signaling regulation and specificity in the growth hormone prolactin system of horm
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
Ngày tải lên :
19/02/2014, 05:20
... probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the p53 sequence-specific DNA binding to these ... DNA cis-platination on sequencespecific DNA binding of p73 proteins We tested the in uence of DNA modification with cisplatin on the binding of two p73 isoforms, p73d and p73b, to the p21 target ... 5) The intensity of the resulting R73d band decreased almost linearly with increase in the cis-platination level; for rb ¼ 0.06, about 90% inhibition of p73d–p21 binding was observed Almost the...
- 14
- 597
- 0
Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
Ngày tải lên :
21/02/2014, 03:20
... reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasons to explain the differences in the mechanism of internalization ... mechanism of cellular internalization of the peptide thus appears unlikely The number of arginine residues within the Tat peptide appeared to be the main determinant for maintaining a high translocating ... peptide nine arginine residues in length The D-form and the retro-inverso form of the polyarginine peptide were found to internalize more ef®ciently However the higher stability in serum containing...
- 8
- 485
- 0
Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
Ngày tải lên :
07/03/2014, 21:20
... effector domain; MD, middle domain; PH, pleckstrin homology; PRD, proline- and arginine-rich domain dynamins, (b) the structural and physicochemical properties of DRPs and (c) DRPs engaged in the division ... domains of effector proteins of the actin cytoskeleton Dynamins are required in phagocytosis and in caveolae- and clathrin-dependent endocytosis [71] Of the three conventional mammalian dynamins, ... in DRPs Examples of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins are interferon-inducible DRPs [98] Like dynamin, they self-assemble and bind and...
- 13
- 372
- 0
Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
Ngày tải lên :
16/03/2014, 14:20
... assays Following the assay, we recovered a fraction of the protein in the pellet (lane 1, Fig 1B) and the remainder in the supernatant (lane 5) In the presence of ssDNA, the protein fraction that ... described in this study help us to understand the transitions associated with the oligomeric states of hRad51 and hRad52 proteins in the presence of ssDNA and relate them to their DNA binding 204 ... describe the changes associated with the aggregation states of hRad51 and hRad52 in the presence of 121-mer ssDNA and hRad52 mediated assembly of the ssDNA–hRad51 complex The changes in the protein...
- 9
- 378
- 0
Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
Ngày tải lên :
23/03/2014, 03:20
... peptide mass fingerprints allowed the identification of the corresponding proteins in the database (Table 2) Database search and functional exploration of these proteins revealed that they were associated ... pointing to a reduction in NADPH available for fatty acid biosynthesis Taken together, these results seem to be indicative of an adaptation in favor of liver gluconeogenesis in response to the ... acid metabolism The reduction in d-amino acid oxidase expression in the kidney proteome is interesting, in view of the contribution of this enzyme to glyoxylate production from glycine Thus, it...
- 9
- 481
- 0
Báo cáo khoa học: Biogenesis of peroxisomes Topogenesis of the peroxisomal membrane and matrix proteins ppt
Ngày tải lên :
23/03/2014, 13:20
... N-terminus of the receptor [62,63] The current understanding of the organization of the peroxisomal import machinery for PTS1 proteins is summarized in Fig In the absence of cargo protein, Pex5p ... peroxisomes Again, how these proteins reach the ER and their final destination in the peroxisomal membrane is unknown post-translationally into the ER independent of the sec-machinery [112] The mechanisms ... membrane proteins insert into the membrane remains to be investigated As outlined above, the topogenesis of Pex3p seems to be different from that of other PMPs The N-terminal 50 amino acids of Pex3p...
- 11
- 556
- 0
applications of chimeric genes and hybrid proteins, part a
Ngày tải lên :
10/04/2014, 11:02
... translational unit The deletion covered the sequences coding for the carboxy terminus of the rlIA protein and for approximately 10% of the amino terminus of the rlIB protein Despite the absence of a substantial ... aid in the study of protein folding Luzzago and Cesareni 24 used a cute fusion approach to isolate mutants affecting the folding of ferritin Other such ideas must be waiting in the wings The ... and the bands of interest are cut from the gel and purified The specifics of this part of the procedure are not relevant in this instance Suffice it to say that the bands are cut, purified, and...
- 614
- 282
- 0
applications of chimeric genes and hybrid proteins, part b
Ngày tải lên :
10/04/2014, 11:02
... depends on the affinity of the ligand-receptor interaction For the interaction of receptor tyrosine kinases with their cognate ligands, the dissociation constant of binding, Ko, is generally in the ... staining is seen in several areas, including the dorsal spinal cord, limb buds, somites, and branchial arches, and most strongly in the developing midbrain, which is seen here at the top of the ... After the addition of peptide, the eluted tagged protein band will appear again in the eluate The elution in which the strongest band appears will have the greatest concentration of eluted protein...
- 696
- 393
- 0
báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
Ngày tải lên :
18/06/2014, 15:20
... serine-threonine kinases and other signaling proteins in the absence of PPAC, would therefore enhance phosphorylation of substrate proteins and maintain a downregulated state of VEGFR kinase in ... provided new insights into the tyrosine kinasesignaling pathways likely to be generated by numerous PTKs, serine threonine kinases and other signaling proteins identified in the present study These ... the RGD domains are present in numerous integral plasma membrane proteins identified in this study including integrin and other cell adhesion proteins [82] In addition, the binding of Tat to VEGFR...
- 24
- 507
- 0
báo cáo hóa học:" Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma" docx
Ngày tải lên :
18/06/2014, 15:20
... 0.05 for each protein) Results Increase in the expression of p53, p21/WAF1, and MDM2 in HCC The expression of p53 and MDM2 in the pairs was higher in the HCC tissues than in the adjacent hepatic ... progression and decreasing cell growth MDM2 is the product of a p53-inducible gene and inhibits the p53 activity by ubiquitinating p53 and creating a negative-feedback loop [58] Altered expression of these ... in the nuclei of the cancer cells and highly expressed in the HCC Figure Expression of p53, p21/WAF1 and MDM2 in HCC by IHC Expression of p53, p21/WAF1 and MDM2 in HCC by IHC The hematoxylin and...
- 8
- 611
- 0
báo cáo hóa học: " Leader (L) and L* proteins of Theiler''''s murine encephalomyelitis virus (TMEV) and their regulation of the virus'''' biological activities" docx
Ngày tải lên :
19/06/2014, 22:20
... [30,31], and the second is cleavage of the p220 component of the cap-binding protein complex [32], resulting in the shut off of host protein synthesis On the other hand, the function of L of the cardioviruses ... demyelinating disease 1–2 months p.i Clinical signs include spastic paralysis, inactivity and urinary incontinence The demyelination mainly affects the spinal cord, with an unexplained sparing of the ... matter These pathological findings are reminiscent of MS, that is, inflammatory cell infiltration and loss of myelin in the face of relative preservation of axons [1-4] Therefore, this demyelinating...
- 8
- 447
- 0
Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... TLR signaling, but instead regulate paracrine IFN signaling [7] The SOCS protein family is comprised of eight proteins (CIS, cytokine-inducible SH2-containing protein, SOCS17) of structural and ... This finding is in keeping with the findings of NS1/NS2 antagonism of type I IFNs [4,46,47] and suggests the possibility that type I IFN antagonism is linked to NS1/NS2 induction of SOCS1 and subsequent ... [29], and recent RNA interference (RNAi) studies in mice targeting NS proteins for silencing by short interfering RNA (siRNA) resulted in inhibition of RSV replication in mice [67] The NS1 and...
- 11
- 435
- 0
Báo cáo y học: "Diagnostic value and clinical laboratory associations of antibodies against recombinant ribosomal P0, P1 and P2 proteins and their native heterocomplex in a Caucasian cohort with systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 15:22
... contains all immunological domains of the subunits P0, P1 A) and P2, there were considerable differences in the cutoffs and in sensitivities for the detection of aRibP N H, aRibPR0, aRibPR1 and ... detectable in the plasma membranes of different cells, including lymphocytes [11] Further, the aRibPs are able to bind and penetrate T-cell lines [44,45], and especially aRibP R can induce apoptosis in ... for the integrity of the data and the accuracy of the data analysis RB, CD, FH and WSchlumberger contributed to study design FB, RB, US, KE and AR contributed to the acquisition of data FB, RB and...
- 11
- 415
- 0
Báo cáo y học: "Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element" doc
Ngày tải lên :
13/08/2014, 05:21
... optimize the integrity of the fractions, nuclear and cytoplasmic RNAs were obtained, and samples were subjected to Northern blotting and staining to confirm the isolation of intact RNA and absence of ... Crm1 dictates Rem protein export independent of the vector RNA Nevertheless, Rem binding to the 3' RmRE, perhaps in the cytoplasm or after binding of a cellular protein in the cytoplasm, may promote ... Rev and Rex also use Crm1 for the export of introncontaining homologous RNAs [32, 41] To test the involvement of Crm1 in human cells, we tested whether a dominant-negative nucleoporin involved in...
- 13
- 246
- 0
Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
Ngày tải lên :
13/08/2014, 09:21
... Figure of loaded onto the gel Relation4 the intensities of the bands in the lanes with recombinant PFV proteins shown in Fig and amounts of protein Relation of the intensities of the bands in the ... of the determination of the relative amounts of Gag and Pol proteins in purified PFV Representative example of the determination of the relative amounts of Gag and Pol proteins in purified PFV ... Gag and Pol proteins were also applied to the gel The intensities of the bands were determined with a LAS-3000 (Fujifilm) and the relative amounts of Gag and Pol proteins were calculated using the...
- 7
- 318
- 0
Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
Ngày tải lên :
14/08/2014, 16:21
... that the protein has additional functions in the cell The kinesins present at kinetochores in H sapiens are CENP-E (Kinesin-7) and MCAK (Kinesin13), and in S cerevisiae Kip3 (Kinesin-8), Cin8 (Kinesin-5), ... cerevisiae are Kip3 (Kinesin-8), Cin8 (Kinesin-5), Kip1 (Kinesin-5) and Kar3 (Kinesin-14), while in S pombe they are Klp5 (Kinesin-8), Klp6 (Kinesin-8) and Klp2 (Kinesin-14) (for nomenclature ... in ≥ 80% of the organisms and black letters on green, similar residues in ≥ 80% of the organisms Schematic drawings above the alignments indicate the length of the S cerevisiae proteins and the...
- 21
- 384
- 0
Studies on interactions between HCRSV coat protein and host proteins from kenaf
Ngày tải lên :
11/09/2015, 10:17
... range of interactions through the electrolyte Protein-protein interactions are at the core of the entire interactomics system of any living cell And the interaction between the viral proteins and ... 1.3 Protein-protein interaction study Protein-protein interactions are the critical part in the process of the virus-host interaction It involves not only the direct binding of protein molecules ... protein, a putative chaperon P13.9, a C2 domain-containing protein, a ricin domain-containing protein and putative alpha-D-xylosidase like protein The interaction of CP and SO was confirmed by in...
- 190
- 380
- 0
X ray crystallographic study of yeast dcp1 and dcp2 proteins insights into the mechanism and regulation of eukaryotic mRNA decapping
Ngày tải lên :
14/09/2015, 12:28
... machinery The proteins involved in the mRNA 5’ decay pathway aggregate to form the 5’ decay machinery, including the factors in RNA binding, RNA remodeling, complex scaffolding and catalysis The ... regulation, interacting with the actin binding protein WASP interacting protein, WIP A minimal 25 amino acids peptide containing the DLPPPEP motif found in WIP is required for interaction with WASP The ... has been retained with the Dcp2 protein on gel The deletion of the C-terminal region, containing Box B, in the hDcp2 protein abolishes the RNA binding capacity of protein truncated So the Box B...
- 125
- 343
- 0
Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins
Ngày tải lên :
04/10/2015, 10:24
... this tyrosine with the SH2 domain, which leads to the binding of the SH3 domain to the linker region connecting the SH2 domain and the tyrosine kinase domain However, the question is how tightly ... with the ATP binding site In contrast to Src, mutation of Tyr-447 to Phe in Brk results in decreasing the ability of Brk to induce anchorage-independent growth Localization of the Brk tyrosine kinase, ... Without the understanding of the structures of the involved proteins, the whole map of all pathways related to peroxins will not be complete Interestingly, the interaction between Pex8, Pex20 and...
- 74
- 255
- 0