screening of acid zeolites in vapor phase synthesis of ethers
Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
... Fig Combined effect of triacsin C and NDGA on steroid hormone synthesis Y1 cells were preincubated in the presence or the absence of increasing concentrations of triacsin C (4 h, 37 °C) and of NDGA ... inactive [49] In our experiments NDGA was effective in inhibiting the activity of purified recombinant MTE-I in an in vitro assay performed in a Hepes-based buffer containing only KCl Information ... Neuman, I., Finkielstein, C.F & Podesta, E.J (1997) Involvement of arachidonic acid and the lipoxygenase pathway in mediating luteinizing hormone-induced testosterone synthesis in rat Leydig...
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Báo cáo khoa học: Inhibition of aryl acid adenylation domains involved in bacterial siderophore synthesis ppt
... structural information, by comparing models of the aryl acid binding pockets of the two domains Results and Discussion Inhibition of DhbE with SAL-AMS and a set of new modified inhibitors In order ... groups (striped) are indicated (C) Alignment of DhbE and YbtE amino acids forming the aryl substrate binding pockets The differing amino acids are indicated by asterisks The amino acids with the highest ... amino acid residues forming the aryl acid binding pockets according to the nonribosomal code of A domain specificity [7,14] Therefore, a model of a putative binding pocket valid for SAL activating...
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Báo cáo hóa học: " Aqueous-Phase Synthesis of Silver Nanodiscs and Nanorods in Methyl Cellulose Matrix: Photophysical Study and Simulation of UV–Vis Extinction Spectra Using DDA Method" docx
... concentric ring with intermittent bright dots, indicating that the samples are highly crystalline in nature A closer look on the SAED pattern of Fig 1b(ii) suggests that the ring having d-values ... tuned by varying the concentration of tri-sodium citrate in the growth (Scheme 1) solution The seed particles consist of a mixture of single crystal and twinned crystals HR-TEM analysis of the green ... above seeding growth approach Our present simulation of extinction spectra using DDA calculation suggests the potentiality of DDA methodology while calculating the extinction spectra of anisotropically...
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Screening and evaluation of the anticancer potential of scorpion venoms and snake venom l amino acid oxidase in gastric cancer
... gastric carcinogenesis , genes mainly involved in the progression of intestinal GC; , genes involved in both intestinal and diffuse GC; , genes mainly involved in diffuse GC ↑ indicates overexpression ... toxins An increasing number of studies have shown the anticancer effects of venoms and toxins of snakes, scorpions and others in vitro and in vivo, which were achieved mainly through the inhibition ... of NUGC-3 xenograft model 55 2.4.2 Intratumoral injection of venom 56 2.4.3 Tissue processing, paraffin embedding and microtome sectioning 56 2.4.4 Haematoxylin and Eosin staining...
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Construction of bacterial artificial chromosome library for kineosphaera limosa strain lpha5t and screening of genes involved in polyhydroxyalkanoate synthesis
... CONSTRUCTION OF BACTERIAL ARTIFICAL CHROMOSOME LIBRARY FOR Kineosphaera limosa STRAIN Lpha5T AND SCREENING OF GENES INVOLVED IN POLYHYDROXYALKANOATE SYNTHESIS JI ZHIJUAN (M Sci NANKAI UNIVERSITY, CHINA) ... SUBMITTED FOR DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CIVIL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2005 DEDICATION Dedication to my father, Ji Lieshun, mother Li XueQin and my son Liu ... transfers a segment of chain in α-1,6 position 2.3.3 Genes encoding enzymes involving in glycogen biosynthesis Table 2.4 lists the genes encoding the enzymes involved in glycogen synthesis Genes glgC,...
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Acetic Acid Bacteria - screening of LAB strains
... transferred in MRS agar slants into 10 ml MRS broths and incubated for 24h at 30°C A loopful of each of these cultures was transferred into 100 ml conical flasks containing 10ml of mESM broth ... described in Bergey’s Manual [15] and the Prokaryotes [16] All the strains were maintained by weekly sub culturing from 48hrs MRS agar cultures The cultures were examined microscopically by staining ... Salt tolerance was assessed after 3days of incubation at concentration of 40 and 65 g lG1 NaCl in MRS broth Production of ammonia from arginine was done according to the method described by AbdEL-Malek...
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INFLUENCE DISTRIBUTIONS OF ACID DEPOSITION IN MOUNTAINOUS STREAMS ON A TALL CONE-SHAPED ISLAND, YAKUSHIMA
... until being washed off in the next rain In particular, the mass of accumulated Cl- and SO42- on the leaves was higher in November than in February (Fig 3) Although the concentrations of inorganic ... of direct runoff of ordinary storms As all mountain streams were composed mostly of groundwater runoff, their capacity to neutralize acid deposition could be estimated as the values of alkalinity ... main axis of a catchment and the prevailing wind govern the influence of acid deposition on the water quality of branch streams These features are caused mainly by the shape and isolation of...
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Tài liệu THE ROLES OF AMINO ACID CHELATES IN ANIMAL NUTRITION pdf
... Adenylate kinase Creatin kinase Arginine kinase 1,3-Diphosphoglycerate -> ADP-Transphosphatase Pyruvate kinase Hexokinase 6-Phosphofructokinase Galactokinase Phosphoglucokinase Gluconokinase Ribokinase ... nutrient including certain vitamins, amino acids and fats, any of which could decrease absorption; (c) any condition which increases the rate of passage of the minerals through the intestine, such ... result of increased bioavailability of these chelated forms v VI Introduction Of novel interest are the reports showing a protein sparing as a result of amino acid chelate supp1ementat ion In the...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents for glutamine (C), glutamic acid (D), asparagine (E), aspartic acid (F), alanine ... serine and glycine was reduced from 33% in the light to 25% in the dark (Fig 7A) Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino acids) ... detected for GDH in both the synthesis and deamination of glutamate (Fig 3F) In vitro activities of nitrogen assimilation into amino acids The in vitro activities of the key enzymes of nitrogen assimilation...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... initiation of synthesis of the viral genome In this report we investigated the template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further ... and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Fig Secondary structure of the 3¢ terminal sequences of HCV minus-strand RNA Model structures of domain I (A) and domain II (B, C and ... deletion of SL-B1 In contrast to the results obtained when changes were introduced in SL-A1, none of the modifications in SL-B1 reduced RNA synthesis (Table 1) In all cases RNA synthesis was increased...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... protein was organized from its polypeptide chain into N-terminal and C-terminal centers during refolding [21] Since then, many scientists have been interested in demonstrating this concept by using ... structure of the protein disintegrated Using the peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease in helicity ... maintained by other interactions such as local stable segments interacting around E75 and E129, areas with oppositely charged amino acids The truncated protein W140O in this study (deletion of...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps Residues present in the flexible region of the C-terminal extension of each of the eight ... that the binding of target proteins occurs in the groove between monomers, and involves a b-sheet region located at the beginning of the a-crystallin domain corresponding to residues 70–88 in aA-crystallin ... compared with the wild-type protein indicates that the structure of the N-terminal domain is maintained, at least in the vicinity of the tryptophan residues The increase in exposed clustered hydrophobicity...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles in generating these ... destroyed In addition to E75 forming a local stable area in SNase, in this study we searched for the existence of other local stable segments that may play similar key roles in sustaining protein native ... fluorescence intensity of W140 reflect a change in the surrounding hydrophobic environment of the tryptophan and thus indicate a change in the overall (tertiary) structure of the protein [15] Figure...
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... structures of b-amino acids overlap the canonical structures of a-amino acids? Subsequently, is it possible to substitute one a-amino acid in the sequence of a linear peptide for one b-amino acid without ... cause steric hindrance and does not confine orientations of the side chain to regions of space different from those permitted in the a-amino acid Thus, insertion of a single b-amino acid in a bioactive ... consequences of a single b-amino acid incorporation When Phe7 or Phe8 are replaced by b2-HPhe, the corresponding analogues are weak competitors of specific NK-1 binding sites These amino acids are in the...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... McAllister 87 In Vitro Screening of Feed Resources for Efficiency of Microbial Protein Synthesis Harinder P.S Makkar 107 Screening of Plants for Inhibitory Activity Against Pathogenic ... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
... contribution of these amino acids interacting with the pyrimidine ring to the catalysis of E coli CMP kinase Substitution of the side chain from a well-structured protein can have two types of consequence: ... different time intervals, aliquots were withdrawn and diluted in buffer containing 10 lgặmL)1 of soybean trypsin inhibitor The rst-order rate constant of inactivation of CMP kinase by TPCK-trypsin was ... proportion of dimers were formed, even in the case of the wild-type protein as indicated by ESI-MS or SDS PAGE in the absence of reducing agents The proportion of dimers increased notably in D132A...
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Photoluminescence and growth mechanism of amorphous silica nanowires by vapor phase transport
... etching of substrate It is worth mentioning that, although both ZnOx and SiClx are in vapor phase, SiClx is an intermediate phase of a series of chemical reactions, and it oxidized quickly into ... white fog may contain SiOx, Cl2, HCl, ZnOx, ZnCl2, and zinc oxychloride, with little amount of VO2 Since vapor pressure of VO2 is low even at high temperature, and the melting point of VO2 is 1967 ... oxidation of ZnCl2, and maintain a pressure of 2–0.3 Pa in the tube The morphology, size, and crystal structure, of the SiONWs were determined using a cold cathode field emission scanning electron...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... biosynthesis systems of gramicidin S (Grs), tyrocidin A (Tyc), surfactin (Srf), lichenysin (Lic), bacitracin (Bac), fengycin (Fen), enterobactin (Ent), chloroeremomycin (Cep), pristinamycin (Snb), ... chromosomal point mutation in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthetase was found to abolish surfactin production in the B subtilis ... C-domains possess an intrinsic editing function for the incoming aminoacyl moiety [6] By using aminoacyl-S-CoA as probes it was shown that the ®rst C-domain of the tyrocidine synthetase complex...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
... 27 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 amino amino amino amino amino 15 15 15 15 15 acids acids acids acids acids amino amino amino amino amino acids ⁄ 237 acids ⁄ 237 acids ... Obtaining 5¢ and 3¢ ends by Rapid Amplification of cDNA Ends (RACE) amino amino amino amino amino acids acids acids acids acids Relative molecular mass ⁄ pI of mature protein homology search in ... trypsin (PDB code: 2f91A) showing the difference in superficial loops the N-terminal domain of bovine trypsin is also absent in lobster From the analysis of the crystal structure of crayfish trypsin...
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Báo cáo khoa học: Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP+-dependent malic enzyme docx
... examine the contribution of these three amino -acid residues to metal ion binding and enzymatic catalysis In this study, we sought to delineate the possible roles of these conserved residues in ... 5463–5467 Mechanism of malic enzyme 34 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem ... position of Mn2+ relative to the other amino -acid residues responsible for its binding A significant decrease in the kcat value of the D235A mutant has not been reported previously The carboxy group of...
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