semaphorins and their receptors in vertebrates and invertebrates
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
Ngày tải lên :
26/10/2012, 09:07
... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... uptake from the circulating arginine and (b) recycling of citrulline to argi- 152 nine by the actions of AS and AL L-arginine is transported into synaptosomes (46), neurons (47) and astroglia (48)...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
Ngày tải lên :
07/03/2014, 12:20
... element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from different brain regions contain different ... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus ... few minutes later leads to an irreversible and sustained increase in intracellular [Ca2+]c in apical dendrites [151] DCD is invariably demonstrated in every neuronal cell type studied, i.e spinal...
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Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc
Ngày tải lên :
14/03/2014, 23:20
... orthologs in other plant species, including MPK3 and MPK6 in Arabidopsis (group A MAPKs), SIMK and SAMK in alfalfa, and LeMPK1 ⁄ (Lycopersicon esculentum SA-induced protein kinase) and LeMPK3 ... transduction [9] In tobacco, salicylic acid (SA)-induced protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation with the tobacco mosaic virus (TMV) [10] Their ... accumulation in wild-type and transgenic plants in response to abiotic and abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by DAB staining (B) Abiotic stress-induced H2O2 and OÀ...
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Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
Ngày tải lên :
16/03/2014, 14:20
... peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumor suppression Nature 424, 561–565 60 Kinnula VL, Paakko P & Soini Y (2004) Antioxidant ¨ ¨ enzymes and redox regulating thiol proteins in ... phase, treated during 40 with no agent as a control or with 1.2 mM H2O2 and 0.1 lgÆmL)1 antimycin A (anti A), singly or in association, as indicated in the figure The symbols –, + or ± in the last column ... H2O2, 0.1 lgÆmL)1 antimycin A (anti A), and 2.5 lgÆmL)1 FCCP singly or in association Proceedings were performed as described in Fig detectable expression in both wild-type and tsa1D cells: TSA2,...
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
Ngày tải lên :
17/03/2014, 03:20
... calcium binding sites in annexins is conserved in domain I only and is present in a modified form in the fourth domain Despite extensive efforts, we have not yet been successful in obtaining a calcium-bound ... occluding access to the binding site A bidentate ligand required for canonical calcium binding is also missing Membrane binding loops For reasons of homology, it is likely that the AB and DE ... calcium binding in the loops of domain I might be possible in Anx(Gh1), in contrast to the bell pepper annexin The protein contains a highly unusual sulfur cluster formed by two adjacent cysteine...
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Báo cáo khoa học: Photodynamic treatment and H2O2-induced oxidative stress result in different patterns of cellular protein oxidation ppt
Ngày tải lên :
23/03/2014, 15:21
... are shown in (A) and (B) Silver staining is shown in (C) and (D) in blue superimposed with the spots of oxidized proteins shown in red Protein labels: 1, a-tubulin; 2, b-tubulin; 3, actin; 4, PDI; ... tyrosine residues in a target protein In the first experiments, we assessed whether PDT can cause dityrosine (dityramine) formation, and the covalent coupling of the Tyr-Fluo to a model protein ... acetylTyr-Fluo, resulted in the labeling of cellular proteins, as shown in Fig PDT-induced protein labeling was dependent on the concentration of the photosensitizer (Fig 3, lanes and 4) and the dose of...
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báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx
Ngày tải lên :
19/06/2014, 22:20
... were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet (2 ... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... by examining whether administration of indomethacin in combination with vitamin E would result in a better anti-amyloidotic effect Our findings show that soluble Aβ1-40 and Aβ1-42 levels were...
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Báo cáo y học: "Nifedipine decreases sVCAM-1 concentrations and oxidative stress in systemic sclerosis but does not affect the concentrations of vascular endothelial growth factor or its soluble receptor" potx
Ngày tải lên :
09/08/2014, 01:23
... nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1 Endothelium injury is critical in SSc and is suspected ... factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative stress and angiogenesis is emphasised in SSc by the baseline correlation ... (carbonyl residues and advanced oxidation protein products [AOPP]) at baseline and, secondly, to look for the influence of nifedipine treatment on all these parameters in SSc patients mined by Doppler...
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Báo cáo y học: " Impaired glucose transporter-1 degradation and increased glucose transport and oxidative stress in response to high glucose in chondrocytes from osteoarthritic versus normal human cartilag" pps
Ngày tải lên :
09/08/2014, 14:21
... by local inflammation and changes in the subchondral bone Increasing age, excessive loading or injury, genetic predisposition and obesity are important risk factors for the development and progression ... concentration is identical to and reflects that in the plasma, both under normal conditions and in noninflammatory and inflammatory types of arthritis, excluding those associated with infections [12] To ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...
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Báo cáo khoa hoc:" Lack of association between mutations of gene-encoding mitochondrial D310 (displacement loop) mononucleotide repeat and oxidative stress in chronic dialysis patients in Taiwan" ppsx
Ngày tải lên :
11/08/2014, 07:21
... oxidative damage, we intend to further investigate the possible link between oxidative stress and mtDNA mutations mtDNA deletions/insertions and base changes mainly involve purine transitions, which ... forward and reverse primers in a final volume of 20 μl PCR reactions were conducted as follows: initially at 50°C for min, 95°C for min, 40 cycles for denaturation at 95°C for 15 s, annealing at ... biomarkers in the blood and in tissue DNA In our previous study, we demonstrated that, in continuous ambulatory peritoneal dialysis (CAPD) patients, the level of oxidative stress biomarkers in the...
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Báo cáo y học: "Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinscher" potx
Ngày tải lên :
13/08/2014, 13:20
... with copper incorporated during biosynthesis Metallothionein 1A (MT1A) is a small intracellular protein capable of chelating several metal ions, including copper It contains many cysteine residues, ... dehydrated and embedded in paraffin Sections (4 µm thick) were stained with haematoxylin-eosin, van Gieson's stain, reticulin stain (according to Gordon and Sweet), and with rubeanic acid One experienced ... cinnamon (LEC) rats during the development of hepatitis Eur J Clin Invest 1998, 28:302-310 Daffada AA, Young AP: Coordinated regulation of ceruloplasmin and metallothionein MRNA by interleukin-1...
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Báo cáo y học: "Boswellic acids extract attenuates pulmonary fibrosis induced by bleomycin and oxidative stress from gamma irradiation in rats" doc
Ngày tải lên :
13/08/2014, 14:20
... determined according to Sinha [58] Histological examination The lungs were removed, inflated at 25 cm pressure with PBS then fixed, embedded in paraffin, sectioned and stained with hematoxylin and ... inflammatory responses following BL administration were attenuated by BA administration An increased eosinophil number in BALF was reported following intratracheal BL administration, suggesting ... cancer and can cause pulmonary fibrosis as a complication, has been used in research to induce pulmonary fibrosis in mice and rats and produce an oxidative injury resulting in DNA damage and the...
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Báo cáo y học: "Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats" potx
Ngày tải lên :
13/08/2014, 21:21
... hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation Clin Sci (Lond) 2008, 115:91-97 31 Maines MD, Mayer RD, Ewing JF, McCoubrey WK Jr: Induction of ... NaHS on inflammatory pathway signaling (a, d) Western blots revealing NF-kB expression in the aorta (a) and in the heart (d) (b, e) Western blots revealing P-IB expression in aorta (b) and in heart ... (baseline) and every 10 minutes during the observation period In vitro measurements Determination by electron paramagnetic resonance (EPR) NO spin trapping Aorta and heart samples were incubated...
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Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt
Ngày tải lên :
14/02/2014, 12:20
... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNAbinding domain (Sakai et al 1998) called a GARP domain (Riechmann et al 2000) and the proline- and glutamine-rich ... harpin-induced 1) was increased in tobacco leaves On the other hand, spermine induces mitochondrial dysfunction and activation of SA-induced protein kinase (SIPK) and wound-induced protein kinase ... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al 2005) In other plant species, it was also shown that mutations in BRI1...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
Ngày tải lên :
17/03/2014, 03:20
... for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 mM) to determine ... provided against Na/K-ATPase inactivation was not due to the removal of fructose by binding to a-crystallin, BSA was resealed (in the same manner and concentration as that of a-crystallin) and incubated ... with lysine residues, whereas methylglyoxal reacts principally with arginine residues although modification of lysine and cysteine also occurs [2,28] H2O2 oxidizes methionine and cysteine residues...
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báo cáo khoa học: " Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii" ppsx
Ngày tải lên :
11/08/2014, 11:21
... few and weak phenotypic changes (Figure 5C) A general increase in antioxidant levels including zeaxanthin, antheraxanthin, lutein and a-tocopherol and a significant rise in NPQ was detected in ... under HL conditions, and this correlated with increased lutein and zeaxanthin levels in this mutant Despite the important role of zeaxanthin and antheraxanthin in photoprotection and prevention of ... quenching (qE) of excess light involves a ΔpH-induced activation of the xanthophyll cycle in which a violaxanthin de-epoxidase converts violaxanthin (V) into antheraxanthin (A) and zeaxanthin (Z)...
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Báo cáo y học: " Diametrically opposed effects of hypoxia and oxidative stress on two viral transactivators" pdf
Ngày tải lên :
12/08/2014, 04:20
... to increase radical generation and thereby circumvent the difficulty in inducing oxidative stress by solely increasing pO2 [49] Menadione and paraquot are most frequently used to increase intracellular ... epitope-tagged EBNA1 and Tat (A) Diagrams of epitope-tagged EBNA1 and Tat EBNA1 is 641 a.a long and binds 20 sites in the EBV FR through its DNA binding domain (DBD) EBNA1's UR1 domain, essential for ... shown in Figure 1A, emphasizing the domains of these two proteins that are required to bind their cognate recognition sites on DNA or RNA, and the domains that are redox-responsive EBNA1's DNA-binding...
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Báo cáo khoa học: " Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis" ppt
Ngày tải lên :
13/08/2014, 10:20
... adhesion involves the interaction of leukocyte integrins of the beta subfamily (CD11a, CD11b and CD11c) with endothelial ligand receptors [19] An increase in the expression of those integrins on ... neutrophils and monocytes in sepsis is of increasing interest Once primed by bacterial products and endogenous mediators [14-16], neutrophils adhere to activated cells in inflamed tissues and transmigrate ... Care Vol 12 No Martins et al The pathogenesis of sepsis involves a complex interaction between host and infecting microorganism, including bacterial recognition, cell activation and transmigration,...
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Báo cáo y học: "Road traffic accidents and posttraumatic stress disorder in an orthopedic setting in south-eastern Nigeria: a controlled study" doc
Ngày tải lên :
13/08/2014, 23:20
... (X2 = 20.09, df = 1, p = 0.000) Gainfully employed prior to the RTAs was defined by the individual concerned having a source of income and independent; either being self employed, employed by the ... occupation and the time since the road traffic accidents occurred among the study subjects Mini International Neuropsychiatric Interview (M.I.N.I)[10] This is a short structured interviewer administered ... participants in the first control group and one hundred and fifty (150) participants in the second control group selected from staffs of FNHE and NOHE, Nigeria who had not been involved in RTAs Socio-demographic...
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