the hydrophilic pore ACh binding protein has structural and functional homology to the extracellular ligand binding domain of the nAChR, and ... analysis of the DNA of different Conus species has already revealed a large number of a-conotoxin sequences [45] and the identification of further specific nAChR ligands is likely The advantage of a ... in the formation of native MII binding sites, but may reflect the Vc1.1 scarcity of these sites in the investigated brain tissues and/ or the formation of low affinity binding sites that are not...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
Ngày tải lên :
19/02/2014, 16:20
... size of the coils and the helical handedness are different, with the spruce budworm protein consisting of 15-residue coils with a left-handed fold and the Tenebrio molitor protein consisting of ... AFP isoforms In addition to in vitro mutations, the comparison of isoform sequences can demonstrate which residues are important for a protein s function and structure A list of known isoforms ... structure and PDB code shown below each panel (A) Overlap of isoform 337 with the structure of the longer isoform CfAFP-501 using the main chain of residues Thr23fiAsn90 in isoform 337 and residues...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
Ngày tải lên :
06/03/2014, 09:22
... SPE and Easter [31] Injection of either of these M sexta proteinases into larvae stimulated the expression of antimicrobial peptide genes, suggesting that they might function in activation of ... plasma proteins that perform immune functions This mechanism leads to activation of phenoloxidase, which oxidizes catechols, leading to the formation of toxic quinones and melanin [1,2], and to ... An et al Manduca sexta Spatzle ¨ Introduction A prominent feature of the innate immune systems of insects is the activation of serine proteinase cascade pathways in hemolymph, which function to...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
Ngày tải lên :
06/03/2014, 11:20
... of eRF1 Fig S11 The Ramachandran map plot (/ and w torsion angles for the protein backbone) of all 24 conformers of the NMR families of solution structures of the closed and open conformers of ... minidomain and the zinc-binding domain of the zinc finger protein Ynr046w [25] (Fig 8) The fit of the heavy atoms (Ca, C, and N) from three b-strands and the a-helices of both the closed and the open ... [19] Four b-strands (b1, 301–303; b2, 320–323; b6, 389–392; and b7, 409–412) form a b-sheet with three antiparallel strands (1, and 7) and strand 6, which is parallel to strand b-Strands are located...
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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
Ngày tải lên :
07/03/2014, 05:20
... prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Protein Eng 10, 1–6 13 Song J, Quan H & Wang C (1997) Dependence of the anti-chaperone activity of protein disulphide ... integrated elution peak areas, and the PDI’s oxidase and isomerase activities were expressed as the initial rate of decrease of linear tx3a and the initial rate of increase of nTx3.1, respectively To ... of disulfide oxidase and isomerase based on the model of hirudin folding Anal Biochem 339, 94–103 Walker KW, Lyles MM & Gilbert HF (1996) Catalysis of oxidative protein folding by mutants of protein...
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